Rroxscaffold_2G00131210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019875
Physical Location & Seq
Reverse (-)
67742060 .. 67766652
24593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131210.1

Sequence Viewer

Length: 984 bp
ATGTTGATGCAGTTTGCCCCAAAGAGAAAAGCTAGCGATGTATGTGCAGCTGCAGTCCATGATTTTGGAGGTGACCAAACCTACTGTGGACTTGGTAATATTAGAAGGGCTACTAGCAGGGTTCAAGGCAAGTTTGATGTGCTTTTTATGTGGCCATGTGATTACTCATGCACAACAGAGGTCCAGCAAGCAATTCATGTTTTGAAGAAAGCATATGGTAGTCTTGAACCCGCCAATATCCTCAATACTCTTCTTGGAAATGATGATGATGGAGTGAAGAGACCTAACGTTGGAAGGAAAAAATTGGATTGCTATGTGGTGGAACCATTTTCTAATGCGAGTATTGTGTCAAAGGCGTCGGTCTTCCGTCAAAATTTATTGCCCGGTCCAACCGCACACCCGGTGGCGGCGTCGGCATTGGACAAAGCCGAATTGGAACTTGTGTCCGGTGTTGTAGGGTCTAGTAGGAGAGAAGGCAACAGGGTGGCGACGTTTTCCAAGGCGTTTGGAGTACTTAACGACGATGTGCAGCGGCGGGTTGATGGATGGGAGTCGGGGCCGATGTCGCCTAGCCATTGGAAGGAGCGCAGACGAATGGAGGGAAACACTCAGCACCTTACACCTGCAGCTTCGATTGGTTTTGAATCAATGTGTTCAGATCAGCATTCTACCGTCTCATGTACTTGGGGATCTGCAAAGTCCCGCTCCAATGAAATAGGCAAGGAATATGACCTTATATTGCTCGGTAAAAACAACCACCGAAGCACTAGCACTAAGGATTCAAAAGTTAAATGCTTACACCATGAACATCTTGCATCCACCCAGGAACACTTTCGAAAGAAGAGTGATGCACCGCTCCATTGCTACCTCTCGCACTCATGTTGCCAAGCCGATGCCAAGCATTCCCGGGACAAGACTCAAAAAGTAGATGGCAACATATCACGCCTGCTTTTAGAGTTGATCCAAGTGGTGAGGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

327

Amino Acids

36.15

Weight (kDa)

8.9

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019875)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0105231
rosa_multiflora Rmu_sc0004528.1_g000008
rosa_roxburghii Rroxscaffold_2G00131210 Rroxscaffold_4G00315670 Rroxscaffold_6G00404010
rosa_samantha Rh2DG563800
rosa_wichuraiana Rw2G018290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 631
Acc36I ACCTGC 1 cut(s) 631
AccBSI CCGCTC 2 cut(s) 705, 856
AciI CCGC 7 cut(s) 231, 393, 407, 532, 535, 703, 854
AclI AACGTT 1 cut(s) 288
AclWI GGATC 2 cut(s) 697, 955
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 1 cut(s) 373
AcyI GRCGYC 2 cut(s) 356, 410
AdeI CACNNNGTG 1 cut(s) 403
AfaI GTAC 2 cut(s) 513, 682
AfiI CCNNNNNNNGG 3 cut(s) 290, 406, 580
AgsI TTSAA 5 cut(s) 125, 205, 227, 644, 783
AjnI CCWGG 1 cut(s) 822
AluBI AGCT 3 cut(s) 32, 50, 629
AluI AGCT 3 cut(s) 32, 50, 629
Alw26I GTCTC 2 cut(s) 274, 679
AlwI GGATC 2 cut(s) 697, 955
Ama87I CYCGRG 1 cut(s) 906
AoxI GGCC 2 cut(s) 152, 557
ApeKI GCWGC 4 cut(s) 47, 50, 529, 626
ApoI RAATTY 1 cut(s) 373
Asp700I GAANNNNTTC 1 cut(s) 831
AspLEI GCGC 1 cut(s) 588
AspS9I GGNCC 3 cut(s) 181, 386, 557
AsuC2I CCSGG 4 cut(s) 384, 401, 907, 908
AsuHPI GGTGA 2 cut(s) 83, 982
AsuII TTCGAA 1 cut(s) 835
AsuNHI GCTAGC 1 cut(s) 32
AvaI CYCGRG 1 cut(s) 906
AvaII GGWCC 2 cut(s) 181, 386
BalI TGGCCA 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 355
BbvI GCAGC 4 cut(s) 37, 59, 541, 638
BccI CCATC 4 cut(s) 263, 536, 540, 923
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 1 cut(s) 824
BcnI CCSGG 4 cut(s) 384, 401, 907, 908
BcoDI GTCTC 2 cut(s) 274, 679
BfaI CTAG 5 cut(s) 33, 114, 462, 570, 768
BfmI CTRYAG 2 cut(s) 51, 624
BfuAI ACCTGC 1 cut(s) 631
BisI GCNGC 6 cut(s) 48, 51, 408, 530, 533, 627
BlsI GCNGC 6 cut(s) 49, 52, 409, 531, 534, 628
BmcAI AGTACT 1 cut(s) 513
Bme1390I CCNGG 5 cut(s) 384, 401, 824, 907, 908
Bme18I GGWCC 2 cut(s) 181, 386
BmeT110I CYCGRG 1 cut(s) 906
BmgT120I GGNCC 3 cut(s) 181, 386, 557
BmiI GGNNCC 2 cut(s) 324, 558
BmrFI CCNGG 5 cut(s) 384, 401, 824, 907, 908
BmsI GCATC 3 cut(s) 824, 838, 883
BmtI GCTAGC 1 cut(s) 36
BpiI GAAGAC 1 cut(s) 355
Bpu14I TTCGAA 1 cut(s) 835
BpuMI CCSGG 4 cut(s) 384, 401, 907, 908
BsaHI GRCGYC 2 cut(s) 356, 410
BsaI GGTCTC 1 cut(s) 274
BsaJI CCNNGG 3 cut(s) 498, 822, 906
BsaWI WCCGGW 1 cut(s) 446
Bsc4I CCNNNNNNNGG 3 cut(s) 290, 406, 580
Bse3DI GCAATG 1 cut(s) 859
BseBI CCWGG 1 cut(s) 824
BseDI CCNNGG 3 cut(s) 498, 822, 906
BseGI GGATG 2 cut(s) 551, 815
BseLI CCNNNNNNNGG 3 cut(s) 290, 406, 580
BseMI GCAATG 1 cut(s) 859
BseMII CTCAG 1 cut(s) 623
BseXI GCAGC 4 cut(s) 37, 59, 541, 638
BsgI GTGCAG 2 cut(s) 66, 548
BshFI GGCC 2 cut(s) 154, 559
BsiHKCI CYCGRG 1 cut(s) 906
BsiSI CCGG 4 cut(s) 384, 401, 447, 907
BslFI GGGAC 2 cut(s) 685, 923
BslI CCNNNNNNNGG 3 cut(s) 290, 406, 580
BsmAI GTCTC 2 cut(s) 274, 679
BsmBI CGTCTC 1 cut(s) 679
BsmFI GGGAC 2 cut(s) 685, 923
BsmI GAATGC 2 cut(s) 664, 901
BsnI GGCC 2 cut(s) 154, 559
Bso31I GGTCTC 1 cut(s) 274
BsoBI CYCGRG 1 cut(s) 906
Bsp119I TTCGAA 1 cut(s) 835
Bsp143I GATC 3 cut(s) 658, 689, 960
BspACI CCGC 7 cut(s) 231, 393, 407, 532, 535, 703, 854
BspANI GGCC 2 cut(s) 154, 559
BspCNI CTCAG 1 cut(s) 622
BspLI GGNNCC 2 cut(s) 324, 558
BspMAI CTGCAG 2 cut(s) 55, 628
BspMI ACCTGC 1 cut(s) 631
BspOI GCTAGC 1 cut(s) 36
BspPI GGATC 2 cut(s) 697, 955
BspT104I TTCGAA 1 cut(s) 835
BspTNI GGTCTC 1 cut(s) 274
BsrBI CCGCTC 2 cut(s) 705, 856
BsrDI GCAATG 1 cut(s) 859
BssECI CCNNGG 3 cut(s) 498, 822, 906
BssMI GATC 3 cut(s) 658, 689, 960
BssNI GRCGYC 2 cut(s) 356, 410
BssT1I CCWWGG 1 cut(s) 498
Bst2UI CCWGG 1 cut(s) 824
Bst4CI ACNGT 2 cut(s) 86, 673
Bst6I CTCTTC 3 cut(s) 255, 272, 836
BstACI GRCGYC 2 cut(s) 356, 410
BstBI TTCGAA 1 cut(s) 835
BstC8I GCNNGC 3 cut(s) 34, 189, 947
BstDEI CTNAG 2 cut(s) 609, 774
BstEII GGTNACC 1 cut(s) 71
BstF5I GGATG 2 cut(s) 551, 815
BstHHI GCGC 1 cut(s) 588
BstKTI GATC 3 cut(s) 661, 692, 963
BstMAI GTCTC 2 cut(s) 274, 679
BstMBI GATC 3 cut(s) 658, 689, 960
BstMWI GCNNNNNNNGC 2 cut(s) 413, 565
BstNI CCWGG 1 cut(s) 824
BstPI GGTNACC 1 cut(s) 71
BstSCI CCNGG 5 cut(s) 382, 399, 822, 905, 906
BstSFI CTRYAG 2 cut(s) 51, 624
BstV1I GCAGC 4 cut(s) 37, 59, 541, 638
BstV2I GAAGAC 1 cut(s) 355
BstX2I RGATCY 1 cut(s) 689
BstXI CCANNNNNNTGG 1 cut(s) 65
BstYI RGATCY 1 cut(s) 689
BsuRI GGCC 2 cut(s) 154, 559
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 551, 815
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 3 cut(s) 34, 189, 947
CfoI GCGC 1 cut(s) 588
Cfr13I GGNCC 3 cut(s) 181, 386, 557
Cfr9I CCCGGG 1 cut(s) 906
CseI GACGC 2 cut(s) 345, 399
Csp6I GTAC 2 cut(s) 512, 681
CviAII CATG 7 cut(s) 59, 156, 168, 197, 678, 803, 879
CviJI RGCY 9 cut(s) 32, 50, 110, 154, 428, 559, 573, 629, 890
CviKI_1 RGCY 9 cut(s) 32, 50, 110, 154, 428, 559, 573, 629, 890
CviQI GTAC 2 cut(s) 512, 681
DdeI CTNAG 2 cut(s) 609, 774
DpnI GATC 3 cut(s) 660, 691, 962
DpnII GATC 3 cut(s) 658, 689, 960
DraIII CACNNNGTG 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 152
Eam1104I CTCTTC 3 cut(s) 255, 272, 836
EarI CTCTTC 3 cut(s) 255, 272, 836
Eco130I CCWWGG 1 cut(s) 498
Eco31I GGTCTC 1 cut(s) 274
Eco47I GGWCC 2 cut(s) 181, 386
Eco88I CYCGRG 1 cut(s) 906
Eco91I GGTNACC 1 cut(s) 71
EcoO65I GGTNACC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 822
EcoT14I CCWWGG 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 498
Esp3I CGTCTC 1 cut(s) 679
FaeI CATG 7 cut(s) 62, 159, 171, 200, 681, 806, 882
FaqI GGGAC 2 cut(s) 685, 923
FatI CATG 7 cut(s) 58, 155, 167, 196, 677, 802, 878
FauI CCCGC 3 cut(s) 238, 528, 710
FauNDI CATATG 1 cut(s) 214
Fnu4HI GCNGC 6 cut(s) 48, 51, 408, 530, 533, 627
FokI GGATG 2 cut(s) 558, 802
Fsp4HI GCNGC 6 cut(s) 48, 51, 408, 530, 533, 627
FspBI CTAG 5 cut(s) 33, 114, 462, 570, 768
GlaI GCGC 1 cut(s) 587
GluI GCNGC 6 cut(s) 48, 51, 408, 530, 533, 627
HaeIII GGCC 2 cut(s) 154, 559
HapII CCGG 4 cut(s) 384, 401, 447, 907
HgaI GACGC 2 cut(s) 345, 399
HhaI GCGC 1 cut(s) 588
Hin1I GRCGYC 2 cut(s) 356, 410
Hin1II CATG 7 cut(s) 62, 159, 171, 200, 681, 806, 882
Hin6I GCGC 1 cut(s) 586
HinP1I GCGC 1 cut(s) 586
HinfI GANTC 4 cut(s) 551, 644, 779, 916
HpaII CCGG 4 cut(s) 384, 401, 447, 907
HphI GGTGA 2 cut(s) 83, 982
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 1 cut(s) 658
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 1 cut(s) 89
Hpy99I CGWCG 4 cut(s) 361, 415, 493, 524
HpyAV CCTTC 4 cut(s) 99, 288, 467, 574
HpyCH4III ACNGT 2 cut(s) 86, 673
HpyCH4IV ACGT 2 cut(s) 288, 491
HpyCH4V TGCA 9 cut(s) 10, 47, 53, 171, 529, 626, 695, 815, 851
HpyF10VI GCNNNNNNNGC 2 cut(s) 413, 565
HpyF3I CTNAG 2 cut(s) 609, 774
HpySE526I ACGT 2 cut(s) 288, 491
Hsp92I GRCGYC 2 cut(s) 356, 410
Hsp92II CATG 7 cut(s) 62, 159, 171, 200, 681, 806, 882
HspAI GCGC 1 cut(s) 586
Kzo9I GATC 3 cut(s) 658, 689, 960
LmnI GCTCC 3 cut(s) 583, 710, 861
Lsp1109I GCAGC 4 cut(s) 37, 59, 541, 638
LweI GCATC 3 cut(s) 824, 838, 883
MaeI CTAG 5 cut(s) 33, 114, 462, 570, 768
MaeII ACGT 2 cut(s) 288, 491
MaeIII GTNAC 1 cut(s) 71
MalI GATC 3 cut(s) 660, 691, 962
MbiI CCGCTC 2 cut(s) 705, 856
MboI GATC 3 cut(s) 658, 689, 960
MboII GAAGA 5 cut(s) 217, 242, 289, 355, 853
MfeI CAATTG 1 cut(s) 979
MflI RGATCY 1 cut(s) 689
MlsI TGGCCA 1 cut(s) 154
MluCI AATT 5 cut(s) 192, 302, 373, 431, 979
MluNI TGGCCA 1 cut(s) 154
MlyI GAGTC 2 cut(s) 560, 910
MmeI TCCRAC 2 cut(s) 271, 413
MnlI CCTC 6 cut(s) 62, 172, 251, 592, 878, 966
Mox20I TGGCCA 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 831
MscI TGGCCA 1 cut(s) 154
MseI TTAA 2 cut(s) 516, 789
Msp20I TGGCCA 1 cut(s) 154
MspA1I CMGCKG 2 cut(s) 50, 532
MspI CCGG 4 cut(s) 384, 401, 447, 907
MspR9I CCNGG 5 cut(s) 384, 401, 824, 907, 908
MunI CAATTG 1 cut(s) 979
Mva1269I GAATGC 2 cut(s) 664, 901
MvaI CCWGG 1 cut(s) 824
MwoI GCNNNNNNNGC 2 cut(s) 413, 565
NciI CCSGG 4 cut(s) 384, 401, 907, 908
NdeI CATATG 1 cut(s) 214
NdeII GATC 3 cut(s) 658, 689, 960
NheI GCTAGC 1 cut(s) 32
NlaIII CATG 7 cut(s) 62, 159, 171, 200, 681, 806, 882
NlaIV GGNNCC 2 cut(s) 324, 558
NmuCI GTSAC 1 cut(s) 71
NspV TTCGAA 1 cut(s) 835
PaqCI CACCTGC 1 cut(s) 631
PctI GAATGC 2 cut(s) 664, 901
PdmI GAANNNNTTC 1 cut(s) 831
PfeI GAWTC 2 cut(s) 644, 779
PkrI GCNGC 6 cut(s) 49, 52, 409, 531, 534, 628
PleI GAGTC 2 cut(s) 559, 910
PpsI GAGTC 2 cut(s) 559, 910
Psp1406I AACGTT 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 822
PspEI GGTNACC 1 cut(s) 71
PspGI CCWGG 1 cut(s) 822
PspN4I GGNNCC 2 cut(s) 324, 558
PspPI GGNCC 3 cut(s) 181, 386, 557
PstI CTGCAG 2 cut(s) 55, 628
PsuI RGATCY 1 cut(s) 689
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 2 cut(s) 513, 682
RsaNI GTAC 2 cut(s) 512, 681
SaqAI TTAA 2 cut(s) 516, 789
SatI GCNGC 6 cut(s) 48, 51, 408, 530, 533, 627
Sau3AI GATC 3 cut(s) 658, 689, 960
Sau96I GGNCC 3 cut(s) 181, 386, 557
ScaI AGTACT 1 cut(s) 513
SchI GAGTC 2 cut(s) 560, 910
ScrFI CCNGG 5 cut(s) 384, 401, 824, 907, 908
SfaNI GCATC 3 cut(s) 824, 838, 883
SfcI CTRYAG 2 cut(s) 51, 624
SfuI TTCGAA 1 cut(s) 835
SinI GGWCC 2 cut(s) 181, 386
SmaI CCCGGG 1 cut(s) 908
Sse9I AATT 5 cut(s) 192, 302, 373, 431, 979
SsiI CCGC 7 cut(s) 231, 393, 407, 532, 535, 703, 854
SspI AATATT 1 cut(s) 100
SspMI CTAG 5 cut(s) 33, 114, 462, 570, 768
StyD4I CCNGG 5 cut(s) 382, 399, 822, 905, 906
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 2 cut(s) 86, 673
TaiI ACGT 2 cut(s) 291, 494
TaqI TCGA 2 cut(s) 632, 835
TaqII GACCGA 1 cut(s) 349
TasI AATT 5 cut(s) 192, 302, 373, 431, 979
TatI WGTACW 2 cut(s) 511, 680
TauI GCSGC 2 cut(s) 410, 535
TfiI GAWTC 2 cut(s) 644, 779
Tru1I TTAA 2 cut(s) 516, 789
Tru9I TTAA 2 cut(s) 516, 789
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 4 cut(s) 47, 50, 529, 626
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 3 cut(s) 185, 726, 819
TspGWI ACGGA 1 cut(s) 356
TspMI CCCGGG 1 cut(s) 906
VpaK11BI GGWCC 2 cut(s) 181, 386
XapI RAATTY 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
XmaI CCCGGG 1 cut(s) 906
XmnI GAANNNNTTC 1 cut(s) 831
XspI CTAG 5 cut(s) 33, 114, 462, 570, 768
ZrmI AGTACT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.