Rmu_sc0003863.1_g000012

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003863.1
OG0019492
Physical Location & Seq
Forward (+)
71267 .. 71785
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003863.1_g000012.1.cds

Sequence Viewer

Length: 519 bp
atggcagcaaattgtaccatgattggagaacagggttatgatcgagctgaagaagtaaagcaatttgacggctccaagctcggagtcaaaagccttgtcgactcgggtctctcctccatcccccgaatctttatccacccacccataatatcacatcaattccagcccacgacccaacctgacgccgaatcaatccccaccatcaacctctccgacattcactctgatcgtcgcgccgccatcgtcgacccatttaattttaaccaccacacagtgttagatttgaacaattcaagatccaagcgggttcagacaacaccagacataataaacgatctccccgccgcctccttgctcttcacgacgtcggtgtggagaaactcaaccagatggccggagtgggaggcccattgggcggaggagatgttgaggtcgagggagagatctcctctggtcgacggtgtcaggaagttggtcttggacttgttgccgcggagagacctggaggggccgtcgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

172

Amino Acids

19.45

Weight (kDa)

5.84

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019492)

Species Orthologous Gene IDs
rosa_laevigata RLG00000026769 RLG00000031276
rosa_multiflora Rmu_sc0000366.1_g000015 Rmu_sc0003863.1_g000012
rosa_samantha Rh4DG099900 Rh6BG445900 Rh6DG455900 Rh7AG260300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 368
AccI GTMKAC 3 cut(s) 99, 246, 456
AccII CGCG 2 cut(s) 234, 493
AciI CCGC 7 cut(s) 237, 304, 342, 345, 416, 491, 493
AclWI GGATC 1 cut(s) 291
AcoI YGGCCR 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 69
AcyI GRCGYC 2 cut(s) 183, 365
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 1 cut(s) 415
AgsI TTSAA 2 cut(s) 286, 294
AjnI CCWGG 1 cut(s) 501
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 2 cut(s) 47, 79
AluI AGCT 2 cut(s) 47, 79
Alw26I GTCTC 2 cut(s) 113, 492
AlwI GGATC 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 103
AoxI GGCC 3 cut(s) 392, 405, 509
ApeKI GCWGC 1 cut(s) 5
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 2 cut(s) 406, 509
AvaI CYCGRG 1 cut(s) 103
BbvI GCAGC 1 cut(s) 17
BccI CCATC 4 cut(s) 125, 209, 248, 384
BceAI ACGGC 2 cut(s) 85, 496
BciT130I CCWGG 1 cut(s) 503
BcoDI GTCTC 2 cut(s) 113, 492
BglI GCCNNNNNGGC 1 cut(s) 413
BglII AGATCT 1 cut(s) 443
BisI GCNGC 4 cut(s) 6, 237, 345, 491
BlsI GCNGC 4 cut(s) 7, 238, 346, 492
Bme1390I CCNGG 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 406, 509
BmiI GGNNCC 2 cut(s) 73, 510
BmrFI CCNGG 1 cut(s) 503
BoxI GACNNNNGTC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 433, 465
BsaHI GRCGYC 2 cut(s) 183, 365
BsaI GGTCTC 2 cut(s) 113, 492
BsaJI CCNNGG 1 cut(s) 491
BsaXI ACNNNNNCTCC 3 cut(s) 410, 440, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 503
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 415
BseRI GAGGAG 3 cut(s) 103, 434, 438
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 2 cut(s) 234, 493
BshFI GGCC 3 cut(s) 394, 407, 511
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 2 cut(s) 113, 492
BsnI GGCC 3 cut(s) 394, 407, 511
Bso31I GGTCTC 2 cut(s) 113, 492
BsoBI CYCGRG 1 cut(s) 103
Bsp143I GATC 5 cut(s) 40, 226, 296, 334, 443
BspACI CCGC 7 cut(s) 237, 304, 342, 345, 416, 491, 493
BspANI GGCC 3 cut(s) 394, 407, 511
BspFNI CGCG 2 cut(s) 234, 493
BspLI GGNNCC 2 cut(s) 73, 510
BspPI GGATC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 362
BspTNI GGTCTC 2 cut(s) 113, 492
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 5 cut(s) 40, 226, 296, 334, 443
BssNI GRCGYC 2 cut(s) 183, 365
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 2 cut(s) 274, 461
Bst6I CTCTTC 1 cut(s) 362
BstACI GRCGYC 2 cut(s) 183, 365
BstDSI CCRYGG 1 cut(s) 491
BstF5I GGATG 1 cut(s) 117
BstFNI CGCG 2 cut(s) 234, 493
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 5 cut(s) 43, 229, 299, 337, 446
BstMAI GTCTC 2 cut(s) 113, 492
BstMBI GATC 5 cut(s) 40, 226, 296, 334, 443
BstMWI GCNNNNNNNGC 1 cut(s) 413
BstNI CCWGG 1 cut(s) 503
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 501
BstUI CGCG 2 cut(s) 234, 493
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 296, 443
BstYI RGATCY 2 cut(s) 296, 443
BsuRI GGCC 3 cut(s) 394, 407, 511
BtgI CCRYGG 1 cut(s) 491
BtsCI GGATG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 279
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 2 cut(s) 406, 509
Cfr42I CCGCGG 1 cut(s) 494
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 15
CviAII CATG 1 cut(s) 19
CviJI RGCY 8 cut(s) 47, 72, 79, 93, 166, 394, 407, 511
CviKI_1 RGCY 8 cut(s) 47, 72, 79, 93, 166, 394, 407, 511
CviQI GTAC 1 cut(s) 15
DpnI GATC 5 cut(s) 42, 228, 298, 336, 445
DpnII GATC 5 cut(s) 40, 226, 296, 334, 443
DraIII CACNNNGTG 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
EciI GGCGGA 1 cut(s) 431
Eco31I GGTCTC 2 cut(s) 113, 492
Eco57I CTGAAG 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 501
FaeI CATG 1 cut(s) 22
FaiI YATR 4 cut(s) 20, 39, 146, 326
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FatI CATG 1 cut(s) 18
FauI CCCGC 2 cut(s) 297, 349
FblI GTMKAC 3 cut(s) 99, 246, 456
Fnu4HI GCNGC 4 cut(s) 6, 237, 345, 491
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 4 cut(s) 6, 237, 345, 491
GlaI GCGC 1 cut(s) 235
GluI GCNGC 4 cut(s) 6, 237, 345, 491
HaeIII GGCC 3 cut(s) 394, 407, 511
HapII CCGG 1 cut(s) 395
HgaI GACGC 1 cut(s) 191
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 2 cut(s) 183, 365
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HincII GTYRAC 3 cut(s) 100, 247, 457
HindII GTYRAC 3 cut(s) 100, 247, 457
HinfI GANTC 4 cut(s) 84, 101, 126, 188
HpaII CCGG 1 cut(s) 395
Hpy166II GTNNAC 3 cut(s) 100, 247, 457
Hpy188I TCNGA 4 cut(s) 83, 214, 226, 312
Hpy188III TCNNGA 3 cut(s) 294, 361, 466
Hpy8I GTNNAC 3 cut(s) 100, 247, 457
Hpy99I CGWCG 6 cut(s) 234, 248, 367, 370, 461, 517
HpyCH4III ACNGT 2 cut(s) 274, 461
HpyCH4IV ACGT 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 413
HpySE526I ACGT 1 cut(s) 365
Hsp92I GRCGYC 2 cut(s) 183, 365
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 1 cut(s) 234
KspI CCGCGG 1 cut(s) 494
Kzo9I GATC 5 cut(s) 40, 226, 296, 334, 443
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 1 cut(s) 77
Lsp1109I GCAGC 1 cut(s) 17
MaeII ACGT 1 cut(s) 365
MalI GATC 5 cut(s) 42, 228, 298, 336, 445
MboI GATC 5 cut(s) 40, 226, 296, 334, 443
MboII GAAGA 2 cut(s) 62, 349
MflI RGATCY 2 cut(s) 296, 443
MluCI AATT 5 cut(s) 10, 62, 158, 256, 289
MlyI GAGTC 2 cut(s) 93, 95
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 9 cut(s) 124, 218, 358, 397, 412, 423, 429, 459, 499
MseI TTAA 2 cut(s) 255, 261
MspA1I CMGCKG 1 cut(s) 493
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 503
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 2 cut(s) 234, 493
MwoI GCNNNNNNNGC 1 cut(s) 413
NdeII GATC 5 cut(s) 40, 226, 296, 334, 443
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 2 cut(s) 73, 510
PciSI GCTCTTC 1 cut(s) 362
PfeI GAWTC 2 cut(s) 126, 188
PflFI GACNNNGTC 1 cut(s) 461
PkrI GCNGC 4 cut(s) 7, 238, 346, 492
PleI GAGTC 2 cut(s) 92, 95
PpsI GAGTC 2 cut(s) 92, 95
PshAI GACNNNNGTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 501
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 2 cut(s) 73, 510
PspPI GGNCC 2 cut(s) 406, 509
PsuI RGATCY 2 cut(s) 296, 443
PsyI GACNNNGTC 1 cut(s) 461
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
SacII CCGCGG 1 cut(s) 494
SalI GTCGAC 3 cut(s) 98, 245, 455
SapI GCTCTTC 1 cut(s) 362
SaqAI TTAA 2 cut(s) 255, 261
SatI GCNGC 4 cut(s) 6, 237, 345, 491
Sau3AI GATC 5 cut(s) 40, 226, 296, 334, 443
Sau96I GGNCC 2 cut(s) 406, 509
SchI GAGTC 2 cut(s) 93, 95
ScrFI CCNGG 1 cut(s) 503
SetI ASST 7 cut(s) 49, 81, 181, 210, 368, 434, 504
Sfr303I CCGCGG 1 cut(s) 494
SgrBI CCGCGG 1 cut(s) 494
Sse9I AATT 5 cut(s) 10, 62, 158, 256, 289
SsiI CCGC 7 cut(s) 237, 304, 342, 345, 416, 491, 493
StyD4I CCNGG 1 cut(s) 501
TaaI ACNGT 2 cut(s) 274, 461
TaiI ACGT 1 cut(s) 368
TaqI TCGA 5 cut(s) 43, 99, 246, 434, 456
TasI AATT 5 cut(s) 10, 62, 158, 256, 289
TauI GCSGC 3 cut(s) 239, 347, 493
TfiI GAWTC 2 cut(s) 126, 188
Tru1I TTAA 2 cut(s) 255, 261
Tru9I TTAA 2 cut(s) 255, 261
TscAI CASTG 1 cut(s) 279
TseI GCWGC 1 cut(s) 5
TspRI CASTG 1 cut(s) 279
Tth111I GACNNNGTC 1 cut(s) 461
XmiI GTMKAC 3 cut(s) 99, 246, 456
ZraI GACGTC 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.