Rmu_sc0003722.1_g000040

Photosystem I reaction center subunit II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003722.1
OG0014442
Physical Location & Seq
Reverse (-)
192509 .. 195935
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1083 bp
atggcaactcaagcgaccctcttcaccccaaccgcctcagcaccatggaaacagtcagtcacctccaccttcatggccccaaagtcactgaccctctccaccgcagcgccaagacccatgaggatcgcagctgctgaagagaaaaccgtggcacccacaaaggaggccgaagctccggttggcttcaccccgccggaactggacccaacaaccccatccccaatcttcggcggcagcaccggtgggcttctccgcaaggcccaagaggaagagttctacgtgatcacctgggaatcacccaaggagcaaatctttgagatgccaactggtggtgcagccatcatgagggaaggacctaacttgttgaagctggcgaggaaagagcagtgtttggctcttgggactagactgaggtccaagtacaagatcaagtaccagttttacagggtgttccctaatggtgaggttcagtatttgcacccaaaagatggagtgtaccctgagaaggtgaacccagggcgccaaggagtggggcagaacttcaggtccattgggaagaatgtgaacccaattgaggtcaagttcactgggaagcaagtgattgagtcggcatctcgacctcctcctggagatgctggtgaggtgttctggttgggttatgcacttatgcttgctcggcccatcgcaaaaaccctaattgatttgctgacacagaagttggggttcaaagacaatatttgtttccaacatcaaagggatgctcaaggagtagcgctaatatggaatgaagacactaatgctgaatttagatcatcatctgcgcatcatatcgatgtagtcattggaaaactagaagatgcaacgaagtggaggtttactggtatttatggttttgcagcaagggaggctcgaacacaaagatgggatctgattttgtctcttgctgcgcagccatgcaccctgccttggcttatggcgggggacttcaacgagattatgtcactagcggacaagtctggaagccaaaggagagcgtccctgccaatggagactttccgaagaactatgacagcttgtggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PsaD

Protein Analysis

360

Amino Acids

39.87

Weight (kDa)

9.24

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaD PF02531 71 - 199 6.6e-68 PsaD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014442)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03130 AT4G02770
fragaria_vesca FvH4_4g15260
malus_domestica MD13G1191300.v1.1 MD16G1191400.v1.1
prunus_persica Prupe.1G011500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0416571
rosa_laevigata RLG00000007973
rosa_multiflora Rmu_sc0003722.1_g000040
rosa_roxburghii Rroxscaffold_5G00360450
rosa_rugosa Rorug04G0143000
rosa_samantha Rh4AG205400 Rh4BG203000 Rh4CG216300 Rh4DG202500
rosa_wichuraiana Rw0G013510 Rw4G017390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 822, 948
AccB1I GGYRCC 2 cut(s) 151, 519
AccB7I CCANNNNNTGG 3 cut(s) 329, 488, 529
AciI CCGC 7 cut(s) 33, 102, 191, 231, 253, 977, 1007
AclWI GGATC 2 cut(s) 131, 933
AcsI RAATTY 1 cut(s) 803
AcuI CTGAAG 2 cut(s) 156, 526
AcyI GRCGYC 1 cut(s) 520
AfaI GTAC 3 cut(s) 422, 434, 497
AfeI AGCGCT 1 cut(s) 774
AgeI ACCGGT 1 cut(s) 239
AgsI TTSAA 3 cut(s) 367, 727, 988
AjnI CCWGG 3 cut(s) 287, 514, 625
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AloI GAACNNNNNNTCC 2 cut(s) 530, 562
AluBI AGCT 4 cut(s) 131, 173, 370, 1073
AluI AGCT 4 cut(s) 131, 173, 370, 1073
Alw26I GTCTC 2 cut(s) 942, 1043
AlwI GGATC 2 cut(s) 131, 933
AlwNI CAGNNNCTG 1 cut(s) 134
Aor51HI AGCGCT 1 cut(s) 774
AoxI GGCC 4 cut(s) 75, 165, 258, 677
ApeKI GCWGC 8 cut(s) 104, 128, 131, 234, 335, 896, 944, 949
ApoI RAATTY 1 cut(s) 803
AsiGI ACCGGT 1 cut(s) 239
AspLEI GCGC 5 cut(s) 109, 522, 775, 823, 949
AspS9I GGNCC 7 cut(s) 76, 202, 259, 353, 414, 546, 678
AsuHPI GGTGA 8 cut(s) 16, 52, 178, 277, 288, 473, 520, 650
AvaII GGWCC 4 cut(s) 202, 353, 414, 546
BanI GGYRCC 2 cut(s) 151, 519
BbsI GAAGAC 1 cut(s) 795
BbvCI CCTCAGC 1 cut(s) 37
BbvI GCAGC 8 cut(s) 116, 118, 140, 246, 347, 908, 931, 961
BccI CCATC 5 cut(s) 223, 347, 482, 689, 915
BciT130I CCWGG 3 cut(s) 289, 516, 627
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 942, 1043
BfaI CTAG 3 cut(s) 405, 851, 1004
BfoI RGCGCY 3 cut(s) 110, 523, 776
BisI GCNGC 9 cut(s) 105, 129, 132, 232, 235, 336, 897, 945, 950
BlsI GCNGC 9 cut(s) 106, 130, 133, 233, 236, 337, 898, 946, 951
Bme1390I CCNGG 3 cut(s) 289, 516, 627
Bme18I GGWCC 4 cut(s) 202, 353, 414, 546
BmgT120I GGNCC 7 cut(s) 76, 202, 259, 353, 414, 546, 678
BmiI GGNNCC 4 cut(s) 78, 153, 204, 521
BmrFI CCNGG 3 cut(s) 289, 516, 627
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 6 cut(s) 309, 620, 622, 748, 832, 847
BmuI ACTGGG 1 cut(s) 597
BoxI GACNNNNGTC 1 cut(s) 412
BpiI GAAGAC 1 cut(s) 795
BpmI CTGGAG 1 cut(s) 648
Bpu10I CCTNAGC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 747
Bsa29I ATCGAT 1 cut(s) 831
BsaAI YACGTR 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 814
BsaHI GRCGYC 1 cut(s) 520
BsaJI CCNNGG 8 cut(s) 44, 147, 288, 300, 514, 515, 523, 965
BsaWI WCCGGW 2 cut(s) 175, 239
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 5 cut(s) 204, 331, 436, 592, 883
Bse8I GATNNNNATC 1 cut(s) 814
BseBI CCWGG 3 cut(s) 289, 516, 627
BseCI ATCGAT 1 cut(s) 831
BseDI CCNNGG 8 cut(s) 44, 147, 288, 300, 514, 515, 523, 965
BseGI GGATG 2 cut(s) 215, 763
BseJI GATNNNNATC 1 cut(s) 814
BseMII CTCAG 3 cut(s) 51, 401, 492
BseNI ACTGG 5 cut(s) 204, 331, 436, 592, 883
BseRI GAGGAG 1 cut(s) 612
BseXI GCAGC 8 cut(s) 116, 118, 140, 246, 347, 908, 931, 961
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 4 cut(s) 77, 167, 260, 679
BshNI GGYRCC 2 cut(s) 151, 519
BshTI ACCGGT 1 cut(s) 239
BshVI ATCGAT 1 cut(s) 831
BsiSI CCGG 3 cut(s) 176, 194, 240
BslFI GGGAC 3 cut(s) 415, 995, 1021
BsmAI GTCTC 2 cut(s) 942, 1043
BsmFI GGGAC 3 cut(s) 415, 995, 1021
BsnI GGCC 4 cut(s) 77, 167, 260, 679
Bsp143I GATC 5 cut(s) 123, 282, 426, 809, 925
Bsp19I CCATGG 1 cut(s) 44
BspACI CCGC 7 cut(s) 33, 102, 191, 231, 253, 977, 1007
BspANI GGCC 4 cut(s) 77, 167, 260, 679
BspCNI CTCAG 3 cut(s) 50, 402, 493
BspDI ATCGAT 1 cut(s) 831
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 4 cut(s) 78, 153, 204, 521
BspPI GGATC 2 cut(s) 131, 933
BspT107I GGYRCC 2 cut(s) 151, 519
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 5 cut(s) 204, 331, 436, 592, 883
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 8 cut(s) 44, 147, 288, 300, 514, 515, 523, 965
BssMI GATC 5 cut(s) 123, 282, 426, 809, 925
BssNI GRCGYC 1 cut(s) 520
BssT1I CCWWGG 4 cut(s) 44, 300, 523, 965
Bst2UI CCWGG 3 cut(s) 289, 516, 627
Bst4CI ACNGT 2 cut(s) 54, 148
Bst6I CTCTTC 3 cut(s) 26, 132, 264
BstACI GRCGYC 1 cut(s) 520
BstBAI YACGTR 1 cut(s) 280
BstC8I GCNNGC 2 cut(s) 372, 672
BstDEI CTNAG 3 cut(s) 37, 410, 501
BstDSI CCRYGG 2 cut(s) 44, 147
BstF5I GGATG 2 cut(s) 215, 763
BstH2I RGCGCY 3 cut(s) 110, 523, 776
BstHHI GCGC 5 cut(s) 109, 522, 775, 823, 949
BstKTI GATC 5 cut(s) 126, 285, 429, 812, 928
BstMAI GTCTC 2 cut(s) 942, 1043
BstMBI GATC 5 cut(s) 123, 282, 426, 809, 925
BstMWI GCNNNNNNNGC 3 cut(s) 11, 676, 905
BstNI CCWGG 3 cut(s) 289, 516, 627
BstPAI GACNNNNGTC 1 cut(s) 412
BstSCI CCNGG 3 cut(s) 287, 514, 625
BstV1I GCAGC 8 cut(s) 116, 118, 140, 246, 347, 908, 931, 961
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 1 cut(s) 925
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 1 cut(s) 925
Bsu15I ATCGAT 1 cut(s) 831
BsuRI GGCC 4 cut(s) 77, 167, 260, 679
BsuTUI ATCGAT 1 cut(s) 831
BtgI CCRYGG 2 cut(s) 44, 147
BtgZI GCGATG 1 cut(s) 667
BtsCI GGATG 2 cut(s) 215, 763
BtsI GCAGTG 1 cut(s) 392
BtsIMutI CAGTG 3 cut(s) 86, 392, 585
Cac8I GCNNGC 2 cut(s) 372, 672
CaiI CAGNNNCTG 1 cut(s) 134
CciI TCATGA 1 cut(s) 342
CfoI GCGC 5 cut(s) 109, 522, 775, 823, 949
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 7 cut(s) 76, 202, 259, 353, 414, 546, 678
ClaI ATCGAT 1 cut(s) 831
CseI GACGC 1 cut(s) 1023
Csp6I GTAC 3 cut(s) 421, 433, 496
CspAI ACCGGT 1 cut(s) 239
CviAII CATG 5 cut(s) 45, 73, 118, 343, 954
CviQI GTAC 3 cut(s) 421, 433, 496
DdeI CTNAG 3 cut(s) 37, 410, 501
DinI GGCGCC 1 cut(s) 521
DpnI GATC 5 cut(s) 125, 284, 428, 811, 927
DpnII GATC 5 cut(s) 123, 282, 426, 809, 925
Eam1104I CTCTTC 3 cut(s) 26, 132, 264
EarI CTCTTC 3 cut(s) 26, 132, 264
Eco130I CCWWGG 4 cut(s) 44, 300, 523, 965
Eco47I GGWCC 4 cut(s) 202, 353, 414, 546
Eco47III AGCGCT 1 cut(s) 774
Eco57I CTGAAG 2 cut(s) 156, 526
EcoO109I RGGNCCY 1 cut(s) 353
EcoRII CCWGG 3 cut(s) 287, 514, 625
EcoT14I CCWWGG 4 cut(s) 44, 300, 523, 965
EgeI GGCGCC 1 cut(s) 521
EheI GGCGCC 1 cut(s) 521
ErhI CCWWGG 4 cut(s) 44, 300, 523, 965
FaeI CATG 5 cut(s) 48, 76, 121, 346, 957
FaqI GGGAC 3 cut(s) 415, 995, 1021
FatI CATG 5 cut(s) 44, 72, 117, 342, 953
FauI CCCGC 2 cut(s) 198, 970
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 9 cut(s) 105, 129, 132, 232, 235, 336, 897, 945, 950
FokI GGATG 2 cut(s) 202, 770
Fsp4HI GCNGC 9 cut(s) 105, 129, 132, 232, 235, 336, 897, 945, 950
FspBI CTAG 3 cut(s) 405, 851, 1004
FspI TGCGCA 2 cut(s) 822, 948
GlaI GCGC 5 cut(s) 108, 521, 774, 822, 948
GluI GCNGC 9 cut(s) 105, 129, 132, 232, 235, 336, 897, 945, 950
GsuI CTGGAG 1 cut(s) 648
HaeII RGCGCY 3 cut(s) 110, 523, 776
HaeIII GGCC 4 cut(s) 77, 167, 260, 679
HapII CCGG 3 cut(s) 176, 194, 240
HgaI GACGC 1 cut(s) 1023
HhaI GCGC 5 cut(s) 109, 522, 775, 823, 949
Hin1I GRCGYC 1 cut(s) 520
Hin1II CATG 5 cut(s) 48, 76, 121, 346, 957
Hin6I GCGC 5 cut(s) 107, 520, 773, 821, 947
HinP1I GCGC 5 cut(s) 107, 520, 773, 821, 947
HinfI GANTC 2 cut(s) 293, 605
HpaII CCGG 3 cut(s) 176, 194, 240
HphI GGTGA 8 cut(s) 16, 52, 178, 277, 288, 473, 520, 650
Hpy166II GTNNAC 5 cut(s) 496, 511, 565, 585, 876
Hpy188I TCNGA 2 cut(s) 930, 1058
Hpy188III TCNNGA 3 cut(s) 343, 615, 1017
Hpy8I GTNNAC 5 cut(s) 496, 511, 565, 585, 876
HpyAV CCTTC 3 cut(s) 79, 344, 499
HpyCH4III ACNGT 2 cut(s) 54, 148
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 6 cut(s) 335, 478, 662, 860, 896, 957
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 676, 905
HpyF3I CTNAG 3 cut(s) 37, 410, 501
HpySE526I ACGT 1 cut(s) 279
Hsp92I GRCGYC 1 cut(s) 520
Hsp92II CATG 5 cut(s) 48, 76, 121, 346, 957
HspAI GCGC 5 cut(s) 107, 520, 773, 821, 947
KasI GGCGCC 1 cut(s) 519
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 5 cut(s) 123, 282, 426, 809, 925
LmnI GCTCC 2 cut(s) 178, 304
Lsp1109I GCAGC 8 cut(s) 116, 118, 140, 246, 347, 908, 931, 961
LweI GCATC 6 cut(s) 309, 620, 622, 748, 832, 847
MaeI CTAG 3 cut(s) 405, 851, 1004
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 3 cut(s) 58, 84, 999
MalI GATC 5 cut(s) 125, 284, 428, 811, 927
MboI GATC 5 cut(s) 123, 282, 426, 809, 925
MboII GAAGA 8 cut(s) 13, 149, 217, 281, 568, 800, 866, 1071
MfeI CAATTG 1 cut(s) 570
MflI RGATCY 1 cut(s) 925
MluCI AATT 3 cut(s) 570, 696, 803
Mly113I GGCGCC 1 cut(s) 520
MlyI GAGTC 1 cut(s) 614
MmeI TCCRAC 1 cut(s) 769
MseI TTAA 1 cut(s) 1081
MslI CAYNNNNRTG 3 cut(s) 71, 831, 919
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 3 cut(s) 176, 194, 240
MspR9I CCNGG 3 cut(s) 289, 516, 627
MunI CAATTG 1 cut(s) 570
MvaI CCWGG 3 cut(s) 289, 516, 627
MwoI GCNNNNNNNGC 3 cut(s) 11, 676, 905
NarI GGCGCC 1 cut(s) 520
NcoI CCATGG 1 cut(s) 44
NdeII GATC 5 cut(s) 123, 282, 426, 809, 925
NlaIII CATG 5 cut(s) 48, 76, 121, 346, 957
NlaIV GGNNCC 4 cut(s) 78, 153, 204, 521
NmeAIII GCCGAG 1 cut(s) 655
NmuCI GTSAC 3 cut(s) 58, 84, 999
NsbI TGCGCA 2 cut(s) 822, 948
PagI TCATGA 1 cut(s) 342
PasI CCCWGGG 1 cut(s) 515
PfeI GAWTC 1 cut(s) 293
PflMI CCANNNNNTGG 3 cut(s) 329, 488, 529
PfoI TCCNGGA 1 cut(s) 625
PinAI ACCGGT 1 cut(s) 239
PkrI GCNGC 9 cut(s) 106, 130, 133, 233, 236, 337, 898, 946, 951
PleI GAGTC 1 cut(s) 613
PluTI GGCGCC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 613
Ppu21I YACGTR 1 cut(s) 280
PpuMI RGGWCCY 1 cut(s) 353
PshAI GACNNNNGTC 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 353
Psp6I CCWGG 3 cut(s) 287, 514, 625
PspGI CCWGG 3 cut(s) 287, 514, 625
PspN4I GGNNCC 4 cut(s) 78, 153, 204, 521
PspPI GGNCC 7 cut(s) 76, 202, 259, 353, 414, 546, 678
PspPPI RGGWCCY 1 cut(s) 353
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 1 cut(s) 925
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 3 cut(s) 422, 434, 497
RsaNI GTAC 3 cut(s) 421, 433, 496
RseI CAYNNNNRTG 3 cut(s) 71, 831, 919
SaqAI TTAA 1 cut(s) 1081
SatI GCNGC 9 cut(s) 105, 129, 132, 232, 235, 336, 897, 945, 950
Sau3AI GATC 5 cut(s) 123, 282, 426, 809, 925
Sau96I GGNCC 7 cut(s) 76, 202, 259, 353, 414, 546, 678
SchI GAGTC 1 cut(s) 614
ScrFI CCNGG 3 cut(s) 289, 516, 627
SfaNI GCATC 6 cut(s) 309, 620, 622, 748, 832, 847
SfoI GGCGCC 1 cut(s) 521
SgrAI CRCCGGYG 1 cut(s) 239
SinI GGWCC 4 cut(s) 202, 353, 414, 546
SmiMI CAYNNNNRTG 3 cut(s) 71, 831, 919
SmlI CTYRAG 2 cut(s) 9, 762
SmoI CTYRAG 2 cut(s) 9, 762
Sse9I AATT 3 cut(s) 570, 696, 803
SsiI CCGC 7 cut(s) 33, 102, 191, 231, 253, 977, 1007
SspDI GGCGCC 1 cut(s) 519
SspI AATATT 1 cut(s) 736
SspMI CTAG 3 cut(s) 405, 851, 1004
StyD4I CCNGG 3 cut(s) 287, 514, 625
StyI CCWWGG 4 cut(s) 44, 300, 523, 965
TaaI ACNGT 2 cut(s) 54, 148
TaiI ACGT 1 cut(s) 282
TaqI TCGA 3 cut(s) 616, 831, 910
TasI AATT 3 cut(s) 570, 696, 803
TatI WGTACW 1 cut(s) 420
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 1 cut(s) 293
Tru1I TTAA 1 cut(s) 1081
Tru9I TTAA 1 cut(s) 1081
TscAI CASTG 3 cut(s) 93, 392, 592
TseFI GTSAC 3 cut(s) 58, 84, 999
TseI GCWGC 8 cut(s) 104, 128, 131, 234, 335, 896, 944, 949
Tsp45I GTSAC 3 cut(s) 58, 84, 999
TspDTI ATGAA 2 cut(s) 61, 801
TspRI CASTG 3 cut(s) 93, 392, 592
Van91I CCANNNNNTGG 3 cut(s) 329, 488, 529
VpaK11BI GGWCC 4 cut(s) 202, 353, 414, 546
XapI RAATTY 1 cut(s) 803
XspI CTAG 3 cut(s) 405, 851, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.