Rh4DG099900

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0019492
Physical Location & Seq
Forward (+)
15203559 .. 15204089
531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG099900.1

Sequence Viewer

Length: 531 bp
ATGGCAGCAAATTGTACCATGATTGGCAAACAGGGTTATGATCGAGTTGAAGAAGTAAAGCAATTCGACGACTCCAAGCTCGGAGTCAAAGGCCTCGTCGACTCGGGTCTCTCCTCCATCCCCCGAATCTTTATCCACCCACCCATAATATCACATCAATTCCAGCCCACGACCCAACCCGACGCCGAATCAATCCCCACCATCAACCTCTCCAACCTCCACTCTGATCGTCGCTCCGCCATCGTCGACCCATTTAATTTTAACCATCAGACAGTGTTAGATTTGAACAATTCAAGATCCAAGCGGGTTCAGACAACACCCGACACAACAAACGATCTCCCCGCCGCCTCCTTGCTCTTCACGACGTCGTTCAGAACGTCGGTGTGGAGAAACTCAACCAGACGGCCGGAGTGGGAGGCCCACTGGGCGGAGGAGATGTTGAGGTCGAGGGAGAGATCTCCTCTGGTCAACGGCGTTAGGAAGTTGGTCTTGGCCTTGTTGCCGCGGAGAGACCTGGAGGGGCCGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

176

Amino Acids

19.9

Weight (kDa)

8.93

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019492)

Species Orthologous Gene IDs
rosa_laevigata RLG00000026769 RLG00000031276
rosa_multiflora Rmu_sc0000366.1_g000015 Rmu_sc0003863.1_g000012
rosa_samantha Rh4DG099900 Rh6BG445900 Rh6DG455900 Rh7AG260300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 368
AccI GTMKAC 2 cut(s) 99, 246
AccII CGCG 1 cut(s) 505
AciI CCGC 7 cut(s) 237, 304, 342, 345, 428, 503, 505
AclWI GGATC 1 cut(s) 291
AcoI YGGCCR 1 cut(s) 404
AcyI GRCGYC 2 cut(s) 183, 365
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 1 cut(s) 427
AgsI TTSAA 3 cut(s) 50, 286, 294
AjnI CCWGG 1 cut(s) 513
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AluBI AGCT 1 cut(s) 79
AluI AGCT 1 cut(s) 79
Alw26I GTCTC 2 cut(s) 113, 504
AlwI GGATC 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 103
AoxI GGCC 5 cut(s) 91, 404, 417, 492, 521
ApeKI GCWGC 1 cut(s) 5
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspS9I GGNCC 2 cut(s) 418, 521
AvaI CYCGRG 1 cut(s) 103
BbvI GCAGC 1 cut(s) 17
BccI CCATC 4 cut(s) 125, 209, 248, 273
BceAI ACGGC 3 cut(s) 419, 487, 508
BciT130I CCWGG 1 cut(s) 515
BcoDI GTCTC 2 cut(s) 113, 504
BglI GCCNNNNNGGC 1 cut(s) 425
BglII AGATCT 1 cut(s) 455
BisI GCNGC 3 cut(s) 6, 345, 503
BlsI GCNGC 3 cut(s) 7, 346, 504
Bme1390I CCNGG 1 cut(s) 515
BmeT110I CYCGRG 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 418, 521
BmiI GGNNCC 1 cut(s) 522
BmrFI CCNGG 1 cut(s) 515
BmrI ACTGGG 1 cut(s) 433
BmuI ACTGGG 1 cut(s) 433
BoxI GACNNNNGTC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 445, 477
BsaHI GRCGYC 2 cut(s) 183, 365
BsaI GGTCTC 2 cut(s) 113, 504
BsaJI CCNNGG 1 cut(s) 503
BsaXI ACNNNNNCTCC 3 cut(s) 422, 452, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse1I ACTGG 1 cut(s) 428
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 503
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 427
BseNI ACTGG 1 cut(s) 428
BseRI GAGGAG 3 cut(s) 103, 446, 450
BseX3I CGGCCG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 505
Bsh1285I CGRYCG 1 cut(s) 407
BshFI GGCC 5 cut(s) 93, 406, 419, 494, 523
BsiEI CGRYCG 1 cut(s) 407
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 1 cut(s) 407
BslI CCNNNNNNNGG 1 cut(s) 427
BsmAI GTCTC 2 cut(s) 113, 504
BsnI GGCC 5 cut(s) 93, 406, 419, 494, 523
Bso31I GGTCTC 2 cut(s) 113, 504
BsoBI CYCGRG 1 cut(s) 103
Bsp143I GATC 5 cut(s) 40, 226, 296, 334, 455
BspACI CCGC 7 cut(s) 237, 304, 342, 345, 428, 503, 505
BspANI GGCC 5 cut(s) 93, 406, 419, 494, 523
BspFNI CGCG 1 cut(s) 505
BspLI GGNNCC 1 cut(s) 522
BspPI GGATC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 362
BspTNI GGTCTC 2 cut(s) 113, 504
BsrI ACTGG 1 cut(s) 428
BssECI CCNNGG 1 cut(s) 503
BssMI GATC 5 cut(s) 40, 226, 296, 334, 455
BssNI GRCGYC 2 cut(s) 183, 365
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 1 cut(s) 362
BstACI GRCGYC 2 cut(s) 183, 365
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 1 cut(s) 117
BstFNI CGCG 1 cut(s) 505
BstKTI GATC 5 cut(s) 43, 229, 299, 337, 458
BstMAI GTCTC 2 cut(s) 113, 504
BstMBI GATC 5 cut(s) 40, 226, 296, 334, 455
BstMCI CGRYCG 1 cut(s) 407
BstMWI GCNNNNNNNGC 1 cut(s) 425
BstNI CCWGG 1 cut(s) 515
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 513
BstUI CGCG 1 cut(s) 505
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 296, 455
BstYI RGATCY 2 cut(s) 296, 455
BstZI CGGCCG 1 cut(s) 404
BsuRI GGCC 5 cut(s) 93, 406, 419, 494, 523
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 279, 421
Cfr13I GGNCC 2 cut(s) 418, 521
Cfr42I CCGCGG 1 cut(s) 506
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 15
CviAII CATG 1 cut(s) 19
CviJI RGCY 7 cut(s) 79, 93, 166, 406, 419, 494, 523
CviKI_1 RGCY 7 cut(s) 79, 93, 166, 406, 419, 494, 523
CviQI GTAC 1 cut(s) 15
DpnI GATC 5 cut(s) 42, 228, 298, 336, 457
DpnII GATC 5 cut(s) 40, 226, 296, 334, 455
EaeI YGGCCR 1 cut(s) 404
EagI CGGCCG 1 cut(s) 404
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
EciI GGCGGA 2 cut(s) 226, 443
EclXI CGGCCG 1 cut(s) 404
Eco147I AGGCCT 1 cut(s) 93
Eco31I GGTCTC 2 cut(s) 113, 504
Eco52I CGGCCG 1 cut(s) 404
Eco88I CYCGRG 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 513
FaeI CATG 1 cut(s) 22
FaiI YATR 4 cut(s) 20, 39, 146, 529
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FatI CATG 1 cut(s) 18
FauI CCCGC 2 cut(s) 297, 349
FblI GTMKAC 2 cut(s) 99, 246
Fnu4HI GCNGC 3 cut(s) 6, 345, 503
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 3 cut(s) 6, 345, 503
GluI GCNGC 3 cut(s) 6, 345, 503
HaeIII GGCC 5 cut(s) 93, 406, 419, 494, 523
HapII CCGG 1 cut(s) 407
HgaI GACGC 1 cut(s) 191
Hin1I GRCGYC 2 cut(s) 183, 365
Hin1II CATG 1 cut(s) 22
HincII GTYRAC 3 cut(s) 100, 247, 469
HindII GTYRAC 3 cut(s) 100, 247, 469
HinfI GANTC 5 cut(s) 71, 84, 101, 126, 188
HpaII CCGG 1 cut(s) 407
Hpy166II GTNNAC 3 cut(s) 100, 247, 469
Hpy188I TCNGA 5 cut(s) 83, 226, 270, 312, 374
Hpy188III TCNNGA 2 cut(s) 294, 361
Hpy8I GTNNAC 3 cut(s) 100, 247, 469
Hpy99I CGWCG 8 cut(s) 71, 101, 185, 234, 248, 367, 370, 382
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 2 cut(s) 365, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 425
HpySE526I ACGT 2 cut(s) 365, 377
Hsp92I GRCGYC 2 cut(s) 183, 365
Hsp92II CATG 1 cut(s) 22
KspI CCGCGG 1 cut(s) 506
Kzo9I GATC 5 cut(s) 40, 226, 296, 334, 455
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 8 cut(s) 17, 176, 409, 412, 420, 449, 500, 527
Lsp1109I GCAGC 1 cut(s) 17
MaeII ACGT 2 cut(s) 365, 377
MalI GATC 5 cut(s) 42, 228, 298, 336, 457
MboI GATC 5 cut(s) 40, 226, 296, 334, 455
MboII GAAGA 2 cut(s) 62, 349
MflI RGATCY 2 cut(s) 296, 455
MluCI AATT 5 cut(s) 10, 62, 158, 256, 289
MlyI GAGTC 3 cut(s) 65, 93, 95
MmeI TCCRAC 1 cut(s) 237
MseI TTAA 2 cut(s) 255, 261
MspA1I CMGCKG 1 cut(s) 505
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 515
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 1 cut(s) 505
MwoI GCNNNNNNNGC 1 cut(s) 425
NdeII GATC 5 cut(s) 40, 226, 296, 334, 455
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 522
PceI AGGCCT 1 cut(s) 93
PciSI GCTCTTC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 1 cut(s) 374
PfeI GAWTC 2 cut(s) 126, 188
PkrI GCNGC 3 cut(s) 7, 346, 504
PleI GAGTC 3 cut(s) 65, 92, 95
PpsI GAGTC 3 cut(s) 65, 92, 95
PshAI GACNNNNGTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 513
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 1 cut(s) 522
PspPI GGNCC 2 cut(s) 418, 521
PsuI RGATCY 2 cut(s) 296, 455
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
SacII CCGCGG 1 cut(s) 506
SalI GTCGAC 2 cut(s) 98, 245
SapI GCTCTTC 1 cut(s) 362
SaqAI TTAA 2 cut(s) 255, 261
SatI GCNGC 3 cut(s) 6, 345, 503
Sau3AI GATC 5 cut(s) 40, 226, 296, 334, 455
Sau96I GGNCC 2 cut(s) 418, 521
SchI GAGTC 3 cut(s) 65, 93, 95
ScrFI CCNGG 1 cut(s) 515
SetI ASST 7 cut(s) 81, 210, 219, 368, 380, 446, 516
Sfr303I CCGCGG 1 cut(s) 506
SgrBI CCGCGG 1 cut(s) 506
Sse9I AATT 5 cut(s) 10, 62, 158, 256, 289
SseBI AGGCCT 1 cut(s) 93
SsiI CCGC 7 cut(s) 237, 304, 342, 345, 428, 503, 505
StuI AGGCCT 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 513
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 2 cut(s) 368, 380
TaqI TCGA 5 cut(s) 43, 66, 99, 246, 446
TasI AATT 5 cut(s) 10, 62, 158, 256, 289
TauI GCSGC 2 cut(s) 347, 505
TfiI GAWTC 2 cut(s) 126, 188
Tru1I TTAA 2 cut(s) 255, 261
Tru9I TTAA 2 cut(s) 255, 261
TscAI CASTG 2 cut(s) 279, 428
TseI GCWGC 1 cut(s) 5
TspRI CASTG 2 cut(s) 279, 428
XmiI GTMKAC 2 cut(s) 99, 246
ZraI GACGTC 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.