Rh7AG234000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0020712
Physical Location & Seq
Reverse (-)
22717156 .. 22717404
249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG234000.1

Sequence Viewer

Length: 249 bp
ATGACGACGACGACGCAGAACATGACTCGATCAAACCAAGCTCAAACCCTGCTGGGTGAAAAGGTCGACGAACTTCGAATTGGAACCTTCGAGTGCGGACTGGAGCTCCATCAAGCCACAGAGTACATCGATTTTGGAGCCGTCGTCGGCGGCGAGGCTGCAGATCTCGCAAACGGATTGGTTGACGACGGCGGGGGAAGTGGAACGGAGGCACTAGCAATTGGCGTCTCTGCCATGTTCGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

82

Amino Acids

8.5

Weight (kDa)

4.05

Isoelectric Point (pI)

16.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020712)

Species Orthologous Gene IDs
rosa_laevigata RLG00000032864
rosa_multiflora Rmu_sc0003481.1_g000002
rosa_samantha Rh4AG185600 Rh5BG085700 Rh7AG234000 Rh7AG254200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 66
AciI CCGC 3 cut(s) 96, 150, 192
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 1 cut(s) 125
AjuI GAANNNNNNNTTGG 2 cut(s) 63, 95
AluBI AGCT 2 cut(s) 41, 106
AluI AGCT 2 cut(s) 41, 106
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 1 cut(s) 232
ApeKI GCWGC 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 68
AsuII TTCGAA 2 cut(s) 76, 240
BanII GRGCYC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 1 cut(s) 145
BccI CCATC 1 cut(s) 117
BceAI ACGGC 2 cut(s) 125, 205
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 159
BglII AGATCT 1 cut(s) 163
BisI GCNGC 2 cut(s) 151, 159
BlsI GCNGC 2 cut(s) 152, 160
BmiI GGNNCC 2 cut(s) 85, 139
BpmI CTGGAG 1 cut(s) 122
Bpu14I TTCGAA 2 cut(s) 76, 240
Bsa29I ATCGAT 1 cut(s) 129
BsaHI GRCGYC 1 cut(s) 225
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 129, 159
Bse1I ACTGG 1 cut(s) 105
BseCI ATCGAT 1 cut(s) 129
BseNI ACTGG 1 cut(s) 105
BseXI GCAGC 1 cut(s) 145
BseYI CCCAGC 1 cut(s) 52
BshVI ATCGAT 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 108
BsmAI GTCTC 1 cut(s) 232
BsmBI CGTCTC 1 cut(s) 232
Bsp119I TTCGAA 2 cut(s) 76, 240
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 2 cut(s) 29, 163
BspACI CCGC 3 cut(s) 96, 150, 192
BspDI ATCGAT 1 cut(s) 129
BspLI GGNNCC 2 cut(s) 85, 139
BspMAI CTGCAG 1 cut(s) 163
BspT104I TTCGAA 2 cut(s) 76, 240
BsrI ACTGG 1 cut(s) 105
BssMI GATC 2 cut(s) 29, 163
BssNI GRCGYC 1 cut(s) 225
BstACI GRCGYC 1 cut(s) 225
BstBI TTCGAA 2 cut(s) 76, 240
BstKTI GATC 2 cut(s) 32, 166
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 2 cut(s) 29, 163
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 145
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
Bsu15I ATCGAT 1 cut(s) 129
BsuTUI ATCGAT 1 cut(s) 129
ClaI ATCGAT 1 cut(s) 129
CseI GACGC 2 cut(s) 22, 214
Csp6I GTAC 1 cut(s) 124
CviAII CATG 2 cut(s) 22, 235
CviJI RGCY 5 cut(s) 41, 106, 116, 140, 158
CviKI_1 RGCY 5 cut(s) 41, 106, 116, 140, 158
CviQI GTAC 1 cut(s) 124
DpnI GATC 2 cut(s) 31, 165
DpnII GATC 2 cut(s) 29, 163
Ecl136II GAGCTC 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 108
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 2 cut(s) 25, 238
FaiI YATR 2 cut(s) 23, 236
FatI CATG 2 cut(s) 21, 234
FauI CCCGC 1 cut(s) 185
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 151, 159
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 2 cut(s) 151, 159
FspBI CTAG 1 cut(s) 215
GluI GCNGC 2 cut(s) 151, 159
GsaI CCCAGC 1 cut(s) 56
GsuI CTGGAG 1 cut(s) 122
HgaI GACGC 2 cut(s) 22, 214
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 2 cut(s) 25, 238
HincII GTYRAC 2 cut(s) 67, 184
HindII GTYRAC 2 cut(s) 67, 184
HinfI GANTC 1 cut(s) 25
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 2 cut(s) 67, 184
Hpy8I GTNNAC 2 cut(s) 67, 184
Hpy99I CGWCG 7 cut(s) 10, 13, 16, 71, 146, 149, 191
HpyAV CCTTC 1 cut(s) 97
HpyCH4V TGCA 1 cut(s) 161
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 2 cut(s) 25, 238
Kzo9I GATC 2 cut(s) 29, 163
LmnI GCTCC 3 cut(s) 103, 111, 137
LpnPI CCDG 3 cut(s) 38, 62, 86
Lsp1109I GCAGC 1 cut(s) 145
MaeI CTAG 1 cut(s) 215
MalI GATC 2 cut(s) 31, 165
MboI GATC 2 cut(s) 29, 163
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 2 cut(s) 78, 219
MlyI GAGTC 1 cut(s) 19
MnlI CCTC 2 cut(s) 148, 202
MunI CAATTG 1 cut(s) 219
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 2 cut(s) 29, 163
NlaIII CATG 2 cut(s) 25, 238
NlaIV GGNNCC 2 cut(s) 85, 139
NspV TTCGAA 2 cut(s) 76, 240
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PkrI GCNGC 2 cut(s) 152, 160
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Psp124BI GAGCTC 1 cut(s) 108
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 85, 139
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 1 cut(s) 163
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SacI GAGCTC 1 cut(s) 108
SalI GTCGAC 1 cut(s) 65
SatI GCNGC 2 cut(s) 151, 159
Sau3AI GATC 2 cut(s) 29, 163
SchI GAGTC 1 cut(s) 19
SduI GDGCHC 1 cut(s) 108
SetI ASST 4 cut(s) 43, 66, 89, 108
SfcI CTRYAG 1 cut(s) 159
SfuI TTCGAA 2 cut(s) 76, 240
Sse9I AATT 2 cut(s) 78, 219
SsiI CCGC 3 cut(s) 96, 150, 192
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 108
TaqI TCGA 6 cut(s) 28, 66, 76, 90, 129, 240
TasI AATT 2 cut(s) 78, 219
TatI WGTACW 1 cut(s) 123
TauI GCSGC 1 cut(s) 153
TseI GCWGC 1 cut(s) 158
TspGWI ACGGA 2 cut(s) 189, 221
XmiI GTMKAC 1 cut(s) 66
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.