Rmu_sc0003302.1_g000003

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003302.1
OG0012812
Physical Location & Seq
Reverse (-)
8391 .. 9470
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1080 bp
atgaacagcttggaggaagaagtcgaaaagggcttattgccattgatggagtcactagctatgaaaggagaagtagtagacttgcaggagttgttgaggagattcgcgttcaatgtgatttgcaaggtgtttttgggggttgagaggtgctctttggatccctctttgcctgttccacaactcgcaagggctttcgacacggcgtcggagatctgtgcaatgcgtggggctgcccctgtgttcataatatggaagatcaagaggtggcttgggattggttcggagcagaaacttaaagctgcggtggaagaagtacatgcctatgtcatgaacataatagagacgaggaggcagaaacttgatcatgatcaaagttccgggactagtatagatcaagatcaagatcaagatctgctaactcggttaataatggcgggacatgaagacgaggtgacaagggacatggtaataagctttataatggcggggagggacactacttcagcagccatgacgtggctcttctggttgatttcccgccatcaaactgtagaggaagatttggtgaaagagattgaagacaatcaaagaacgatggatttcgagtcattggatttcagattactaaaggcatgcctttttgaatccatgaggctctttccacctgtggcttgggactcaaaacatgccaaagttcatgacttattgccggatggcactcatgtccgggccggagatagagtgacctattttccctacggaatgggaagaatggagtcgctgtgggggaaggaccggctggaattcaagccagaccggtggtttctcgaaccggacaaggagacgagatcagagctgaaaaaggtatgcccatacaagttgccgacttttcaagcaggtccaagggtttgtcttgggaaggaaatggcttttactcagatgaaatatgtgatggcttccattctcagacggttcgaaatcagaccggtctactccgacgatccggttttcgtgccacgcctgacggcccacatggccggcgggttgaaggttctggtccgcaagagagggactaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

359

Amino Acids

41.29

Weight (kDa)

6.29

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 12 - 335 5.5e-48 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012812)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01900
fragaria_vesca FvH4_6g53330
malus_domestica MD09G1011400.v1.1 MD17G1011900.v1.1
prunus_persica Prupe.3G305600_v2.0.a1
pyrus_communis pycom111g00970 pycom17g00960
rosa_chinensis RchiOBHm_Chr2g0175491
rosa_laevigata RLG00000022332
rosa_multiflora Rmu_sc0003302.1_g000003 Rmu_sc0021955.1_g000002
rosa_roxburghii Rroxscaffold_2G00077290
rosa_rugosa Rorug02G0585100
rosa_samantha Rh2AG664700 Rh2BG675400 Rh2CG638600 Rh2DG689400
rosa_wichuraiana Rw2G054480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 479
Acc36I ACCTGC 1 cut(s) 887
AccB7I CCANNNNNTGG 1 cut(s) 516
AccI GTMKAC 2 cut(s) 78, 990
AccII CGCG 1 cut(s) 107
AciI CCGC 6 cut(s) 302, 434, 485, 538, 1041, 1060
AclWI GGATC 3 cut(s) 152, 165, 995
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 1 cut(s) 803
AcuI CTGAAG 1 cut(s) 486
AcyI GRCGYC 1 cut(s) 203
AfaI GTAC 1 cut(s) 315
AfiI CCNNNNNNNGG 1 cut(s) 516
AgeI ACCGGT 2 cut(s) 816, 985
AgsI TTSAA 6 cut(s) 112, 578, 644, 808, 893, 1048
AhdI GACNNNNNGTC 1 cut(s) 202
AhlI ACTAGT 1 cut(s) 383
AjiI CACGTC 1 cut(s) 516
AluBI AGCT 5 cut(s) 9, 59, 299, 474, 856
AluI AGCT 5 cut(s) 9, 59, 299, 474, 856
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 2 cut(s) 335, 836
AlwI GGATC 3 cut(s) 152, 165, 995
AoxI GGCC 3 cut(s) 729, 1026, 1035
ApeKI GCWGC 3 cut(s) 230, 299, 506
ApoI RAATTY 1 cut(s) 803
AsiGI ACCGGT 2 cut(s) 816, 985
AspS9I GGNCC 5 cut(s) 729, 793, 899, 1027, 1057
AsuC2I CCSGG 2 cut(s) 379, 728
AsuHPI GGTGA 2 cut(s) 463, 577
AsuII TTCGAA 1 cut(s) 975
AvaII GGWCC 3 cut(s) 793, 899, 1057
BamHI GGATCC 1 cut(s) 157
BbsI GAAGAC 2 cut(s) 450, 585
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 3 cut(s) 217, 286, 518
BccI CCATC 5 cut(s) 40, 549, 589, 707, 946
BceAI ACGGC 2 cut(s) 216, 1041
BclI TGATCA 2 cut(s) 361, 367
BcnI CCSGG 2 cut(s) 379, 728
BcoDI GTCTC 2 cut(s) 335, 836
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 2 cut(s) 56, 384
BfmI CTRYAG 1 cut(s) 549
BfuAI ACCTGC 1 cut(s) 887
BglI GCCNNNNNGGC 1 cut(s) 1034
BglII AGATCT 2 cut(s) 210, 409
BisI GCNGC 3 cut(s) 231, 300, 507
BlsI GCNGC 3 cut(s) 232, 301, 508
Bme1390I CCNGG 2 cut(s) 379, 728
Bme18I GGWCC 3 cut(s) 793, 899, 1057
BmeRI GACNNNNNGTC 1 cut(s) 202
BmgBI CACGTC 1 cut(s) 516
BmgT120I GGNCC 5 cut(s) 729, 793, 899, 1027, 1057
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 2 cut(s) 379, 728
BpiI GAAGAC 2 cut(s) 450, 585
BplI GAGNNNNNCTC 2 cut(s) 134, 166
Bpu14I TTCGAA 1 cut(s) 975
BpuMI CCSGG 2 cut(s) 379, 728
BsaBI GATNNNNATC 4 cut(s) 366, 396, 402, 408
BsaHI GRCGYC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 902
BsaWI WCCGGW 4 cut(s) 816, 832, 985, 1003
BsaXI ACNNNNNCTCC 6 cut(s) 60, 90, 726, 756, 767, 797
Bsc4I CCNNNNNNNGG 1 cut(s) 516
Bse118I RCCGGY 4 cut(s) 795, 816, 985, 1037
Bse3DI GCAATG 1 cut(s) 225
Bse8I GATNNNNATC 4 cut(s) 366, 396, 402, 408
BseDI CCNNGG 1 cut(s) 902
BseGI GGATG 1 cut(s) 718
BseJI GATNNNNATC 4 cut(s) 366, 396, 402, 408
BseLI CCNNNNNNNGG 1 cut(s) 516
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 2 cut(s) 950, 979
BseRI GAGGAG 2 cut(s) 112, 361
BseXI GCAGC 3 cut(s) 217, 286, 518
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 3 cut(s) 731, 1028, 1037
BshTI ACCGGT 2 cut(s) 816, 985
BsiHKAI GWGCWC 1 cut(s) 152
BslFI GGGAC 5 cut(s) 394, 450, 473, 506, 689
BslI CCNNNNNNNGG 1 cut(s) 516
BsmAI GTCTC 2 cut(s) 335, 836
BsmBI CGTCTC 2 cut(s) 335, 836
BsmFI GGGAC 5 cut(s) 394, 450, 473, 506, 689
BsnI GGCC 3 cut(s) 731, 1028, 1037
Bsp119I TTCGAA 1 cut(s) 975
Bsp1286I GDGCHC 1 cut(s) 152
BspACI CCGC 6 cut(s) 302, 434, 485, 538, 1041, 1060
BspANI GGCC 3 cut(s) 731, 1028, 1037
BspCNI CTCAG 2 cut(s) 949, 978
BspFNI CGCG 1 cut(s) 107
BspHI TCATGA 3 cut(s) 327, 364, 697
BspLI GGNNCC 1 cut(s) 159
BspMI ACCTGC 1 cut(s) 887
BspPI GGATC 3 cut(s) 152, 165, 995
BspQI GCTCTTC 1 cut(s) 527
BspT104I TTCGAA 1 cut(s) 975
BsrDI GCAATG 1 cut(s) 225
BsrFI RCCGGY 4 cut(s) 795, 816, 985, 1037
BssAI RCCGGY 4 cut(s) 795, 816, 985, 1037
BssECI CCNNGG 1 cut(s) 902
BssNI GRCGYC 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 902
Bst4CI ACNGT 2 cut(s) 550, 972
Bst6I CTCTTC 1 cut(s) 527
BstACI GRCGYC 1 cut(s) 203
BstBI TTCGAA 1 cut(s) 975
BstC8I GCNNGC 2 cut(s) 634, 1039
BstDEI CTNAG 2 cut(s) 936, 965
BstF5I GGATG 1 cut(s) 718
BstFNI CGCG 1 cut(s) 107
BstMAI GTCTC 2 cut(s) 335, 836
BstMWI GCNNNNNNNGC 1 cut(s) 1034
BstNSI RCATGY 3 cut(s) 320, 636, 689
BstSCI CCNGG 2 cut(s) 377, 726
BstSFI CTRYAG 1 cut(s) 549
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 217, 286, 518
BstV2I GAAGAC 2 cut(s) 450, 585
BstX2I RGATCY 3 cut(s) 157, 210, 409
BstXI CCANNNNNNTGG 1 cut(s) 819
BstYI RGATCY 3 cut(s) 157, 210, 409
BsuRI GGCC 3 cut(s) 731, 1028, 1037
BtrI CACGTC 1 cut(s) 516
BtsCI GGATG 1 cut(s) 718
BveI ACCTGC 1 cut(s) 887
Cac8I GCNNGC 2 cut(s) 634, 1039
CciI TCATGA 3 cut(s) 327, 364, 697
Cfr10I RCCGGY 4 cut(s) 795, 816, 985, 1037
Cfr13I GGNCC 5 cut(s) 729, 793, 899, 1027, 1057
CseI GACGC 1 cut(s) 192
Csp6I GTAC 1 cut(s) 314
CspAI ACCGGT 2 cut(s) 816, 985
CviQI GTAC 1 cut(s) 314
DdeI CTNAG 2 cut(s) 936, 965
DriI GACNNNNNGTC 1 cut(s) 202
EaeI YGGCCR 1 cut(s) 1035
Eam1104I CTCTTC 1 cut(s) 527
Eam1105I GACNNNNNGTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 902
Eco47I GGWCC 3 cut(s) 793, 899, 1057
Eco57I CTGAAG 1 cut(s) 486
EcoRI GAATTC 1 cut(s) 803
EcoT14I CCWWGG 1 cut(s) 902
ErhI CCWWGG 1 cut(s) 902
Esp3I CGTCTC 2 cut(s) 335, 836
FaqI GGGAC 5 cut(s) 394, 450, 473, 506, 689
FauI CCCGC 4 cut(s) 427, 478, 545, 1034
FbaI TGATCA 2 cut(s) 361, 367
FblI GTMKAC 2 cut(s) 78, 990
Fnu4HI GCNGC 3 cut(s) 231, 300, 507
FokI GGATG 1 cut(s) 725
Fsp4HI GCNGC 3 cut(s) 231, 300, 507
FspBI CTAG 2 cut(s) 56, 384
GluI GCNGC 3 cut(s) 231, 300, 507
HaeIII GGCC 3 cut(s) 731, 1028, 1037
HgaI GACGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 203
HindIII AAGCTT 1 cut(s) 472
HinfI GANTC 6 cut(s) 50, 102, 605, 644, 677, 776
HphI GGTGA 2 cut(s) 463, 577
Hpy166II GTNNAC 2 cut(s) 79, 991
Hpy188I TCNGA 8 cut(s) 208, 283, 620, 853, 939, 968, 983, 997
Hpy188III TCNNGA 8 cut(s) 259, 328, 365, 395, 401, 407, 698, 827
Hpy8I GTNNAC 2 cut(s) 79, 991
Hpy99I CGWCG 2 cut(s) 208, 1001
HpyAV CCTTC 3 cut(s) 784, 913, 1042
HpyCH4III ACNGT 2 cut(s) 550, 972
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 3 cut(s) 85, 123, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 1034
HpyF3I CTNAG 2 cut(s) 936, 965
HpySE526I ACGT 1 cut(s) 515
Hsp92I GRCGYC 1 cut(s) 203
KroI GCCGGC 1 cut(s) 1037
KroNI GCCGGC 1 cut(s) 1039
Ksp22I TGATCA 2 cut(s) 361, 367
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 3 cut(s) 217, 286, 518
MaeI CTAG 2 cut(s) 56, 384
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 3 cut(s) 51, 451, 742
MboII GAAGA 8 cut(s) 29, 265, 320, 455, 514, 569, 590, 780
MflI RGATCY 3 cut(s) 157, 210, 409
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 1 cut(s) 803
MlyI GAGTC 4 cut(s) 59, 614, 671, 785
MmeI TCCRAC 2 cut(s) 186, 1020
MroNI GCCGGC 1 cut(s) 1037
MseI TTAA 2 cut(s) 294, 425
MslI CAYNNNNRTG 1 cut(s) 321
MspR9I CCNGG 2 cut(s) 379, 728
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 1 cut(s) 1034
NaeI GCCGGC 1 cut(s) 1039
NciI CCSGG 2 cut(s) 379, 728
NgoMIV GCCGGC 1 cut(s) 1037
NlaIV GGNNCC 1 cut(s) 159
NmuCI GTSAC 3 cut(s) 51, 451, 742
NspI RCATGY 3 cut(s) 320, 636, 689
NspV TTCGAA 1 cut(s) 975
PaeI GCATGC 1 cut(s) 636
PagI TCATGA 3 cut(s) 327, 364, 697
PciSI GCTCTTC 1 cut(s) 527
PdiI GCCGGC 1 cut(s) 1039
PfeI GAWTC 2 cut(s) 102, 644
PflMI CCANNNNNTGG 1 cut(s) 516
PfoI TCCNGGA 1 cut(s) 377
PinAI ACCGGT 2 cut(s) 816, 985
PkrI GCNGC 3 cut(s) 232, 301, 508
PleI GAGTC 4 cut(s) 58, 613, 671, 784
PpsI GAGTC 4 cut(s) 58, 613, 671, 784
PsiI TTATAA 1 cut(s) 479
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 5 cut(s) 729, 793, 899, 1027, 1057
PsuI RGATCY 3 cut(s) 157, 210, 409
RsaI GTAC 1 cut(s) 315
RsaNI GTAC 1 cut(s) 314
RseI CAYNNNNRTG 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 2 cut(s) 294, 425
SatI GCNGC 3 cut(s) 231, 300, 507
Sau96I GGNCC 5 cut(s) 729, 793, 899, 1027, 1057
SchI GAGTC 4 cut(s) 59, 614, 671, 785
ScrFI CCNGG 2 cut(s) 379, 728
SduI GDGCHC 1 cut(s) 152
SfcI CTRYAG 1 cut(s) 549
SfiI GGCCNNNNNGGCC 1 cut(s) 1034
SfuI TTCGAA 1 cut(s) 975
SinI GGWCC 3 cut(s) 793, 899, 1057
SmiMI CAYNNNNRTG 1 cut(s) 321
SpeI ACTAGT 1 cut(s) 383
SphI GCATGC 1 cut(s) 636
Sse9I AATT 1 cut(s) 803
SsiI CCGC 6 cut(s) 302, 434, 485, 538, 1041, 1060
SspMI CTAG 2 cut(s) 56, 384
StyD4I CCNGG 2 cut(s) 377, 726
StyI CCWWGG 1 cut(s) 902
TaaI ACNGT 2 cut(s) 550, 972
TaiI ACGT 1 cut(s) 518
TaqI TCGA 5 cut(s) 24, 195, 603, 828, 975
TasI AATT 1 cut(s) 803
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 2 cut(s) 102, 644
Tru1I TTAA 2 cut(s) 294, 425
Tru9I TTAA 2 cut(s) 294, 425
TseFI GTSAC 3 cut(s) 51, 451, 742
TseI GCWGC 3 cut(s) 230, 299, 506
Tsp45I GTSAC 3 cut(s) 51, 451, 742
TspDTI ATGAA 7 cut(s) 17, 77, 232, 344, 456, 686, 956
TspGWI ACGGA 1 cut(s) 774
Van91I CCANNNNNTGG 1 cut(s) 516
VpaK11BI GGWCC 3 cut(s) 793, 899, 1057
XapI RAATTY 1 cut(s) 803
XceI RCATGY 3 cut(s) 320, 636, 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 669
XmiI GTMKAC 2 cut(s) 78, 990
XspI CTAG 2 cut(s) 56, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.