Rh7AG254200

histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0020712
Physical Location & Seq
Reverse (-)
25490249 .. 25503795
13547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG254200.1

Sequence Viewer

Length: 684 bp
ATGGTGGAGGAGAGCGATTCCGGCGTCCTCGAGCAGCCAAAAATCGATCACGTCGTTGCCGTCCCTATCCCGATAATGGAGAAGAGCGATTTCGGCGTCATTGATATGGTTGTTGGGGAGCCTGCTACCCGCGGGCCTATCCCGATGTCGAACAGCAGTTCAGGAAGCAGGAGATCTAGATGGCTAGTACTAAGCAAATTGCTTGCCAGTCAACTAGAAGAAAGGCTCCAGGGAAGCATCTGGCTACCAAGTTGGCTCGAAAGTTTGCCCGGCCCCAACCACCACAGAGTTAAGAAGCCTCACAGAATTCGCCCTGGTACTGTTGCTCTTCGTCAAATCCCTAAGCACTACAATAGTACAGAGTTCCAGATACGTAAACTGCCATTCTTGTTCGCGAATATTGCCCACCGTAACTTCGCGAGTGGTGACTGTTTCCAGAGCAAAGAGTTGTTGGCATTGCAGGAGGCTGCGGAGGCGTATCTAGCTTTGAAAAGGTCGACAAACTTCGAACTGGAACCTTGGAGCGCGGACTGGAGCTCCGGCCATCAAGCCACGGCGTACATCGATTTTGGAGCCGTCGTCGGCGACGAGGCTGCAGATCTCTCGAATGGATTGGTCGACTACGGCGGGGGAAGTGGAGTGGAGGCACTAGCAATTGGCATCTCTGCTGTGTTCGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

227

Amino Acids

24.83

Weight (kDa)

5.65

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020712)

Species Orthologous Gene IDs
rosa_laevigata RLG00000032864
rosa_multiflora Rmu_sc0003481.1_g000002
rosa_samantha Rh4AG185600 Rh5BG085700 Rh7AG234000 Rh7AG254200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 497, 618
AccII CGCG 4 cut(s) 132, 395, 419, 527
AciI CCGC 5 cut(s) 130, 132, 470, 527, 627
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 1 cut(s) 306
AcyI GRCGYC 2 cut(s) 24, 96
AfaI GTAC 4 cut(s) 189, 319, 358, 560
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 1 cut(s) 490
AjiI CACGTC 1 cut(s) 52
AjnI CCWGG 2 cut(s) 228, 313
AluBI AGCT 2 cut(s) 485, 537
AluI AGCT 2 cut(s) 485, 537
Alw21I GWGCWC 1 cut(s) 539
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 3 cut(s) 134, 271, 541
ApeKI GCWGC 3 cut(s) 34, 467, 593
ApoI RAATTY 1 cut(s) 306
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 2 cut(s) 134, 272
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 437
AsuII TTCGAA 2 cut(s) 507, 675
AvaI CYCGRG 1 cut(s) 29
BanII GRGCYC 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 539
BbvI GCAGC 3 cut(s) 46, 454, 580
BccI CCATC 2 cut(s) 174, 552
BceAI ACGGC 4 cut(s) 44, 560, 570, 640
BcgI CGANNNNNNTGC 4 cut(s) 575, 585, 609, 619
BciT130I CCWGG 2 cut(s) 230, 315
BcnI CCSGG 1 cut(s) 270
BfaI CTAG 5 cut(s) 177, 185, 215, 482, 650
BfmI CTRYAG 1 cut(s) 594
BglII AGATCT 2 cut(s) 173, 598
BisI GCNGC 3 cut(s) 35, 468, 594
BlsI GCNGC 3 cut(s) 36, 469, 595
BmcAI AGTACT 1 cut(s) 189
Bme1390I CCNGG 3 cut(s) 230, 270, 315
BmeT110I CYCGRG 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 134, 272
BmiI GGNNCC 5 cut(s) 120, 227, 274, 516, 574
BmrFI CCNGG 3 cut(s) 230, 270, 315
BmsI GCATC 2 cut(s) 246, 669
BpmI CTGGAG 2 cut(s) 212, 553
Bpu10I CCTNAGC 1 cut(s) 342
Bpu14I TTCGAA 2 cut(s) 507, 675
BpuMI CCSGG 1 cut(s) 270
Bsa29I ATCGAT 2 cut(s) 45, 564
BsaAI YACGTR 1 cut(s) 374
BsaHI GRCGYC 2 cut(s) 24, 96
BsaJI CCNNGG 5 cut(s) 130, 229, 313, 518, 552
BsaXI ACNNNNNCTCC 4 cut(s) 521, 551, 564, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 3 cut(s) 207, 516, 536
Bse3DI GCAATG 1 cut(s) 455
BseBI CCWGG 2 cut(s) 230, 315
BseCI ATCGAT 2 cut(s) 45, 564
BseDI CCNNGG 5 cut(s) 130, 229, 313, 518, 552
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMI GCAATG 1 cut(s) 455
BseNI ACTGG 3 cut(s) 207, 516, 536
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 3 cut(s) 46, 454, 580
Bsh1236I CGCG 4 cut(s) 132, 395, 419, 527
BshFI GGCC 3 cut(s) 136, 273, 543
BshVI ATCGAT 2 cut(s) 45, 564
BsiHKAI GWGCWC 1 cut(s) 539
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 3 cut(s) 21, 270, 540
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 47
BsnI GGCC 3 cut(s) 136, 273, 543
BsoBI CYCGRG 1 cut(s) 29
Bsp119I TTCGAA 2 cut(s) 507, 675
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 3 cut(s) 46, 173, 598
Bsp68I TCGCGA 2 cut(s) 395, 419
BspACI CCGC 5 cut(s) 130, 132, 470, 527, 627
BspANI GGCC 3 cut(s) 136, 273, 543
BspDI ATCGAT 2 cut(s) 45, 564
BspFNI CGCG 4 cut(s) 132, 395, 419, 527
BspLI GGNNCC 5 cut(s) 120, 227, 274, 516, 574
BspMAI CTGCAG 1 cut(s) 598
BspQI GCTCTTC 2 cut(s) 77, 333
BspT104I TTCGAA 2 cut(s) 507, 675
BsrDI GCAATG 1 cut(s) 455
BsrI ACTGG 3 cut(s) 207, 516, 536
BssECI CCNNGG 5 cut(s) 130, 229, 313, 518, 552
BssMI GATC 3 cut(s) 46, 173, 598
BssNI GRCGYC 2 cut(s) 24, 96
BssT1I CCWWGG 1 cut(s) 518
Bst2UI CCWGG 2 cut(s) 230, 315
Bst4CI ACNGT 3 cut(s) 322, 410, 431
Bst6I CTCTTC 2 cut(s) 77, 333
BstACI GRCGYC 2 cut(s) 24, 96
BstBAI YACGTR 1 cut(s) 374
BstBI TTCGAA 2 cut(s) 507, 675
BstC8I GCNNGC 3 cut(s) 123, 134, 204
BstDEI CTNAG 2 cut(s) 191, 342
BstDSI CCRYGG 2 cut(s) 130, 552
BstFNI CGCG 4 cut(s) 132, 395, 419, 527
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 3 cut(s) 49, 176, 601
BstMBI GATC 3 cut(s) 46, 173, 598
BstMWI GCNNNNNNNGC 5 cut(s) 21, 93, 401, 473, 482
BstNI CCWGG 2 cut(s) 230, 315
BstSCI CCNGG 3 cut(s) 228, 268, 313
BstSFI CTRYAG 1 cut(s) 594
BstSNI TACGTA 1 cut(s) 374
BstUI CGCG 4 cut(s) 132, 395, 419, 527
BstV1I GCAGC 3 cut(s) 46, 454, 580
BstX2I RGATCY 2 cut(s) 173, 598
BstYI RGATCY 2 cut(s) 173, 598
Bsu15I ATCGAT 2 cut(s) 45, 564
BsuRI GGCC 3 cut(s) 136, 273, 543
BsuTUI ATCGAT 2 cut(s) 45, 564
BtgI CCRYGG 2 cut(s) 130, 552
BtrI CACGTC 1 cut(s) 52
BtuMI TCGCGA 2 cut(s) 395, 419
Cac8I GCNNGC 3 cut(s) 123, 134, 204
CfoI GCGC 1 cut(s) 527
Cfr13I GGNCC 2 cut(s) 134, 272
Cfr42I CCGCGG 1 cut(s) 133
ClaI ATCGAT 2 cut(s) 45, 564
CseI GACGC 2 cut(s) 13, 85
Csp6I GTAC 4 cut(s) 188, 318, 357, 559
CviQI GTAC 4 cut(s) 188, 318, 357, 559
DdeI CTNAG 2 cut(s) 191, 342
DpnI GATC 3 cut(s) 48, 175, 600
DpnII GATC 3 cut(s) 46, 173, 598
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 2 cut(s) 77, 333
EarI CTCTTC 2 cut(s) 77, 333
Ecl136II GAGCTC 1 cut(s) 537
Eco105I TACGTA 1 cut(s) 374
Eco130I CCWWGG 1 cut(s) 518
Eco24I GRGCYC 1 cut(s) 539
Eco53kI GAGCTC 1 cut(s) 537
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 537
EcoRI GAATTC 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 228, 313
EcoT14I CCWWGG 1 cut(s) 518
EcoT38I GRGCYC 1 cut(s) 539
ErhI CCWWGG 1 cut(s) 518
FaiI YATR 1 cut(s) 107
FaqI GGGAC 1 cut(s) 47
FauI CCCGC 3 cut(s) 125, 137, 620
FblI GTMKAC 2 cut(s) 497, 618
Fnu4HI GCNGC 3 cut(s) 35, 468, 594
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 3 cut(s) 35, 468, 594
FspBI CTAG 5 cut(s) 177, 185, 215, 482, 650
GlaI GCGC 1 cut(s) 526
GluI GCNGC 3 cut(s) 35, 468, 594
GsuI CTGGAG 2 cut(s) 212, 553
HaeIII GGCC 3 cut(s) 136, 273, 543
HapII CCGG 3 cut(s) 21, 270, 540
HgaI GACGC 2 cut(s) 13, 85
HhaI GCGC 1 cut(s) 527
Hin1I GRCGYC 2 cut(s) 24, 96
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HincII GTYRAC 3 cut(s) 212, 498, 619
HindII GTYRAC 3 cut(s) 212, 498, 619
HinfI GANTC 1 cut(s) 17
HpaII CCGG 3 cut(s) 21, 270, 540
HphI GGTGA 1 cut(s) 437
Hpy166II GTNNAC 4 cut(s) 212, 377, 498, 619
Hpy188III TCNNGA 9 cut(s) 70, 142, 162, 177, 367, 394, 418, 436, 604
Hpy8I GTNNAC 4 cut(s) 212, 377, 498, 619
Hpy99I CGWCG 4 cut(s) 56, 581, 584, 590
HpyCH4III ACNGT 3 cut(s) 322, 410, 431
HpyCH4IV ACGT 2 cut(s) 51, 373
HpyCH4V TGCA 2 cut(s) 460, 596
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 93, 401, 473, 482
HpyF3I CTNAG 2 cut(s) 191, 342
HpySE526I ACGT 2 cut(s) 51, 373
Hsp92I GRCGYC 2 cut(s) 24, 96
HspAI GCGC 1 cut(s) 525
KspI CCGCGG 1 cut(s) 133
Kzo9I GATC 3 cut(s) 46, 173, 598
LguI GCTCTTC 2 cut(s) 77, 333
LmnI GCTCC 6 cut(s) 118, 231, 522, 534, 542, 572
Lsp1109I GCAGC 3 cut(s) 46, 454, 580
LweI GCATC 2 cut(s) 246, 669
MaeI CTAG 5 cut(s) 177, 185, 215, 482, 650
MaeII ACGT 2 cut(s) 51, 373
MaeIII GTNAC 2 cut(s) 410, 425
MalI GATC 3 cut(s) 48, 175, 600
MboI GATC 3 cut(s) 46, 173, 598
MboII GAAGA 3 cut(s) 94, 230, 320
MfeI CAATTG 1 cut(s) 654
MflI RGATCY 2 cut(s) 173, 598
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 3 cut(s) 197, 306, 654
MnlI CCTC 6 cut(s) 38, 309, 457, 466, 583, 637
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 3 cut(s) 21, 270, 540
MspR9I CCNGG 3 cut(s) 230, 270, 315
MunI CAATTG 1 cut(s) 654
MvaI CCWGG 2 cut(s) 230, 315
MvnI CGCG 4 cut(s) 132, 395, 419, 527
MwoI GCNNNNNNNGC 5 cut(s) 21, 93, 401, 473, 482
NciI CCSGG 1 cut(s) 270
NdeII GATC 3 cut(s) 46, 173, 598
NlaIV GGNNCC 5 cut(s) 120, 227, 274, 516, 574
NmuCI GTSAC 1 cut(s) 425
NruI TCGCGA 2 cut(s) 395, 419
NspV TTCGAA 2 cut(s) 507, 675
PaeR7I CTCGAG 1 cut(s) 29
PciSI GCTCTTC 2 cut(s) 77, 333
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 57, 585
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 3 cut(s) 36, 469, 595
Ppu21I YACGTR 1 cut(s) 374
Psp124BI GAGCTC 1 cut(s) 539
Psp6I CCWGG 2 cut(s) 228, 313
PspGI CCWGG 2 cut(s) 228, 313
PspN4I GGNNCC 5 cut(s) 120, 227, 274, 516, 574
PspPI GGNCC 2 cut(s) 134, 272
PspXI VCTCGAGB 1 cut(s) 29
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 2 cut(s) 173, 598
RruI TCGCGA 2 cut(s) 395, 419
RsaI GTAC 4 cut(s) 189, 319, 358, 560
RsaNI GTAC 4 cut(s) 188, 318, 357, 559
RseI CAYNNNNRTG 1 cut(s) 104
SacI GAGCTC 1 cut(s) 539
SacII CCGCGG 1 cut(s) 133
SalI GTCGAC 2 cut(s) 496, 617
SapI GCTCTTC 2 cut(s) 77, 333
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 3 cut(s) 35, 468, 594
Sau3AI GATC 3 cut(s) 46, 173, 598
Sau96I GGNCC 2 cut(s) 134, 272
ScaI AGTACT 1 cut(s) 189
ScrFI CCNGG 3 cut(s) 230, 270, 315
SduI GDGCHC 1 cut(s) 539
SetI ASST 6 cut(s) 54, 376, 487, 497, 520, 539
SfaNI GCATC 2 cut(s) 246, 669
SfcI CTRYAG 1 cut(s) 594
Sfr274I CTCGAG 1 cut(s) 29
Sfr303I CCGCGG 1 cut(s) 133
SfuI TTCGAA 2 cut(s) 507, 675
SgrBI CCGCGG 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 29
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SnaBI TACGTA 1 cut(s) 374
Sse9I AATT 3 cut(s) 197, 306, 654
SsiI CCGC 5 cut(s) 130, 132, 470, 527, 627
SspI AATATT 1 cut(s) 400
SspMI CTAG 5 cut(s) 177, 185, 215, 482, 650
SstI GAGCTC 1 cut(s) 539
StyD4I CCNGG 3 cut(s) 228, 268, 313
StyI CCWWGG 1 cut(s) 518
TaaI ACNGT 3 cut(s) 322, 410, 431
TaiI ACGT 2 cut(s) 54, 376
TasI AATT 3 cut(s) 197, 306, 654
TatI WGTACW 2 cut(s) 187, 356
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 3 cut(s) 34, 467, 593
Tsp45I GTSAC 1 cut(s) 425
XapI RAATTY 1 cut(s) 306
XbaI TCTAGA 1 cut(s) 176
XhoI CTCGAG 1 cut(s) 29
XmiI GTMKAC 2 cut(s) 497, 618
XspI CTAG 5 cut(s) 177, 185, 215, 482, 650
ZrmI AGTACT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.