Rroxscaffold_4G00315670

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0019875
Physical Location & Seq
Forward (+)
40100843 .. 40101238
396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00315670.1

Sequence Viewer

Length: 396 bp
ATGCCCGGTCCGACGCCGCCTTGCGGAGGCGGCGTCGGACGGGCTCGAAGGGTCCTTGTGGCCGGGATTGCATGGTCTTGTTGGGGAGAAGGCTACGGGATGGCGACATTGGAGTTTGGAGGATTTAACGGCGAAGGGCGGCGGCGGGTTGATGGATGGATGTCGGGGATGATGTCGCCCGACCATAGGACGGAGTGCGGATGTTTTGCAGCAATGTGGTGGGATCGGAGATCGCCCGAAGGTGGTGACCTGGACAACGGCGTTGCTGCGATTCACCGGTGGCGGATCGGATCGGTTGGACTTGATCCTATCGGATCGGATCAATCACTTGGCGGGATGCTTTGGCTCCGATCTCCGGAGGGTGGGGTGGCTAGCGAGGAAGGTGGATGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

14.01

Weight (kDa)

5.3

Isoelectric Point (pI)

84.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019875)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0105231
rosa_multiflora Rmu_sc0004528.1_g000008
rosa_roxburghii Rroxscaffold_2G00131210 Rroxscaffold_4G00315670 Rroxscaffold_6G00404010
rosa_samantha Rh2DG563800
rosa_wichuraiana Rw2G018290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 9 cut(s) 17, 24, 30, 139, 142, 145, 198, 283, 333
AclWI GGATC 6 cut(s) 231, 293, 298, 299, 322, 327
AcoI YGGCCR 1 cut(s) 60
AcyI GRCGYC 2 cut(s) 14, 33
AfiI CCNNNNNNNGG 7 cut(s) 23, 26, 186, 190, 242, 355, 362
AgeI ACCGGT 1 cut(s) 276
AjnI CCWGG 1 cut(s) 249
AlwI GGATC 6 cut(s) 231, 293, 298, 299, 322, 327
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 2 cut(s) 209, 266
AsiGI ACCGGT 1 cut(s) 276
AspS9I GGNCC 2 cut(s) 8, 52
AsuC2I CCSGG 2 cut(s) 6, 64
AsuHPI GGTGA 2 cut(s) 257, 266
AsuNHI GCTAGC 1 cut(s) 371
AvaII GGWCC 2 cut(s) 8, 52
BanII GRGCYC 1 cut(s) 46
BbvI GCAGC 2 cut(s) 221, 253
BccI CCATC 4 cut(s) 94, 146, 150, 381
BceAI ACGGC 2 cut(s) 145, 274
BciT130I CCWGG 1 cut(s) 251
BcnI CCSGG 2 cut(s) 6, 64
BfaI CTAG 1 cut(s) 372
BisI GCNGC 6 cut(s) 17, 31, 140, 143, 210, 267
BlsI GCNGC 6 cut(s) 18, 32, 141, 144, 211, 268
Bme1390I CCNGG 3 cut(s) 6, 64, 251
Bme18I GGWCC 2 cut(s) 8, 52
BmgT120I GGNCC 2 cut(s) 8, 52
BmiI GGNNCC 2 cut(s) 53, 347
BmrFI CCNGG 3 cut(s) 6, 64, 251
BmsI GCATC 1 cut(s) 327
BmtI GCTAGC 1 cut(s) 375
BpuMI CCSGG 2 cut(s) 6, 64
BsaHI GRCGYC 2 cut(s) 14, 33
BsaWI WCCGGW 2 cut(s) 276, 355
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
Bsc4I CCNNNNNNNGG 7 cut(s) 23, 26, 186, 190, 242, 355, 362
Bse118I RCCGGY 1 cut(s) 276
Bse3DI GCAATG 1 cut(s) 219
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 1 cut(s) 251
BseGI GGATG 7 cut(s) 105, 161, 165, 174, 206, 342, 392
BseLI CCNNNNNNNGG 7 cut(s) 23, 26, 186, 190, 242, 355, 362
BseMI GCAATG 1 cut(s) 219
BseXI GCAGC 2 cut(s) 221, 253
BshFI GGCC 1 cut(s) 62
BshTI ACCGGT 1 cut(s) 276
BsiSI CCGG 4 cut(s) 6, 63, 277, 356
BslI CCNNNNNNNGG 7 cut(s) 23, 26, 186, 190, 242, 355, 362
BsnI GGCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 46
Bsp13I TCCGGA 1 cut(s) 355
Bsp143I GATC 8 cut(s) 223, 230, 285, 290, 304, 314, 319, 350
BspACI CCGC 9 cut(s) 17, 24, 30, 139, 142, 145, 198, 283, 333
BspANI GGCC 1 cut(s) 62
BspEI TCCGGA 1 cut(s) 355
BspLI GGNNCC 2 cut(s) 53, 347
BspOI GCTAGC 1 cut(s) 375
BspPI GGATC 6 cut(s) 231, 293, 298, 299, 322, 327
BsrDI GCAATG 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 276
BssAI RCCGGY 1 cut(s) 276
BssMI GATC 8 cut(s) 223, 230, 285, 290, 304, 314, 319, 350
BssNI GRCGYC 2 cut(s) 14, 33
Bst2UI CCWGG 1 cut(s) 251
BstACI GRCGYC 2 cut(s) 14, 33
BstC8I GCNNGC 1 cut(s) 373
BstEII GGTNACC 1 cut(s) 245
BstENI CCTNNNNNAGG 1 cut(s) 24
BstF5I GGATG 7 cut(s) 105, 161, 165, 174, 206, 342, 392
BstKTI GATC 8 cut(s) 226, 233, 288, 293, 307, 317, 322, 353
BstMBI GATC 8 cut(s) 223, 230, 285, 290, 304, 314, 319, 350
BstMWI GCNNNNNNNGC 2 cut(s) 30, 68
BstNI CCWGG 1 cut(s) 251
BstPI GGTNACC 1 cut(s) 245
BstSCI CCNGG 3 cut(s) 4, 62, 249
BstV1I GCAGC 2 cut(s) 221, 253
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 7 cut(s) 105, 161, 165, 174, 206, 342, 392
Cac8I GCNNGC 1 cut(s) 373
Cfr10I RCCGGY 1 cut(s) 276
Cfr13I GGNCC 2 cut(s) 8, 52
CpoI CGGWCCG 1 cut(s) 8
CseI GACGC 2 cut(s) 22, 22
CspAI ACCGGT 1 cut(s) 276
CspI CGGWCCG 1 cut(s) 8
CviAII CATG 1 cut(s) 72
CviJI RGCY 5 cut(s) 44, 62, 93, 346, 371
CviKI_1 RGCY 5 cut(s) 44, 62, 93, 346, 371
DpnI GATC 8 cut(s) 225, 232, 287, 292, 306, 316, 321, 352
DpnII GATC 8 cut(s) 223, 230, 285, 290, 304, 314, 319, 350
EaeI YGGCCR 1 cut(s) 60
EciI GGCGGA 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 46
Eco47I GGWCC 2 cut(s) 8, 52
Eco91I GGTNACC 1 cut(s) 245
EcoNI CCTNNNNNAGG 1 cut(s) 24
EcoO109I RGGNCCY 1 cut(s) 52
EcoO65I GGTNACC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 46
FaeI CATG 1 cut(s) 75
FaiI YATR 2 cut(s) 73, 186
FatI CATG 1 cut(s) 71
FauI CCCGC 2 cut(s) 138, 326
Fnu4HI GCNGC 6 cut(s) 17, 31, 140, 143, 210, 267
FokI GGATG 6 cut(s) 112, 168, 172, 181, 213, 349
FriOI GRGCYC 1 cut(s) 46
Fsp4HI GCNGC 6 cut(s) 17, 31, 140, 143, 210, 267
FspBI CTAG 1 cut(s) 372
GluI GCNGC 6 cut(s) 17, 31, 140, 143, 210, 267
HaeIII GGCC 1 cut(s) 62
HapII CCGG 4 cut(s) 6, 63, 277, 356
HgaI GACGC 2 cut(s) 22, 22
Hin1I GRCGYC 2 cut(s) 14, 33
Hin1II CATG 1 cut(s) 75
HinfI GANTC 1 cut(s) 271
HpaII CCGG 4 cut(s) 6, 63, 277, 356
HphI GGTGA 2 cut(s) 257, 266
Hpy188I TCNGA 7 cut(s) 12, 38, 228, 290, 314, 319, 350
Hpy188III TCNNGA 1 cut(s) 356
Hpy99I CGWCG 2 cut(s) 16, 38
HpyAV CCTTC 5 cut(s) 42, 83, 128, 233, 374
HpyCH4V TGCA 2 cut(s) 71, 209
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 68
Hsp92I GRCGYC 2 cut(s) 14, 33
Hsp92II CATG 1 cut(s) 75
Kpn2I TCCGGA 1 cut(s) 355
Kzo9I GATC 8 cut(s) 223, 230, 285, 290, 304, 314, 319, 350
LmnI GCTCC 1 cut(s) 351
LpnPI CCDG 6 cut(s) 19, 76, 236, 263, 290, 369
Lsp1109I GCAGC 2 cut(s) 221, 253
LweI GCATC 1 cut(s) 327
MaeI CTAG 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 245
MalI GATC 8 cut(s) 225, 232, 287, 292, 306, 316, 321, 352
MboI GATC 8 cut(s) 223, 230, 285, 290, 304, 314, 319, 350
MhlI GDGCHC 1 cut(s) 46
MmeI TCCRAC 3 cut(s) 16, 35, 277
MnlI CCTC 4 cut(s) 20, 113, 352, 370
MroI TCCGGA 1 cut(s) 355
MseI TTAA 1 cut(s) 126
MspI CCGG 4 cut(s) 6, 63, 277, 356
MspR9I CCNGG 3 cut(s) 6, 64, 251
MvaI CCWGG 1 cut(s) 251
MwoI GCNNNNNNNGC 2 cut(s) 30, 68
NciI CCSGG 2 cut(s) 6, 64
NdeII GATC 8 cut(s) 223, 230, 285, 290, 304, 314, 319, 350
NheI GCTAGC 1 cut(s) 371
NlaIII CATG 1 cut(s) 75
NlaIV GGNNCC 2 cut(s) 53, 347
NmuCI GTSAC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 271
PinAI ACCGGT 1 cut(s) 276
PkrI GCNGC 6 cut(s) 18, 32, 141, 144, 211, 268
PpuMI RGGWCCY 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 249
PspEI GGTNACC 1 cut(s) 245
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 2 cut(s) 53, 347
PspPI GGNCC 2 cut(s) 8, 52
PspPPI RGGWCCY 1 cut(s) 52
Rsr2I CGGWCCG 1 cut(s) 8
RsrII CGGWCCG 1 cut(s) 8
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 6 cut(s) 17, 31, 140, 143, 210, 267
Sau3AI GATC 8 cut(s) 223, 230, 285, 290, 304, 314, 319, 350
Sau96I GGNCC 2 cut(s) 8, 52
ScrFI CCNGG 3 cut(s) 6, 64, 251
SduI GDGCHC 1 cut(s) 46
SetI ASST 3 cut(s) 244, 252, 385
SfaNI GCATC 1 cut(s) 327
SgrAI CRCCGGYG 1 cut(s) 276
SinI GGWCC 2 cut(s) 8, 52
SsiI CCGC 9 cut(s) 17, 24, 30, 139, 142, 145, 198, 283, 333
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 3 cut(s) 4, 62, 249
TaqI TCGA 1 cut(s) 46
TauI GCSGC 4 cut(s) 19, 33, 142, 145
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 2 cut(s) 209, 266
Tsp45I GTSAC 1 cut(s) 245
TspGWI ACGGA 1 cut(s) 206
VpaK11BI GGWCC 2 cut(s) 8, 52
XagI CCTNNNNNAGG 1 cut(s) 24
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.