Rh6BG274500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0014461
Physical Location & Seq
Forward (+)
49520203 .. 49523775
3573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG274500.1

Sequence Viewer

Length: 1413 bp
ATGGAGTTTCAGCTGTTTTTCATCACCAGCTCCGTTTTAGATCTTACTATAGTAGGTTTGGATGCAGCAGATGGAGATTCAAATTCTCAGGGCCTCCCTTACTACTTGAAGACCTGCTCCAAACCAAACCTGGATCTGGGAAGTGGTGTTTACCTTCTAGGCTACACCGAGACTAAGGAATTAACAGTTGGCGAAGGTAAGGTCGTTATAGCCACTGACAATCTCATAAAATTGTCAACTGATGGAGTAACATGGAGCCCTGGTTCAGCTGGATTTGATGTGCAAGGCAACCTTGCATTTATGATATGCGATCCTATGAAACTAGCAACCTCGCCAAACACCAAATCCTCATCTACTTCTTCATCATCGTCATCATCATGGAAAAAGGATCATCCTATGCAATTTGGTATCCCCATTCCGATCATATGTGATTGGTTAAATCAGCATTGGGAAGGTAGCCTTGATGATCTTAACAAGCCTAAGCTACCACTCATTCGATTAATGTCTACTGGCCAAAAAAGTGACCACTCCTGTGCTTCCTTCACACTGCGGCGGGTTTTCAAATCAACTGAAGCCGATAACGATGGGACACCTTCTTCATCGAATACAGTCTCAAAACCTAGAGACCAGCTTGGACCAGGCTGTTCTGCTGCAGCAAACACATTTGAAGAAGAGACCCCAACGGATGCTCATGCTGCCCATATACAGGGAATTCCTACTCCTGAAATATATGAATCACCGAAGTTAACATCTATTCCTCTTCGGAAAAGAGAAGGTTCCCAAGTTCAGCTTTTGGATATTAATTTTCCTCCTAGGATCGCTAAACCAGCAATTACACCGCAGCCTACCAAAAGATTACCCCCGAATTCTGATGAGAATTTTGTGAAGCAATCTCTATTGAATAGTCCAAGAAGACAAGAAAATCAAGTCAAGGATAGAGGACCGGACAGCCCTGGTGCTGATGCTGAGATTGCTTCAACAGGTTCGGTAGATGGGGTCCATAGTGAGGTTCAGTCTAGTTCATACCCAGTCGAAGCTTCAGATATGCATAATGGGTATAGTAGTGAGGGAGAGACTATGTACTCTGCAGAAACAGCTGAGAGTAGAAATTATCCTAGTCCTCCTGGGGGAAAGTTTCAGCCAGTGGGAAGAAGCCAGAGTTGTGTGAATTATAATAGATGGGGAGCTGTTCAAAGGAACCCAGTGGCTCGAAGGGCATTGCTAGAACAGCAAAGGAGCTTTATCCATGGAAGGAAGATGTATTCACAAGGGGCAACTTCTCAAAGAAGCAATGACTACTACAGCCCAACTGTGTCCTCAATCATGAAGAAGCGAAACTCAGAGCAGCCTGTCACTACTATTGTCAAACCCCGGCAAGCTGCTGTTCATTCCTCTCCAAGATGGAACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

470

Amino Acids

51.32

Weight (kDa)

6.77

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014461)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07210
fragaria_vesca FvH4_2g18560
malus_domestica MD05G1171800.v1.1 MD10G1160100.v1.1
prunus_persica Prupe.8G199200_v2.0.a1
pyrus_communis pycom05g15850 pycom10g13820
rosa_chinensis RchiOBHm_Chr6g0284121
rosa_laevigata RLG00000012768
rosa_multiflora Rmu_sc0003778.1_g000037
rosa_roxburghii Rroxscaffold_7G00184910
rosa_rugosa Rorug06G0161000
rosa_samantha Rh6AG273800 Rh6BG274500 Rh6CG275900 Rh6DG268900
rosa_wichuraiana Rw6G023560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1173
Acc36I ACCTGC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 506
AciI CCGC 3 cut(s) 550, 553, 839
AclWI GGATC 4 cut(s) 141, 305, 396, 824
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 4 cut(s) 82, 711, 865, 877
AcuI CTGAAG 2 cut(s) 591, 1023
AfaI GTAC 1 cut(s) 1082
AfiI CCNNNNNNNGG 4 cut(s) 136, 706, 1006, 1127
AgsI TTSAA 7 cut(s) 81, 109, 562, 668, 901, 978, 1193
AjnI CCWGG 5 cut(s) 129, 259, 637, 952, 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AleI CACNNNNGTG 1 cut(s) 531
AloI GAACNNNNNNTCC 2 cut(s) 247, 279
Alw26I GTCTC 5 cut(s) 164, 616, 618, 668, 1067
AlwI GGATC 4 cut(s) 141, 305, 396, 824
AoxI GGCC 2 cut(s) 91, 511
ApeKI GCWGC 7 cut(s) 65, 650, 653, 695, 841, 1345, 1379
ApoI RAATTY 4 cut(s) 82, 711, 865, 877
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AseI ATTAAT 2 cut(s) 500, 801
AspA2I CCTAGG 1 cut(s) 812
AspS9I GGNCC 4 cut(s) 91, 635, 941, 997
AsuC2I CCSGG 1 cut(s) 1372
AsuHPI GGTGA 2 cut(s) 16, 729
AvaII GGWCC 3 cut(s) 635, 941, 997
AvrII CCTAGG 1 cut(s) 812
BalI TGGCCA 1 cut(s) 513
BanII GRGCYC 1 cut(s) 260
BbsI GAAGAC 2 cut(s) 116, 919
BbvI GCAGC 7 cut(s) 77, 637, 665, 682, 853, 1357, 1366
BccI CCATC 6 cut(s) 65, 236, 578, 986, 1173, 1395
BciT130I CCWGG 5 cut(s) 131, 261, 639, 954, 1125
BciVI GTATCC 1 cut(s) 419
BcnI CCSGG 1 cut(s) 1372
BcoDI GTCTC 5 cut(s) 164, 616, 618, 668, 1067
BfaI CTAG 7 cut(s) 158, 323, 621, 813, 1017, 1116, 1223
BfmI CTRYAG 4 cut(s) 48, 651, 1086, 1300
BfuAI ACCTGC 1 cut(s) 122
BfuI GTATCC 1 cut(s) 419
BglII AGATCT 1 cut(s) 40
BisI GCNGC 8 cut(s) 66, 551, 651, 654, 696, 842, 1346, 1380
BlnI CCTAGG 1 cut(s) 812
BlsI GCNGC 8 cut(s) 67, 552, 652, 655, 697, 843, 1347, 1381
Bme1390I CCNGG 6 cut(s) 131, 261, 639, 954, 1125, 1372
Bme18I GGWCC 3 cut(s) 635, 941, 997
BmgT120I GGNCC 4 cut(s) 91, 635, 941, 997
BmiI GGNNCC 4 cut(s) 257, 778, 998, 1199
BmrFI CCNGG 6 cut(s) 131, 261, 639, 954, 1125, 1372
BmrI ACTGGG 2 cut(s) 1022, 1196
BmsI GCATC 3 cut(s) 52, 676, 952
BmuI ACTGGG 2 cut(s) 1022, 1196
BpiI GAAGAC 2 cut(s) 116, 919
Bpu10I CCTNAGC 1 cut(s) 480
BpuMI CCSGG 1 cut(s) 1372
BsaI GGTCTC 2 cut(s) 618, 668
BsaJI CCNNGG 6 cut(s) 259, 812, 952, 1124, 1246, 1370
BsaWI WCCGGW 1 cut(s) 943
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 4 cut(s) 136, 706, 1006, 1127
Bse1I ACTGG 4 cut(s) 514, 1028, 1142, 1202
Bse3DI GCAATG 2 cut(s) 1217, 1297
BseBI CCWGG 5 cut(s) 131, 261, 639, 954, 1125
BseDI CCNNGG 6 cut(s) 259, 812, 952, 1124, 1246, 1370
BseGI GGATG 3 cut(s) 67, 391, 691
BseLI CCNNNNNNNGG 4 cut(s) 136, 706, 1006, 1127
BseMI GCAATG 2 cut(s) 1217, 1297
BseMII CTCAG 4 cut(s) 101, 957, 1089, 1353
BseNI ACTGG 4 cut(s) 514, 1028, 1142, 1202
BseXI GCAGC 7 cut(s) 77, 637, 665, 682, 853, 1357, 1366
BshFI GGCC 2 cut(s) 93, 513
BsiSI CCGG 2 cut(s) 944, 1372
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 4 cut(s) 136, 706, 1006, 1127
BsmAI GTCTC 5 cut(s) 164, 616, 618, 668, 1067
BsmFI GGGAC 1 cut(s) 601
BsnI GGCC 2 cut(s) 93, 513
Bso31I GGTCTC 2 cut(s) 618, 668
Bsp1286I GDGCHC 1 cut(s) 260
Bsp143I GATC 7 cut(s) 40, 133, 310, 388, 420, 466, 816
Bsp19I CCATGG 1 cut(s) 1246
BspACI CCGC 3 cut(s) 550, 553, 839
BspANI GGCC 2 cut(s) 93, 513
BspCNI CTCAG 4 cut(s) 100, 958, 1090, 1352
BspHI TCATGA 1 cut(s) 1323
BspLI GGNNCC 4 cut(s) 257, 778, 998, 1199
BspMAI CTGCAG 2 cut(s) 655, 1090
BspMI ACCTGC 1 cut(s) 122
BspPI GGATC 4 cut(s) 141, 305, 396, 824
BspTNI GGTCTC 2 cut(s) 618, 668
BsrDI GCAATG 2 cut(s) 1217, 1297
BsrI ACTGG 4 cut(s) 514, 1028, 1142, 1202
BssECI CCNNGG 6 cut(s) 259, 812, 952, 1124, 1246, 1370
BssMI GATC 7 cut(s) 40, 133, 310, 388, 420, 466, 816
BssT1I CCWWGG 2 cut(s) 812, 1246
Bst2UI CCWGG 5 cut(s) 131, 261, 639, 954, 1125
Bst4CI ACNGT 3 cut(s) 187, 610, 1312
Bst6I CTCTTC 2 cut(s) 666, 765
BstC8I GCNNGC 1 cut(s) 1377
BstDEI CTNAG 6 cut(s) 87, 174, 480, 966, 1098, 1339
BstDSI CCRYGG 1 cut(s) 1246
BstF5I GGATG 3 cut(s) 67, 391, 691
BstKTI GATC 7 cut(s) 43, 136, 313, 391, 423, 469, 819
BstMAI GTCTC 5 cut(s) 164, 616, 618, 668, 1067
BstMBI GATC 7 cut(s) 40, 133, 310, 388, 420, 466, 816
BstMWI GCNNNNNNNGC 6 cut(s) 695, 827, 971, 1094, 1214, 1228
BstNI CCWGG 5 cut(s) 131, 261, 639, 954, 1125
BstSCI CCNGG 6 cut(s) 129, 259, 637, 952, 1123, 1370
BstSFI CTRYAG 4 cut(s) 48, 651, 1086, 1300
BstV1I GCAGC 7 cut(s) 77, 637, 665, 682, 853, 1357, 1366
BstV2I GAAGAC 2 cut(s) 116, 919
BstX2I RGATCY 2 cut(s) 40, 133
BstYI RGATCY 2 cut(s) 40, 133
BsuI GTATCC 1 cut(s) 419
BsuRI GGCC 2 cut(s) 93, 513
BtgI CCRYGG 1 cut(s) 1246
BtsCI GGATG 3 cut(s) 67, 391, 691
BtsI GCAGTG 1 cut(s) 545
BtsIMutI CAGTG 4 cut(s) 213, 545, 1149, 1209
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 1 cut(s) 1377
CciI TCATGA 1 cut(s) 1323
Cfr13I GGNCC 4 cut(s) 91, 635, 941, 997
Csp6I GTAC 1 cut(s) 1081
CviAII CATG 5 cut(s) 252, 378, 692, 1247, 1324
CviQI GTAC 1 cut(s) 1081
DdeI CTNAG 6 cut(s) 87, 174, 480, 966, 1098, 1339
DpnI GATC 7 cut(s) 42, 135, 312, 390, 422, 468, 818
DpnII GATC 7 cut(s) 40, 133, 310, 388, 420, 466, 816
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 2 cut(s) 666, 765
EarI CTCTTC 2 cut(s) 666, 765
Eco130I CCWWGG 2 cut(s) 812, 1246
Eco24I GRGCYC 1 cut(s) 260
Eco31I GGTCTC 2 cut(s) 618, 668
Eco47I GGWCC 3 cut(s) 635, 941, 997
Eco57I CTGAAG 2 cut(s) 591, 1023
EcoO109I RGGNCCY 1 cut(s) 91
EcoRI GAATTC 2 cut(s) 711, 865
EcoRII CCWGG 5 cut(s) 129, 259, 637, 952, 1123
EcoT14I CCWWGG 2 cut(s) 812, 1246
EcoT22I ATGCAT 1 cut(s) 1050
EcoT38I GRGCYC 1 cut(s) 260
ErhI CCWWGG 2 cut(s) 812, 1246
FaeI CATG 5 cut(s) 255, 381, 695, 1250, 1327
FalI AAGNNNNNCTT 6 cut(s) 276, 308, 444, 476, 774, 806
FaqI GGGAC 1 cut(s) 601
FatI CATG 5 cut(s) 251, 377, 691, 1246, 1323
FauI CCCGC 1 cut(s) 546
FauNDI CATATG 1 cut(s) 425
FblI GTMKAC 1 cut(s) 506
Fnu4HI GCNGC 8 cut(s) 66, 551, 651, 654, 696, 842, 1346, 1380
FokI GGATG 3 cut(s) 74, 378, 698
FriOI GRGCYC 1 cut(s) 260
Fsp4HI GCNGC 8 cut(s) 66, 551, 651, 654, 696, 842, 1346, 1380
FspBI CTAG 7 cut(s) 158, 323, 621, 813, 1017, 1116, 1223
GluI GCNGC 8 cut(s) 66, 551, 651, 654, 696, 842, 1346, 1380
HaeIII GGCC 2 cut(s) 93, 513
HapII CCGG 2 cut(s) 944, 1372
Hin1II CATG 5 cut(s) 255, 381, 695, 1250, 1327
HincII GTYRAC 2 cut(s) 237, 747
HindII GTYRAC 2 cut(s) 237, 747
HindIII AAGCTT 1 cut(s) 1035
HinfI GANTC 2 cut(s) 77, 734
HpaI GTTAAC 1 cut(s) 747
HpaII CCGG 2 cut(s) 944, 1372
HphI GGTGA 2 cut(s) 16, 729
Hpy166II GTNNAC 4 cut(s) 151, 237, 507, 747
Hpy188I TCNGA 5 cut(s) 420, 765, 871, 1042, 1342
Hpy188III TCNNGA 2 cut(s) 722, 1324
Hpy8I GTNNAC 4 cut(s) 151, 237, 507, 747
HpyAV CCTTC 8 cut(s) 164, 188, 446, 550, 603, 767, 1206, 1245
HpyCH4III ACNGT 3 cut(s) 187, 610, 1312
HpyCH4V TGCA 7 cut(s) 65, 283, 296, 400, 653, 1048, 1088
HpyF10VI GCNNNNNNNGC 6 cut(s) 695, 827, 971, 1094, 1214, 1228
HpyF3I CTNAG 6 cut(s) 87, 174, 480, 966, 1098, 1339
Hsp92II CATG 5 cut(s) 255, 381, 695, 1250, 1327
KspAI GTTAAC 1 cut(s) 747
Kzo9I GATC 7 cut(s) 40, 133, 310, 388, 420, 466, 816
LmnI GCTCC 5 cut(s) 35, 122, 255, 1184, 1236
Lsp1109I GCAGC 7 cut(s) 77, 637, 665, 682, 853, 1357, 1366
LweI GCATC 3 cut(s) 52, 676, 952
MaeI CTAG 7 cut(s) 158, 323, 621, 813, 1017, 1116, 1223
MaeIII GTNAC 3 cut(s) 247, 521, 1351
MalI GATC 7 cut(s) 42, 135, 312, 390, 422, 468, 818
MboI GATC 7 cut(s) 40, 133, 310, 388, 420, 466, 816
MflI RGATCY 2 cut(s) 40, 133
MhlI GDGCHC 1 cut(s) 260
MlsI TGGCCA 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 513
Mox20I TGGCCA 1 cut(s) 513
Mph1103I ATGCAT 1 cut(s) 1050
MscI TGGCCA 1 cut(s) 513
MseI TTAA 6 cut(s) 182, 437, 471, 500, 746, 801
MslI CAYNNNNRTG 2 cut(s) 376, 531
Msp20I TGGCCA 1 cut(s) 513
MspA1I CMGCKG 3 cut(s) 13, 269, 1097
MspI CCGG 2 cut(s) 944, 1372
MspR9I CCNGG 6 cut(s) 131, 261, 639, 954, 1125, 1372
MvaI CCWGG 5 cut(s) 131, 261, 639, 954, 1125
MwoI GCNNNNNNNGC 6 cut(s) 695, 827, 971, 1094, 1214, 1228
NciI CCSGG 1 cut(s) 1372
NcoI CCATGG 1 cut(s) 1246
NdeI CATATG 1 cut(s) 425
NdeII GATC 7 cut(s) 40, 133, 310, 388, 420, 466, 816
NlaIII CATG 5 cut(s) 255, 381, 695, 1250, 1327
NlaIV GGNNCC 4 cut(s) 257, 778, 998, 1199
NmuCI GTSAC 2 cut(s) 521, 1351
NsiI ATGCAT 1 cut(s) 1050
OliI CACNNNNGTG 1 cut(s) 531
PagI TCATGA 1 cut(s) 1323
PfeI GAWTC 2 cut(s) 77, 734
PkrI GCNGC 8 cut(s) 67, 552, 652, 655, 697, 843, 1347, 1381
PshBI ATTAAT 2 cut(s) 500, 801
PsiI TTATAA 1 cut(s) 1173
Psp6I CCWGG 5 cut(s) 129, 259, 637, 952, 1123
PspGI CCWGG 5 cut(s) 129, 259, 637, 952, 1123
PspN4I GGNNCC 4 cut(s) 257, 778, 998, 1199
PspPI GGNCC 4 cut(s) 91, 635, 941, 997
PstI CTGCAG 2 cut(s) 655, 1090
PsuI RGATCY 2 cut(s) 40, 133
PvuII CAGCTG 3 cut(s) 13, 269, 1097
RsaI GTAC 1 cut(s) 1082
RsaNI GTAC 1 cut(s) 1081
RseI CAYNNNNRTG 2 cut(s) 376, 531
SaqAI TTAA 6 cut(s) 182, 437, 471, 500, 746, 801
SatI GCNGC 8 cut(s) 66, 551, 651, 654, 696, 842, 1346, 1380
Sau3AI GATC 7 cut(s) 40, 133, 310, 388, 420, 466, 816
Sau96I GGNCC 4 cut(s) 91, 635, 941, 997
ScrFI CCNGG 6 cut(s) 131, 261, 639, 954, 1125, 1372
SduI GDGCHC 1 cut(s) 260
SfaNI GCATC 3 cut(s) 52, 676, 952
SfcI CTRYAG 4 cut(s) 48, 651, 1086, 1300
SinI GGWCC 3 cut(s) 635, 941, 997
SmiMI CAYNNNNRTG 2 cut(s) 376, 531
SsiI CCGC 3 cut(s) 550, 553, 839
SspMI CTAG 7 cut(s) 158, 323, 621, 813, 1017, 1116, 1223
StyD4I CCNGG 6 cut(s) 129, 259, 637, 952, 1123, 1370
StyI CCWWGG 2 cut(s) 812, 1246
TaaI ACNGT 3 cut(s) 187, 610, 1312
TaqI TCGA 4 cut(s) 496, 602, 1032, 1210
TatI WGTACW 1 cut(s) 1080
TauI GCSGC 1 cut(s) 553
TfiI GAWTC 2 cut(s) 77, 734
Tru1I TTAA 6 cut(s) 182, 437, 471, 500, 746, 801
Tru9I TTAA 6 cut(s) 182, 437, 471, 500, 746, 801
TscAI CASTG 4 cut(s) 220, 552, 1149, 1209
TseFI GTSAC 2 cut(s) 521, 1351
TseI GCWGC 7 cut(s) 65, 650, 653, 695, 841, 1345, 1379
Tsp45I GTSAC 2 cut(s) 521, 1351
TspDTI ATGAA 8 cut(s) 10, 332, 351, 588, 747, 1011, 1340, 1376
TspGWI ACGGA 2 cut(s) 22, 698
TspRI CASTG 4 cut(s) 220, 552, 1149, 1209
VpaK11BI GGWCC 3 cut(s) 635, 941, 997
VspI ATTAAT 2 cut(s) 500, 801
XapI RAATTY 4 cut(s) 82, 711, 865, 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmaJI CCTAGG 1 cut(s) 812
XmiI GTMKAC 1 cut(s) 506
XspI CTAG 7 cut(s) 158, 323, 621, 813, 1017, 1116, 1223
Zsp2I ATGCAT 1 cut(s) 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.