Rmu_sc0001381.1_g000014

Phytoene synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001381.1
OG0016301
Physical Location & Seq
Forward (+)
42772 .. 44690
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1125 bp
atgagttctacattttcacttgcaccaaaaccttacattagagagaacaatggaaaactcctgcccccaaaatctatgctcacaacaattagagctgaagtgattacagctcccaagcaaagaagcacgcggatttttcctgaactgtcaatacaaggcattcctgttgctgatttgcatgtacaagaaattgttcaaaggcaatccaagacttacaacacagccaatggaggtactagccgaaagccacagttcgatccatgctttcttgaagaagcgtatgaaaggtgcaggaccatctgtgcagaatatgccaagacattctatctaggcactttgctaatgacagaggagcgtcaaaaggcaatatgggcaatctatgtttggtgcagaagaacagatgaactggttgatggccccaattctgggcacatggactctgaagttcttgatagatgggaagagagactagaagacattttcgaaggacggccttatgacatgcttgatgctgcattgactcatactgttctcaacttccccttagacattaagggtatgcgaatggacacaagaaaatgccgatatcagaattttcaagagctttatctctattgctactatgtggctggaactgttggcctaatgagtgttcctgtaatgggaattgcaccagattctctgatttctgctcaaagtacatatgatgctgcactctacttgggtatagggaatcaactcacaaacatacttagagatgtcggggaggatactgcgagaggtagagtttatcttccccaagatgagctggcacagtttgggttatccgacgacgatgttttttcaagaaaggtcactgatcaatggagagagttcatgaaagaacagattaccagagcgagattctatttcaacctggcagaggagggtgcttctcaacttgaaaaggatagccgttggccggtatggtcatcgttgctgatatatcgaaacatcttggatgcaattgaggaaaataattacgataacttgacaaagagagcatatgtaggcagagctaagaaacttctcatgttgcctttggcttacacaaaatcccttaatacacatggtctgatactttga

Protein Analysis

374

Amino Acids

43.04

Weight (kDa)

5.78

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQS_PSY PF00494 103 - 352 1.2e-64 Squalene/phytoene synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016301)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45220
malus_domestica MD03G1007200.v1.1 MD11G1010500.v1.1
prunus_persica Prupe.6G006800_v2.0.a1
pyrus_communis pycom03g00730
rosa_chinensis RchiOBHm_Chr5g0082831
rosa_laevigata RLG00000036993
rosa_multiflora Rmu_sc0001381.1_g000014
rosa_roxburghii Rroxscaffold_1G00001600
rosa_rugosa Rorug05G0480200
rosa_samantha Rh5AG530500 Rh5BG557400 Rh5CG580400 Rh5DG565800
rosa_wichuraiana Rw5G049810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 130
AciI CCGC 1 cut(s) 130
AclWI GGATC 1 cut(s) 251
AcoI YGGCCR 1 cut(s) 959
AcsI RAATTY 1 cut(s) 592
AcuI CTGAAG 2 cut(s) 117, 462
AfaI GTAC 3 cut(s) 183, 235, 700
AfiI CCNNNNNNNGG 5 cut(s) 425, 426, 662, 922, 961
AgsI TTSAA 6 cut(s) 197, 272, 599, 846, 913, 944
AjnI CCWGG 1 cut(s) 915
AluBI AGCT 5 cut(s) 95, 110, 604, 808, 1058
AluI AGCT 5 cut(s) 95, 110, 604, 808, 1058
Alw26I GTCTC 1 cut(s) 460
AlwI GGATC 1 cut(s) 251
AoxI GGCC 4 cut(s) 415, 491, 640, 959
ApeKI GCWGC 2 cut(s) 512, 710
ApoI RAATTY 1 cut(s) 592
Asp700I GAANNNNTTC 1 cut(s) 192
AspS9I GGNCC 2 cut(s) 294, 416
AsuII TTCGAA 1 cut(s) 483
AvaII GGWCC 1 cut(s) 294
BaeGI GKGCMC 1 cut(s) 432
BbsI GAAGAC 1 cut(s) 480
BbvI GCAGC 2 cut(s) 499, 697
BccI CCATC 3 cut(s) 305, 407, 450
BceAI ACGGC 2 cut(s) 506, 939
BciT130I CCWGG 1 cut(s) 917
BciVI GTATCC 1 cut(s) 763
BclI TGATCA 1 cut(s) 859
BcoDI GTCTC 1 cut(s) 460
BfaI CTAG 3 cut(s) 237, 329, 470
BfuI GTATCC 1 cut(s) 763
BisI GCNGC 2 cut(s) 513, 711
BlsI GCNGC 2 cut(s) 514, 712
Bme1390I CCNGG 1 cut(s) 917
Bme18I GGWCC 1 cut(s) 294
BmgT120I GGNCC 2 cut(s) 294, 416
BmiI GGNNCC 1 cut(s) 418
BmrFI CCNGG 1 cut(s) 917
BmsI GCATC 3 cut(s) 499, 697, 991
BpiI GAAGAC 1 cut(s) 480
Bpu14I TTCGAA 1 cut(s) 483
Bsc4I CCNNNNNNNGG 5 cut(s) 425, 426, 662, 922, 961
Bse118I RCCGGY 1 cut(s) 961
Bse1I ACTGG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 917
BseGI GGATG 1 cut(s) 1006
BseLI CCNNNNNNNGG 5 cut(s) 425, 426, 662, 922, 961
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 2 cut(s) 365, 938
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 2 cut(s) 499, 697
BsgI GTGCAG 4 cut(s) 310, 324, 409, 696
Bsh1236I CGCG 1 cut(s) 130
BshFI GGCC 4 cut(s) 417, 493, 642, 961
BsiSI CCGG 1 cut(s) 962
BslI CCNNNNNNNGG 5 cut(s) 425, 426, 662, 922, 961
BsmAI GTCTC 1 cut(s) 460
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 4 cut(s) 417, 493, 642, 961
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 432
Bsp1407I TGTACA 1 cut(s) 181
Bsp143I GATC 2 cut(s) 256, 859
BspACI CCGC 1 cut(s) 130
BspANI GGCC 4 cut(s) 417, 493, 642, 961
BspFNI CGCG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 876
BspLI GGNNCC 1 cut(s) 418
BspPI GGATC 1 cut(s) 251
BspT104I TTCGAA 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 961
BsrGI TGTACA 1 cut(s) 181
BsrI ACTGG 1 cut(s) 411
BssAI RCCGGY 1 cut(s) 961
BssMI GATC 2 cut(s) 256, 859
Bst2UI CCWGG 1 cut(s) 917
Bst4CI ACNGT 5 cut(s) 147, 252, 529, 637, 816
Bst6I CTCTTC 1 cut(s) 456
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 181
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 2 cut(s) 128, 810
BstDEI CTNAG 3 cut(s) 544, 752, 1059
BstENI CCTNNNNNAGG 1 cut(s) 920
BstF5I GGATG 1 cut(s) 1006
BstFNI CGCG 1 cut(s) 130
BstKTI GATC 2 cut(s) 259, 862
BstMAI GTCTC 1 cut(s) 460
BstMBI GATC 2 cut(s) 256, 859
BstMWI GCNNNNNNNGC 2 cut(s) 311, 371
BstNI CCWGG 1 cut(s) 917
BstNSI RCATGY 2 cut(s) 182, 505
BstSCI CCNGG 1 cut(s) 915
BstSLI GKGCMC 1 cut(s) 432
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 2 cut(s) 499, 697
BstV2I GAAGAC 1 cut(s) 480
BsuI GTATCC 1 cut(s) 763
BsuRI GGCC 4 cut(s) 417, 493, 642, 961
BtsCI GGATG 1 cut(s) 1006
BtsIMutI CAGTG 1 cut(s) 855
Cac8I GCNNGC 2 cut(s) 128, 810
CciI TCATGA 1 cut(s) 876
Cfr10I RCCGGY 1 cut(s) 961
Cfr13I GGNCC 2 cut(s) 294, 416
CseI GACGC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 182, 234, 699
CviAII CATG 7 cut(s) 179, 261, 433, 502, 877, 1072, 1109
CviQI GTAC 3 cut(s) 182, 234, 699
DdeI CTNAG 3 cut(s) 544, 752, 1059
DpnI GATC 2 cut(s) 258, 861
DpnII GATC 2 cut(s) 256, 859
EaeI YGGCCR 1 cut(s) 959
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco32I GATATC 1 cut(s) 587
Eco47I GGWCC 1 cut(s) 294
Eco57I CTGAAG 2 cut(s) 117, 462
EcoNI CCTNNNNNAGG 1 cut(s) 920
EcoRII CCWGG 1 cut(s) 915
EcoRV GATATC 1 cut(s) 587
FaeI CATG 7 cut(s) 182, 264, 436, 505, 880, 1075, 1112
FatI CATG 7 cut(s) 178, 260, 432, 501, 876, 1071, 1108
FauNDI CATATG 2 cut(s) 703, 1045
FbaI TGATCA 1 cut(s) 859
Fnu4HI GCNGC 2 cut(s) 513, 711
FokI GGATG 1 cut(s) 1013
Fsp4HI GCNGC 2 cut(s) 513, 711
FspBI CTAG 3 cut(s) 237, 329, 470
GluI GCNGC 2 cut(s) 513, 711
HaeIII GGCC 4 cut(s) 417, 493, 642, 961
HapII CCGG 1 cut(s) 962
HgaI GACGC 1 cut(s) 344
Hin1II CATG 7 cut(s) 182, 264, 436, 505, 880, 1075, 1112
HinfI GANTC 5 cut(s) 437, 520, 677, 733, 903
HpaII CCGG 1 cut(s) 962
Hpy188I TCNGA 5 cut(s) 442, 591, 684, 829, 1116
Hpy188III TCNNGA 6 cut(s) 140, 269, 449, 599, 846, 877
Hpy99I CGWCG 2 cut(s) 833, 836
HpyAV CCTTC 1 cut(s) 479
HpyCH4III ACNGT 5 cut(s) 147, 252, 529, 637, 816
HpyCH4V TGCA 9 cut(s) 23, 178, 291, 305, 390, 515, 671, 713, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 371
HpyF3I CTNAG 3 cut(s) 544, 752, 1059
Hsp92II CATG 7 cut(s) 182, 264, 436, 505, 880, 1075, 1112
Ksp22I TGATCA 1 cut(s) 859
Kzo9I GATC 2 cut(s) 256, 859
LmnI GCTCC 2 cut(s) 115, 352
Lsp1109I GCAGC 2 cut(s) 499, 697
LweI GCATC 3 cut(s) 499, 697, 991
MaeI CTAG 3 cut(s) 237, 329, 470
MaeIII GTNAC 1 cut(s) 853
MalI GATC 2 cut(s) 258, 861
MboI GATC 2 cut(s) 256, 859
MboII GAAGA 5 cut(s) 284, 405, 473, 485, 785
MfeI CAATTG 1 cut(s) 1005
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 7 cut(s) 87, 189, 421, 592, 666, 1005, 1018
MlyI GAGTC 2 cut(s) 431, 514
MmeI TCCRAC 1 cut(s) 852
MnlI CCTC 7 cut(s) 224, 343, 760, 773, 916, 919, 1003
MroXI GAANNNNTTC 1 cut(s) 192
MseI TTAA 2 cut(s) 552, 1101
MspI CCGG 1 cut(s) 962
MspR9I CCNGG 1 cut(s) 917
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 1 cut(s) 159
MvaI CCWGG 1 cut(s) 917
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 2 cut(s) 311, 371
NdeI CATATG 2 cut(s) 703, 1045
NdeII GATC 2 cut(s) 256, 859
NlaIII CATG 7 cut(s) 182, 264, 436, 505, 880, 1075, 1112
NlaIV GGNNCC 1 cut(s) 418
NmuCI GTSAC 1 cut(s) 853
NspI RCATGY 2 cut(s) 182, 505
NspV TTCGAA 1 cut(s) 483
PagI TCATGA 1 cut(s) 876
PctI GAATGC 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 3 cut(s) 677, 733, 903
PkrI GCNGC 2 cut(s) 514, 712
PleI GAGTC 2 cut(s) 431, 514
PpsI GAGTC 2 cut(s) 431, 514
Psp6I CCWGG 1 cut(s) 915
PspGI CCWGG 1 cut(s) 915
PspN4I GGNNCC 1 cut(s) 418
PspPI GGNCC 2 cut(s) 294, 416
PsrI GAACNNNNNNTAC 2 cut(s) 135, 167
RsaI GTAC 3 cut(s) 183, 235, 700
RsaNI GTAC 3 cut(s) 182, 234, 699
SaqAI TTAA 2 cut(s) 552, 1101
SatI GCNGC 2 cut(s) 513, 711
Sau3AI GATC 2 cut(s) 256, 859
Sau96I GGNCC 2 cut(s) 294, 416
SchI GAGTC 2 cut(s) 431, 514
ScrFI CCNGG 1 cut(s) 917
SduI GDGCHC 1 cut(s) 432
SfaNI GCATC 3 cut(s) 499, 697, 991
SfuI TTCGAA 1 cut(s) 483
SinI GGWCC 1 cut(s) 294
Sse9I AATT 7 cut(s) 87, 189, 421, 592, 666, 1005, 1018
SsiI CCGC 1 cut(s) 130
SspMI CTAG 3 cut(s) 237, 329, 470
StyD4I CCNGG 1 cut(s) 915
TaaI ACNGT 5 cut(s) 147, 252, 529, 637, 816
TaqI TCGA 3 cut(s) 255, 483, 988
TasI AATT 7 cut(s) 87, 189, 421, 592, 666, 1005, 1018
TatI WGTACW 2 cut(s) 181, 698
TfiI GAWTC 3 cut(s) 677, 733, 903
Tru1I TTAA 2 cut(s) 552, 1101
Tru9I TTAA 2 cut(s) 552, 1101
TscAI CASTG 1 cut(s) 862
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 2 cut(s) 512, 710
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 4 cut(s) 297, 417, 865, 893
TspRI CASTG 1 cut(s) 862
VpaK11BI GGWCC 1 cut(s) 294
XagI CCTNNNNNAGG 1 cut(s) 920
XapI RAATTY 1 cut(s) 592
XceI RCATGY 2 cut(s) 182, 505
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 3 cut(s) 237, 329, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.