Rmu_sc0002565.1_g000003

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002565.1
OG0017115
Physical Location & Seq
Reverse (-)
3862 .. 5433
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1572 bp
atgtgtagccaaaccgagactcaaagctcaattctggaagagatggaattcttagtactattgctttctttgttcctgagtttagtttcatgtgcaagttctgatccaaccactgaaactctcaggctgtgcatgacaactcaactcggtgactacaccgaaacgtctcaaatcatccacacctcaaactcatcttcgtattcatctatcttgaaatcctcacaacaaaatcctagatggctaaactccacatcaaagcctctcctcattctcacacctttcaacgagtccgaaatccgagcagctgtgctttgcagcagaaaacgtggcctacaaattagggtcagaagtggaggccatgactatgaagggctctcatatctctgcaagacgccattcatcatcattgacatgatcaacttcaagtccattgatgtcaaccttgctgatgaaacagcttgggttcagtcaggagctacgctaggtgagctttactatagcattgggaagaaaagtgacgtccatggattcccagctgggatttgtccaactgttggaattggtggtcattttagtggtggtggatttgggaccttgattaggaaacatggcctagcagctgaccatgtcatagatgctattttaattgatgctaatgggaaaatcatgaacagaaaaacaatgggggaggatctgttttgggccattagaggaggtggaggagcaagttttgggatcatagtttcatggaagatcaaattggttcaggtcccaaaagttgtgactggttttacagttaacaagttaatatcacaaggtggaagtagccttgtgaataggtggcagtacattgcagacaagttccatgaggatcttttcatgagagtcatccttcagaatgttgggagtggaaatcagaaacaagtccaagccgctttcaattctttgtttctaggagggattgagacactaatgccattgatgaaacagagctttccagagttgggtttggcggcaaaagactgcattgaaatgagttgggtccaatctgctcagtactttgctgggtacaaaaaagagcagcctttggaggtcttgttgtctagggaggcactctataagagcaatttcaaggccaaatccgactttgtaataaaccctattccagaaatcgggctacgagggatttgggaaaggttcatgctagaagaactagctttcatgatactggatccctatggtggaaagatggctgagatttcggaattcgaaattccatttcctcataggaaggggaatctgtacaacatacagtacattgtgaagtgggatgtgaatgaggtcagtgaagccaacaagcatatgaattggatgaatatgctttatgggtttatgaagccctatgtttcaaattctccaaggggtgcctacatcaattacaaggatcttcagttggggaccaacaacaaaggcactaacacgagctacatagaagcgagtagttgggggagaaagtacttcaaaaacttgatcttcgcgagccaaccaactcatccacactcgcgtccttga

Protein Analysis

523

Amino Acids

58.46

Weight (kDa)

8.47

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 87 - 223 6.4e-23 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20860
fragaria_vesca FvH4_3g10830
malus_domestica MD10G1247300.v1.1
prunus_persica Prupe.4G096000_v2.0.a1 Prupe.4G096100_v2.0.a1
pyrus_communis pycom05g25170 pycom10g20520
rosa_chinensis RchiOBHm_Chr5g0017541
rosa_laevigata RLG00000032297
rosa_multiflora Rmu_sc0002565.1_g000003
rosa_roxburghii Rroxscaffold_1G00059900
rosa_rugosa Rorug05G0036800
rosa_wichuraiana Rw5G011300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 522
AccB1I GGYRCC 1 cut(s) 1424
AccB7I CCANNNNNTGG 1 cut(s) 554
AccII CGCG 2 cut(s) 1538, 1564
AciI CCGC 2 cut(s) 933, 1013
AclWI GGATC 7 cut(s) 98, 699, 743, 879, 1226, 1239, 1452
AcsI RAATTY 4 cut(s) 47, 1265, 1272, 1411
AcuI CTGAAG 2 cut(s) 878, 1433
AcyI GRCGYC 2 cut(s) 392, 519
AdeI CACNNNGTG 1 cut(s) 818
AfaI GTAC 7 cut(s) 57, 848, 1058, 1070, 1304, 1316, 1517
AfiI CCNNNNNNNGG 5 cut(s) 554, 600, 1004, 1172, 1241
AgsI TTSAA 8 cut(s) 214, 283, 424, 940, 1031, 1132, 1410, 1522
AjuI GAANNNNNNNTTGG 2 cut(s) 743, 775
Alw26I GTCTC 3 cut(s) 11, 171, 959
AlwI GGATC 7 cut(s) 98, 699, 743, 879, 1226, 1239, 1452
AoxI GGCC 5 cut(s) 328, 355, 610, 702, 1134
ApeKI GCWGC 4 cut(s) 302, 315, 617, 1081
ApoI RAATTY 4 cut(s) 47, 1265, 1272, 1411
AspS9I GGNCC 5 cut(s) 591, 702, 769, 1042, 1458
AsuHPI GGTGA 2 cut(s) 161, 497
AsuII TTCGAA 1 cut(s) 1269
AvaII GGWCC 4 cut(s) 591, 769, 1042, 1458
BamHI GGATCC 1 cut(s) 1231
BanI GGYRCC 1 cut(s) 1424
BanII GRGCYC 1 cut(s) 375
BauI CACGAG 1 cut(s) 1480
BbvI GCAGC 4 cut(s) 314, 327, 629, 1093
BccI CCATC 3 cut(s) 37, 231, 1243
BclI TGATCA 1 cut(s) 414
BcoDI GTCTC 3 cut(s) 11, 171, 959
BfaI CTAG 7 cut(s) 234, 482, 614, 953, 1104, 1205, 1214
BfmI CTRYAG 1 cut(s) 496
BisI GCNGC 6 cut(s) 303, 316, 618, 933, 1014, 1082
BlsI GCNGC 6 cut(s) 304, 317, 619, 934, 1015, 1083
BmcAI AGTACT 3 cut(s) 57, 1058, 1517
Bme18I GGWCC 4 cut(s) 591, 769, 1042, 1458
BmgT120I GGNCC 5 cut(s) 591, 702, 769, 1042, 1458
BmiI GGNNCC 6 cut(s) 592, 771, 1043, 1233, 1426, 1459
BmsI GCATC 2 cut(s) 625, 640
Bpu14I TTCGAA 1 cut(s) 1269
BsaHI GRCGYC 2 cut(s) 392, 519
BsaJI CCNNGG 2 cut(s) 523, 1418
BsaXI ACNNNNNCTCC 4 cut(s) 680, 710, 1082, 1112
Bsc4I CCNNNNNNNGG 5 cut(s) 554, 600, 1004, 1172, 1241
Bse1I ACTGG 2 cut(s) 790, 1233
Bse3DI GCAATG 1 cut(s) 849
BseDI CCNNGG 2 cut(s) 523, 1418
BseGI GGATG 5 cut(s) 174, 888, 1336, 1377, 1552
BseLI CCNNNNNNNGG 5 cut(s) 554, 600, 1004, 1172, 1241
BseMI GCAATG 1 cut(s) 849
BseMII CTCAG 4 cut(s) 68, 136, 1067, 1245
BseNI ACTGG 2 cut(s) 790, 1233
BseRI GAGGAG 3 cut(s) 254, 726, 735
BseXI GCAGC 4 cut(s) 314, 327, 629, 1093
BseYI CCCAGC 3 cut(s) 532, 536, 1064
Bsh1236I CGCG 2 cut(s) 1538, 1564
BshFI GGCC 5 cut(s) 330, 357, 612, 704, 1136
BshNI GGYRCC 1 cut(s) 1424
BslFI GGGAC 3 cut(s) 604, 755, 1471
BslI CCNNNNNNNGG 5 cut(s) 554, 600, 1004, 1172, 1241
BsmAI GTCTC 3 cut(s) 11, 171, 959
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 3 cut(s) 604, 755, 1471
BsnI GGCC 5 cut(s) 330, 357, 612, 704, 1136
Bsp119I TTCGAA 1 cut(s) 1269
Bsp1286I GDGCHC 1 cut(s) 375
Bsp1407I TGTACA 1 cut(s) 1302
Bsp143I GATC 9 cut(s) 103, 414, 691, 735, 753, 871, 1231, 1444, 1530
Bsp19I CCATGG 1 cut(s) 523
Bsp68I TCGCGA 1 cut(s) 1538
BspACI CCGC 2 cut(s) 933, 1013
BspANI GGCC 5 cut(s) 330, 357, 612, 704, 1136
BspCNI CTCAG 4 cut(s) 69, 135, 1066, 1246
BspFNI CGCG 2 cut(s) 1538, 1564
BspHI TCATGA 3 cut(s) 666, 879, 1221
BspLI GGNNCC 6 cut(s) 592, 771, 1043, 1233, 1426, 1459
BspPI GGATC 7 cut(s) 98, 699, 743, 879, 1226, 1239, 1452
BspT104I TTCGAA 1 cut(s) 1269
BspT107I GGYRCC 1 cut(s) 1424
BsrDI GCAATG 1 cut(s) 849
BsrGI TGTACA 1 cut(s) 1302
BsrI ACTGG 2 cut(s) 790, 1233
BssECI CCNNGG 2 cut(s) 523, 1418
BssMI GATC 9 cut(s) 103, 414, 691, 735, 753, 871, 1231, 1444, 1530
BssNI GRCGYC 2 cut(s) 392, 519
BssSI CACGAG 1 cut(s) 1480
BssT1I CCWWGG 2 cut(s) 523, 1418
Bst2BI CACGAG 1 cut(s) 1480
Bst4CI ACNGT 3 cut(s) 553, 796, 1314
Bst6I CTCTTC 1 cut(s) 33
BstACI GRCGYC 2 cut(s) 392, 519
BstAUI TGTACA 1 cut(s) 1302
BstBI TTCGAA 1 cut(s) 1269
BstC8I GCNNGC 1 cut(s) 1540
BstDEI CTNAG 5 cut(s) 52, 77, 122, 1053, 1254
BstDSI CCRYGG 1 cut(s) 523
BstENI CCTNNNNNAGG 1 cut(s) 598
BstF5I GGATG 5 cut(s) 174, 888, 1336, 1377, 1552
BstFNI CGCG 2 cut(s) 1538, 1564
BstKTI GATC 9 cut(s) 106, 417, 694, 738, 756, 874, 1234, 1447, 1533
BstMAI GTCTC 3 cut(s) 11, 171, 959
BstMBI GATC 9 cut(s) 103, 414, 691, 735, 753, 871, 1231, 1444, 1530
BstMWI GCNNNNNNNGC 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 496
BstUI CGCG 2 cut(s) 1538, 1564
BstV1I GCAGC 4 cut(s) 314, 327, 629, 1093
BstX2I RGATCY 4 cut(s) 691, 871, 1231, 1444
BstYI RGATCY 4 cut(s) 691, 871, 1231, 1444
BsuRI GGCC 5 cut(s) 330, 357, 612, 704, 1136
BtgI CCRYGG 1 cut(s) 523
BtsCI GGATG 5 cut(s) 174, 888, 1336, 1377, 1552
BtsIMutI CAGTG 2 cut(s) 111, 1351
BtuMI TCGCGA 1 cut(s) 1538
Cac8I GCNNGC 1 cut(s) 1540
CciI TCATGA 3 cut(s) 666, 879, 1221
Cfr13I GGNCC 5 cut(s) 591, 702, 769, 1042, 1458
CseI GACGC 2 cut(s) 400, 1553
Csp6I GTAC 7 cut(s) 56, 847, 1057, 1069, 1303, 1315, 1516
CviQI GTAC 7 cut(s) 56, 847, 1057, 1069, 1303, 1315, 1516
DdeI CTNAG 5 cut(s) 52, 77, 122, 1053, 1254
DpnI GATC 9 cut(s) 105, 416, 693, 737, 755, 873, 1233, 1446, 1532
DpnII GATC 9 cut(s) 103, 414, 691, 735, 753, 871, 1231, 1444, 1530
DraIII CACNNNGTG 1 cut(s) 818
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Eco130I CCWWGG 2 cut(s) 523, 1418
Eco24I GRGCYC 1 cut(s) 375
Eco47I GGWCC 4 cut(s) 591, 769, 1042, 1458
Eco57I CTGAAG 2 cut(s) 878, 1433
EcoNI CCTNNNNNAGG 1 cut(s) 598
EcoO109I RGGNCCY 2 cut(s) 591, 769
EcoRI GAATTC 2 cut(s) 47, 1265
EcoT14I CCWWGG 2 cut(s) 523, 1418
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 2 cut(s) 523, 1418
Esp3I CGTCTC 1 cut(s) 171
FaqI GGGAC 3 cut(s) 604, 755, 1471
FauNDI CATATG 1 cut(s) 1362
FbaI TGATCA 1 cut(s) 414
Fnu4HI GCNGC 6 cut(s) 303, 316, 618, 933, 1014, 1082
FokI GGATG 5 cut(s) 161, 875, 1343, 1384, 1539
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 6 cut(s) 303, 316, 618, 933, 1014, 1082
FspBI CTAG 7 cut(s) 234, 482, 614, 953, 1104, 1205, 1214
GluI GCNGC 6 cut(s) 303, 316, 618, 933, 1014, 1082
GsaI CCCAGC 3 cut(s) 536, 540, 1068
HaeIII GGCC 5 cut(s) 330, 357, 612, 704, 1136
HgaI GACGC 2 cut(s) 400, 1553
Hin1I GRCGYC 2 cut(s) 392, 519
HincII GTYRAC 2 cut(s) 439, 799
HindII GTYRAC 2 cut(s) 439, 799
HinfI GANTC 5 cut(s) 19, 287, 528, 885, 1297
HpaI GTTAAC 1 cut(s) 799
HphI GGTGA 2 cut(s) 161, 497
Hpy166II GTNNAC 2 cut(s) 439, 799
Hpy188I TCNGA 8 cut(s) 103, 292, 299, 347, 897, 918, 1144, 1264
Hpy8I GTNNAC 2 cut(s) 439, 799
HpyAV CCTTC 3 cut(s) 362, 902, 1285
HpyCH4III ACNGT 3 cut(s) 553, 796, 1314
HpyCH4IV ACGT 3 cut(s) 164, 325, 519
HpyCH4V TGCA 6 cut(s) 95, 132, 315, 387, 854, 1026
HpyF10VI GCNNNNNNNGC 1 cut(s) 487
HpyF3I CTNAG 5 cut(s) 52, 77, 122, 1053, 1254
HpySE526I ACGT 3 cut(s) 164, 325, 519
Hsp92I GRCGYC 2 cut(s) 392, 519
Ksp22I TGATCA 1 cut(s) 414
KspAI GTTAAC 1 cut(s) 799
Kzo9I GATC 9 cut(s) 103, 414, 691, 735, 753, 871, 1231, 1444, 1530
LmnI GCTCC 2 cut(s) 473, 722
Lsp1109I GCAGC 4 cut(s) 314, 327, 629, 1093
LweI GCATC 2 cut(s) 625, 640
MaeI CTAG 7 cut(s) 234, 482, 614, 953, 1104, 1205, 1214
MaeII ACGT 3 cut(s) 164, 325, 519
MaeIII GTNAC 3 cut(s) 149, 515, 781
MalI GATC 9 cut(s) 105, 416, 693, 737, 755, 873, 1233, 1446, 1532
MboI GATC 9 cut(s) 103, 414, 691, 735, 753, 871, 1231, 1444, 1530
MboII GAAGA 7 cut(s) 50, 186, 520, 763, 1220, 1439, 1525
MflI RGATCY 4 cut(s) 691, 871, 1231, 1444
MhlI GDGCHC 1 cut(s) 375
MlyI GAGTC 3 cut(s) 13, 296, 894
MmeI TCCRAC 4 cut(s) 131, 535, 572, 1167
MseI TTAA 3 cut(s) 644, 798, 806
MslI CAYNNNNRTG 4 cut(s) 363, 410, 573, 1031
MspA1I CMGCKG 3 cut(s) 305, 536, 620
MvnI CGCG 2 cut(s) 1538, 1564
MwoI GCNNNNNNNGC 1 cut(s) 487
NcoI CCATGG 1 cut(s) 523
NdeI CATATG 1 cut(s) 1362
NdeII GATC 9 cut(s) 103, 414, 691, 735, 753, 871, 1231, 1444, 1530
NlaIV GGNNCC 6 cut(s) 592, 771, 1043, 1233, 1426, 1459
NmuCI GTSAC 3 cut(s) 149, 515, 781
NruI TCGCGA 1 cut(s) 1538
NspV TTCGAA 1 cut(s) 1269
PagI TCATGA 3 cut(s) 666, 879, 1221
PfeI GAWTC 2 cut(s) 528, 1297
PflFI GACNNNGTC 1 cut(s) 626
PflMI CCANNNNNTGG 1 cut(s) 554
PkrI GCNGC 6 cut(s) 304, 317, 619, 934, 1015, 1083
PleI GAGTC 3 cut(s) 13, 295, 893
PpsI GAGTC 3 cut(s) 13, 295, 893
PpuMI RGGWCCY 2 cut(s) 591, 769
Psp5II RGGWCCY 2 cut(s) 591, 769
PspFI CCCAGC 3 cut(s) 532, 536, 1064
PspN4I GGNNCC 6 cut(s) 592, 771, 1043, 1233, 1426, 1459
PspPI GGNCC 5 cut(s) 591, 702, 769, 1042, 1458
PspPPI RGGWCCY 2 cut(s) 591, 769
PsuI RGATCY 4 cut(s) 691, 871, 1231, 1444
PsyI GACNNNGTC 1 cut(s) 626
PvuII CAGCTG 3 cut(s) 305, 536, 620
RruI TCGCGA 1 cut(s) 1538
RsaI GTAC 7 cut(s) 57, 848, 1058, 1070, 1304, 1316, 1517
RsaNI GTAC 7 cut(s) 56, 847, 1057, 1069, 1303, 1315, 1516
RseI CAYNNNNRTG 4 cut(s) 363, 410, 573, 1031
SaqAI TTAA 3 cut(s) 644, 798, 806
SatI GCNGC 6 cut(s) 303, 316, 618, 933, 1014, 1082
Sau3AI GATC 9 cut(s) 103, 414, 691, 735, 753, 871, 1231, 1444, 1530
Sau96I GGNCC 5 cut(s) 591, 702, 769, 1042, 1458
ScaI AGTACT 3 cut(s) 57, 1058, 1517
SchI GAGTC 3 cut(s) 13, 296, 894
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 2 cut(s) 625, 640
SfcI CTRYAG 1 cut(s) 496
SfuI TTCGAA 1 cut(s) 1269
SinI GGWCC 4 cut(s) 591, 769, 1042, 1458
SmiMI CAYNNNNRTG 4 cut(s) 363, 410, 573, 1031
SsiI CCGC 2 cut(s) 933, 1013
SspMI CTAG 7 cut(s) 234, 482, 614, 953, 1104, 1205, 1214
StyI CCWWGG 2 cut(s) 523, 1418
TaaI ACNGT 3 cut(s) 553, 796, 1314
TaiI ACGT 3 cut(s) 167, 328, 522
TaqI TCGA 1 cut(s) 1269
TatI WGTACW 6 cut(s) 55, 846, 1056, 1302, 1314, 1515
TauI GCSGC 2 cut(s) 935, 1016
TfiI GAWTC 2 cut(s) 528, 1297
Tru1I TTAA 3 cut(s) 644, 798, 806
Tru9I TTAA 3 cut(s) 644, 798, 806
TscAI CASTG 2 cut(s) 118, 1351
TseFI GTSAC 3 cut(s) 149, 515, 781
TseI GCWGC 4 cut(s) 302, 315, 617, 1081
Tsp45I GTSAC 3 cut(s) 149, 515, 781
TspRI CASTG 2 cut(s) 118, 1351
Tth111I GACNNNGTC 1 cut(s) 626
Van91I CCANNNNNTGG 1 cut(s) 554
VpaK11BI GGWCC 4 cut(s) 591, 769, 1042, 1458
XagI CCTNNNNNAGG 1 cut(s) 598
XapI RAATTY 4 cut(s) 47, 1265, 1272, 1411
XspI CTAG 7 cut(s) 234, 482, 614, 953, 1104, 1205, 1214
ZraI GACGTC 1 cut(s) 520
ZrmI AGTACT 3 cut(s) 57, 1058, 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.