Rroxscaffold_6G00404010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0019875
Physical Location & Seq
Reverse (-)
26445832 .. 26453748
7917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404010.1

Sequence Viewer

Length: 549 bp
ATGGCGCGGGGACTCGTCGCAACGGATGTGGCTGTTTGCGGCGCTTCGTCTTGGGGGGAGACGGGTGTTGGACCGGGCCAATCCATCGGCCACATGAACATGATTCACATTGACATTGGACTCGACGACTTGCTATTACCGGACCATGATTCACGCGGCGAGAAGTCTTCGCTGCAGTTTATGCCCGATCCGACGCACGCCCTCGCGGTGGCATCATCGGAAGGGCTAGAAGGGTCCTTGTGTCCAGGATTGCATGGTCCGGATTGGGAAGAAGGCTGCGAATGGCAACGTTGGCGTTTGGAGGATTTCACGGCAAAGGGAGCGGCGGGTTGGTGGATGGGTATCGGGCCGATGTCGCCTAACCATAGGAAGGAGTGCGGATGTTTTGCTGCGGTGTGGTGGGATAGGAGATCGCCCGAGGGTGGTGACCGGACGACGTTGTCGCTGCGATTCTCGGGTGGCGGATCCGATCGGTTGGACTCGATCCCATCGATCGATCGATCACTTGGTGGGATGTTCGGCTCCGATCTCGAGGGGTGGAGTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

19.49

Weight (kDa)

4.74

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019875)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0105231
rosa_multiflora Rmu_sc0004528.1_g000008
rosa_roxburghii Rroxscaffold_2G00131210 Rroxscaffold_4G00315670 Rroxscaffold_6G00404010
rosa_samantha Rh2DG563800
rosa_wichuraiana Rw2G018290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 439
AccBSI CCGCTC 1 cut(s) 323
AccII CGCG 3 cut(s) 7, 156, 206
AccIII TCCGGA 1 cut(s) 259
AciI CCGC 9 cut(s) 7, 39, 156, 206, 323, 326, 378, 392, 462
AclI AACGTT 1 cut(s) 289
AclWI GGATC 4 cut(s) 182, 459, 472, 478
AcoI YGGCCR 1 cut(s) 88
AdeI CACNNNGTG 1 cut(s) 509
AfiI CCNNNNNNNGG 3 cut(s) 208, 370, 422
AjnI CCWGG 1 cut(s) 244
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 4 cut(s) 182, 459, 472, 478
Ama87I CYCGRG 3 cut(s) 416, 454, 530
Aor13HI TCCGGA 1 cut(s) 259
AoxI GGCC 3 cut(s) 76, 88, 347
ApeKI GCWGC 4 cut(s) 172, 276, 389, 445
AspLEI GCGC 2 cut(s) 7, 44
AspS9I GGNCC 6 cut(s) 71, 76, 142, 234, 257, 347
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 437
AvaI CYCGRG 3 cut(s) 416, 454, 530
AvaII GGWCC 4 cut(s) 71, 142, 234, 257
BamHI GGATCC 1 cut(s) 464
BbsI GAAGAC 1 cut(s) 159
BbvI GCAGC 4 cut(s) 159, 263, 376, 432
BccI CCATC 3 cut(s) 92, 331, 496
BceAI ACGGC 1 cut(s) 327
BciT130I CCWGG 1 cut(s) 246
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 2 cut(s) 227, 547
BfmI CTRYAG 1 cut(s) 173
BfoI RGCGCY 1 cut(s) 45
BisI GCNGC 7 cut(s) 40, 157, 173, 277, 324, 390, 446
BlsI GCNGC 7 cut(s) 41, 158, 174, 278, 325, 391, 447
Bme1390I CCNGG 2 cut(s) 75, 246
Bme18I GGWCC 4 cut(s) 71, 142, 234, 257
BmeT110I CYCGRG 3 cut(s) 416, 454, 530
BmgT120I GGNCC 6 cut(s) 71, 76, 142, 234, 257, 347
BmiI GGNNCC 3 cut(s) 235, 466, 523
BmrFI CCNGG 2 cut(s) 75, 246
BmsI GCATC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 159
BpuMI CCSGG 1 cut(s) 75
Bsa29I ATCGAT 3 cut(s) 491, 495, 499
BsaBI GATNNNNATC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 417
BsaWI WCCGGW 3 cut(s) 139, 259, 429
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 370, 422
Bse8I GATNNNNATC 1 cut(s) 341
BseAI TCCGGA 1 cut(s) 259
BseBI CCWGG 1 cut(s) 246
BseCI ATCGAT 3 cut(s) 491, 495, 499
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 4 cut(s) 31, 342, 386, 519
BseJI GATNNNNATC 1 cut(s) 341
BseLI CCNNNNNNNGG 3 cut(s) 208, 370, 422
BseXI GCAGC 4 cut(s) 159, 263, 376, 432
Bsh1236I CGCG 3 cut(s) 7, 156, 206
Bsh1285I CGRYCG 3 cut(s) 472, 495, 499
BshFI GGCC 3 cut(s) 78, 90, 349
BshVI ATCGAT 3 cut(s) 491, 495, 499
BsiEI CGRYCG 3 cut(s) 472, 495, 499
BsiHKCI CYCGRG 3 cut(s) 416, 454, 530
BsiSI CCGG 4 cut(s) 74, 140, 260, 430
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 3 cut(s) 208, 370, 422
BsmAI GTCTC 1 cut(s) 53
BsmBI CGTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 3 cut(s) 78, 90, 349
BsoBI CYCGRG 3 cut(s) 416, 454, 530
Bsp13I TCCGGA 1 cut(s) 259
Bsp143I GATC 9 cut(s) 187, 410, 464, 469, 483, 492, 496, 500, 526
BspACI CCGC 9 cut(s) 7, 39, 156, 206, 323, 326, 378, 392, 462
BspANI GGCC 3 cut(s) 78, 90, 349
BspDI ATCGAT 3 cut(s) 491, 495, 499
BspEI TCCGGA 1 cut(s) 259
BspFNI CGCG 3 cut(s) 7, 156, 206
BspLI GGNNCC 3 cut(s) 235, 466, 523
BspMAI CTGCAG 1 cut(s) 177
BspPI GGATC 4 cut(s) 182, 459, 472, 478
BsrBI CCGCTC 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 9 cut(s) 187, 410, 464, 469, 483, 492, 496, 500, 526
Bst2UI CCWGG 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 198
BstEII GGTNACC 1 cut(s) 425
BstF5I GGATG 4 cut(s) 31, 342, 386, 519
BstFNI CGCG 3 cut(s) 7, 156, 206
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 2 cut(s) 7, 44
BstKTI GATC 9 cut(s) 190, 413, 467, 472, 486, 495, 499, 503, 529
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 9 cut(s) 187, 410, 464, 469, 483, 492, 496, 500, 526
BstMCI CGRYCG 3 cut(s) 472, 495, 499
BstMWI GCNNNNNNNGC 4 cut(s) 181, 292, 320, 355
BstNI CCWGG 1 cut(s) 246
BstPI GGTNACC 1 cut(s) 425
BstSCI CCNGG 2 cut(s) 73, 244
BstSFI CTRYAG 1 cut(s) 173
BstUI CGCG 3 cut(s) 7, 156, 206
BstV1I GCAGC 4 cut(s) 159, 263, 376, 432
BstV2I GAAGAC 1 cut(s) 159
BstX2I RGATCY 1 cut(s) 464
BstYI RGATCY 1 cut(s) 464
Bsu15I ATCGAT 3 cut(s) 491, 495, 499
BsuRI GGCC 3 cut(s) 78, 90, 349
BsuTUI ATCGAT 3 cut(s) 491, 495, 499
BtsCI GGATG 4 cut(s) 31, 342, 386, 519
Cac8I GCNNGC 1 cut(s) 198
CfoI GCGC 2 cut(s) 7, 44
Cfr13I GGNCC 6 cut(s) 71, 76, 142, 234, 257, 347
ClaI ATCGAT 3 cut(s) 491, 495, 499
CseI GACGC 1 cut(s) 202
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviAII CATG 4 cut(s) 94, 100, 146, 254
CviJI RGCY 8 cut(s) 32, 78, 90, 226, 276, 349, 522, 546
CviKI_1 RGCY 8 cut(s) 32, 78, 90, 226, 276, 349, 522, 546
DpnI GATC 9 cut(s) 189, 412, 466, 471, 485, 494, 498, 502, 528
DpnII GATC 9 cut(s) 187, 410, 464, 469, 483, 492, 496, 500, 526
DraIII CACNNNGTG 1 cut(s) 509
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 88
EciI GGCGGA 1 cut(s) 477
Eco47I GGWCC 4 cut(s) 71, 142, 234, 257
Eco88I CYCGRG 3 cut(s) 416, 454, 530
Eco91I GGTNACC 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 234
EcoO65I GGTNACC 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 244
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 4 cut(s) 97, 103, 149, 257
FaiI YATR 6 cut(s) 95, 101, 147, 182, 255, 366
FaqI GGGAC 1 cut(s) 24
FatI CATG 4 cut(s) 93, 99, 145, 253
FauI CCCGC 1 cut(s) 319
Fnu4HI GCNGC 7 cut(s) 40, 157, 173, 277, 324, 390, 446
FokI GGATG 4 cut(s) 38, 349, 393, 526
Fsp4HI GCNGC 7 cut(s) 40, 157, 173, 277, 324, 390, 446
FspBI CTAG 2 cut(s) 227, 547
GlaI GCGC 2 cut(s) 6, 43
GluI GCNGC 7 cut(s) 40, 157, 173, 277, 324, 390, 446
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 3 cut(s) 78, 90, 349
HapII CCGG 4 cut(s) 74, 140, 260, 430
HgaI GACGC 1 cut(s) 202
HhaI GCGC 2 cut(s) 7, 44
Hin1II CATG 4 cut(s) 97, 103, 149, 257
Hin6I GCGC 2 cut(s) 5, 42
HinP1I GCGC 2 cut(s) 5, 42
HinfI GANTC 6 cut(s) 12, 103, 120, 149, 450, 479
HpaII CCGG 4 cut(s) 74, 140, 260, 430
HphI GGTGA 1 cut(s) 437
Hpy188I TCNGA 4 cut(s) 192, 220, 469, 526
Hpy188III TCNNGA 2 cut(s) 260, 530
Hpy99I CGWCG 4 cut(s) 20, 128, 196, 439
HpyAV CCTTC 4 cut(s) 215, 224, 266, 364
HpyCH4IV ACGT 2 cut(s) 289, 437
HpyCH4V TGCA 2 cut(s) 175, 253
HpyF10VI GCNNNNNNNGC 4 cut(s) 181, 292, 320, 355
HpySE526I ACGT 2 cut(s) 289, 437
Hsp92II CATG 4 cut(s) 97, 103, 149, 257
HspAI GCGC 2 cut(s) 5, 42
Kpn2I TCCGGA 1 cut(s) 259
Kzo9I GATC 9 cut(s) 187, 410, 464, 469, 483, 492, 496, 500, 526
LmnI GCTCC 2 cut(s) 320, 527
LpnPI CCDG 6 cut(s) 87, 153, 231, 258, 273, 443
Lsp1109I GCAGC 4 cut(s) 159, 263, 376, 432
LweI GCATC 1 cut(s) 221
MaeI CTAG 2 cut(s) 227, 547
MaeII ACGT 2 cut(s) 289, 437
MaeIII GTNAC 1 cut(s) 425
MalI GATC 9 cut(s) 189, 412, 466, 471, 485, 494, 498, 502, 528
MbiI CCGCTC 1 cut(s) 323
MboI GATC 9 cut(s) 187, 410, 464, 469, 483, 492, 496, 500, 526
MboII GAAGA 2 cut(s) 159, 281
MflI RGATCY 1 cut(s) 464
MlyI GAGTC 3 cut(s) 6, 114, 473
MmeI TCCRAC 3 cut(s) 49, 215, 456
MnlI CCTC 4 cut(s) 212, 295, 412, 526
MroI TCCGGA 1 cut(s) 259
MslI CAYNNNNRTG 1 cut(s) 98
MspI CCGG 4 cut(s) 74, 140, 260, 430
MspR9I CCNGG 2 cut(s) 75, 246
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 3 cut(s) 7, 156, 206
MwoI GCNNNNNNNGC 4 cut(s) 181, 292, 320, 355
NciI CCSGG 1 cut(s) 75
NdeII GATC 9 cut(s) 187, 410, 464, 469, 483, 492, 496, 500, 526
NlaIII CATG 4 cut(s) 97, 103, 149, 257
NlaIV GGNNCC 3 cut(s) 235, 466, 523
NmuCI GTSAC 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 530
PcsI WCGNNNNNNNCGW 1 cut(s) 488
PfeI GAWTC 3 cut(s) 103, 149, 450
PflFI GACNNNGTC 1 cut(s) 439
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 7 cut(s) 41, 158, 174, 278, 325, 391, 447
Ple19I CGATCG 3 cut(s) 472, 495, 499
PleI GAGTC 3 cut(s) 6, 114, 473
PpsI GAGTC 3 cut(s) 6, 114, 473
PpuMI RGGWCCY 1 cut(s) 234
Psp1406I AACGTT 1 cut(s) 289
Psp5II RGGWCCY 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 244
PspEI GGTNACC 1 cut(s) 425
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 3 cut(s) 235, 466, 523
PspPI GGNCC 6 cut(s) 71, 76, 142, 234, 257, 347
PspPPI RGGWCCY 1 cut(s) 234
PstI CTGCAG 1 cut(s) 177
PsuI RGATCY 1 cut(s) 464
PsyI GACNNNGTC 1 cut(s) 439
PvuI CGATCG 3 cut(s) 472, 495, 499
RseI CAYNNNNRTG 1 cut(s) 98
SatI GCNGC 7 cut(s) 40, 157, 173, 277, 324, 390, 446
Sau3AI GATC 9 cut(s) 187, 410, 464, 469, 483, 492, 496, 500, 526
Sau96I GGNCC 6 cut(s) 71, 76, 142, 234, 257, 347
SchI GAGTC 3 cut(s) 6, 114, 473
ScrFI CCNGG 2 cut(s) 75, 246
SetI ASST 2 cut(s) 292, 440
SfaNI GCATC 1 cut(s) 221
SfcI CTRYAG 1 cut(s) 173
Sfr274I CTCGAG 1 cut(s) 530
SinI GGWCC 4 cut(s) 71, 142, 234, 257
SlaI CTCGAG 1 cut(s) 530
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SsiI CCGC 9 cut(s) 7, 39, 156, 206, 323, 326, 378, 392, 462
SspMI CTAG 2 cut(s) 227, 547
StyD4I CCNGG 2 cut(s) 73, 244
TaiI ACGT 2 cut(s) 292, 440
TaqI TCGA 6 cut(s) 123, 482, 491, 495, 499, 531
TauI GCSGC 3 cut(s) 42, 159, 326
TfiI GAWTC 3 cut(s) 103, 149, 450
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 4 cut(s) 172, 276, 389, 445
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 1 cut(s) 110
TspGWI ACGGA 1 cut(s) 38
Tth111I GACNNNGTC 1 cut(s) 439
VpaK11BI GGWCC 4 cut(s) 71, 142, 234, 257
XhoI CTCGAG 1 cut(s) 530
XspI CTAG 2 cut(s) 227, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.