Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001874.1

Orthogroup ID

OG0014584

Physical Location & Seq

Genomic Coordinates Forward (+)

9573 .. 24427

Gene Structure 14855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3663 bp

atggtggcggcgccgcccaagtacgtgagctcagtaggcgatctgaagcgccgcgagttgcagaagaagaaagccaagtctggagggttcgagtctctcaacctaagccccaatgtcttcaaagcaatcaagcgcaagggctacaaagtccccacacccattcagcgcaagaccatgccccttattctctccggaaacgacgtcgtcgccatggcccgaaccggttccggcaaaacggcggcgtttctcatccccatgctcgagcgtttgaaggagcacgtcccgcagggcggtgttagggctctcattctctcccccaccagagacttggctcttcagaccctcaagtttaccacagagctcgcccaattcatggatcttcggattagtttactagttggtggtgatagtatggaaactcagtttgaggagctggctcaaaacccggatattataattgcaacacctggcaggctgattcaccatttaactgagatcgatgagatgtcgcttcgctcggtggagtatgttgtttttgatgaggccgattgtctctttggcatgggttttgctgagcagttgcataagattcttgcgcagctgagtgataaccgtcagacattgctctttagtgcaacattacctagcgcgctcgcagagtttgcgaaggctggtctgcaagaccctcgccttgtgcggcttgatttggatactaagatcagccccgacttgaagctgatgtttttcaccttgcgacaggaagagaagcatgcggcaatactttatttggtaagggagcatattcgttccgatgagcagacgttgatatttgtatccactaagcatcatgtggagttcctgaatatcttgtttagagaagagggcattgtgccttctgtgtgttatggtgacatggatcatgatgctcggaaaattcatatatcaaagtttaagcacagaaagactatgttgttaattgttactgatgttgcagccaggggaattgatattccattgcttgacaatgttatcaattgggattttcccccaaagcctaaactttttgtccatagagtaggtcgagctgcaagggctggtcggactggcactgcgttctctcttgtgacatctgaagacatacctaaccttttagatcttcatctgtttctttcaaaaccgattaggccagcacctacggaggaggaggttttacaggatatggatggagtaatgtccaagattgatcaagcagttgcaaatggagaaagtatatatgggcgtttcccccaaacagctattgatctggtttcagacagggttagagagataattgattcttcttcagaactgagccttttgctgaaaacttgcgcaaatgcatttcgcttgtattcaaagacaaagccctccccttcaagggaatctattaaaagagcaaaagatttacctcgggaagggttgcatccaatatttaaaaacacacttgagggtggagagttgaaggcattggcattttctgaacggttgaagacattcagacctgagcagaccattctagaagctgaagggaaagctgctaagtcaaagaatgtgaagggtccttctcgtcagtgggttgatgtaatgaagaaaaaaagagctattcatgaagggataattagtttagtgcatcagcaaaatcaggaacgttccaataatcatgctgaagaggaagatgaatctgagattattcccttaaagaaaaagagaaagaaagaatcttctggttctaaaaggaaggcaacgagcttcaaggatgaggattactttataagttccatacccacaaatcatcatatggaagcgggactctcagtgagagggaatgagaactttgactctaataggttggaagctgctgttctagatctagttgcagatgataatgtgggcatgaagaaacagaaatctgtatttcattgggataagagtgggaaaaagtacataaagctaacttcacgcgaccgagtgactgctagcggcaaggtaaagacagagagtggtgccaaaggaaaacttgagaaaactggaatatacaataagtggaaagcacgttcacacaataaagtatctttcgaaggcactgacgaagggaatgctgaggaagccacaggcaaccggagatggcaggggaacagggggaacccacggggaggcaggaagcggcagtctattcctaatgctcatgtgcgttctgaaatcaaagacttggatcaagctcaggcaataatggtcaaagaaactgagtactatgatgcactcaatgtcagtccctcggcttcggaagaggaaattcgcaaggcttattatctcaaggcaaggcaagtacatccggataaaaatccaaatgatcctcaagctgctgaaagatttcaggtacttggggaagcctaccaagttttgagtgatccactgcaaagagaagcatatgatcgaaatggaaagcactgcatatctagggatacaatgcttgaccccacagctgtctttgcttttcttttcggaagtgaactatttgaggattacatagggcatctagctgtagcatcaatggcttcttctcaattagctggtgaaggtgacaaccctgagaaagtacatgataaattgaaggctgtccagaaagaaagagaagaaaagctagctaaggcactcaaagatcttcttaatcagtatgtgcgaggtgataaagaagggttcttacttcatgctgaatctgaagctagaagactttctgatgcagcctttggaattgatatgctacacactattggctatgtttattcaagacaagcagcacaagagcttggaaagaaaaccatatacctaggggtaccatttttggctgagtgggctgtccagaaagaaagagaagaaaagctagctaaggcactcaaagatcttcttaatcagtatgtgcgaggtgataaagaagggttcttacttcatgctgaatctgaagctagaagactttctgatgcagcctttggaattgatatgctacacactattggctatgtttattcaagacaagcagcacaagagcttggaaagaaaaccatatacctaggggtaccatttttggctgagtgggtgcgcaacaagggacatttctggaagtcacagatgaccgctgcaaaaggtgcattccagctgatgcaacttcaagaagaaatccgtcggcagtttaaagtggatggtagtggccctgaacatgatgttgaatcacatatacgcttaaacaaagacacattattgaactcattgtggaaattgaatgtggttgacattgaagtaactctcgtacatgtttgtcaaatggtgctacgggaaactaatgttcgaaaggatgaacttagagctcgtgctttggcactaaagcttcttggaaagatatttcaggaggagaagcagacacaaaatggtgcaacttcaaaacggaaaggtgatgctgacacagacgatgatgatggaactagttctgatagtagtggagaggattcaccacaacggctttcatatagaactccttttctcactcagggcattggaagactcttcagatgcctctgtaatccagcatatgatgttgatgatgacgagattgtgtacaaaggcaaatga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DBP10CT DEAD Helicase_C

Protein Analysis

1220

Amino Acids

137.4

Weight (kDa)

8.82

Isoelectric Point (pI)

37.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	52 - 220	1.9e-50	DEAD/DEAH box helicase
Helicase_C	PF00271	258 - 366	2e-23	Helicase conserved C-terminal domain
DBP10CT	PF08147	636 - 697	4.1e-09	DBP10CT (NUC160) domain
DnaJ	PF00226	762 - 824	4.8e-24	DnaJ domain
DnaJ-X	PF14308	887 - 969	8.3e-20	X-domain of DnaJ-containing
DnaJ-X	PF14308	967 - 1159	7.4e-52	X-domain of DnaJ-containing

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77030
fragaria_vesca	FvH4_2g15160 FvH4_2g15160
malus_domestica	MD07G1108300.v1.1
prunus_persica	Prupe.2G131100_v2.0.a1 Prupe.2G131100_v2.0.a1
pyrus_communis	pycom07g09270
rosa_chinensis	RchiOBHm_Chr6g0277821
rosa_laevigata	RLG00000013266
rosa_multiflora	Rmu_sc0001874.1_g000004
rosa_roxburghii	Rroxscaffold_7G00190870
rosa_rugosa	Rorug06G0111700
rosa_samantha	Rh6AG222500 Rh6BG226000 Rh6CG228500 Rh6DG219600
rosa_wichuraiana	Rw6G019430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	455, 1811
AatII	GACGTC	1 cut(s)	204
Acc16I	TGCGCA	3 cut(s)	597, 1372, 3137
Acc65I	GGTACC	2 cut(s)	2872, 3112
AccB1I	GGYRCC	4 cut(s)	10, 2042, 2872, 3112
AccB7I	CCANNNNNTGG	1 cut(s)	373
AccII	CGCG	3 cut(s)	54, 650, 2001
AccIII	TCCGGA	2 cut(s)	191, 2371
AclI	AACGTT	1 cut(s)	1687
AclWI	GGATC	5 cut(s)	384, 924, 2259, 2384, 2441
AcsI	RAATTY	2 cut(s)	933, 2331
AcuI	CTGAAG	9 cut(s)	65, 320, 1152, 1326, 1584, 1725, 2778, 3018, 3583
AcyI	GRCGYC	2 cut(s)	11, 201
AfiI	CCNNNNNNNGG	6 cut(s)	290, 373, 1416, 1417, 1454, 2192
AflIII	ACRYGT	1 cut(s)	3346
AgeI	ACCGGT	1 cut(s)	221
AhlI	ACTAGT	2 cut(s)	394, 3515
AjiI	CACGTC	1 cut(s)	280
AjnI	CCWGG	2 cut(s)	466, 995
AloI	GAACNNNNNNTCC	2 cut(s)	2077, 2109
Alw21I	GWGCWC	4 cut(s)	32, 279, 363, 3403
Alw26I	GTCTC	3 cut(s)	99, 318, 557
AlwI	GGATC	5 cut(s)	384, 924, 2259, 2384, 2441
Ama87I	CYCGRG	2 cut(s)	260, 1449
Aor13HI	TCCGGA	2 cut(s)	191, 2371
AoxI	GGCC	4 cut(s)	213, 543, 1184, 3244
ApoI	RAATTY	2 cut(s)	933, 2331
AsiGI	ACCGGT	1 cut(s)	221
Asp700I	GAANNNNTTC	6 cut(s)	223, 1532, 2409, 2770, 3010, 3517
Asp718I	GGTACC	2 cut(s)	2872, 3112
AspA2I	CCTAGG	2 cut(s)	2866, 3106
AspLEI	GCGC	9 cut(s)	13, 51, 135, 168, 598, 650, 652, 1373, 3138
AspS9I	GGNCC	3 cut(s)	214, 1598, 3245
AsuC2I	CCSGG	1 cut(s)	446
AsuII	TTCGAA	2 cut(s)	2115, 3382
AsuNHI	GCTAGC	3 cut(s)	2015, 2680, 2920
AvaI	CYCGRG	2 cut(s)	260, 1449
AvaII	GGWCC	1 cut(s)	1598
AvrII	CCTAGG	2 cut(s)	2866, 3106
BanI	GGYRCC	4 cut(s)	10, 2042, 2872, 3112
BanII	GRGCYC	4 cut(s)	32, 304, 363, 3403
BarI	GAAGNNNNNNTAC	4 cut(s)	2724, 2756, 2964, 2996
BauI	CACGAG	1 cut(s)	3402
BbsI	GAAGAC	6 cut(s)	109, 1140, 1535, 2773, 3013, 3598
Bbv12I	GWGCWC	4 cut(s)	32, 279, 363, 3403
BbvCI	CCTCAGC	1 cut(s)	2139
BccI	CCATC	4 cut(s)	1217, 2157, 3230, 3503
BceAI	ACGGC	2 cut(s)	252, 3566
BcgI	CGANNNNNNTGC	4 cut(s)	668, 702, 2117, 2151
BciT130I	CCWGG	2 cut(s)	468, 997
BciVI	GTATCC	3 cut(s)	703, 844, 2494
BclI	TGATCA	1 cut(s)	1243
BcnI	CCSGG	1 cut(s)	446
BcoDI	GTCTC	3 cut(s)	99, 318, 557
BcuI	ACTAGT	2 cut(s)	394, 3515
BfmI	CTRYAG	1 cut(s)	2580
BfoI	RGCGCY	2 cut(s)	14, 52
BfuI	GTATCC	3 cut(s)	703, 844, 2494
BglII	AGATCT	4 cut(s)	1153, 1906, 2698, 2938
BlnI	CCTAGG	2 cut(s)	2866, 3106
BlpI	GCTNAGC	1 cut(s)	573
BmcAI	AGTACT	1 cut(s)	2288
Bme1390I	CCNGG	3 cut(s)	446, 468, 997
Bme18I	GGWCC	1 cut(s)	1598
BmeT110I	CYCGRG	2 cut(s)	260, 1449
BmgBI	CACGTC	1 cut(s)	280
BmgT120I	GGNCC	3 cut(s)	214, 1598, 3245
BmiI	GGNNCC	7 cut(s)	12, 226, 1599, 2044, 2183, 2874, 3114
BmrFI	CCNGG	3 cut(s)	446, 468, 997
BmtI	GCTAGC	3 cut(s)	2019, 2684, 2924
BpiI	GAAGAC	6 cut(s)	109, 1140, 1535, 2773, 3013, 3598
BpmI	CTGGAG	1 cut(s)	102
Bpu10I	CCTNAGC	6 cut(s)	104, 1542, 2139, 2259, 2685, 2925
Bpu1102I	GCTNAGC	1 cut(s)	573
Bpu14I	TTCGAA	2 cut(s)	2115, 3382
BpuEI	CTTGAG	5 cut(s)	329, 1505, 2078, 2336, 2379
BpuMI	CCSGG	1 cut(s)	446
Bsa29I	ATCGAT	1 cut(s)	498
BsaAI	YACGTR	1 cut(s)	25
BsaBI	GATNNNNATC	2 cut(s)	1158, 2379
BsaHI	GRCGYC	2 cut(s)	11, 201
BsaJI	CCNNGG	7 cut(s)	210, 996, 1448, 2186, 2313, 2866, 3106
BsaWI	WCCGGW	4 cut(s)	191, 221, 2157, 2371
Bsc4I	CCNNNNNNNGG	6 cut(s)	290, 373, 1416, 1417, 1454, 2192
Bse118I	RCCGGY	1 cut(s)	221
Bse1I	ACTGG	2 cut(s)	1108, 2071
Bse3DI	GCAATG	2 cut(s)	620, 1013
Bse8I	GATNNNNATC	2 cut(s)	1158, 2379
BseAI	TCCGGA	2 cut(s)	191, 2371
BseBI	CCWGG	2 cut(s)	468, 997
BseCI	ATCGAT	1 cut(s)	498
BseDI	CCNNGG	7 cut(s)	210, 996, 1448, 2186, 2313, 2866, 3106
BseGI	GGATG	7 cut(s)	249, 1228, 1462, 1801, 2368, 3241, 3394
BseJI	GATNNNNATC	2 cut(s)	1158, 2379
BseLI	CCNNNNNNNGG	6 cut(s)	290, 373, 1416, 1417, 1454, 2192
BseMI	GCAATG	2 cut(s)	620, 1013
BseNI	ACTGG	2 cut(s)	1108, 2071
BsePI	GCGCGC	1 cut(s)	648
BseRI	GAGGAG	4 cut(s)	443, 1214, 1217, 3458
Bsh1236I	CGCG	3 cut(s)	54, 650, 2001
Bsh1285I	CGRYCG	1 cut(s)	2005
BshFI	GGCC	4 cut(s)	215, 545, 1186, 3246
BshNI	GGYRCC	4 cut(s)	10, 2042, 2872, 3112
BshTI	ACCGGT	1 cut(s)	221
BshVI	ATCGAT	1 cut(s)	498
BsiEI	CGRYCG	1 cut(s)	2005
BsiHKAI	GWGCWC	4 cut(s)	32, 279, 363, 3403
BsiHKCI	CYCGRG	2 cut(s)	260, 1449
BsiSI	CCGG	6 cut(s)	192, 222, 228, 446, 2158, 2372
BslFI	GGGAC	5 cut(s)	134, 266, 1860, 2295, 3159
BslI	CCNNNNNNNGG	6 cut(s)	290, 373, 1416, 1417, 1454, 2192
BsmAI	GTCTC	3 cut(s)	99, 318, 557
BsmFI	GGGAC	5 cut(s)	134, 266, 1860, 2295, 3159
BsmI	GAATGC	2 cut(s)	2140, 3185
BsnI	GGCC	4 cut(s)	215, 545, 1186, 3246
BsoBI	CYCGRG	2 cut(s)	260, 1449
Bsp119I	TTCGAA	2 cut(s)	2115, 3382
Bsp1286I	GDGCHC	5 cut(s)	32, 279, 304, 363, 3403
Bsp13I	TCCGGA	2 cut(s)	191, 2371
Bsp1407I	TGTACA	1 cut(s)	3648
Bsp1720I	GCTNAGC	1 cut(s)	573
Bsp19I	CCATGG	1 cut(s)	210
BspANI	GGCC	4 cut(s)	215, 545, 1186, 3246
BspDI	ATCGAT	1 cut(s)	498
BspEI	TCCGGA	2 cut(s)	191, 2371
BspFNI	CGCG	3 cut(s)	54, 650, 2001
BspHI	TCATGA	2 cut(s)	919, 1645
BspLI	GGNNCC	7 cut(s)	12, 226, 1599, 2044, 2183, 2874, 3114
BspOI	GCTAGC	3 cut(s)	2019, 2684, 2924
BspPI	GGATC	5 cut(s)	384, 924, 2259, 2384, 2441
BspQI	GCTCTTC	1 cut(s)	339
BspT104I	TTCGAA	2 cut(s)	2115, 3382
BspT107I	GGYRCC	4 cut(s)	10, 2042, 2872, 3112
BsrDI	GCAATG	2 cut(s)	620, 1013
BsrFI	RCCGGY	1 cut(s)	221
BsrGI	TGTACA	1 cut(s)	3648
BsrI	ACTGG	2 cut(s)	1108, 2071
BssAI	RCCGGY	1 cut(s)	221
BssECI	CCNNGG	7 cut(s)	210, 996, 1448, 2186, 2313, 2866, 3106
BssHII	GCGCGC	1 cut(s)	648
BssNI	GRCGYC	2 cut(s)	11, 201
BssSI	CACGAG	1 cut(s)	3402
BssT1I	CCWWGG	3 cut(s)	210, 2866, 3106
Bst2BI	CACGAG	1 cut(s)	3402
Bst2UI	CCWGG	2 cut(s)	468, 997
Bst4CI	ACNGT	2 cut(s)	614, 1524
Bst6I	CTCTTC	6 cut(s)	339, 756, 873, 1701, 2319, 3602
BstACI	GRCGYC	2 cut(s)	11, 201
BstAPI	GCANNNNNTGC	2 cut(s)	662, 3182
BstAUI	TGTACA	1 cut(s)	3648
BstBAI	YACGTR	1 cut(s)	25
BstBI	TTCGAA	2 cut(s)	2115, 3382
BstDSI	CCRYGG	2 cut(s)	210, 2186
BstENI	CCTNNNNNAGG	1 cut(s)	1452
BstF5I	GGATG	7 cut(s)	249, 1228, 1462, 1801, 2368, 3241, 3394
BstFNI	CGCG	3 cut(s)	54, 650, 2001
BstH2I	RGCGCY	2 cut(s)	14, 52
BstHHI	GCGC	9 cut(s)	13, 51, 135, 168, 598, 650, 652, 1373, 3138
BstMAI	GTCTC	3 cut(s)	99, 318, 557
BstMCI	CGRYCG	1 cut(s)	2005
BstMWI	GCNNNNNNNGC	9 cut(s)	36, 283, 662, 1091, 2144, 2528, 2591, 2891, 3182
BstNI	CCWGG	2 cut(s)	468, 997
BstNSI	RCATGY	2 cut(s)	773, 3350
BstSCI	CCNGG	3 cut(s)	444, 466, 995
BstSFI	CTRYAG	1 cut(s)	2580
BstUI	CGCG	3 cut(s)	54, 650, 2001
BstV2I	GAAGAC	6 cut(s)	109, 1140, 1535, 2773, 3013, 3598
BstX2I	RGATCY	5 cut(s)	376, 1153, 1906, 2698, 2938
BstXI	CCANNNNNNTGG	1 cut(s)	328
BstYI	RGATCY	5 cut(s)	376, 1153, 1906, 2698, 2938
Bsu15I	ATCGAT	1 cut(s)	498
BsuI	GTATCC	3 cut(s)	703, 844, 2494
BsuRI	GGCC	4 cut(s)	215, 545, 1186, 3246
BsuTUI	ATCGAT	1 cut(s)	498
BtgI	CCRYGG	2 cut(s)	210, 2186
BtrI	CACGTC	1 cut(s)	280
BtsCI	GGATG	7 cut(s)	249, 1228, 1462, 1801, 2368, 3241, 3394
BtsI	GCAGTG	3 cut(s)	1107, 2450, 2485
BtsIMutI	CAGTG	6 cut(s)	1107, 1616, 1860, 2121, 2450, 2485
CciI	TCATGA	2 cut(s)	919, 1645
CfoI	GCGC	9 cut(s)	13, 51, 135, 168, 598, 650, 652, 1373, 3138
Cfr10I	RCCGGY	1 cut(s)	221
Cfr13I	GGNCC	3 cut(s)	214, 1598, 3245
ClaI	ATCGAT	1 cut(s)	498
CspAI	ACCGGT	1 cut(s)	221
DinI	GGCGCC	1 cut(s)	12
DraI	TTTAAA	2 cut(s)	1474, 3229
Eam1104I	CTCTTC	6 cut(s)	339, 756, 873, 1701, 2319, 3602
EarI	CTCTTC	6 cut(s)	339, 756, 873, 1701, 2319, 3602
Ecl136II	GAGCTC	3 cut(s)	30, 361, 3401
Eco130I	CCWWGG	3 cut(s)	210, 2866, 3106
Eco24I	GRGCYC	4 cut(s)	32, 304, 363, 3403
Eco47I	GGWCC	1 cut(s)	1598
Eco53kI	GAGCTC	3 cut(s)	30, 361, 3401
Eco57I	CTGAAG	9 cut(s)	65, 320, 1152, 1326, 1584, 1725, 2778, 3018, 3583
Eco88I	CYCGRG	2 cut(s)	260, 1449
EcoICRI	GAGCTC	3 cut(s)	30, 361, 3401
EcoNI	CCTNNNNNAGG	1 cut(s)	1452
EcoO109I	RGGNCCY	1 cut(s)	1598
EcoRII	CCWGG	2 cut(s)	466, 995
EcoT14I	CCWWGG	3 cut(s)	210, 2866, 3106
EcoT22I	ATGCAT	1 cut(s)	1381
EcoT38I	GRGCYC	4 cut(s)	32, 304, 363, 3403
EgeI	GGCGCC	1 cut(s)	12
EheI	GGCGCC	1 cut(s)	12
ErhI	CCWWGG	3 cut(s)	210, 2866, 3106
FalI	AAGNNNNNCTT	6 cut(s)	2040, 2072, 2688, 2720, 2928, 2960
FaqI	GGGAC	5 cut(s)	134, 266, 1860, 2295, 3159
FauI	CCCGC	2 cut(s)	291, 1837
FauNDI	CATATG	3 cut(s)	1836, 2467, 3622
FbaI	TGATCA	1 cut(s)	1243
FokI	GGATG	7 cut(s)	236, 1235, 1449, 1808, 2355, 3248, 3401
FriOI	GRGCYC	4 cut(s)	32, 304, 363, 3403
FspI	TGCGCA	3 cut(s)	597, 1372, 3137
GlaI	GCGC	9 cut(s)	12, 50, 134, 167, 597, 649, 651, 1372, 3137
GsuI	CTGGAG	1 cut(s)	102
HaeII	RGCGCY	2 cut(s)	14, 52
HaeIII	GGCC	4 cut(s)	215, 545, 1186, 3246
HapII	CCGG	6 cut(s)	192, 222, 228, 446, 2158, 2372
HhaI	GCGC	9 cut(s)	13, 51, 135, 168, 598, 650, 652, 1373, 3138
Hin1I	GRCGYC	2 cut(s)	11, 201
Hin6I	GCGC	9 cut(s)	11, 49, 133, 166, 596, 648, 650, 1371, 3136
HinP1I	GCGC	9 cut(s)	11, 49, 133, 166, 596, 648, 650, 1371, 3136
HincII	GTYRAC	1 cut(s)	3325
HindII	GTYRAC	1 cut(s)	3325
HindIII	AAGCTT	1 cut(s)	3419
HpaII	CCGG	6 cut(s)	192, 222, 228, 446, 2158, 2372
Hpy166II	GTNNAC	6 cut(s)	351, 392, 2096, 2549, 3325, 3649
Hpy8I	GTNNAC	6 cut(s)	351, 392, 2096, 2549, 3325, 3649
Hpy99I	CGWCG	4 cut(s)	203, 206, 209, 3222
HpyCH4III	ACNGT	2 cut(s)	614, 1524
HpyCH4IV	ACGT	6 cut(s)	24, 201, 279, 821, 1687, 2092
HpyF10VI	GCNNNNNNNGC	9 cut(s)	36, 283, 662, 1091, 2144, 2528, 2591, 2891, 3182
HpySE526I	ACGT	6 cut(s)	24, 201, 279, 821, 1687, 2092
Hsp92I	GRCGYC	2 cut(s)	11, 201
HspAI	GCGC	9 cut(s)	11, 49, 133, 166, 596, 648, 650, 1371, 3136
KasI	GGCGCC	1 cut(s)	10
Kpn2I	TCCGGA	2 cut(s)	191, 2371
KpnI	GGTACC	2 cut(s)	2876, 3116
Ksp22I	TGATCA	1 cut(s)	1243
LguI	GCTCTTC	1 cut(s)	339
LmnI	GCTCC	3 cut(s)	274, 430, 796
MaeII	ACGT	6 cut(s)	24, 201, 279, 821, 1687, 2092
MaeIII	GTNAC	7 cut(s)	908, 979, 1123, 2008, 2618, 3159, 3334
MfeI	CAATTG	1 cut(s)	1033
MflI	RGATCY	5 cut(s)	376, 1153, 1906, 2698, 2938
MhlI	GDGCHC	5 cut(s)	32, 279, 304, 363, 3403
Mly113I	GGCGCC	1 cut(s)	11
MlyI	GAGTC	4 cut(s)	101, 1842, 1871, 3588
MmeI	TCCRAC	2 cut(s)	1079, 1869
Mph1103I	ATGCAT	1 cut(s)	1381
MroI	TCCGGA	2 cut(s)	191, 2371
MroXI	GAANNNNTTC	6 cut(s)	223, 1532, 2409, 2770, 3010, 3517
MslI	CAYNNNNRTG	1 cut(s)	254
MspA1I	CMGCKG	4 cut(s)	601, 2522, 3173, 3193
MspI	CCGG	6 cut(s)	192, 222, 228, 446, 2158, 2372
MspR9I	CCNGG	3 cut(s)	446, 468, 997
MunI	CAATTG	1 cut(s)	1033
Mva1269I	GAATGC	2 cut(s)	2140, 3185
MvaI	CCWGG	2 cut(s)	468, 997
MvnI	CGCG	3 cut(s)	54, 650, 2001
MwoI	GCNNNNNNNGC	9 cut(s)	36, 283, 662, 1091, 2144, 2528, 2591, 2891, 3182
NarI	GGCGCC	1 cut(s)	11
NciI	CCSGG	1 cut(s)	446
NcoI	CCATGG	1 cut(s)	210
NdeI	CATATG	3 cut(s)	1836, 2467, 3622
NheI	GCTAGC	3 cut(s)	2015, 2680, 2920
NlaIV	GGNNCC	7 cut(s)	12, 226, 1599, 2044, 2183, 2874, 3114
NmeAIII	GCCGAG	1 cut(s)	2294
NmuCI	GTSAC	5 cut(s)	908, 1123, 2008, 2618, 3159
NsbI	TGCGCA	3 cut(s)	597, 1372, 3137
NsiI	ATGCAT	1 cut(s)	1381
NspI	RCATGY	2 cut(s)	773, 3350
NspV	TTCGAA	2 cut(s)	2115, 3382
PaeI	GCATGC	1 cut(s)	773
PaeR7I	CTCGAG	1 cut(s)	260
PagI	TCATGA	2 cut(s)	919, 1645
PauI	GCGCGC	1 cut(s)	648
PciI	ACATGT	1 cut(s)	3346
PciSI	GCTCTTC	1 cut(s)	339
PctI	GAATGC	2 cut(s)	2140, 3185
PdmI	GAANNNNTTC	6 cut(s)	223, 1532, 2409, 2770, 3010, 3517
PflFI	GACNNNGTC	1 cut(s)	203
PflMI	CCANNNNNTGG	1 cut(s)	373
PinAI	ACCGGT	1 cut(s)	221
PleI	GAGTC	4 cut(s)	100, 1842, 1871, 3588
PluTI	GGCGCC	1 cut(s)	14
PpsI	GAGTC	4 cut(s)	100, 1842, 1871, 3588
Ppu21I	YACGTR	1 cut(s)	25
PpuMI	RGGWCCY	1 cut(s)	1598
PscI	ACATGT	1 cut(s)	3346
PsiI	TTATAA	2 cut(s)	455, 1811
Psp124BI	GAGCTC	3 cut(s)	32, 363, 3403
Psp1406I	AACGTT	1 cut(s)	1687
Psp5II	RGGWCCY	1 cut(s)	1598
Psp6I	CCWGG	2 cut(s)	466, 995
PspGI	CCWGG	2 cut(s)	466, 995
PspN4I	GGNNCC	7 cut(s)	12, 226, 1599, 2044, 2183, 2874, 3114
PspPI	GGNCC	3 cut(s)	214, 1598, 3245
PspPPI	RGGWCCY	1 cut(s)	1598
PspXI	VCTCGAGB	1 cut(s)	260
PsuI	RGATCY	5 cut(s)	376, 1153, 1906, 2698, 2938
PsyI	GACNNNGTC	1 cut(s)	203
PteI	GCGCGC	1 cut(s)	648
PvuII	CAGCTG	3 cut(s)	601, 2522, 3193
RseI	CAYNNNNRTG	1 cut(s)	254
SacI	GAGCTC	3 cut(s)	32, 363, 3403
SapI	GCTCTTC	1 cut(s)	339
Sau96I	GGNCC	3 cut(s)	214, 1598, 3245
ScaI	AGTACT	1 cut(s)	2288
SchI	GAGTC	4 cut(s)	101, 1842, 1871, 3588
ScrFI	CCNGG	3 cut(s)	446, 468, 997
SduI	GDGCHC	5 cut(s)	32, 279, 304, 363, 3403
SfcI	CTRYAG	1 cut(s)	2580
SfoI	GGCGCC	1 cut(s)	12
Sfr274I	CTCGAG	1 cut(s)	260
SfuI	TTCGAA	2 cut(s)	2115, 3382
SinI	GGWCC	1 cut(s)	1598
SlaI	CTCGAG	1 cut(s)	260
SmiMI	CAYNNNNRTG	1 cut(s)	254
SmlI	CTYRAG	6 cut(s)	260, 344, 1484, 2057, 2351, 2394
SmoI	CTYRAG	6 cut(s)	260, 344, 1484, 2057, 2351, 2394
SpeI	ACTAGT	2 cut(s)	394, 3515
SphI	GCATGC	1 cut(s)	773
SspDI	GGCGCC	1 cut(s)	10
SspI	AATATT	1 cut(s)	1470
SstI	GAGCTC	3 cut(s)	32, 363, 3403
StyD4I	CCNGG	3 cut(s)	444, 466, 995
StyI	CCWWGG	3 cut(s)	210, 2866, 3106
TaaI	ACNGT	2 cut(s)	614, 1524
TaiI	ACGT	6 cut(s)	27, 204, 282, 824, 1690, 2095
TaqI	TCGA	7 cut(s)	90, 261, 498, 1081, 2115, 2473, 3382
TaqII	GACCGA	1 cut(s)	2019
TatI	WGTACW	5 cut(s)	1980, 2286, 2365, 2635, 3648
TauI	GCSGC	8 cut(s)	11, 16, 54, 242, 700, 776, 2022, 2206
TscAI	CASTG	6 cut(s)	1114, 1616, 1860, 2128, 2457, 2492
TseFI	GTSAC	5 cut(s)	908, 1123, 2008, 2618, 3159
Tsp45I	GTSAC	5 cut(s)	908, 1123, 2008, 2618, 3159
TspGWI	ACGGA	3 cut(s)	1211, 3206, 3493
TspRI	CASTG	6 cut(s)	1114, 1616, 1860, 2128, 2457, 2492
Tth111I	GACNNNGTC	1 cut(s)	203
Van91I	CCANNNNNTGG	1 cut(s)	373
VpaK11BI	GGWCC	1 cut(s)	1598
XagI	CCTNNNNNAGG	1 cut(s)	1452
XapI	RAATTY	2 cut(s)	933, 2331
XbaI	TCTAGA	2 cut(s)	1555, 1903
XceI	RCATGY	2 cut(s)	773, 3350
XcmI	CCANNNNNNNNNTGG	1 cut(s)	325
XhoI	CTCGAG	1 cut(s)	260
XmaJI	CCTAGG	2 cut(s)	2866, 3106
XmnI	GAANNNNTTC	6 cut(s)	223, 1532, 2409, 2770, 3010, 3517
ZraI	GACGTC	1 cut(s)	202
ZrmI	AGTACT	1 cut(s)	2288
Zsp2I	ATGCAT	1 cut(s)	1381

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.