Rmu_sc0004528.1_g000008

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004528.1
OG0019875
Physical Location & Seq
Forward (+)
37411 .. 41676
4266 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 405 bp
atgggtgagattagtgaagaaatgaggacaacagaagtggaaagcagagagtggtacatggctgcttatgcacctgaaagtgctccgacctccgatcatctcgaactccgcacaatcaaaatctcacttgcgtacgattcgatccctgaccaacatgttgtcctcgagaccctcttcctctcccttgatccgtacttgcgaagcaggatgactggccgagaagacggcctctgctctccccagtacaacctcaacgagtttattggccggctctggttatgttctagagcgaagcgcagacggcctggtggttggttcgagttggggtcacaggatcggaggtctcctgatggtgatgaccgggtcgcggcctcgttggaaacttctgggttcagatccaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

134

Amino Acids

15.35

Weight (kDa)

5.08

Isoelectric Point (pI)

76.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 17 - 77 7.5e-07 N-terminal domain of oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019875)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0105231
rosa_multiflora Rmu_sc0004528.1_g000008
rosa_roxburghii Rroxscaffold_2G00131210 Rroxscaffold_4G00315670 Rroxscaffold_6G00404010
rosa_samantha Rh2DG563800
rosa_wichuraiana Rw2G018290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 368
AciI CCGC 2 cut(s) 109, 368
AclWI GGATC 4 cut(s) 136, 182, 342, 390
AcoI YGGCCR 2 cut(s) 214, 265
AfaI GTAC 4 cut(s) 56, 134, 194, 245
AfiI CCNNNNNNNGG 1 cut(s) 367
AflIII ACRYGT 1 cut(s) 154
AjnI CCWGG 1 cut(s) 304
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 2 cut(s) 161, 348
AlwI GGATC 4 cut(s) 136, 182, 342, 390
Ama87I CYCGRG 1 cut(s) 164
AoxI GGCC 5 cut(s) 214, 226, 265, 302, 369
ApeKI GCWGC 1 cut(s) 62
AspLEI GCGC 1 cut(s) 297
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 2 cut(s) 17, 365
AvaI CYCGRG 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 1 cut(s) 49
BccI CCATC 1 cut(s) 344
BceAI ACGGC 2 cut(s) 241, 317
BciT130I CCWGG 1 cut(s) 306
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 161, 348
BfaI CTAG 1 cut(s) 285
BisI GCNGC 2 cut(s) 63, 369
BlsI GCNGC 2 cut(s) 64, 370
Bme1390I CCNGG 2 cut(s) 306, 362
BmeT110I CYCGRG 1 cut(s) 164
BmrFI CCNGG 2 cut(s) 306, 362
BmrI ACTGGG 1 cut(s) 235
BmuI ACTGGG 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 362
BsaI GGTCTC 2 cut(s) 161, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse118I RCCGGY 1 cut(s) 267
Bse1I ACTGG 2 cut(s) 217, 241
BseBI CCWGG 1 cut(s) 306
BseGI GGATG 1 cut(s) 213
BseLI CCNNNNNNNGG 1 cut(s) 367
BseNI ACTGG 2 cut(s) 217, 241
BseXI GCAGC 1 cut(s) 49
Bsh1236I CGCG 1 cut(s) 368
BshFI GGCC 5 cut(s) 216, 228, 267, 304, 371
BsiHKAI GWGCWC 1 cut(s) 85
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 2 cut(s) 268, 361
BsiWI CGTACG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 2 cut(s) 161, 348
BsnI GGCC 5 cut(s) 216, 228, 267, 304, 371
Bso31I GGTCTC 2 cut(s) 161, 348
BsoBI CYCGRG 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 5 cut(s) 94, 141, 187, 334, 395
BspACI CCGC 2 cut(s) 109, 368
BspANI GGCC 5 cut(s) 216, 228, 267, 304, 371
BspFNI CGCG 1 cut(s) 368
BspPI GGATC 4 cut(s) 136, 182, 342, 390
BspTNI GGTCTC 2 cut(s) 161, 348
BsrFI RCCGGY 1 cut(s) 267
BsrI ACTGG 2 cut(s) 217, 241
BssAI RCCGGY 1 cut(s) 267
BssMI GATC 5 cut(s) 94, 141, 187, 334, 395
Bst2UI CCWGG 1 cut(s) 306
Bst6I CTCTTC 1 cut(s) 179
BstC8I GCNNGC 1 cut(s) 269
BstF5I GGATG 1 cut(s) 213
BstFNI CGCG 1 cut(s) 368
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 5 cut(s) 97, 144, 190, 337, 398
BstMAI GTCTC 2 cut(s) 161, 348
BstMBI GATC 5 cut(s) 94, 141, 187, 334, 395
BstMWI GCNNNNNNNGC 2 cut(s) 68, 301
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 304, 360
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
BsuRI GGCC 5 cut(s) 216, 228, 267, 304, 371
BtsCI GGATG 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 269
CfoI GCGC 1 cut(s) 297
Cfr10I RCCGGY 1 cut(s) 267
Csp6I GTAC 4 cut(s) 55, 133, 193, 244
CspCI CAANNNNNGTGG 2 cut(s) 18, 53
CviAII CATG 2 cut(s) 58, 155
CviJI RGCY 7 cut(s) 62, 216, 228, 267, 271, 304, 371
CviKI_1 RGCY 7 cut(s) 62, 216, 228, 267, 271, 304, 371
CviQI GTAC 4 cut(s) 55, 133, 193, 244
DpnI GATC 5 cut(s) 96, 143, 189, 336, 397
DpnII GATC 5 cut(s) 94, 141, 187, 334, 395
EaeI YGGCCR 2 cut(s) 214, 265
Eam1104I CTCTTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 179
Eco31I GGTCTC 2 cut(s) 161, 348
Eco88I CYCGRG 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 304
FaeI CATG 2 cut(s) 61, 158
FaiI YATR 4 cut(s) 59, 69, 156, 280
FatI CATG 2 cut(s) 57, 154
Fnu4HI GCNGC 2 cut(s) 63, 369
FokI GGATG 1 cut(s) 220
Fsp4HI GCNGC 2 cut(s) 63, 369
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 296
GluI GCNGC 2 cut(s) 63, 369
HaeIII GGCC 5 cut(s) 216, 228, 267, 304, 371
HapII CCGG 2 cut(s) 268, 361
HhaI GCGC 1 cut(s) 297
Hin1II CATG 2 cut(s) 61, 158
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HinfI GANTC 1 cut(s) 137
HpaII CCGG 2 cut(s) 268, 361
HphI GGTGA 2 cut(s) 17, 365
Hpy188I TCNGA 4 cut(s) 87, 94, 339, 395
Hpy188III TCNNGA 4 cut(s) 101, 166, 285, 347
HpyCH4V TGCA 1 cut(s) 71
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 301
Hsp92II CATG 2 cut(s) 61, 158
HspAI GCGC 1 cut(s) 295
KroI GCCGGC 1 cut(s) 267
KroNI GCCGGC 1 cut(s) 269
Kzo9I GATC 5 cut(s) 94, 141, 187, 334, 395
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 1 cut(s) 49
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 327
MalI GATC 5 cut(s) 96, 143, 189, 336, 397
MboI GATC 5 cut(s) 94, 141, 187, 334, 395
MboII GAAGA 3 cut(s) 29, 166, 233
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 1 cut(s) 400
MmeI TCCRAC 2 cut(s) 110, 357
MnlI CCTC 9 cut(s) 18, 100, 173, 182, 188, 239, 260, 333, 382
MroNI GCCGGC 1 cut(s) 267
MspI CCGG 2 cut(s) 268, 361
MspR9I CCNGG 2 cut(s) 306, 362
MvaI CCWGG 1 cut(s) 306
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 68, 301
NaeI GCCGGC 1 cut(s) 269
NciI CCSGG 1 cut(s) 362
NdeII GATC 5 cut(s) 94, 141, 187, 334, 395
NgoMIV GCCGGC 1 cut(s) 267
NlaIII CATG 2 cut(s) 61, 158
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 1 cut(s) 327
NspI RCATGY 1 cut(s) 158
PaeR7I CTCGAG 1 cut(s) 164
PciI ACATGT 1 cut(s) 154
PdiI GCCGGC 1 cut(s) 269
PfeI GAWTC 1 cut(s) 137
Pfl23II CGTACG 1 cut(s) 132
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 2 cut(s) 64, 370
PscI ACATGT 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspLI CGTACG 1 cut(s) 132
PsuI RGATCY 1 cut(s) 395
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 4 cut(s) 56, 134, 194, 245
RsaNI GTAC 4 cut(s) 55, 133, 193, 244
SatI GCNGC 2 cut(s) 63, 369
Sau3AI GATC 5 cut(s) 94, 141, 187, 334, 395
ScrFI CCNGG 2 cut(s) 306, 362
SduI GDGCHC 1 cut(s) 85
SetI ASST 4 cut(s) 76, 92, 252, 344
Sfr274I CTCGAG 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 1 cut(s) 400
SsiI CCGC 2 cut(s) 109, 368
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 304, 360
TaqI TCGA 4 cut(s) 102, 140, 165, 318
TasI AATT 1 cut(s) 400
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 1 cut(s) 137
TseFI GTSAC 1 cut(s) 327
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 327
TspGWI ACGGA 1 cut(s) 180
Tth111I GACNNNGTC 1 cut(s) 362
XbaI TCTAGA 1 cut(s) 284
XceI RCATGY 1 cut(s) 158
XhoI CTCGAG 1 cut(s) 164
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.