Rmu_sc0003778.1_g000037

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003778.1
OG0014461
Physical Location & Seq
Reverse (-)
103878 .. 106898
3021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003778.1_g000037.1.cds

Sequence Viewer

Length: 1761 bp
atgggtggtctgagagagtcatggtgcttctgcaaaggagtgagcaagtctgagaagatgaaggcggccattttctccggcaaagctccggcattggcctggatttctggcgccgggaatgggatctccggtaccgggttcttgatccaccgtagcctcctgctgaccacccacgtcaaccttccgtcggtggcggctgccgagagctccgagatccggctgcaaaacggcgtcgctgcaagtctcgttccccacagaaagatcctgctgaccacccacgtcaaccttccgtcggtggcggctgccgagagctccgagatccggctgcaaaacggcgtcgctgcgagtctcgttccccacaggtttttcatcaccagctccgttttagatcttactatagtaggtttggatgcagcagatggagattcaaattctcagggcctcccttactacttgaagacctgctccaaaccaaacctggatctgggaagtggagtttaccttctaggctacaccgagactaaggaattaacagttggcgaaggtaaggtcgttatagccactgacaatctcataaaattgtcaactgatggagtaacatggagccctggttcagctggatttgatgtgcaaggcaaccttgcatttatgatatgcgatcctatgaaactagcaacctcgccaaacaccaaatcctcatctacttcttcatcgtcgtcatcatcatggaaaaaggatcatcctatgcaatttggtatccccattccgatcatatgtgattggttaaatcagcattgggaaggtagccttgatgatcttaacaagcctaagctaccactcattcgattaatgtctactggccaaaaaagtgaccactcctgtgcttccttcacactgcggcgggttttcaaatcaactgaagccgataacgatgggacaccttcttcatcgaatacagtctcaaaacctagagaccagcttggaccaggctgttctgctgcagcaaacacatttgaagaagagaccccaacggatgctcatgctgcccatgtacagggaattcctactcctgaaatatatgaatcaccgaagttaacatctattcctcttcggaaaagagaaggttcccaagttcagcttttggatattaattttcctcctaggatcgctaaaccagcaattacaccgcagcctaccaaaagattacccccgaattctgatgagaattttgtgaagcaatctctattgaatagcccaagaagacaagaaaatcaagtcaagcatagaggaccgggcagccctggtgctgatgctgagattgcttcaacaggttcggtagatggggtccatagtgaggttcagtctagttcatacccagtcgaagcttcagatatgcataatgggtatagtagtgagggagagactatgtactctgcagaaacagctgagagtagaaattatcctagtcctcctgggggaaagtttcagccagtgggaagaagccagagttgtgtgaattataatagatggggagctgttcaaaggaacccagtggctcgaagggcattgctagaacagcaaaggagctttatccatggaaggaagatgtattcacaaggggcaacttctcaaagaagcaatgactactacagcccaactgtgtcctcaatcatgaagaagcgaaactcagagcagcctgtcactactattgtcaaaccccggcaagctgctgttcattcctctccaagatggaacttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

586

Amino Acids

63.23

Weight (kDa)

8.92

Isoelectric Point (pI)

52.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014461)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07210
fragaria_vesca FvH4_2g18560
malus_domestica MD05G1171800.v1.1 MD10G1160100.v1.1
prunus_persica Prupe.8G199200_v2.0.a1
pyrus_communis pycom05g15850 pycom10g13820
rosa_chinensis RchiOBHm_Chr6g0284121
rosa_laevigata RLG00000012768
rosa_multiflora Rmu_sc0003778.1_g000037
rosa_roxburghii Rroxscaffold_7G00184910
rosa_rugosa Rorug06G0161000
rosa_samantha Rh6AG273800 Rh6BG274500 Rh6CG275900 Rh6DG268900
rosa_wichuraiana Rw6G023560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1521
Acc36I ACCTGC 1 cut(s) 470
Acc65I GGTACC 1 cut(s) 131
AccB1I GGYRCC 2 cut(s) 110, 131
AccI GTMKAC 1 cut(s) 854
AciI CCGC 6 cut(s) 65, 194, 299, 898, 901, 1187
AclWI GGATC 9 cut(s) 131, 139, 208, 256, 313, 489, 653, 744, 1172
AcoI YGGCCR 2 cut(s) 66, 859
AcsI RAATTY 4 cut(s) 430, 1059, 1213, 1225
AcuI CTGAAG 2 cut(s) 939, 1371
AcyI GRCGYC 3 cut(s) 111, 231, 336
AfaI GTAC 3 cut(s) 133, 1053, 1430
AgsI TTSAA 7 cut(s) 429, 457, 910, 1016, 1249, 1326, 1541
AjiI CACGTC 2 cut(s) 175, 280
AjnI CCWGG 6 cut(s) 98, 477, 607, 985, 1300, 1471
AjuI GAANNNNNNNTTGG 2 cut(s) 519, 551
AleI CACNNNNGTG 1 cut(s) 879
AloI GAACNNNNNNTCC 2 cut(s) 595, 627
Alw21I GWGCWC 2 cut(s) 209, 314
Alw26I GTCTC 7 cut(s) 248, 353, 512, 964, 966, 1016, 1415
AlwI GGATC 9 cut(s) 131, 139, 208, 256, 313, 489, 653, 744, 1172
AoxI GGCC 4 cut(s) 66, 96, 439, 859
ApoI RAATTY 4 cut(s) 430, 1059, 1213, 1225
ArsI GACNNNNNNTTYG 2 cut(s) 534, 566
AseI ATTAAT 2 cut(s) 848, 1149
Asp718I GGTACC 1 cut(s) 131
AspA2I CCTAGG 1 cut(s) 1160
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 4 cut(s) 439, 983, 1289, 1345
AsuC2I CCSGG 4 cut(s) 115, 136, 1293, 1720
AsuHPI GGTGA 2 cut(s) 364, 1077
AvaII GGWCC 3 cut(s) 983, 1289, 1345
AvrII CCTAGG 1 cut(s) 1160
BalI TGGCCA 1 cut(s) 861
BanI GGYRCC 2 cut(s) 110, 131
BanII GRGCYC 3 cut(s) 209, 314, 608
BbsI GAAGAC 2 cut(s) 464, 1267
Bbv12I GWGCWC 2 cut(s) 209, 314
BccI CCATC 6 cut(s) 413, 584, 926, 1334, 1521, 1743
BceAI ACGGC 2 cut(s) 244, 349
BciT130I CCWGG 6 cut(s) 100, 479, 609, 987, 1302, 1473
BciVI GTATCC 1 cut(s) 767
BcnI CCSGG 4 cut(s) 115, 136, 1293, 1720
BcoDI GTCTC 7 cut(s) 248, 353, 512, 964, 966, 1016, 1415
BfaI CTAG 7 cut(s) 506, 671, 969, 1161, 1365, 1464, 1571
BfmI CTRYAG 4 cut(s) 396, 999, 1434, 1648
BfoI RGCGCY 1 cut(s) 114
BfuAI ACCTGC 1 cut(s) 470
BfuI GTATCC 1 cut(s) 767
BglII AGATCT 1 cut(s) 388
BlnI CCTAGG 1 cut(s) 1160
Bme18I GGWCC 3 cut(s) 983, 1289, 1345
BmgBI CACGTC 2 cut(s) 175, 280
BmgT120I GGNCC 4 cut(s) 439, 983, 1289, 1345
BmiI GGNNCC 6 cut(s) 112, 133, 605, 1126, 1346, 1547
BmrI ACTGGG 2 cut(s) 1370, 1544
BmsI GCATC 3 cut(s) 400, 1024, 1300
BmuI ACTGGG 2 cut(s) 1370, 1544
BpiI GAAGAC 2 cut(s) 464, 1267
Bpu10I CCTNAGC 1 cut(s) 828
BpuMI CCSGG 4 cut(s) 115, 136, 1293, 1720
BsaHI GRCGYC 3 cut(s) 111, 231, 336
BsaI GGTCTC 2 cut(s) 966, 1016
BsaJI CCNNGG 6 cut(s) 607, 1160, 1300, 1472, 1594, 1718
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bse1I ACTGG 4 cut(s) 862, 1376, 1490, 1550
Bse3DI GCAATG 2 cut(s) 1565, 1645
BseBI CCWGG 6 cut(s) 100, 479, 609, 987, 1302, 1473
BseDI CCNNGG 6 cut(s) 607, 1160, 1300, 1472, 1594, 1718
BseGI GGATG 3 cut(s) 415, 739, 1039
BseMI GCAATG 2 cut(s) 1565, 1645
BseMII CTCAG 5 cut(s) 42, 449, 1305, 1437, 1701
BseNI ACTGG 4 cut(s) 862, 1376, 1490, 1550
BshFI GGCC 4 cut(s) 68, 98, 441, 861
BshNI GGYRCC 2 cut(s) 110, 131
BsiHKAI GWGCWC 2 cut(s) 209, 314
BsiSI CCGG 9 cut(s) 78, 89, 114, 129, 135, 217, 322, 1292, 1720
BslFI GGGAC 1 cut(s) 949
BsmAI GTCTC 7 cut(s) 248, 353, 512, 964, 966, 1016, 1415
BsmFI GGGAC 1 cut(s) 949
BsnI GGCC 4 cut(s) 68, 98, 441, 861
Bso31I GGTCTC 2 cut(s) 966, 1016
Bsp1286I GDGCHC 3 cut(s) 209, 314, 608
Bsp1407I TGTACA 1 cut(s) 1051
Bsp19I CCATGG 1 cut(s) 1594
BspACI CCGC 6 cut(s) 65, 194, 299, 898, 901, 1187
BspANI GGCC 4 cut(s) 68, 98, 441, 861
BspCNI CTCAG 5 cut(s) 43, 448, 1306, 1438, 1700
BspHI TCATGA 1 cut(s) 1671
BspLI GGNNCC 6 cut(s) 112, 133, 605, 1126, 1346, 1547
BspMAI CTGCAG 2 cut(s) 1003, 1438
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 9 cut(s) 131, 139, 208, 256, 313, 489, 653, 744, 1172
BspT107I GGYRCC 2 cut(s) 110, 131
BspTNI GGTCTC 2 cut(s) 966, 1016
BsrDI GCAATG 2 cut(s) 1565, 1645
BsrGI TGTACA 1 cut(s) 1051
BsrI ACTGG 4 cut(s) 862, 1376, 1490, 1550
BssECI CCNNGG 6 cut(s) 607, 1160, 1300, 1472, 1594, 1718
BssNI GRCGYC 3 cut(s) 111, 231, 336
BssT1I CCWWGG 2 cut(s) 1160, 1594
Bst2UI CCWGG 6 cut(s) 100, 479, 609, 987, 1302, 1473
Bst4CI ACNGT 4 cut(s) 152, 535, 958, 1660
Bst6I CTCTTC 2 cut(s) 1014, 1113
BstACI GRCGYC 3 cut(s) 111, 231, 336
BstAUI TGTACA 1 cut(s) 1051
BstC8I GCNNGC 1 cut(s) 1725
BstDEI CTNAG 8 cut(s) 11, 51, 435, 522, 828, 1314, 1446, 1687
BstDSI CCRYGG 1 cut(s) 1594
BstF5I GGATG 3 cut(s) 415, 739, 1039
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstMAI GTCTC 7 cut(s) 248, 353, 512, 964, 966, 1016, 1415
BstMWI GCNNNNNNNGC 6 cut(s) 1043, 1175, 1319, 1442, 1562, 1576
BstNI CCWGG 6 cut(s) 100, 479, 609, 987, 1302, 1473
BstSFI CTRYAG 4 cut(s) 396, 999, 1434, 1648
BstV2I GAAGAC 2 cut(s) 464, 1267
BstX2I RGATCY 6 cut(s) 123, 213, 261, 318, 388, 481
BstYI RGATCY 6 cut(s) 123, 213, 261, 318, 388, 481
BsuI GTATCC 1 cut(s) 767
BsuRI GGCC 4 cut(s) 68, 98, 441, 861
BtgI CCRYGG 1 cut(s) 1594
BtrI CACGTC 2 cut(s) 175, 280
BtsCI GGATG 3 cut(s) 415, 739, 1039
BtsI GCAGTG 1 cut(s) 893
BtsIMutI CAGTG 4 cut(s) 561, 893, 1497, 1557
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 1725
CciI TCATGA 1 cut(s) 1671
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 4 cut(s) 439, 983, 1289, 1345
CseI GACGC 2 cut(s) 220, 325
Csp6I GTAC 3 cut(s) 132, 1052, 1429
CviAII CATG 7 cut(s) 21, 600, 726, 1040, 1049, 1595, 1672
CviQI GTAC 3 cut(s) 132, 1052, 1429
DdeI CTNAG 8 cut(s) 11, 51, 435, 522, 828, 1314, 1446, 1687
DinI GGCGCC 1 cut(s) 112
EaeI YGGCCR 2 cut(s) 66, 859
Eam1104I CTCTTC 2 cut(s) 1014, 1113
EarI CTCTTC 2 cut(s) 1014, 1113
Ecl136II GAGCTC 2 cut(s) 207, 312
Eco130I CCWWGG 2 cut(s) 1160, 1594
Eco24I GRGCYC 3 cut(s) 209, 314, 608
Eco31I GGTCTC 2 cut(s) 966, 1016
Eco47I GGWCC 3 cut(s) 983, 1289, 1345
Eco53kI GAGCTC 2 cut(s) 207, 312
Eco57I CTGAAG 2 cut(s) 939, 1371
EcoICRI GAGCTC 2 cut(s) 207, 312
EcoO109I RGGNCCY 1 cut(s) 439
EcoRI GAATTC 2 cut(s) 1059, 1213
EcoRII CCWGG 6 cut(s) 98, 477, 607, 985, 1300, 1471
EcoT14I CCWWGG 2 cut(s) 1160, 1594
EcoT22I ATGCAT 1 cut(s) 1398
EcoT38I GRGCYC 3 cut(s) 209, 314, 608
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 2 cut(s) 1160, 1594
FaeI CATG 7 cut(s) 24, 603, 729, 1043, 1052, 1598, 1675
FalI AAGNNNNNCTT 6 cut(s) 624, 656, 792, 824, 1122, 1154
FaqI GGGAC 1 cut(s) 949
FatI CATG 7 cut(s) 20, 599, 725, 1039, 1048, 1594, 1671
FauI CCCGC 1 cut(s) 894
FauNDI CATATG 1 cut(s) 773
FblI GTMKAC 1 cut(s) 854
FokI GGATG 3 cut(s) 422, 726, 1046
FriOI GRGCYC 3 cut(s) 209, 314, 608
FspBI CTAG 7 cut(s) 506, 671, 969, 1161, 1365, 1464, 1571
GlaI GCGC 1 cut(s) 112
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 4 cut(s) 68, 98, 441, 861
HapII CCGG 9 cut(s) 78, 89, 114, 129, 135, 217, 322, 1292, 1720
HgaI GACGC 2 cut(s) 220, 325
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 3 cut(s) 111, 231, 336
Hin1II CATG 7 cut(s) 24, 603, 729, 1043, 1052, 1598, 1675
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 4 cut(s) 178, 283, 585, 1095
HindII GTYRAC 4 cut(s) 178, 283, 585, 1095
HindIII AAGCTT 1 cut(s) 1383
HinfI GANTC 4 cut(s) 17, 346, 425, 1082
HpaI GTTAAC 1 cut(s) 1095
HpaII CCGG 9 cut(s) 78, 89, 114, 129, 135, 217, 322, 1292, 1720
HphI GGTGA 2 cut(s) 364, 1077
Hpy166II GTNNAC 6 cut(s) 178, 283, 499, 585, 855, 1095
Hpy188I TCNGA 9 cut(s) 12, 52, 211, 316, 768, 1113, 1219, 1390, 1690
Hpy188III TCNNGA 3 cut(s) 142, 1070, 1672
Hpy8I GTNNAC 6 cut(s) 178, 283, 499, 585, 855, 1095
Hpy99I CGWCG 5 cut(s) 190, 236, 295, 341, 718
HpyCH4III ACNGT 4 cut(s) 152, 535, 958, 1660
HpyCH4IV ACGT 2 cut(s) 174, 279
HpyF10VI GCNNNNNNNGC 6 cut(s) 1043, 1175, 1319, 1442, 1562, 1576
HpyF3I CTNAG 8 cut(s) 11, 51, 435, 522, 828, 1314, 1446, 1687
HpySE526I ACGT 2 cut(s) 174, 279
Hsp92I GRCGYC 3 cut(s) 111, 231, 336
Hsp92II CATG 7 cut(s) 24, 603, 729, 1043, 1052, 1598, 1675
HspAI GCGC 1 cut(s) 111
KasI GGCGCC 1 cut(s) 110
KpnI GGTACC 1 cut(s) 135
KspAI GTTAAC 1 cut(s) 1095
LmnI GCTCC 8 cut(s) 91, 212, 317, 383, 470, 603, 1532, 1584
LweI GCATC 3 cut(s) 400, 1024, 1300
MaeI CTAG 7 cut(s) 506, 671, 969, 1161, 1365, 1464, 1571
MaeII ACGT 2 cut(s) 174, 279
MaeIII GTNAC 3 cut(s) 595, 869, 1699
MflI RGATCY 6 cut(s) 123, 213, 261, 318, 388, 481
MhlI GDGCHC 3 cut(s) 209, 314, 608
MlsI TGGCCA 1 cut(s) 861
MluNI TGGCCA 1 cut(s) 861
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 2 cut(s) 26, 355
Mox20I TGGCCA 1 cut(s) 861
Mph1103I ATGCAT 1 cut(s) 1398
MscI TGGCCA 1 cut(s) 861
MseI TTAA 6 cut(s) 530, 785, 819, 848, 1094, 1149
MslI CAYNNNNRTG 2 cut(s) 724, 879
Msp20I TGGCCA 1 cut(s) 861
MspA1I CMGCKG 2 cut(s) 617, 1445
MspI CCGG 9 cut(s) 78, 89, 114, 129, 135, 217, 322, 1292, 1720
MvaI CCWGG 6 cut(s) 100, 479, 609, 987, 1302, 1473
MwoI GCNNNNNNNGC 6 cut(s) 1043, 1175, 1319, 1442, 1562, 1576
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 4 cut(s) 115, 136, 1293, 1720
NcoI CCATGG 1 cut(s) 1594
NdeI CATATG 1 cut(s) 773
NlaIII CATG 7 cut(s) 24, 603, 729, 1043, 1052, 1598, 1675
NlaIV GGNNCC 6 cut(s) 112, 133, 605, 1126, 1346, 1547
NmeAIII GCCGAG 2 cut(s) 226, 331
NmuCI GTSAC 2 cut(s) 869, 1699
NsiI ATGCAT 1 cut(s) 1398
OliI CACNNNNGTG 1 cut(s) 879
PagI TCATGA 1 cut(s) 1671
PfeI GAWTC 2 cut(s) 425, 1082
PleI GAGTC 2 cut(s) 25, 354
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 2 cut(s) 25, 354
PshBI ATTAAT 2 cut(s) 848, 1149
PsiI TTATAA 1 cut(s) 1521
Psp124BI GAGCTC 2 cut(s) 209, 314
Psp6I CCWGG 6 cut(s) 98, 477, 607, 985, 1300, 1471
PspGI CCWGG 6 cut(s) 98, 477, 607, 985, 1300, 1471
PspN4I GGNNCC 6 cut(s) 112, 133, 605, 1126, 1346, 1547
PspPI GGNCC 4 cut(s) 439, 983, 1289, 1345
PstI CTGCAG 2 cut(s) 1003, 1438
PsuI RGATCY 6 cut(s) 123, 213, 261, 318, 388, 481
PvuII CAGCTG 2 cut(s) 617, 1445
RsaI GTAC 3 cut(s) 133, 1053, 1430
RsaNI GTAC 3 cut(s) 132, 1052, 1429
RseI CAYNNNNRTG 2 cut(s) 724, 879
SacI GAGCTC 2 cut(s) 209, 314
SaqAI TTAA 6 cut(s) 530, 785, 819, 848, 1094, 1149
Sau96I GGNCC 4 cut(s) 439, 983, 1289, 1345
SchI GAGTC 2 cut(s) 26, 355
SduI GDGCHC 3 cut(s) 209, 314, 608
SfaNI GCATC 3 cut(s) 400, 1024, 1300
SfcI CTRYAG 4 cut(s) 396, 999, 1434, 1648
SfoI GGCGCC 1 cut(s) 112
SinI GGWCC 3 cut(s) 983, 1289, 1345
SmiMI CAYNNNNRTG 2 cut(s) 724, 879
SsiI CCGC 6 cut(s) 65, 194, 299, 898, 901, 1187
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 7 cut(s) 506, 671, 969, 1161, 1365, 1464, 1571
SstI GAGCTC 2 cut(s) 209, 314
StyI CCWWGG 2 cut(s) 1160, 1594
TaaI ACNGT 4 cut(s) 152, 535, 958, 1660
TaiI ACGT 2 cut(s) 177, 282
TaqI TCGA 4 cut(s) 844, 950, 1380, 1558
TatI WGTACW 2 cut(s) 1051, 1428
TauI GCSGC 4 cut(s) 68, 197, 302, 901
TfiI GAWTC 2 cut(s) 425, 1082
Tru1I TTAA 6 cut(s) 530, 785, 819, 848, 1094, 1149
Tru9I TTAA 6 cut(s) 530, 785, 819, 848, 1094, 1149
TscAI CASTG 4 cut(s) 568, 900, 1497, 1557
TseFI GTSAC 2 cut(s) 869, 1699
Tsp45I GTSAC 2 cut(s) 869, 1699
TspDTI ATGAA 9 cut(s) 74, 358, 680, 699, 936, 1095, 1359, 1688, 1724
TspGWI ACGGA 4 cut(s) 174, 279, 370, 1046
TspRI CASTG 4 cut(s) 568, 900, 1497, 1557
VpaK11BI GGWCC 3 cut(s) 983, 1289, 1345
VspI ATTAAT 2 cut(s) 848, 1149
XapI RAATTY 4 cut(s) 430, 1059, 1213, 1225
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XmaJI CCTAGG 1 cut(s) 1160
XmiI GTMKAC 1 cut(s) 854
XspI CTAG 7 cut(s) 506, 671, 969, 1161, 1365, 1464, 1571
Zsp2I ATGCAT 1 cut(s) 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.