Rh4AG185600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0020712
Physical Location & Seq
Reverse (-)
46178300 .. 46184939
6640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG185600.1

Sequence Viewer

Length: 375 bp
ATGACGTCGATGGAGGTGATTCTCAGTGAATACCCAAATCCGGCGCCATCGATTGTCGCCGATGTTGGTGGATCGGCGATCACGCGACCTGAACACGATGGAGGCAGCAGCGAGCGCAACAAAGATGACGACGACGACGCAGAAGATGACTTGATCAAACCAGGCTCAAACCCTGCAATGAAAAGGTCGACGAACTTCGAACTGGAACCTTTGAGCGTGGACTGGAGCTCCGGCCATCAATCCACAGAGTACATCGATTTTGGAGCCGTCGTCGGAGATGGGGCTGCAGATCACGTTAACATATTGGTCGACAACGGCCAGGGAAGTGGAGTGGAGGCACTAGCAATTGGAGTCTCTGCCGTGTTCGGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

12.89

Weight (kDa)

4.05

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020712)

Species Orthologous Gene IDs
rosa_laevigata RLG00000032864
rosa_multiflora Rmu_sc0003481.1_g000002
rosa_samantha Rh4AG185600 Rh5BG085700 Rh7AG234000 Rh7AG254200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 43
AccI GTMKAC 2 cut(s) 188, 309
AccII CGCG 1 cut(s) 85
AclWI GGATC 1 cut(s) 79
AcoI YGGCCR 2 cut(s) 232, 316
AcyI GRCGYC 2 cut(s) 5, 44
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 40
AjnI CCWGG 2 cut(s) 160, 318
AluBI AGCT 1 cut(s) 228
AluI AGCT 1 cut(s) 228
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 1 cut(s) 79
AoxI GGCC 2 cut(s) 232, 316
ApeKI GCWGC 3 cut(s) 105, 108, 284
AspLEI GCGC 2 cut(s) 46, 117
AsuHPI GGTGA 1 cut(s) 28
AsuII TTCGAA 1 cut(s) 198
BanI GGYRCC 1 cut(s) 43
BanII GRGCYC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 3 cut(s) 117, 120, 271
BccI CCATC 5 cut(s) 4, 55, 92, 243, 272
BceAI ACGGC 3 cut(s) 251, 331, 344
BciT130I CCWGG 2 cut(s) 162, 320
BclI TGATCA 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 358
BfaI CTAG 1 cut(s) 341
BfmI CTRYAG 1 cut(s) 285
BfoI RGCGCY 1 cut(s) 47
BisI GCNGC 3 cut(s) 106, 109, 285
BlsI GCNGC 3 cut(s) 107, 110, 286
Bme1390I CCNGG 2 cut(s) 162, 320
BmiI GGNNCC 3 cut(s) 45, 207, 265
BmrFI CCNGG 2 cut(s) 162, 320
BpmI CTGGAG 1 cut(s) 244
Bpu14I TTCGAA 1 cut(s) 198
Bsa29I ATCGAT 2 cut(s) 50, 255
BsaHI GRCGYC 2 cut(s) 5, 44
BsaJI CCNNGG 1 cut(s) 319
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 207, 227
Bse3DI GCAATG 1 cut(s) 183
BseBI CCWGG 2 cut(s) 162, 320
BseCI ATCGAT 2 cut(s) 50, 255
BseDI CCNNGG 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 40
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 37
BseNI ACTGG 2 cut(s) 207, 227
BseXI GCAGC 3 cut(s) 117, 120, 271
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 2 cut(s) 234, 318
BshNI GGYRCC 1 cut(s) 43
BshVI ATCGAT 2 cut(s) 50, 255
BsiHKAI GWGCWC 1 cut(s) 230
BsiSI CCGG 2 cut(s) 41, 231
BslI CCNNNNNNNGG 1 cut(s) 40
BsmAI GTCTC 1 cut(s) 358
BsnI GGCC 2 cut(s) 234, 318
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 230
Bsp143I GATC 4 cut(s) 71, 78, 153, 289
BspANI GGCC 2 cut(s) 234, 318
BspCNI CTCAG 1 cut(s) 36
BspDI ATCGAT 2 cut(s) 50, 255
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 45, 207, 265
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 1 cut(s) 79
BspT104I TTCGAA 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 2 cut(s) 207, 227
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 4 cut(s) 71, 78, 153, 289
BssNI GRCGYC 2 cut(s) 5, 44
Bst2UI CCWGG 2 cut(s) 162, 320
BstACI GRCGYC 2 cut(s) 5, 44
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 113
BstDEI CTNAG 1 cut(s) 23
BstFNI CGCG 1 cut(s) 85
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 2 cut(s) 46, 117
BstKTI GATC 4 cut(s) 74, 81, 156, 292
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 4 cut(s) 71, 78, 153, 289
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 2 cut(s) 162, 320
BstSCI CCNGG 2 cut(s) 160, 318
BstSFI CTRYAG 1 cut(s) 285
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 3 cut(s) 117, 120, 271
BstXI CCANNNNNNTGG 1 cut(s) 326
Bsu15I ATCGAT 2 cut(s) 50, 255
BsuRI GGCC 2 cut(s) 234, 318
BsuTUI ATCGAT 2 cut(s) 50, 255
BtsIMutI CAGTG 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 113
CfoI GCGC 2 cut(s) 46, 117
ClaI ATCGAT 2 cut(s) 50, 255
CseI GACGC 1 cut(s) 146
Csp6I GTAC 1 cut(s) 250
CviJI RGCY 6 cut(s) 165, 228, 234, 266, 284, 318
CviKI_1 RGCY 6 cut(s) 165, 228, 234, 266, 284, 318
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 1 cut(s) 23
DinI GGCGCC 1 cut(s) 45
DpnI GATC 4 cut(s) 73, 80, 155, 291
DpnII GATC 4 cut(s) 71, 78, 153, 289
EaeI YGGCCR 2 cut(s) 232, 316
Ecl136II GAGCTC 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 230
Eco53kI GAGCTC 1 cut(s) 228
EcoICRI GAGCTC 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 160, 318
EcoT38I GRGCYC 1 cut(s) 230
EgeI GGCGCC 1 cut(s) 45
EheI GGCGCC 1 cut(s) 45
FaiI YATR 1 cut(s) 302
FbaI TGATCA 1 cut(s) 153
FblI GTMKAC 2 cut(s) 188, 309
Fnu4HI GCNGC 3 cut(s) 106, 109, 285
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 3 cut(s) 106, 109, 285
FspBI CTAG 1 cut(s) 341
GlaI GCGC 2 cut(s) 45, 116
GluI GCNGC 3 cut(s) 106, 109, 285
GsuI CTGGAG 1 cut(s) 244
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 2 cut(s) 234, 318
HapII CCGG 2 cut(s) 41, 231
HgaI GACGC 1 cut(s) 146
HhaI GCGC 2 cut(s) 46, 117
Hin1I GRCGYC 2 cut(s) 5, 44
Hin6I GCGC 2 cut(s) 44, 115
HinP1I GCGC 2 cut(s) 44, 115
HincII GTYRAC 3 cut(s) 189, 298, 310
HindII GTYRAC 3 cut(s) 189, 298, 310
HinfI GANTC 2 cut(s) 19, 351
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 2 cut(s) 41, 231
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 4 cut(s) 189, 220, 298, 310
Hpy188I TCNGA 2 cut(s) 275, 368
Hpy8I GTNNAC 4 cut(s) 189, 220, 298, 310
Hpy99I CGWCG 7 cut(s) 10, 134, 137, 140, 193, 272, 275
HpyCH4IV ACGT 2 cut(s) 5, 294
HpyCH4V TGCA 2 cut(s) 176, 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 1 cut(s) 23
HpySE526I ACGT 2 cut(s) 5, 294
Hsp92I GRCGYC 2 cut(s) 5, 44
HspAI GCGC 2 cut(s) 44, 115
KasI GGCGCC 1 cut(s) 43
Ksp22I TGATCA 1 cut(s) 153
KspAI GTTAAC 1 cut(s) 298
Kzo9I GATC 4 cut(s) 71, 78, 153, 289
LmnI GCTCC 3 cut(s) 225, 233, 263
Lsp1109I GCAGC 3 cut(s) 117, 120, 271
MaeI CTAG 1 cut(s) 341
MaeII ACGT 2 cut(s) 5, 294
MalI GATC 4 cut(s) 73, 80, 155, 291
MboI GATC 4 cut(s) 71, 78, 153, 289
MboII GAAGA 1 cut(s) 155
MfeI CAATTG 1 cut(s) 345
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 1 cut(s) 345
Mly113I GGCGCC 1 cut(s) 44
MlyI GAGTC 1 cut(s) 360
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 3 cut(s) 7, 95, 328
MseI TTAA 1 cut(s) 297
MspI CCGG 2 cut(s) 41, 231
MspR9I CCNGG 2 cut(s) 162, 320
MunI CAATTG 1 cut(s) 345
MvaI CCWGG 2 cut(s) 162, 320
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 1 cut(s) 114
NarI GGCGCC 1 cut(s) 44
NdeII GATC 4 cut(s) 71, 78, 153, 289
NlaIV GGNNCC 3 cut(s) 45, 207, 265
NspV TTCGAA 1 cut(s) 198
PfeI GAWTC 1 cut(s) 19
PkrI GCNGC 3 cut(s) 107, 110, 286
PleI GAGTC 1 cut(s) 359
PluTI GGCGCC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 359
Psp124BI GAGCTC 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 160, 318
PspGI CCWGG 2 cut(s) 160, 318
PspN4I GGNNCC 3 cut(s) 45, 207, 265
PstI CTGCAG 1 cut(s) 289
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SacI GAGCTC 1 cut(s) 230
SalI GTCGAC 2 cut(s) 187, 308
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 3 cut(s) 106, 109, 285
Sau3AI GATC 4 cut(s) 71, 78, 153, 289
SchI GAGTC 1 cut(s) 360
ScrFI CCNGG 2 cut(s) 162, 320
SduI GDGCHC 1 cut(s) 230
SetI ASST 7 cut(s) 8, 18, 91, 188, 211, 230, 297
SfcI CTRYAG 1 cut(s) 285
SfoI GGCGCC 1 cut(s) 45
SfuI TTCGAA 1 cut(s) 198
Sse9I AATT 1 cut(s) 345
SspDI GGCGCC 1 cut(s) 43
SspMI CTAG 1 cut(s) 341
SstI GAGCTC 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 160, 318
TaiI ACGT 2 cut(s) 8, 297
TaqI TCGA 6 cut(s) 8, 50, 188, 198, 255, 309
TasI AATT 1 cut(s) 345
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 1 cut(s) 31
TseI GCWGC 3 cut(s) 105, 108, 284
TspDTI ATGAA 1 cut(s) 194
TspRI CASTG 1 cut(s) 31
XmiI GTMKAC 2 cut(s) 188, 309
XspI CTAG 1 cut(s) 341
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.