Rmu_sc0002288.1_g000016
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002288.1
OG0016140
Physical Location & Seq
Reverse (-)
57465 .. 63079
5615 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3171 bp
atgaacagtttcactctcactcttgtctactggttctttgttttggcgggttccgcccactcggccaccgagtcggagctccgatccctctacgagttcaagaaagggatccaaattgacccgctccggaaagtcctcgacacgtggaccgacatctcactcaaccaagctcagccttgccctccctggaccggcgtttactgcgacgacggcggcaatgtcgtcgccgtggcactcgacggcctcgccctcggcggcgagcttaagttgaataccctcactggcctcactgcattgcaaaacctcagtcttgccaacaatgacttcacgggtcgggtcccgcccgctctgggcgcaatgtcctctctccagcacttggatctgtccgcgaaccggttctacgggccgatcccgtcccggattaacgacttgtggggcttgaattatctcaacttgtctcggaatctcttcaaaggtgggttcccgggcccggcgtcgaatctcaatcagctcaaggtgttcgatgtgcattacaatcagctgtggggggacatttcggatttgttgcaggagtttcataacgtggagtacgttgatttgagctacaatgagtttttcggagggattgagctgggttctgagaacatttcgagcttatccaacactgtgcggcacttgaacttcagccacaataatctgactggccggttctttaaggctgattcaatcgggttgtttaggaacttgcaagttttggacttgggtgggaatcagatcactggggagctcccttcattcgggtcgttgccgaatttgcgggttttgaggctggcgaataatcagttctttggggaaataccggaggtgttgctacagagttctattccaatggaggagttggatcttagtggaaatgcttttacaggttccatcactggaattaactctacaactttgaaagtcttgaatctgtcatcaaatggtatgtcgggaacattgcagaatgtggatatgaggagttgtgttgtggtagatttgagcagaaataagatttcaggagatatatcaatgttggagaatatgggtactgctttagaggttctcgatttgagttcaaacaatttttctggaagcttaacaaacttgaatatgcagttcgagagattaactacacttagtctgagagataatttattagcagggtctttgccttcaatactgaaggcctgccccagactctctacagttgacttgagtcgaaatgatttcagtgggtccattcctggtagtttcttctcttcactgacattgacaaggttgaatctttcacgaaatcacttaaatggaccaattcctctccaaggcggacatgtaagcgaattcttagctcttcctccagatttgccaattgagaatgtcgatctgtccaataattctttatcgggttcccttcctcgggacattggtaacatggtggaactcaaattgctaaaccttgcaaagaatgagttttctggagaactacctagtgaattgagtaaacttgctaagttgaagtaccttgatttatcaggtaacaaattcaagggcgcaattcctgagcagcttccatccagcttaattgtatttaatgtctcctataatgatctgtctggttctgttccggacaatttaaaaagcttccccatgacttcatttcgtcctggaaatgagttgctaaacttaccaaatgatgatcgggctagatcttcagttccaggtcgtattccagaacaaggaaaaagtcatacttcaaaggctcatattaggatagcaatcattgttgcttcagttggagttactttgatgatagtttttgttttgttggtttatcatcagacacaccataaaggatttcatggaagaaatggatttggcggcgaaaatacagggacagatgttaagattggcagattcacacgtccttcatttttgaatttccaaacgaatgttcagcctccaccaacttcattgagcttttcaaatgatcacttgctaacttcacaatccaggtcattgtcagggcaggcagagtttgtaccagaaattgggaagcctgttttacctggggaagcagcaacgagttctacacctatgaatcttcttgataaccagcctacaacttctggaaggaagtcctcccctggttctccactatcttcctcacctcgttttatagaggcatatgagcaacctgtaatactggatgtgtactcaccagatcggttggctggagaactgttcttcctagatgcttcattgcaatttacagctgaggaattatctcgagctcctgcagaagttctgggcagaagcagccatggaactctgtacaaagctactctagatagtgggcacatgttgacggtgaaatggttacgggtgggactggtcaagcataagaaagattttgccaaggaagtcaaaaggattggctctataaggcatccaaacattgttccattgcaggcatactattgggggcctagggaacaagagaggcttcttttagcagactatgttcagggagatagcttggccctacatctttatgagagcacgcctcggaggtactccccgttatcattcaaccagaggctaaaagtagctgtggaggttgctcgatgtttgctatacctacatgatagaggcctgccccatggaaacctaaagccaactaatgtaatcctggcaggtcctgagtaccatcctcggcttactgattacagtcttcaccgtctaatgacaccagctggcattgcggagcagtttttaaatatgggagctcttggataccgtgctccagaacttgctactgcagcaaaacctgtcccttctttcaaagctgacgtttatgcctttggagtgattttgatggaaatgttaaccagaagaagtgctggtgacattatatcgggtcagtcaggggcggttgaccttacagactgggttcggctttgtgatcaagaaggacgaggaatggattgcatcgacagagacattgctggtggagaagaaccctcaaaagcaatggatgaattgcttgcaatatcactgaggtgtattctccctgtaaatgagaggcctaacatcagacaagtatttgacaacctctgttctatatccctttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1056

Amino Acids

115.68

Weight (kDa)

6.09

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 69 9.5e-06 Leucine rich repeat N-terminal domain
LRR_4 PF12799 248 - 282 1.4e-08 Leucine Rich repeats (2 copies)
LRR_8 PF13855 471 - 528 5.1e-06 Leucine rich repeat
Pkinase PF00069 780 - 982 1.6e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 780 - 977 2.4e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10020 AT5G10020
fragaria_vesca FvH4_1g22060
malus_domestica MD01G1018200.v1.1
pyrus_communis pycom01g04790
rosa_chinensis RchiOBHm_Chr2g0116131
rosa_laevigata RLG00000018242
rosa_multiflora Rmu_sc0002288.1_g000016
rosa_roxburghii Rroxscaffold_2G00127470
rosa_rugosa Rorug02G0203700
rosa_samantha Rh2AG260100 Rh2BG271100 Rh2CG266400 Rh2DG268300
rosa_wichuraiana Rw2G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2723
AccB7I CCANNNNNTGG 2 cut(s) 376, 2087
AccBSI CCGCTC 2 cut(s) 124, 347
AccI GTMKAC 1 cut(s) 27
AccII CGCG 1 cut(s) 389
AccIII TCCGGA 2 cut(s) 126, 1663
AclWI GGATC 6 cut(s) 78, 103, 116, 387, 403, 909
AcoI YGGCCR 2 cut(s) 63, 703
AcsI RAATTY 4 cut(s) 811, 1379, 1580, 1975
AcuI CTGAAG 4 cut(s) 667, 1241, 1734, 1812
AcvI CACGTG 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 494
AfaI GTAC 8 cut(s) 590, 1087, 1559, 2079, 2252, 2372, 2612, 2744
AflII CTTAAG 1 cut(s) 263
AflIII ACRYGT 4 cut(s) 141, 1369, 1958, 2397
AgeI ACCGGT 1 cut(s) 393
AjiI CACGTC 1 cut(s) 1961
AjnI CCWGG 8 cut(s) 185, 1282, 1702, 1756, 2048, 2104, 2182, 2727
AjuI GAANNNNNNNTTGG 4 cut(s) 308, 340, 1974, 2006
AleI CACNNNNGTG 1 cut(s) 3097
AloI GAACNNNNNNTCC 2 cut(s) 2268, 2300
Alw21I GWGCWC 6 cut(s) 81, 789, 2332, 2600, 2827, 2842
Alw26I GTCTC 3 cut(s) 462, 1639, 3030
AlwI GGATC 6 cut(s) 78, 103, 116, 387, 403, 909
AlwNI CAGNNNCTG 1 cut(s) 2738
Ama87I CYCGRG 3 cut(s) 484, 1455, 2325
Aor13HI TCCGGA 2 cut(s) 126, 1663
ApaI GGGCCC 1 cut(s) 491
ApeKI GCWGC 4 cut(s) 1603, 2114, 2355, 2858
ApoI RAATTY 4 cut(s) 811, 1379, 1580, 1975
ArsI GACNNNNNNTTYG 4 cut(s) 292, 324, 971, 1003
AsiGI ACCGGT 1 cut(s) 393
Asp700I GAANNNNTTC 3 cut(s) 8, 395, 467
AspA2I CCTAGG 1 cut(s) 2525
AspLEI GCGC 2 cut(s) 356, 1592
AsuC2I CCSGG 4 cut(s) 418, 485, 486, 491
AsuHPI GGTGA 5 cut(s) 2196, 2247, 2419, 2765, 2954
AvaI CYCGRG 3 cut(s) 484, 1455, 2325
AvaII GGWCC 6 cut(s) 147, 189, 337, 1275, 1346, 2735
AvrII CCTAGG 1 cut(s) 2525
BaeGI GKGCMC 2 cut(s) 491, 2397
BamHI GGATCC 1 cut(s) 108
BanII GRGCYC 5 cut(s) 81, 491, 789, 2332, 2827
BbrPI CACGTG 1 cut(s) 144
BbsI GAAGAC 1 cut(s) 2762
Bbv12I GWGCWC 6 cut(s) 81, 789, 2332, 2600, 2827, 2842
BbvCI CCTCAGC 1 cut(s) 2313
BbvI GCAGC 4 cut(s) 1615, 2126, 2367, 2870
BccI CCATC 4 cut(s) 938, 1618, 2754, 2908
BceAI ACGGC 3 cut(s) 212, 226, 256
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 8 cut(s) 187, 1284, 1704, 1758, 2050, 2106, 2184, 2729
BciVI GTATCC 1 cut(s) 2825
BclI TGATCA 2 cut(s) 2026, 3001
BcnI CCSGG 4 cut(s) 418, 485, 486, 491
BcoDI GTCTC 3 cut(s) 462, 1639, 3030
BfaI CTAG 5 cut(s) 1527, 1743, 2288, 2384, 2526
BfmI CTRYAG 4 cut(s) 872, 1242, 2334, 2856
BfrI CTTAAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 2723
BfuI GTATCC 1 cut(s) 2825
BglI GCCNNNNNGGC 1 cut(s) 62
BglII AGATCT 1 cut(s) 1745
BisI GCNGC 8 cut(s) 214, 256, 671, 1604, 1918, 2115, 2356, 2859
BlnI CCTAGG 1 cut(s) 2525
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 8 cut(s) 215, 257, 672, 1605, 1919, 2116, 2357, 2860
Bme18I GGWCC 6 cut(s) 147, 189, 337, 1275, 1346, 2735
BmeT110I CYCGRG 3 cut(s) 484, 1455, 2325
BmgBI CACGTC 1 cut(s) 1961
BmrI ACTGGG 2 cut(s) 789, 2995
BmsI GCATC 3 cut(s) 2281, 2494, 3036
BmuI ACTGGG 2 cut(s) 789, 2995
BoxI GACNNNNGTC 1 cut(s) 1254
BpiI GAAGAC 1 cut(s) 2762
BplI GAGNNNNNCTC 2 cut(s) 2587, 2619
BpmI CTGGAG 5 cut(s) 353, 1380, 1536, 2292, 2826
Bpu10I CCTNAGC 2 cut(s) 1599, 2313
Bpu1102I GCTNAGC 1 cut(s) 171
BpuEI CTTGAG 2 cut(s) 497, 1273
BpuMI CCSGG 4 cut(s) 418, 485, 486, 491
BsaAI YACGTR 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 1814
BsaHI GRCGYC 1 cut(s) 494
BsaWI WCCGGW 4 cut(s) 126, 393, 859, 1663
Bse118I RCCGGY 3 cut(s) 191, 393, 705
Bse1I ACTGG 8 cut(s) 35, 286, 706, 784, 940, 2247, 2433, 2990
Bse3DI GCAATG 9 cut(s) 223, 293, 363, 995, 2297, 2501, 2796, 3039, 3075
Bse8I GATNNNNATC 1 cut(s) 1814
BseAI TCCGGA 2 cut(s) 126, 1663
BseBI CCWGG 8 cut(s) 187, 1284, 1704, 1758, 2050, 2106, 2184, 2729
BseGI GGATG 5 cut(s) 1610, 2251, 2485, 2746, 3079
BseJI GATNNNNATC 1 cut(s) 1814
BseMI GCAATG 9 cut(s) 223, 293, 363, 995, 2297, 2501, 2796, 3039, 3075
BseMII CTCAG 8 cut(s) 185, 319, 630, 1172, 1590, 2304, 2730, 3086
BseNI ACTGG 8 cut(s) 35, 286, 706, 784, 940, 2247, 2433, 2990
BseRI GAGGAG 2 cut(s) 908, 1030
BseSI GKGCMC 2 cut(s) 491, 2397
BseXI GCAGC 4 cut(s) 1615, 2126, 2367, 2870
BseYI CCCAGC 1 cut(s) 631
Bsh1236I CGCG 1 cut(s) 389
BshTI ACCGGT 1 cut(s) 393
BsiHKAI GWGCWC 6 cut(s) 81, 789, 2332, 2600, 2827, 2842
BsiHKCI CYCGRG 3 cut(s) 484, 1455, 2325
BsiSI CCGG 9 cut(s) 127, 192, 394, 418, 485, 491, 706, 860, 1664
BslFI GGGAC 7 cut(s) 323, 400, 563, 1472, 1945, 2439, 2855
BsmAI GTCTC 3 cut(s) 462, 1639, 3030
BsmFI GGGAC 7 cut(s) 323, 400, 563, 1472, 1945, 2439, 2855
BsoBI CYCGRG 3 cut(s) 484, 1455, 2325
Bsp120I GGGCCC 1 cut(s) 487
Bsp1286I GDGCHC 8 cut(s) 81, 491, 789, 2332, 2397, 2600, 2827, 2842
Bsp13I TCCGGA 2 cut(s) 126, 1663
Bsp1407I TGTACA 1 cut(s) 2370
Bsp1720I GCTNAGC 1 cut(s) 171
Bsp19I CCATGG 2 cut(s) 2359, 2698
BspCNI CTCAG 8 cut(s) 184, 318, 631, 1173, 1591, 2305, 2731, 3087
BspEI TCCGGA 2 cut(s) 126, 1663
BspFNI CGCG 1 cut(s) 389
BspMAI CTGCAG 2 cut(s) 2338, 2860
BspMI ACCTGC 1 cut(s) 2723
BspPI GGATC 6 cut(s) 78, 103, 116, 387, 403, 909
BspQI GCTCTTC 1 cut(s) 1395
BspTI CTTAAG 1 cut(s) 263
BsrBI CCGCTC 2 cut(s) 124, 347
BsrDI GCAATG 9 cut(s) 223, 293, 363, 995, 2297, 2501, 2796, 3039, 3075
BsrFI RCCGGY 3 cut(s) 191, 393, 705
BsrGI TGTACA 1 cut(s) 2370
BsrI ACTGG 8 cut(s) 35, 286, 706, 784, 940, 2247, 2433, 2990
BssAI RCCGGY 3 cut(s) 191, 393, 705
BssNI GRCGYC 1 cut(s) 494
BssT1I CCWWGG 5 cut(s) 1360, 2359, 2454, 2525, 2698
Bst2UI CCWGG 8 cut(s) 187, 1284, 1704, 1758, 2050, 2106, 2184, 2729
Bst4CI ACNGT 8 cut(s) 8, 667, 1246, 2280, 2407, 2768, 2777, 2837
Bst6I CTCTTC 3 cut(s) 473, 1303, 1395
BstACI GRCGYC 1 cut(s) 494
BstAFI CTTAAG 1 cut(s) 263
BstAUI TGTACA 1 cut(s) 2370
BstBAI YACGTR 1 cut(s) 144
BstDSI CCRYGG 3 cut(s) 228, 2359, 2698
BstENI CCTNNNNNAGG 1 cut(s) 1359
BstF5I GGATG 5 cut(s) 1610, 2251, 2485, 2746, 3079
BstFNI CGCG 1 cut(s) 389
BstHHI GCGC 2 cut(s) 356, 1592
BstMAI GTCTC 3 cut(s) 462, 1639, 3030
BstMWI GCNNNNNNNGC 9 cut(s) 53, 62, 201, 210, 353, 814, 2355, 2798, 2858
BstNI CCWGG 8 cut(s) 187, 1284, 1704, 1758, 2050, 2106, 2184, 2729
BstNSI RCATGY 2 cut(s) 1373, 2401
BstPAI GACNNNNGTC 1 cut(s) 1254
BstSFI CTRYAG 4 cut(s) 872, 1242, 2334, 2856
BstSLI GKGCMC 2 cut(s) 491, 2397
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 4 cut(s) 1615, 2126, 2367, 2870
BstV2I GAAGAC 1 cut(s) 2762
BstX2I RGATCY 4 cut(s) 108, 379, 901, 1745
BstYI RGATCY 4 cut(s) 108, 379, 901, 1745
BsuI GTATCC 1 cut(s) 2825
BtgI CCRYGG 3 cut(s) 228, 2359, 2698
BtrI CACGTC 1 cut(s) 1961
BtsCI GGATG 5 cut(s) 1610, 2251, 2485, 2746, 3079
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 8 cut(s) 279, 288, 663, 777, 933, 1276, 1301, 3092
BveI ACCTGC 1 cut(s) 2723
CaiI CAGNNNCTG 1 cut(s) 2738
CfoI GCGC 2 cut(s) 356, 1592
Cfr10I RCCGGY 3 cut(s) 191, 393, 705
Cfr9I CCCGGG 1 cut(s) 484
CseI GACGC 1 cut(s) 483
Csp6I GTAC 8 cut(s) 589, 1086, 1558, 2078, 2251, 2371, 2611, 2743
CspAI ACCGGT 1 cut(s) 393
CviAII CATG 8 cut(s) 1370, 1471, 1687, 1898, 2360, 2398, 2681, 2699
CviQI GTAC 8 cut(s) 589, 1086, 1558, 2078, 2251, 2371, 2611, 2743
DraI TTTAAA 2 cut(s) 1674, 2814
EaeI YGGCCR 2 cut(s) 63, 703
Eam1104I CTCTTC 3 cut(s) 473, 1303, 1395
EarI CTCTTC 3 cut(s) 473, 1303, 1395
EciI GGCGGA 2 cut(s) 43, 1380
Ecl136II GAGCTC 4 cut(s) 79, 787, 2330, 2825
Eco130I CCWWGG 5 cut(s) 1360, 2359, 2454, 2525, 2698
Eco147I AGGCCT 3 cut(s) 1226, 2691, 3124
Eco24I GRGCYC 5 cut(s) 81, 491, 789, 2332, 2827
Eco47I GGWCC 6 cut(s) 147, 189, 337, 1275, 1346, 2735
Eco53kI GAGCTC 4 cut(s) 79, 787, 2330, 2825
Eco57I CTGAAG 4 cut(s) 667, 1241, 1734, 1812
Eco72I CACGTG 1 cut(s) 144
Eco88I CYCGRG 3 cut(s) 484, 1455, 2325
EcoICRI GAGCTC 4 cut(s) 79, 787, 2330, 2825
EcoNI CCTNNNNNAGG 1 cut(s) 1359
EcoO109I RGGNCCY 3 cut(s) 337, 2522, 2735
EcoRI GAATTC 1 cut(s) 1379
EcoRII CCWGG 8 cut(s) 185, 1282, 1702, 1756, 2048, 2104, 2182, 2727
EcoT14I CCWWGG 5 cut(s) 1360, 2359, 2454, 2525, 2698
EcoT38I GRGCYC 5 cut(s) 81, 491, 789, 2332, 2827
ErhI CCWWGG 5 cut(s) 1360, 2359, 2454, 2525, 2698
FaeI CATG 8 cut(s) 1373, 1474, 1690, 1901, 2363, 2401, 2684, 2702
FalI AAGNNNNNCTT 4 cut(s) 1774, 1806, 2526, 2558
FaqI GGGAC 7 cut(s) 323, 400, 563, 1472, 1945, 2439, 2855
FatI CATG 8 cut(s) 1369, 1470, 1686, 1897, 2359, 2397, 2680, 2698
FauI CCCGC 5 cut(s) 40, 129, 348, 352, 810
FauNDI CATATG 1 cut(s) 2224
FbaI TGATCA 2 cut(s) 2026, 3001
FblI GTMKAC 1 cut(s) 27
Fnu4HI GCNGC 8 cut(s) 214, 256, 671, 1604, 1918, 2115, 2356, 2859
FokI GGATG 5 cut(s) 1597, 2258, 2472, 2733, 3086
FriOI GRGCYC 5 cut(s) 81, 491, 789, 2332, 2827
Fsp4HI GCNGC 8 cut(s) 214, 256, 671, 1604, 1918, 2115, 2356, 2859
FspBI CTAG 5 cut(s) 1527, 1743, 2288, 2384, 2526
GlaI GCGC 2 cut(s) 355, 1591
GluI GCNGC 8 cut(s) 214, 256, 671, 1604, 1918, 2115, 2356, 2859
GsaI CCCAGC 1 cut(s) 635
GsuI CTGGAG 5 cut(s) 353, 1380, 1536, 2292, 2826
HapII CCGG 9 cut(s) 127, 192, 394, 418, 485, 491, 706, 860, 1664
HgaI GACGC 1 cut(s) 483
HhaI GCGC 2 cut(s) 356, 1592
Hin1I GRCGYC 1 cut(s) 494
Hin1II CATG 8 cut(s) 1373, 1474, 1690, 1901, 2363, 2401, 2684, 2702
Hin6I GCGC 2 cut(s) 354, 1590
HinP1I GCGC 2 cut(s) 354, 1590
HincII GTYRAC 4 cut(s) 1249, 2403, 2925, 2974
HindII GTYRAC 4 cut(s) 1249, 2403, 2925, 2974
HindIII AAGCTT 2 cut(s) 1132, 1678
HpaI GTTAAC 1 cut(s) 2925
HpaII CCGG 9 cut(s) 127, 192, 394, 418, 485, 491, 706, 860, 1664
HphI GGTGA 5 cut(s) 2196, 2247, 2419, 2765, 2954
Hpy166II GTNNAC 9 cut(s) 28, 147, 199, 1249, 1541, 2251, 2403, 2925, 2974
Hpy8I GTNNAC 9 cut(s) 28, 147, 199, 1249, 1541, 2251, 2403, 2925, 2974
Hpy99I CGWCG 5 cut(s) 209, 212, 227, 242, 499
HpyAV CCTTC 8 cut(s) 801, 1216, 1221, 1460, 1974, 2163, 2883, 3002
HpyCH4III ACNGT 8 cut(s) 8, 667, 1246, 2280, 2407, 2768, 2777, 2837
HpyCH4IV ACGT 5 cut(s) 143, 582, 591, 1960, 2889
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 62, 201, 210, 353, 814, 2355, 2798, 2858
HpySE526I ACGT 5 cut(s) 143, 582, 591, 1960, 2889
Hsp92I GRCGYC 1 cut(s) 494
Hsp92II CATG 8 cut(s) 1373, 1474, 1690, 1901, 2363, 2401, 2684, 2702
HspAI GCGC 2 cut(s) 354, 1590
KflI GGGWCCC 1 cut(s) 337
Kpn2I TCCGGA 2 cut(s) 126, 1663
Ksp22I TGATCA 2 cut(s) 2026, 3001
KspAI GTTAAC 1 cut(s) 2925
LguI GCTCTTC 1 cut(s) 1395
LmnI GCTCC 9 cut(s) 76, 84, 129, 784, 792, 2335, 2803, 2822, 2845
Lsp1109I GCAGC 4 cut(s) 1615, 2126, 2367, 2870
LweI GCATC 3 cut(s) 2281, 2494, 3036
MaeI CTAG 5 cut(s) 1527, 1743, 2288, 2384, 2526
MaeII ACGT 5 cut(s) 143, 582, 591, 1960, 2889
MaeIII GTNAC 5 cut(s) 1466, 1574, 1837, 2415, 2942
MbiI CCGCTC 2 cut(s) 124, 347
MfeI CAATTG 1 cut(s) 1407
MflI RGATCY 4 cut(s) 108, 379, 901, 1745
MhlI GDGCHC 8 cut(s) 81, 491, 789, 2332, 2397, 2600, 2827, 2842
MlyI GAGTC 3 cut(s) 80, 1230, 1264
MmeI TCCRAC 5 cut(s) 54, 684, 879, 1053, 1813
MroI TCCGGA 2 cut(s) 126, 1663
MroXI GAANNNNTTC 3 cut(s) 8, 395, 467
MslI CAYNNNNRTG 3 cut(s) 1341, 2589, 3097
MspA1I CMGCKG 3 cut(s) 541, 2312, 2792
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 9 cut(s) 127, 192, 394, 418, 485, 491, 706, 860, 1664
MunI CAATTG 1 cut(s) 1407
MvaI CCWGG 8 cut(s) 187, 1284, 1704, 1758, 2050, 2106, 2184, 2729
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 9 cut(s) 53, 62, 201, 210, 353, 814, 2355, 2798, 2858
NciI CCSGG 4 cut(s) 418, 485, 486, 491
NcoI CCATGG 2 cut(s) 2359, 2698
NdeI CATATG 1 cut(s) 2224
NlaIII CATG 8 cut(s) 1373, 1474, 1690, 1901, 2363, 2401, 2684, 2702
NmeAIII GCCGAG 3 cut(s) 41, 231, 2731
NmuCI GTSAC 1 cut(s) 2942
NspI RCATGY 2 cut(s) 1373, 2401
OliI CACNNNNGTG 1 cut(s) 3097
PaeR7I CTCGAG 1 cut(s) 2325
PceI AGGCCT 3 cut(s) 1226, 2691, 3124
PciI ACATGT 2 cut(s) 1369, 2397
PciSI GCTCTTC 1 cut(s) 1395
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PdmI GAANNNNTTC 3 cut(s) 8, 395, 467
PfeI GAWTC 8 cut(s) 463, 499, 722, 769, 967, 1321, 1953, 2137
PflMI CCANNNNNTGG 2 cut(s) 376, 2087
PfoI TCCNGGA 2 cut(s) 416, 1702
PinAI ACCGGT 1 cut(s) 393
PkrI GCNGC 8 cut(s) 215, 257, 672, 1605, 1919, 2116, 2357, 2860
PleI GAGTC 3 cut(s) 79, 1230, 1263
PmaCI CACGTG 1 cut(s) 144
PmlI CACGTG 1 cut(s) 144
PpsI GAGTC 3 cut(s) 79, 1230, 1263
Ppu21I YACGTR 1 cut(s) 144
PpuMI RGGWCCY 2 cut(s) 337, 2735
PscI ACATGT 2 cut(s) 1369, 2397
PshAI GACNNNNGTC 1 cut(s) 1254
Psp124BI GAGCTC 4 cut(s) 81, 789, 2332, 2827
Psp5II RGGWCCY 2 cut(s) 337, 2735
Psp6I CCWGG 8 cut(s) 185, 1282, 1702, 1756, 2048, 2104, 2182, 2727
PspCI CACGTG 1 cut(s) 144
PspFI CCCAGC 1 cut(s) 631
PspGI CCWGG 8 cut(s) 185, 1282, 1702, 1756, 2048, 2104, 2182, 2727
PspOMI GGGCCC 1 cut(s) 487
PspPPI RGGWCCY 2 cut(s) 337, 2735
PsrI GAACNNNNNNTAC 2 cut(s) 383, 415
PstI CTGCAG 2 cut(s) 2338, 2860
PstNI CAGNNNCTG 1 cut(s) 2738
PsuI RGATCY 4 cut(s) 108, 379, 901, 1745
PvuII CAGCTG 3 cut(s) 541, 2312, 2792
RsaI GTAC 8 cut(s) 590, 1087, 1559, 2079, 2252, 2372, 2612, 2744
RsaNI GTAC 8 cut(s) 589, 1086, 1558, 2078, 2251, 2371, 2611, 2743
RseI CAYNNNNRTG 3 cut(s) 1341, 2589, 3097
SacI GAGCTC 4 cut(s) 81, 789, 2332, 2827
SapI GCTCTTC 1 cut(s) 1395
SatI GCNGC 8 cut(s) 214, 256, 671, 1604, 1918, 2115, 2356, 2859
SchI GAGTC 3 cut(s) 80, 1230, 1264
SduI GDGCHC 8 cut(s) 81, 491, 789, 2332, 2397, 2600, 2827, 2842
SfaNI GCATC 3 cut(s) 2281, 2494, 3036
SfcI CTRYAG 4 cut(s) 872, 1242, 2334, 2856
Sfr274I CTCGAG 1 cut(s) 2325
SinI GGWCC 6 cut(s) 147, 189, 337, 1275, 1346, 2735
SlaI CTCGAG 1 cut(s) 2325
SmaI CCCGGG 1 cut(s) 486
SmiMI CAYNNNNRTG 3 cut(s) 1341, 2589, 3097
SmlI CTYRAG 4 cut(s) 263, 512, 1252, 2325
SmoI CTYRAG 4 cut(s) 263, 512, 1252, 2325
SseBI AGGCCT 3 cut(s) 1226, 2691, 3124
SspMI CTAG 5 cut(s) 1527, 1743, 2288, 2384, 2526
SstI GAGCTC 4 cut(s) 81, 789, 2332, 2827
StuI AGGCCT 3 cut(s) 1226, 2691, 3124
StyI CCWWGG 5 cut(s) 1360, 2359, 2454, 2525, 2698
TaaI ACNGT 8 cut(s) 8, 667, 1246, 2280, 2407, 2768, 2777, 2837
TaiI ACGT 5 cut(s) 146, 585, 594, 1963, 2892
TaqII GACCGA 1 cut(s) 164
TatI WGTACW 2 cut(s) 2250, 2370
TauI GCSGC 4 cut(s) 216, 258, 673, 1920
TfiI GAWTC 8 cut(s) 463, 499, 722, 769, 967, 1321, 1953, 2137
TscAI CASTG 8 cut(s) 286, 295, 670, 784, 940, 1276, 1308, 3099
TseFI GTSAC 1 cut(s) 2942
TseI GCWGC 4 cut(s) 1603, 2114, 2355, 2858
Tsp45I GTSAC 1 cut(s) 2942
TspMI CCCGGG 1 cut(s) 484
TspRI CASTG 8 cut(s) 286, 295, 670, 784, 940, 1276, 1308, 3099
Van91I CCANNNNNTGG 2 cut(s) 376, 2087
Vha464I CTTAAG 1 cut(s) 263
VpaK11BI GGWCC 6 cut(s) 147, 189, 337, 1275, 1346, 2735
XagI CCTNNNNNAGG 1 cut(s) 1359
XapI RAATTY 4 cut(s) 811, 1379, 1580, 1975
XbaI TCTAGA 1 cut(s) 2383
XceI RCATGY 2 cut(s) 1373, 2401
XhoI CTCGAG 1 cut(s) 2325
XmaI CCCGGG 1 cut(s) 484
XmaJI CCTAGG 1 cut(s) 2525
XmiI GTMKAC 1 cut(s) 27
XmnI GAANNNNTTC 3 cut(s) 8, 395, 467
XspI CTAG 5 cut(s) 1527, 1743, 2288, 2384, 2526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.