Rmu_sc0003058.1_g000002

Kynurenine--oxoglutarate transaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003058.1
OG0012279
Physical Location & Seq
Reverse (-)
2881 .. 5529
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1335 bp
atgcgcttgtgttcttcaaccctcttcaccttttccaaaaattcgcacaaagacttgagctttattaggacgaggacaaccacggtctgccctccttgcccttcggtctcagccacaatgtccaccgtttcgacagaaaaggatgccctttcgggtcaaacagaatccacccagaagccattacaggtggcaaagcggttggaaaaattcaagacgacaatcttcacgcaaatgagtaatcttgctgttaaacatggagcaataaaccttgggcaagggtttcccaactttgatggtccggagtttgtaaaggaagctgcaattcaggccgtgaaagatgggaaaaatcaatatgctcgtggatatggggttccagacctaaacaatgccattgctgagagattcaagaaagacactggactggttgtggaccctgagaaggaaataactgttacctctgggtgcacagaggcaattgcagcaaccatgttgggcttgataaatcctggtgatgaggttatcctctttgctcctttttatgattcttacgaagcgactctttccatggctggtgctaaagtaaaaggtatcacactacgccctccagattttgctgtccccattgatgaactgaagtctgcaatctccaagaatactcgtgcaattcttataaatactccgcataatcctactggaaagatgttcactcgagaggaacttgacgctattgcatctctctgcattgaaaatgatgtactggtgttcactgatgaagtttatgataagttggcttttgaaatggatcacatttctatggcttctcttcctggaatgtacgagcgtacagtaaccctgaattccttggggaaaacattttcgttaactgggtggaagatcggttgggctatagctcccccccacctgtcatggggagtgcggcaggcacactcttatctcacttttgctacctccactcctatgcaatgggctgctgcaacagctctcagagccccagactcctactttctggagctgaaaagagattacttggcaaagaaggaaattttggtagagggattgaaggctgttggttttaaagtgtatccatccagcgggacctactttgtggttgtagatcacacttcttttgggttgccaaatgatgttgcattttgtgagtatctgatcaaggaagtcggagtggtggcaatccctaccagtgtgttttatttaaacccagaagatggaaagaatctagtcagatttactttctgcaaggatgagggaactcttagggctgcagttgagaggatgaaggagaagttgaaaaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0008144 GO:0008483 GO:0010326 GO:0016740 GO:0016769 GO:0019842 GO:0030170 GO:0036094 GO:0043167 GO:0043168 GO:0048037 GO:0050662 GO:0070279 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

444

Amino Acids

48.87

Weight (kDa)

7.04

Isoelectric Point (pI)

28.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 88 - 437 2.3e-68 Aminotransferase class I and II
Cys_Met_Meta_PP PF01053 128 - 259 1.8e-08 Cys/Met metabolism PLP-dependent enzyme
Aminotran_5 PF00266 143 - 259 1.3e-07 Aminotransferase class-V
DegT_DnrJ_EryC1 PF01041 143 - 257 8e-07 DegT/DnrJ/EryC1/StrS aminotransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77670
fragaria_vesca FvH4_2g26550 FvH4_2g26550 FvH4_2g26550
malus_domestica MD08G1166900.v1.1 MD15G1353000.v1.1
prunus_persica Prupe.1G501400_v2.0.a1
pyrus_communis pycom08g14300 pycom15g31620
rosa_chinensis RchiOBHm_Chr6g0295421
rosa_laevigata RLG00000011805
rosa_multiflora Rmu_sc0003058.1_g000002
rosa_roxburghii Rroxscaffold_7G00172510
rosa_rugosa Rorug06G0250500
rosa_samantha Rh6AG363900 Rh6BG372100 Rh6CG378500 Rh6DG364900
rosa_wichuraiana Rw6G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 671
AccB7I CCANNNNNTGG 1 cut(s) 1244
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 4 cut(s) 196, 680, 937, 1113
AclWI GGATC 1 cut(s) 810
AcsI RAATTY 4 cut(s) 40, 206, 856, 1062
AcuI CTGAAG 1 cut(s) 653
AfaI GTAC 3 cut(s) 756, 836, 844
AfiI CCNNNNNNNGG 4 cut(s) 439, 928, 1112, 1244
AgsI TTSAA 7 cut(s) 18, 211, 406, 746, 797, 1081, 1327
AjnI CCWGG 2 cut(s) 505, 826
AjuI GAANNNNNNNTTGG 2 cut(s) 1049, 1081
AloI GAACNNNNNNTCC 2 cut(s) 354, 386
AluBI AGCT 5 cut(s) 60, 317, 911, 1001, 1033
AluI AGCT 5 cut(s) 60, 317, 911, 1001, 1033
Alw21I GWGCWC 1 cut(s) 467
Alw26I GTCTC 1 cut(s) 112
Alw44I GTGCAC 1 cut(s) 463
AlwI GGATC 1 cut(s) 810
Ama87I CYCGRG 1 cut(s) 708
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 1 cut(s) 327
ApaLI GTGCAC 1 cut(s) 463
ApeKI GCWGC 5 cut(s) 317, 479, 989, 992, 1298
ApoI RAATTY 4 cut(s) 40, 206, 856, 1062
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 3 cut(s) 296, 430, 1116
AsuHPI GGTGA 2 cut(s) 19, 521
AvaI CYCGRG 1 cut(s) 708
AvaII GGWCC 3 cut(s) 296, 430, 1116
BaeGI GKGCMC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 1012
BauI CACGAG 2 cut(s) 357, 657
Bbv12I GWGCWC 1 cut(s) 467
BbvI GCAGC 5 cut(s) 304, 491, 976, 979, 1285
BccI CCATC 4 cut(s) 287, 332, 1114, 1238
BceAI ACGGC 1 cut(s) 314
BciT130I CCWGG 2 cut(s) 507, 828
BciVI GTATCC 1 cut(s) 1113
BclI TGATCA 1 cut(s) 1185
BcoDI GTCTC 1 cut(s) 112
BfaI CTAG 1 cut(s) 1256
BfmI CTRYAG 2 cut(s) 906, 1299
BfuI GTATCC 1 cut(s) 1113
BisI GCNGC 6 cut(s) 318, 480, 938, 990, 993, 1299
BlsI GCNGC 6 cut(s) 319, 481, 939, 991, 994, 1300
Bme1390I CCNGG 2 cut(s) 507, 828
Bme18I GGWCC 3 cut(s) 296, 430, 1116
BmeT110I CYCGRG 1 cut(s) 708
BmgT120I GGNCC 3 cut(s) 296, 430, 1116
BmiI GGNNCC 3 cut(s) 372, 432, 1117
BmrFI CCNGG 2 cut(s) 507, 828
BmrI ACTGGG 1 cut(s) 894
BmsI GCATC 2 cut(s) 133, 740
BmuI ACTGGG 1 cut(s) 894
BpmI CTGGAG 2 cut(s) 588, 1049
BpuEI CTTGAG 1 cut(s) 76
BsaI GGTCTC 1 cut(s) 112
BsaJI CCNNGG 4 cut(s) 81, 268, 564, 861
BsaWI WCCGGW 1 cut(s) 298
BsaXI ACNNNNNCTCC 2 cut(s) 958, 988
Bsc4I CCNNNNNNNGG 4 cut(s) 439, 928, 1112, 1244
Bse1I ACTGG 6 cut(s) 421, 426, 697, 762, 889, 1218
Bse3DI GCAATG 2 cut(s) 390, 989
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 2 cut(s) 507, 828
BseDI CCNNGG 4 cut(s) 81, 268, 564, 861
BseGI GGATG 4 cut(s) 148, 1106, 1285, 1317
BseLI CCNNNNNNNGG 4 cut(s) 439, 928, 1112, 1244
BseMI GCAATG 2 cut(s) 390, 989
BseMII CTCAG 4 cut(s) 123, 387, 426, 1018
BseNI ACTGG 6 cut(s) 421, 426, 697, 762, 889, 1218
BseSI GKGCMC 1 cut(s) 467
BseXI GCAGC 5 cut(s) 304, 491, 976, 979, 1285
BshFI GGCC 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 467
BsiHKCI CYCGRG 1 cut(s) 708
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 2 cut(s) 602, 1129
BslI CCNNNNNNNGG 4 cut(s) 439, 928, 1112, 1244
BsmAI GTCTC 1 cut(s) 112
BsmFI GGGAC 2 cut(s) 602, 1129
BsnI GGCC 1 cut(s) 329
Bso31I GGTCTC 1 cut(s) 112
BsoBI CYCGRG 1 cut(s) 708
Bsp1286I GDGCHC 2 cut(s) 467, 1012
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 4 cut(s) 802, 894, 1135, 1185
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 4 cut(s) 196, 680, 937, 1113
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 4 cut(s) 122, 388, 427, 1017
BspEI TCCGGA 1 cut(s) 298
BspLI GGNNCC 3 cut(s) 372, 432, 1117
BspMAI CTGCAG 1 cut(s) 1303
BspPI GGATC 1 cut(s) 810
BspTNI GGTCTC 1 cut(s) 112
BsrDI GCAATG 2 cut(s) 390, 989
BsrI ACTGG 6 cut(s) 421, 426, 697, 762, 889, 1218
BssECI CCNNGG 4 cut(s) 81, 268, 564, 861
BssMI GATC 4 cut(s) 802, 894, 1135, 1185
BssSI CACGAG 2 cut(s) 357, 657
BssT1I CCWWGG 3 cut(s) 268, 564, 861
Bst2BI CACGAG 2 cut(s) 357, 657
Bst2UI CCWGG 2 cut(s) 507, 828
Bst4CI ACNGT 4 cut(s) 85, 127, 451, 847
Bst6I CTCTTC 2 cut(s) 29, 828
BstC8I GCNNGC 1 cut(s) 942
BstDEI CTNAG 5 cut(s) 109, 396, 435, 1004, 1292
BstDSI CCRYGG 2 cut(s) 81, 564
BstF5I GGATG 4 cut(s) 148, 1106, 1285, 1317
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 4 cut(s) 805, 897, 1138, 1188
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 4 cut(s) 802, 894, 1135, 1185
BstMWI GCNNNNNNNGC 5 cut(s) 96, 326, 479, 998, 1007
BstNI CCWGG 2 cut(s) 507, 828
BstSCI CCNGG 2 cut(s) 505, 826
BstSFI CTRYAG 2 cut(s) 906, 1299
BstSLI GKGCMC 1 cut(s) 467
BstV1I GCAGC 5 cut(s) 304, 491, 976, 979, 1285
BsuI GTATCC 1 cut(s) 1113
BsuRI GGCC 1 cut(s) 329
BtgI CCRYGG 2 cut(s) 81, 564
BtsCI GGATG 4 cut(s) 148, 1106, 1285, 1317
BtsIMutI CAGTG 3 cut(s) 414, 765, 1225
Cac8I GCNNGC 1 cut(s) 942
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 3 cut(s) 296, 430, 1116
CseI GACGC 1 cut(s) 731
Csp6I GTAC 3 cut(s) 755, 835, 843
CviAII CATG 4 cut(s) 254, 487, 565, 927
CviQI GTAC 3 cut(s) 755, 835, 843
DdeI CTNAG 5 cut(s) 109, 396, 435, 1004, 1292
DpnI GATC 4 cut(s) 804, 896, 1137, 1187
DpnII GATC 4 cut(s) 802, 894, 1135, 1185
DraI TTTAAA 2 cut(s) 1096, 1233
Eam1104I CTCTTC 2 cut(s) 29, 828
EarI CTCTTC 2 cut(s) 29, 828
Eco130I CCWWGG 3 cut(s) 268, 564, 861
Eco24I GRGCYC 1 cut(s) 1012
Eco31I GGTCTC 1 cut(s) 112
Eco47I GGWCC 3 cut(s) 296, 430, 1116
Eco57I CTGAAG 1 cut(s) 653
Eco88I CYCGRG 1 cut(s) 708
EcoO109I RGGNCCY 1 cut(s) 1116
EcoRI GAATTC 1 cut(s) 856
EcoRII CCWGG 2 cut(s) 505, 826
EcoT14I CCWWGG 3 cut(s) 268, 564, 861
EcoT38I GRGCYC 1 cut(s) 1012
ErhI CCWWGG 3 cut(s) 268, 564, 861
FaeI CATG 4 cut(s) 257, 490, 568, 930
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FaqI GGGAC 2 cut(s) 602, 1129
FatI CATG 4 cut(s) 253, 486, 564, 926
FauI CCCGC 1 cut(s) 1106
FbaI TGATCA 1 cut(s) 1185
Fnu4HI GCNGC 6 cut(s) 318, 480, 938, 990, 993, 1299
FokI GGATG 4 cut(s) 155, 1093, 1292, 1324
FriOI GRGCYC 1 cut(s) 1012
Fsp4HI GCNGC 6 cut(s) 318, 480, 938, 990, 993, 1299
FspBI CTAG 1 cut(s) 1256
GlaI GCGC 1 cut(s) 5
GluI GCNGC 6 cut(s) 318, 480, 938, 990, 993, 1299
GsuI CTGGAG 2 cut(s) 588, 1049
HaeIII GGCC 1 cut(s) 329
HapII CCGG 1 cut(s) 299
HgaI GACGC 1 cut(s) 731
HhaI GCGC 1 cut(s) 6
Hin1II CATG 4 cut(s) 257, 490, 568, 930
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HinfI GANTC 6 cut(s) 164, 402, 542, 556, 1016, 1252
HpaI GTTAAC 1 cut(s) 882
HpaII CCGG 1 cut(s) 299
HphI GGTGA 2 cut(s) 19, 521
Hpy166II GTNNAC 6 cut(s) 123, 430, 465, 705, 765, 882
Hpy188I TCNGA 4 cut(s) 1007, 1185, 1199, 1262
Hpy188III TCNNGA 7 cut(s) 211, 299, 374, 406, 605, 710, 1028
Hpy8I GTNNAC 6 cut(s) 123, 430, 465, 705, 765, 882
HpyAV CCTTC 5 cut(s) 111, 433, 1051, 1075, 1309
HpyCH4III ACNGT 4 cut(s) 85, 127, 451, 847
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 326, 479, 998, 1007
HpyF3I CTNAG 5 cut(s) 109, 396, 435, 1004, 1292
Hsp92II CATG 4 cut(s) 257, 490, 568, 930
HspAI GCGC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 1185
KspAI GTTAAC 1 cut(s) 882
Kzo9I GATC 4 cut(s) 802, 894, 1135, 1185
LmnI GCTCC 4 cut(s) 257, 535, 916, 1030
Lsp1109I GCAGC 5 cut(s) 304, 491, 976, 979, 1285
LweI GCATC 2 cut(s) 133, 740
MaeI CTAG 1 cut(s) 1256
MaeIII GTNAC 2 cut(s) 451, 847
MalI GATC 4 cut(s) 804, 896, 1137, 1187
MboI GATC 4 cut(s) 802, 894, 1135, 1185
MboII GAAGA 6 cut(s) 6, 16, 214, 815, 904, 1253
MfeI CAATTG 1 cut(s) 474
MhlI GDGCHC 2 cut(s) 467, 1012
MluCI AATT 7 cut(s) 40, 206, 321, 474, 663, 856, 1062
MlyI GAGTC 2 cut(s) 550, 1010
MmeI TCCRAC 2 cut(s) 180, 1177
MroI TCCGGA 1 cut(s) 298
MseI TTAA 4 cut(s) 249, 881, 1095, 1232
MslI CAYNNNNRTG 3 cut(s) 230, 812, 977
MspA1I CMGCKG 1 cut(s) 1113
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 2 cut(s) 507, 828
MunI CAATTG 1 cut(s) 474
MvaI CCWGG 2 cut(s) 507, 828
MwoI GCNNNNNNNGC 5 cut(s) 96, 326, 479, 998, 1007
NcoI CCATGG 1 cut(s) 564
NdeII GATC 4 cut(s) 802, 894, 1135, 1185
NlaIII CATG 4 cut(s) 257, 490, 568, 930
NlaIV GGNNCC 3 cut(s) 372, 432, 1117
PaeR7I CTCGAG 1 cut(s) 708
PfeI GAWTC 4 cut(s) 164, 402, 542, 1252
PflMI CCANNNNNTGG 1 cut(s) 1244
PfoI TCCNGGA 1 cut(s) 826
PkrI GCNGC 6 cut(s) 319, 481, 939, 991, 994, 1300
PleI GAGTC 2 cut(s) 550, 1010
PpsI GAGTC 2 cut(s) 550, 1010
PpuMI RGGWCCY 1 cut(s) 1116
PsiI TTATAA 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 1116
Psp6I CCWGG 2 cut(s) 505, 826
PspGI CCWGG 2 cut(s) 505, 826
PspN4I GGNNCC 3 cut(s) 372, 432, 1117
PspPI GGNCC 3 cut(s) 296, 430, 1116
PspPPI RGGWCCY 1 cut(s) 1116
PstI CTGCAG 1 cut(s) 1303
RsaI GTAC 3 cut(s) 756, 836, 844
RsaNI GTAC 3 cut(s) 755, 835, 843
RseI CAYNNNNRTG 3 cut(s) 230, 812, 977
SaqAI TTAA 4 cut(s) 249, 881, 1095, 1232
SatI GCNGC 6 cut(s) 318, 480, 938, 990, 993, 1299
Sau3AI GATC 4 cut(s) 802, 894, 1135, 1185
Sau96I GGNCC 3 cut(s) 296, 430, 1116
SchI GAGTC 2 cut(s) 550, 1010
ScrFI CCNGG 2 cut(s) 507, 828
SduI GDGCHC 2 cut(s) 467, 1012
SfaNI GCATC 2 cut(s) 133, 740
SfcI CTRYAG 2 cut(s) 906, 1299
Sfr274I CTCGAG 1 cut(s) 708
SinI GGWCC 3 cut(s) 296, 430, 1116
SlaI CTCGAG 1 cut(s) 708
SmiMI CAYNNNNRTG 3 cut(s) 230, 812, 977
SmlI CTYRAG 2 cut(s) 55, 708
SmoI CTYRAG 2 cut(s) 55, 708
Sse9I AATT 7 cut(s) 40, 206, 321, 474, 663, 856, 1062
SsiI CCGC 4 cut(s) 196, 680, 937, 1113
SspMI CTAG 1 cut(s) 1256
StyD4I CCNGG 2 cut(s) 505, 826
StyI CCWWGG 3 cut(s) 268, 564, 861
TaaI ACNGT 4 cut(s) 85, 127, 451, 847
TaqI TCGA 2 cut(s) 131, 709
TaqII GACCGA 1 cut(s) 94
TasI AATT 7 cut(s) 40, 206, 321, 474, 663, 856, 1062
TatI WGTACW 1 cut(s) 754
TauI GCSGC 1 cut(s) 940
TfiI GAWTC 4 cut(s) 164, 402, 542, 1252
Tru1I TTAA 4 cut(s) 249, 881, 1095, 1232
Tru9I TTAA 4 cut(s) 249, 881, 1095, 1232
TscAI CASTG 3 cut(s) 421, 772, 1225
TseI GCWGC 5 cut(s) 317, 479, 989, 992, 1298
TspDTI ATGAA 3 cut(s) 642, 786, 1328
TspRI CASTG 3 cut(s) 421, 772, 1225
Van91I CCANNNNNTGG 1 cut(s) 1244
VneI GTGCAC 1 cut(s) 463
VpaK11BI GGWCC 3 cut(s) 296, 430, 1116
XapI RAATTY 4 cut(s) 40, 206, 856, 1062
XhoI CTCGAG 1 cut(s) 708
XspI CTAG 1 cut(s) 1256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.