Rmu_sc0002113.1_g000007

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002113.1
OG0013803
Physical Location & Seq
Reverse (-)
34760 .. 36421
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 990 bp
atggcttcctccgaagaccaccatccctaccgccccacatcgaaaccctccaagcctttttcccttaccaacttttccacatggaaaataaaagccaacaacgcctacaccttgccactcatcctcttcctgtgcatcttctcttactacttcggcctctggcaacacagcagcaccgccccctccaccaccaccaccaccacctccgccctcaaccaacttccgtgtaactcccccgatacaaccctaaccaaaaaccctaaaaaagccctagatttcgccacccaccacaaccctaacttcgccatcaacatttccaaacacaacactcccaaaacctaccctccctgcgacgtcaagtacagtgagtacaccccatgcgaggacgcgaagcgatccttgaaattcgagagggatagactgatatacagggagaggcactgtccagctcaggatgagcttctcaagtgccgtgttccggcacctcacggctaccggaacacctttacttggcccaaaagtagagatctggcgtggtacgccaatgttccgcacaaggagttgacagtggagaaggcggtgcagaattggattatataccaaggcgaccggtttaagttccccggcggtggaaccatgtttccgaatggcgccgatgcctacattgatgatattgggaagttgatcaatctcaaggacgggtctattcggaccgccattgatacaggctgtggggttgctagttggggagcttatctattgtcccgggatattctaccaatgtctttcgcgccaagagacacacatgaagcccaagtccaattcgctctcgagcgaggagtacccgctctcatcggcgtcattgcgtcgaaaagacttccttacccttctagagcctttgacatggctcattgctctcgttgcctcattccttggggccaatacggtactttcaccttcaaatatttatttttcggaaacgaattttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

329

Amino Acids

37.34

Weight (kDa)

9.09

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 123 - 318 4.4e-95 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
AccB1I GGYRCC 2 cut(s) 481, 650
AccBSI CCGCTC 1 cut(s) 848
AccII CGCG 2 cut(s) 389, 791
AciI CCGC 8 cut(s) 31, 177, 207, 551, 578, 627, 714, 846
AclWI GGATC 1 cut(s) 390
AcsI RAATTY 2 cut(s) 404, 983
AcyI GRCGYC 3 cut(s) 354, 651, 858
AfaI GTAC 5 cut(s) 362, 371, 539, 843, 949
AfiI CCNNNNNNNGG 4 cut(s) 382, 478, 510, 629
AgeI ACCGGT 1 cut(s) 609
AgsI TTSAA 2 cut(s) 403, 961
AloI GAACNNNNNNTCC 2 cut(s) 625, 657
AluBI AGCT 3 cut(s) 449, 460, 752
AluI AGCT 3 cut(s) 449, 460, 752
Alw26I GTCTC 1 cut(s) 792
AlwI GGATC 1 cut(s) 390
Ama87I CYCGRG 2 cut(s) 765, 830
AoxI GGCC 3 cut(s) 154, 512, 937
ApeKI GCWGC 1 cut(s) 171
ApoI RAATTY 2 cut(s) 404, 983
AsiGI ACCGGT 1 cut(s) 609
AspLEI GCGC 2 cut(s) 653, 793
AspS9I GGNCC 3 cut(s) 513, 711, 937
AsuC2I CCSGG 3 cut(s) 624, 766, 767
AsuHPI GGTGA 1 cut(s) 946
AvaI CYCGRG 2 cut(s) 765, 830
AvaII GGWCC 1 cut(s) 711
BanI GGYRCC 2 cut(s) 481, 650
BbsI GAAGAC 1 cut(s) 21
BbvI GCAGC 1 cut(s) 183
BccI CCATC 2 cut(s) 30, 314
BceAI ACGGC 2 cut(s) 456, 505
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 3 cut(s) 624, 766, 767
BcoDI GTCTC 1 cut(s) 792
BfaI CTAG 3 cut(s) 272, 741, 891
BfoI RGCGCY 1 cut(s) 654
BglII AGATCT 1 cut(s) 526
BisI GCNGC 1 cut(s) 172
BlsI GCNGC 1 cut(s) 173
Bme1390I CCNGG 3 cut(s) 624, 766, 767
Bme18I GGWCC 1 cut(s) 711
BmeT110I CYCGRG 2 cut(s) 765, 830
BmgT120I GGNCC 3 cut(s) 513, 711, 937
BmiI GGNNCC 4 cut(s) 483, 634, 652, 938
BmrFI CCNGG 3 cut(s) 624, 766, 767
BmsI GCATC 2 cut(s) 144, 646
BpiI GAAGAC 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 450
BpuEI CTTGAG 2 cut(s) 449, 677
BpuMI CCSGG 3 cut(s) 624, 766, 767
BsaHI GRCGYC 3 cut(s) 354, 651, 858
BsaJI CCNNGG 4 cut(s) 601, 622, 765, 932
BsaWI WCCGGW 2 cut(s) 495, 609
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 4 cut(s) 382, 478, 510, 629
Bse118I RCCGGY 1 cut(s) 609
Bse3DI GCAATG 2 cut(s) 861, 910
BseDI CCNNGG 4 cut(s) 601, 622, 765, 932
BseGI GGATG 3 cut(s) 22, 120, 460
BseLI CCNNNNNNNGG 4 cut(s) 382, 478, 510, 629
BseMI GCAATG 2 cut(s) 861, 910
BseMII CTCAG 1 cut(s) 464
BseRI GAGGAG 1 cut(s) 852
BseXI GCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 2 cut(s) 389, 791
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 3 cut(s) 156, 514, 939
BshNI GGYRCC 2 cut(s) 481, 650
BshTI ACCGGT 1 cut(s) 609
BsiEI CGRYCG 1 cut(s) 610
BsiHKCI CYCGRG 2 cut(s) 765, 830
BsiSI CCGG 5 cut(s) 479, 496, 610, 624, 766
BslFI GGGAC 1 cut(s) 748
BslI CCNNNNNNNGG 4 cut(s) 382, 478, 510, 629
BsmAI GTCTC 1 cut(s) 792
BsmFI GGGAC 1 cut(s) 748
BsnI GGCC 3 cut(s) 156, 514, 939
BsoBI CYCGRG 2 cut(s) 765, 830
Bsp143I GATC 3 cut(s) 395, 526, 684
BspACI CCGC 8 cut(s) 31, 177, 207, 551, 578, 627, 714, 846
BspANI GGCC 3 cut(s) 156, 514, 939
BspCNI CTCAG 1 cut(s) 463
BspFNI CGCG 2 cut(s) 389, 791
BspLI GGNNCC 4 cut(s) 483, 634, 652, 938
BspPI GGATC 1 cut(s) 390
BspT107I GGYRCC 2 cut(s) 481, 650
BsrBI CCGCTC 1 cut(s) 848
BsrDI GCAATG 2 cut(s) 861, 910
BsrFI RCCGGY 1 cut(s) 609
BssAI RCCGGY 1 cut(s) 609
BssECI CCNNGG 4 cut(s) 601, 622, 765, 932
BssMI GATC 3 cut(s) 395, 526, 684
BssNI GRCGYC 3 cut(s) 354, 651, 858
BssT1I CCWWGG 2 cut(s) 601, 932
Bst4CI ACNGT 4 cut(s) 365, 443, 568, 947
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 3 cut(s) 354, 651, 858
BstDEI CTNAG 1 cut(s) 450
BstF5I GGATG 3 cut(s) 22, 120, 460
BstFNI CGCG 2 cut(s) 389, 791
BstH2I RGCGCY 1 cut(s) 654
BstHHI GCGC 2 cut(s) 653, 793
BstKTI GATC 3 cut(s) 398, 529, 687
BstMAI GTCTC 1 cut(s) 792
BstMBI GATC 3 cut(s) 395, 526, 684
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 3 cut(s) 101, 539, 921
BstSCI CCNGG 3 cut(s) 622, 764, 765
BstUI CGCG 2 cut(s) 389, 791
BstV1I GCAGC 1 cut(s) 183
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 3 cut(s) 156, 514, 939
BtsCI GGATG 3 cut(s) 22, 120, 460
BtsIMutI CAGTG 3 cut(s) 370, 439, 573
CfoI GCGC 2 cut(s) 653, 793
Cfr10I RCCGGY 1 cut(s) 609
Cfr13I GGNCC 3 cut(s) 513, 711, 937
Cfr9I CCCGGG 1 cut(s) 765
CpoI CGGWCCG 1 cut(s) 711
CseI GACGC 3 cut(s) 395, 847, 855
Csp6I GTAC 5 cut(s) 361, 370, 538, 842, 948
CspAI ACCGGT 1 cut(s) 609
CspI CGGWCCG 1 cut(s) 711
CviAII CATG 5 cut(s) 81, 378, 637, 806, 904
CviQI GTAC 5 cut(s) 361, 370, 538, 842, 948
DdeI CTNAG 1 cut(s) 450
DinI GGCGCC 1 cut(s) 652
DpnI GATC 3 cut(s) 397, 528, 686
DpnII GATC 3 cut(s) 395, 526, 684
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 196
Eco130I CCWWGG 2 cut(s) 601, 932
Eco47I GGWCC 1 cut(s) 711
Eco88I CYCGRG 2 cut(s) 765, 830
EcoT14I CCWWGG 2 cut(s) 601, 932
EgeI GGCGCC 1 cut(s) 652
EheI GGCGCC 1 cut(s) 652
ErhI CCWWGG 2 cut(s) 601, 932
FaeI CATG 5 cut(s) 84, 381, 640, 809, 907
FaiI YATR 8 cut(s) 82, 379, 427, 596, 598, 638, 807, 905
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 865, 897
FaqI GGGAC 1 cut(s) 748
FatI CATG 5 cut(s) 80, 377, 636, 805, 903
FauI CCCGC 1 cut(s) 853
FbaI TGATCA 1 cut(s) 684
Fnu4HI GCNGC 1 cut(s) 172
FokI GGATG 3 cut(s) 9, 107, 467
Fsp4HI GCNGC 1 cut(s) 172
FspBI CTAG 3 cut(s) 272, 741, 891
GlaI GCGC 2 cut(s) 652, 792
GluI GCNGC 1 cut(s) 172
HaeII RGCGCY 1 cut(s) 654
HaeIII GGCC 3 cut(s) 156, 514, 939
HapII CCGG 5 cut(s) 479, 496, 610, 624, 766
HgaI GACGC 3 cut(s) 395, 847, 855
HhaI GCGC 2 cut(s) 653, 793
Hin1I GRCGYC 3 cut(s) 354, 651, 858
Hin1II CATG 5 cut(s) 84, 381, 640, 809, 907
Hin6I GCGC 2 cut(s) 651, 791
HinP1I GCGC 2 cut(s) 651, 791
HincII GTYRAC 1 cut(s) 564
HindII GTYRAC 1 cut(s) 564
HpaII CCGG 5 cut(s) 479, 496, 610, 624, 766
HphI GGTGA 1 cut(s) 946
Hpy166II GTNNAC 2 cut(s) 372, 564
Hpy188I TCNGA 4 cut(s) 13, 645, 711, 977
Hpy188III TCNNGA 4 cut(s) 409, 452, 830, 891
Hpy8I GTNNAC 2 cut(s) 372, 564
Hpy99I CGWCG 2 cut(s) 356, 871
HpyAV CCTTC 3 cut(s) 568, 897, 967
HpyCH4III ACNGT 4 cut(s) 365, 443, 568, 947
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 2 cut(s) 135, 583
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 539, 921
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 1 cut(s) 354
Hsp92I GRCGYC 3 cut(s) 354, 651, 858
Hsp92II CATG 5 cut(s) 84, 381, 640, 809, 907
HspAI GCGC 2 cut(s) 651, 791
KasI GGCGCC 1 cut(s) 650
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 3 cut(s) 395, 526, 684
LmnI GCTCC 1 cut(s) 749
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 2 cut(s) 144, 646
MaeI CTAG 3 cut(s) 272, 741, 891
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 1 cut(s) 227
MalI GATC 3 cut(s) 397, 528, 686
MbiI CCGCTC 1 cut(s) 848
MboI GATC 3 cut(s) 395, 526, 684
MboII GAAGA 3 cut(s) 26, 118, 130
MflI RGATCY 1 cut(s) 526
MluCI AATT 4 cut(s) 404, 586, 821, 983
Mly113I GGCGCC 1 cut(s) 651
MseI TTAA 1 cut(s) 615
MspI CCGG 5 cut(s) 479, 496, 610, 624, 766
MspR9I CCNGG 3 cut(s) 624, 766, 767
MvnI CGCG 2 cut(s) 389, 791
MwoI GCNNNNNNNGC 3 cut(s) 101, 539, 921
NarI GGCGCC 1 cut(s) 651
NciI CCSGG 3 cut(s) 624, 766, 767
NdeII GATC 3 cut(s) 395, 526, 684
NlaIII CATG 5 cut(s) 84, 381, 640, 809, 907
NlaIV GGNNCC 4 cut(s) 483, 634, 652, 938
PaeR7I CTCGAG 1 cut(s) 830
PinAI ACCGGT 1 cut(s) 609
PkrI GCNGC 1 cut(s) 173
PluTI GGCGCC 1 cut(s) 654
PspN4I GGNNCC 4 cut(s) 483, 634, 652, 938
PspPI GGNCC 3 cut(s) 513, 711, 937
PsuI RGATCY 1 cut(s) 526
RsaI GTAC 5 cut(s) 362, 371, 539, 843, 949
RsaNI GTAC 5 cut(s) 361, 370, 538, 842, 948
Rsr2I CGGWCCG 1 cut(s) 711
RsrII CGGWCCG 1 cut(s) 711
SaqAI TTAA 1 cut(s) 615
SatI GCNGC 1 cut(s) 172
Sau3AI GATC 3 cut(s) 395, 526, 684
Sau96I GGNCC 3 cut(s) 513, 711, 937
ScrFI CCNGG 3 cut(s) 624, 766, 767
SfaNI GCATC 2 cut(s) 144, 646
SfoI GGCGCC 1 cut(s) 652
Sfr274I CTCGAG 1 cut(s) 830
SinI GGWCC 1 cut(s) 711
SlaI CTCGAG 1 cut(s) 830
SmaI CCCGGG 1 cut(s) 767
SmlI CTYRAG 3 cut(s) 464, 692, 830
SmoI CTYRAG 3 cut(s) 464, 692, 830
Sse9I AATT 4 cut(s) 404, 586, 821, 983
SsiI CCGC 8 cut(s) 31, 177, 207, 551, 578, 627, 714, 846
SspDI GGCGCC 1 cut(s) 650
SspI AATATT 1 cut(s) 965
SspMI CTAG 3 cut(s) 272, 741, 891
StyD4I CCNGG 3 cut(s) 622, 764, 765
StyI CCWWGG 2 cut(s) 601, 932
TaaI ACNGT 4 cut(s) 365, 443, 568, 947
TaiI ACGT 1 cut(s) 357
TaqI TCGA 4 cut(s) 41, 408, 831, 869
TasI AATT 4 cut(s) 404, 586, 821, 983
TatI WGTACW 2 cut(s) 360, 369
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TscAI CASTG 3 cut(s) 370, 446, 573
TseI GCWGC 1 cut(s) 171
TspDTI ATGAA 1 cut(s) 822
TspGWI ACGGA 1 cut(s) 213
TspMI CCCGGG 1 cut(s) 765
TspRI CASTG 3 cut(s) 370, 446, 573
VpaK11BI GGWCC 1 cut(s) 711
XapI RAATTY 2 cut(s) 404, 983
XbaI TCTAGA 1 cut(s) 890
XhoI CTCGAG 1 cut(s) 830
XmaI CCCGGG 1 cut(s) 765
XspI CTAG 3 cut(s) 272, 741, 891
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.