Rmu_sc0003481.1_g000002
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003481.1
OG0020712
Physical Location & Seq
Forward (+)
2199 .. 6802
4604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003481.1_g000002.1.cds

Sequence Viewer

Length: 534 bp
atgcatgagatctgcagcctcgtcgctgacgacggcttcaaaatcgatgtaggccatggcttgatggccggagctccagtctgcgctcaaaggttccagttcgaagttcgtcgaccttttcactgcaggaaggttccagttcgaaccggtgacccgattttggcgaccggtgaccagaatcaggcgaaagttggctggattccagctaccgaaattatagtgaatgatcaggacaacaggaccactccatctctcgatgctttcactgatactgagtgcttcattggtgatgcgacatttaatcagatcatcaggaaccccaccaactccatctttgtgaaaaggtcgacgaacttcgaactggaacctttgagcgcagactggagctccggccatcaagccatggcctacatcgattttgaagccgtcgtcagcgacgaggctgcagatctcatgcatggattggtccacgacggcgggggaagtggagtggaggcactagcaattggcatctctgctgtgttcggagtttag

Protein Analysis

177

Amino Acids

19.11

Weight (kDa)

4.68

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020712)

Species Orthologous Gene IDs
rosa_laevigata RLG00000032864
rosa_multiflora Rmu_sc0003481.1_g000002
rosa_samantha Rh4AG185600 Rh5BG085700 Rh7AG234000 Rh7AG254200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 112, 347
AciI CCGC 1 cut(s) 477
AcoI YGGCCR 2 cut(s) 66, 391
AfiI CCNNNNNNNGG 2 cut(s) 160, 181
AgeI ACCGGT 2 cut(s) 146, 167
AgsI TTSAA 2 cut(s) 40, 422
AluBI AGCT 3 cut(s) 74, 206, 387
AluI AGCT 3 cut(s) 74, 206, 387
Alw21I GWGCWC 2 cut(s) 76, 389
AoxI GGCC 4 cut(s) 52, 66, 391, 405
ApeKI GCWGC 2 cut(s) 15, 443
AsiGI ACCGGT 2 cut(s) 146, 167
AspLEI GCGC 2 cut(s) 86, 377
AspS9I GGNCC 2 cut(s) 240, 466
AsuHPI GGTGA 3 cut(s) 161, 182, 299
AsuII TTCGAA 3 cut(s) 102, 142, 357
AvaII GGWCC 2 cut(s) 240, 466
BanII GRGCYC 2 cut(s) 76, 389
Bbv12I GWGCWC 2 cut(s) 76, 389
BbvI GCAGC 2 cut(s) 27, 430
BccI CCATC 4 cut(s) 58, 256, 338, 402
BceAI ACGGC 3 cut(s) 49, 410, 490
BcgI CGANNNNNNTGC 4 cut(s) 4, 38, 425, 459
BclI TGATCA 1 cut(s) 226
BfaI CTAG 1 cut(s) 500
BfmI CTRYAG 3 cut(s) 13, 124, 444
BglII AGATCT 2 cut(s) 9, 448
BisI GCNGC 2 cut(s) 16, 444
BlsI GCNGC 2 cut(s) 17, 445
Bme18I GGWCC 2 cut(s) 240, 466
BmgT120I GGNCC 2 cut(s) 240, 466
BmiI GGNNCC 4 cut(s) 95, 135, 317, 366
BmsI GCATC 3 cut(s) 247, 280, 519
BpmI CTGGAG 2 cut(s) 60, 403
Bpu14I TTCGAA 3 cut(s) 102, 142, 357
Bsa29I ATCGAT 2 cut(s) 45, 414
BsaJI CCNNGG 2 cut(s) 55, 402
BsaWI WCCGGW 2 cut(s) 146, 167
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 371, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 181
Bse118I RCCGGY 2 cut(s) 146, 167
Bse1I ACTGG 5 cut(s) 77, 97, 137, 366, 386
BseCI ATCGAT 2 cut(s) 45, 414
BseDI CCNNGG 2 cut(s) 55, 402
BseLI CCNNNNNNNGG 2 cut(s) 160, 181
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 5 cut(s) 77, 97, 137, 366, 386
BseXI GCAGC 2 cut(s) 27, 430
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 4 cut(s) 54, 68, 393, 407
BshTI ACCGGT 2 cut(s) 146, 167
BshVI ATCGAT 2 cut(s) 45, 414
BsiEI CGRYCG 1 cut(s) 168
BsiHKAI GWGCWC 2 cut(s) 76, 389
BsiSI CCGG 4 cut(s) 69, 147, 168, 390
BslI CCNNNNNNNGG 2 cut(s) 160, 181
BsnI GGCC 4 cut(s) 54, 68, 393, 407
Bsp119I TTCGAA 3 cut(s) 102, 142, 357
Bsp1286I GDGCHC 2 cut(s) 76, 389
Bsp143I GATC 4 cut(s) 9, 226, 306, 448
Bsp19I CCATGG 2 cut(s) 55, 402
BspACI CCGC 1 cut(s) 477
BspANI GGCC 4 cut(s) 54, 68, 393, 407
BspCNI CTCAG 1 cut(s) 265
BspDI ATCGAT 2 cut(s) 45, 414
BspLI GGNNCC 4 cut(s) 95, 135, 317, 366
BspMAI CTGCAG 3 cut(s) 17, 128, 448
BspT104I TTCGAA 3 cut(s) 102, 142, 357
BsrFI RCCGGY 2 cut(s) 146, 167
BsrI ACTGG 5 cut(s) 77, 97, 137, 366, 386
BssAI RCCGGY 2 cut(s) 146, 167
BssECI CCNNGG 2 cut(s) 55, 402
BssMI GATC 4 cut(s) 9, 226, 306, 448
BssT1I CCWWGG 2 cut(s) 55, 402
BstBI TTCGAA 3 cut(s) 102, 142, 357
BstDEI CTNAG 1 cut(s) 273
BstDSI CCRYGG 2 cut(s) 55, 402
BstEII GGTNACC 2 cut(s) 149, 170
BstHHI GCGC 2 cut(s) 86, 377
BstKTI GATC 4 cut(s) 12, 229, 309, 451
BstMBI GATC 4 cut(s) 9, 226, 306, 448
BstMCI CGRYCG 1 cut(s) 168
BstPI GGTNACC 2 cut(s) 149, 170
BstSFI CTRYAG 3 cut(s) 13, 124, 444
BstV1I GCAGC 2 cut(s) 27, 430
BstX2I RGATCY 2 cut(s) 9, 448
BstYI RGATCY 2 cut(s) 9, 448
Bsu15I ATCGAT 2 cut(s) 45, 414
BsuRI GGCC 4 cut(s) 54, 68, 393, 407
BsuTUI ATCGAT 2 cut(s) 45, 414
BtgI CCRYGG 2 cut(s) 55, 402
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 2 cut(s) 121, 264
CfoI GCGC 2 cut(s) 86, 377
Cfr10I RCCGGY 2 cut(s) 146, 167
Cfr13I GGNCC 2 cut(s) 240, 466
ClaI ATCGAT 2 cut(s) 45, 414
CspAI ACCGGT 2 cut(s) 146, 167
CviAII CATG 5 cut(s) 5, 56, 403, 454, 458
DdeI CTNAG 1 cut(s) 273
DpnI GATC 4 cut(s) 11, 228, 308, 450
DpnII GATC 4 cut(s) 9, 226, 306, 448
EaeI YGGCCR 2 cut(s) 66, 391
Ecl136II GAGCTC 2 cut(s) 74, 387
Eco130I CCWWGG 2 cut(s) 55, 402
Eco24I GRGCYC 2 cut(s) 76, 389
Eco47I GGWCC 2 cut(s) 240, 466
Eco53kI GAGCTC 2 cut(s) 74, 387
Eco91I GGTNACC 2 cut(s) 149, 170
EcoICRI GAGCTC 2 cut(s) 74, 387
EcoO65I GGTNACC 2 cut(s) 149, 170
EcoT14I CCWWGG 2 cut(s) 55, 402
EcoT22I ATGCAT 2 cut(s) 6, 459
EcoT38I GRGCYC 2 cut(s) 76, 389
ErhI CCWWGG 2 cut(s) 55, 402
FaeI CATG 5 cut(s) 8, 59, 406, 457, 461
FaiI YATR 6 cut(s) 6, 57, 218, 404, 455, 459
FatI CATG 5 cut(s) 4, 55, 402, 453, 457
FauI CCCGC 1 cut(s) 470
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 2 cut(s) 112, 347
Fnu4HI GCNGC 2 cut(s) 16, 444
FriOI GRGCYC 2 cut(s) 76, 389
Fsp4HI GCNGC 2 cut(s) 16, 444
FspBI CTAG 1 cut(s) 500
GlaI GCGC 2 cut(s) 85, 376
GluI GCNGC 2 cut(s) 16, 444
GsuI CTGGAG 2 cut(s) 60, 403
HaeIII GGCC 4 cut(s) 54, 68, 393, 407
HapII CCGG 4 cut(s) 69, 147, 168, 390
HhaI GCGC 2 cut(s) 86, 377
Hin1II CATG 5 cut(s) 8, 59, 406, 457, 461
Hin6I GCGC 2 cut(s) 84, 375
HinP1I GCGC 2 cut(s) 84, 375
HincII GTYRAC 2 cut(s) 113, 348
HindII GTYRAC 2 cut(s) 113, 348
HinfI GANTC 2 cut(s) 178, 199
HpaII CCGG 4 cut(s) 69, 147, 168, 390
HphI GGTGA 3 cut(s) 161, 182, 299
Hpy166II GTNNAC 3 cut(s) 113, 348, 469
Hpy188I TCNGA 2 cut(s) 306, 527
Hpy188III TCNNGA 3 cut(s) 230, 254, 313
Hpy8I GTNNAC 3 cut(s) 113, 348, 469
Hpy99I CGWCG 7 cut(s) 26, 35, 114, 352, 431, 440, 476
HpyAV CCTTC 1 cut(s) 124
HpyCH4V TGCA 5 cut(s) 4, 15, 126, 446, 457
HpyF3I CTNAG 1 cut(s) 273
Hsp92II CATG 5 cut(s) 8, 59, 406, 457, 461
HspAI GCGC 2 cut(s) 84, 375
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 4 cut(s) 9, 226, 306, 448
LmnI GCTCC 4 cut(s) 71, 79, 384, 392
Lsp1109I GCAGC 2 cut(s) 27, 430
LweI GCATC 3 cut(s) 247, 280, 519
MaeI CTAG 1 cut(s) 500
MaeIII GTNAC 2 cut(s) 149, 170
MalI GATC 4 cut(s) 11, 228, 308, 450
MboI GATC 4 cut(s) 9, 226, 306, 448
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 2 cut(s) 9, 448
MhlI GDGCHC 2 cut(s) 76, 389
MluCI AATT 2 cut(s) 213, 504
MnlI CCTC 3 cut(s) 29, 433, 487
Mph1103I ATGCAT 2 cut(s) 6, 459
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 335
MspI CCGG 4 cut(s) 69, 147, 168, 390
MunI CAATTG 1 cut(s) 504
NcoI CCATGG 2 cut(s) 55, 402
NdeII GATC 4 cut(s) 9, 226, 306, 448
NlaIII CATG 5 cut(s) 8, 59, 406, 457, 461
NlaIV GGNNCC 4 cut(s) 95, 135, 317, 366
NmuCI GTSAC 2 cut(s) 149, 170
NsiI ATGCAT 2 cut(s) 6, 459
NspV TTCGAA 3 cut(s) 102, 142, 357
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 435
PfeI GAWTC 2 cut(s) 178, 199
PinAI ACCGGT 2 cut(s) 146, 167
PkrI GCNGC 2 cut(s) 17, 445
Psp124BI GAGCTC 2 cut(s) 76, 389
PspEI GGTNACC 2 cut(s) 149, 170
PspN4I GGNNCC 4 cut(s) 95, 135, 317, 366
PspPI GGNCC 2 cut(s) 240, 466
PstI CTGCAG 3 cut(s) 17, 128, 448
PsuI RGATCY 2 cut(s) 9, 448
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 2 cut(s) 76, 389
SalI GTCGAC 2 cut(s) 111, 346
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 2 cut(s) 16, 444
Sau3AI GATC 4 cut(s) 9, 226, 306, 448
Sau96I GGNCC 2 cut(s) 240, 466
SduI GDGCHC 2 cut(s) 76, 389
SetI ASST 8 cut(s) 76, 95, 118, 135, 208, 347, 370, 389
SfaNI GCATC 3 cut(s) 247, 280, 519
SfcI CTRYAG 3 cut(s) 13, 124, 444
SfuI TTCGAA 3 cut(s) 102, 142, 357
SinI GGWCC 2 cut(s) 240, 466
SmiMI CAYNNNNRTG 1 cut(s) 335
Sse9I AATT 2 cut(s) 213, 504
SsiI CCGC 1 cut(s) 477
SspMI CTAG 1 cut(s) 500
SstI GAGCTC 2 cut(s) 76, 389
StyI CCWWGG 2 cut(s) 55, 402
TaqI TCGA 8 cut(s) 45, 102, 112, 142, 255, 347, 357, 414
TasI AATT 2 cut(s) 213, 504
TfiI GAWTC 2 cut(s) 178, 199
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 128, 271
TseFI GTSAC 2 cut(s) 149, 170
TseI GCWGC 2 cut(s) 15, 443
Tsp45I GTSAC 2 cut(s) 149, 170
TspDTI ATGAA 1 cut(s) 271
TspRI CASTG 2 cut(s) 128, 271
VpaK11BI GGWCC 2 cut(s) 240, 466
XmiI GTMKAC 2 cut(s) 112, 347
XspI CTAG 1 cut(s) 500
Zsp2I ATGCAT 2 cut(s) 6, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.