Scaffold protein for the de novo synthesis of iron- sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic 2Fe-2S and 4Fe-4S proteins
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| Species | Orthologous Gene IDs |
|---|---|
| malus_domestica | MD03G1171600.v1.1 MD13G1211600.v1.1 MD13G1211700.v1.1 MD14G1077900.v1.1 |
| rosa_multiflora | Rmu_sc0002363.1_g000001 |
| rosa_roxburghii | Rroxscaffold_4G00322320 Rroxscaffold_6G00390330 |
| rosa_samantha | Rh3DG317400 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfiI | CCNNNNNNNGG | 1 cut(s) | 36 |
| AflIII | ACRYGT | 1 cut(s) | 175 |
| AluBI | AGCT | 3 cut(s) | 76, 116, 139 |
| AluI | AGCT | 3 cut(s) | 76, 116, 139 |
| AoxI | GGCC | 2 cut(s) | 16, 168 |
| ApeKI | GCWGC | 2 cut(s) | 113, 188 |
| BbvCI | CCTCAGC | 1 cut(s) | 96 |
| BbvI | GCAGC | 2 cut(s) | 125, 175 |
| BccI | CCATC | 1 cut(s) | 113 |
| BfmI | CTRYAG | 1 cut(s) | 82 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 110 |
| BisI | GCNGC | 2 cut(s) | 114, 189 |
| BlsI | GCNGC | 2 cut(s) | 115, 190 |
| BmsI | GCATC | 1 cut(s) | 91 |
| BpmI | CTGGAG | 1 cut(s) | 51 |
| Bpu10I | CCTNAGC | 1 cut(s) | 96 |
| BpuEI | CTTGAG | 1 cut(s) | 52 |
| BsaJI | CCNNGG | 1 cut(s) | 9 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 36 |
| Bse1I | ACTGG | 1 cut(s) | 68 |
| BseDI | CCNNGG | 1 cut(s) | 9 |
| BseGI | GGATG | 1 cut(s) | 106 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 36 |
| BseMII | CTCAG | 2 cut(s) | 87, 177 |
| BseNI | ACTGG | 1 cut(s) | 68 |
| BseXI | GCAGC | 2 cut(s) | 125, 175 |
| Bsh1285I | CGRYCG | 1 cut(s) | 15 |
| BshFI | GGCC | 2 cut(s) | 18, 170 |
| BsiEI | CGRYCG | 1 cut(s) | 15 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 36 |
| BsnI | GGCC | 2 cut(s) | 18, 170 |
| BspANI | GGCC | 2 cut(s) | 18, 170 |
| BspCNI | CTCAG | 2 cut(s) | 88, 176 |
| BspMAI | CTGCAG | 1 cut(s) | 86 |
| BsrI | ACTGG | 1 cut(s) | 68 |
| BssECI | CCNNGG | 1 cut(s) | 9 |
| Bst6I | CTCTTC | 1 cut(s) | 66 |
| BstC8I | GCNNGC | 2 cut(s) | 93, 141 |
| BstDEI | CTNAG | 2 cut(s) | 96, 163 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 34 |
| BstF5I | GGATG | 1 cut(s) | 106 |
| BstMCI | CGRYCG | 1 cut(s) | 15 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 101, 110, 194 |
| BstNSI | RCATGY | 1 cut(s) | 179 |
| BstSFI | CTRYAG | 1 cut(s) | 82 |
| BstV1I | GCAGC | 2 cut(s) | 125, 175 |
| BsuRI | GGCC | 2 cut(s) | 18, 170 |
| BtsCI | GGATG | 1 cut(s) | 106 |
| BtsI | GCAGTG | 1 cut(s) | 79 |
| BtsIMutI | CAGTG | 1 cut(s) | 79 |
| Cac8I | GCNNGC | 2 cut(s) | 93, 141 |
| CviAII | CATG | 1 cut(s) | 176 |
| CviJI | RGCY | 9 cut(s) | 8, 18, 76, 116, 139, 143, 159, 170, 188 |
| CviKI_1 | RGCY | 9 cut(s) | 8, 18, 76, 116, 139, 143, 159, 170, 188 |
| DdeI | CTNAG | 2 cut(s) | 96, 163 |
| Eam1104I | CTCTTC | 1 cut(s) | 66 |
| EarI | CTCTTC | 1 cut(s) | 66 |
| Eco147I | AGGCCT | 1 cut(s) | 170 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 34 |
| FaeI | CATG | 1 cut(s) | 179 |
| FaiI | YATR | 2 cut(s) | 89, 177 |
| FatI | CATG | 1 cut(s) | 175 |
| Fnu4HI | GCNGC | 2 cut(s) | 114, 189 |
| FokI | GGATG | 1 cut(s) | 113 |
| Fsp4HI | GCNGC | 2 cut(s) | 114, 189 |
| GluI | GCNGC | 2 cut(s) | 114, 189 |
| GsuI | CTGGAG | 1 cut(s) | 51 |
| HaeIII | GGCC | 2 cut(s) | 18, 170 |
| Hin1II | CATG | 1 cut(s) | 179 |
| Hpy188I | TCNGA | 1 cut(s) | 166 |
| Hpy188III | TCNNGA | 1 cut(s) | 25 |
| HpyAV | CCTTC | 2 cut(s) | 30, 178 |
| HpyCH4V | TGCA | 1 cut(s) | 84 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 101, 110, 194 |
| HpyF3I | CTNAG | 2 cut(s) | 96, 163 |
| Hsp92II | CATG | 1 cut(s) | 179 |
| LpnPI | CCDG | 2 cut(s) | 81, 125 |
| Lsp1109I | GCAGC | 2 cut(s) | 125, 175 |
| LweI | GCATC | 1 cut(s) | 91 |
| MboII | GAAGA | 1 cut(s) | 53 |
| MluCI | AATT | 1 cut(s) | 42 |
| MnlI | CCTC | 6 cut(s) | 19, 29, 75, 91, 160, 181 |
| MseI | TTAA | 1 cut(s) | 120 |
| MspA1I | CMGCKG | 1 cut(s) | 116 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 101, 110, 194 |
| NlaIII | CATG | 1 cut(s) | 179 |
| NspI | RCATGY | 1 cut(s) | 179 |
| PceI | AGGCCT | 1 cut(s) | 170 |
| PciI | ACATGT | 1 cut(s) | 175 |
| PkrI | GCNGC | 2 cut(s) | 115, 190 |
| PscI | ACATGT | 1 cut(s) | 175 |
| PstI | CTGCAG | 1 cut(s) | 86 |
| PvuII | CAGCTG | 1 cut(s) | 116 |
| SaqAI | TTAA | 1 cut(s) | 120 |
| SatI | GCNGC | 2 cut(s) | 114, 189 |
| SetI | ASST | 4 cut(s) | 32, 78, 118, 141 |
| SfaNI | GCATC | 1 cut(s) | 91 |
| SfcI | CTRYAG | 1 cut(s) | 82 |
| SmlI | CTYRAG | 1 cut(s) | 31 |
| SmoI | CTYRAG | 1 cut(s) | 31 |
| Sse9I | AATT | 1 cut(s) | 42 |
| SseBI | AGGCCT | 1 cut(s) | 170 |
| StuI | AGGCCT | 1 cut(s) | 170 |
| TasI | AATT | 1 cut(s) | 42 |
| Tru1I | TTAA | 1 cut(s) | 120 |
| Tru9I | TTAA | 1 cut(s) | 120 |
| TscAI | CASTG | 1 cut(s) | 86 |
| TseI | GCWGC | 2 cut(s) | 113, 188 |
| TspRI | CASTG | 1 cut(s) | 86 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 34 |
| XceI | RCATGY | 1 cut(s) | 179 |