Rmu_sc0004644.1_g000011

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004644.1
OG0009783
Physical Location & Seq
Reverse (-)
77924 .. 84342
6419 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 420 bp
atgccgctaaacttagcgggaggtgccatctcggcactgctatgcctcagtctcaccgccactctgctctccgtggttaccgctgaagatccctaccgtttcttcgaatggaatgtcacttacggcgacatctacccgctaggtgttcgccaaaagggaatacttatcaacggccagttccccggcccggatattcactccgtcaccaatgacaatctcatcatcaatgtcttcaacagcttggacgagccttttctcatctcctggcatcatgtgattctcagcacagctacgccgatgaggcatgtgttgttaacacagatagtctcgtcgatttcgaaccggagatccagactctcggaatctccgtccgtcgtctccgacttcgatttctccgtcagatcaggttcgagtctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

139

Amino Acids

15.26

Weight (kDa)

5.68

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 38 - 93 6.7e-14 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 23
AciI CCGC 5 cut(s) 5, 17, 57, 81, 137
AclWI GGATC 2 cut(s) 83, 342
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 187
AgsI TTSAA 1 cut(s) 235
AjnI CCWGG 1 cut(s) 263
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 2 cut(s) 240, 290
AluI AGCT 2 cut(s) 240, 290
Alw26I GTCTC 3 cut(s) 56, 331, 382
AlwI GGATC 2 cut(s) 83, 342
AoxI GGCC 2 cut(s) 172, 184
ArsI GACNNNNNNTTYG 2 cut(s) 99, 131
AspS9I GGNCC 1 cut(s) 185
AsuC2I CCSGG 2 cut(s) 183, 188
AsuHPI GGTGA 2 cut(s) 46, 196
AsuII TTCGAA 2 cut(s) 105, 338
BanI GGYRCC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 223
BccI CCATC 1 cut(s) 35
BceAI ACGGC 2 cut(s) 139, 187
BciT130I CCWGG 1 cut(s) 265
BcnI CCSGG 2 cut(s) 183, 188
BcoDI GTCTC 3 cut(s) 56, 331, 382
BfaI CTAG 1 cut(s) 140
BglI GCCNNNNNGGC 2 cut(s) 32, 301
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 3 cut(s) 183, 188, 265
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 3 cut(s) 183, 188, 265
BmsI GCATC 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 223
Bpu14I TTCGAA 2 cut(s) 105, 338
BpuMI CCSGG 2 cut(s) 183, 188
BsaJI CCNNGG 2 cut(s) 72, 181
BsaWI WCCGGW 1 cut(s) 342
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 265
BseDI CCNNGG 2 cut(s) 72, 181
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMII CTCAG 2 cut(s) 61, 295
BseNI ACTGG 1 cut(s) 175
BshFI GGCC 2 cut(s) 174, 186
BshNI GGYRCC 1 cut(s) 23
BsiSI CCGG 3 cut(s) 183, 188, 343
BslI CCNNNNNNNGG 1 cut(s) 187
BsmAI GTCTC 3 cut(s) 56, 331, 382
BsmBI CGTCTC 1 cut(s) 382
BsnI GGCC 2 cut(s) 174, 186
Bsp119I TTCGAA 2 cut(s) 105, 338
Bsp143I GATC 3 cut(s) 88, 347, 401
BspACI CCGC 5 cut(s) 5, 17, 57, 81, 137
BspANI GGCC 2 cut(s) 174, 186
BspCNI CTCAG 2 cut(s) 60, 294
BspLI GGNNCC 1 cut(s) 25
BspPI GGATC 2 cut(s) 83, 342
BspT104I TTCGAA 2 cut(s) 105, 338
BspT107I GGYRCC 1 cut(s) 23
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 72, 181
BssMI GATC 3 cut(s) 88, 347, 401
Bst2UI CCWGG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 98
BstBI TTCGAA 2 cut(s) 105, 338
BstDEI CTNAG 3 cut(s) 13, 47, 281
BstDSI CCRYGG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 76
BstKTI GATC 3 cut(s) 91, 350, 404
BstMAI GTCTC 3 cut(s) 56, 331, 382
BstMBI GATC 3 cut(s) 88, 347, 401
BstMWI GCNNNNNNNGC 3 cut(s) 23, 32, 301
BstNI CCWGG 1 cut(s) 265
BstNSI RCATGY 1 cut(s) 308
BstPI GGTNACC 1 cut(s) 76
BstSCI CCNGG 3 cut(s) 181, 186, 263
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 2 cut(s) 88, 347
BstYI RGATCY 2 cut(s) 88, 347
BsuRI GGCC 2 cut(s) 174, 186
BtgI CCRYGG 1 cut(s) 72
BtsI GCAGTG 1 cut(s) 35
BtsIMutI CAGTG 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 185
CviAII CATG 2 cut(s) 272, 305
CviJI RGCY 5 cut(s) 174, 186, 240, 250, 290
CviKI_1 RGCY 5 cut(s) 174, 186, 240, 250, 290
DdeI CTNAG 3 cut(s) 13, 47, 281
DpnI GATC 3 cut(s) 90, 349, 403
DpnII GATC 3 cut(s) 88, 347, 401
EaeI YGGCCR 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 105
Eco91I GGTNACC 1 cut(s) 76
EcoO65I GGTNACC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 263
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 2 cut(s) 275, 308
FaiI YATR 3 cut(s) 43, 273, 306
FatI CATG 2 cut(s) 271, 304
FauI CCCGC 2 cut(s) 10, 144
Fnu4HI GCNGC 1 cut(s) 5
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 1 cut(s) 140
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 2 cut(s) 174, 186
HapII CCGG 3 cut(s) 183, 188, 343
Hin1II CATG 2 cut(s) 275, 308
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HinfI GANTC 4 cut(s) 277, 354, 362, 412
HpaI GTTAAC 1 cut(s) 315
HpaII CCGG 3 cut(s) 183, 188, 343
HphI GGTGA 2 cut(s) 46, 196
Hpy166II GTNNAC 1 cut(s) 315
Hpy188I TCNGA 3 cut(s) 361, 382, 401
Hpy188III TCNNGA 1 cut(s) 351
Hpy8I GTNNAC 1 cut(s) 315
Hpy99I CGWCG 2 cut(s) 334, 377
HpyCH4III ACNGT 1 cut(s) 98
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 32, 301
HpyF3I CTNAG 3 cut(s) 13, 47, 281
Hsp92II CATG 2 cut(s) 275, 308
KspAI GTTAAC 1 cut(s) 315
Kzo9I GATC 3 cut(s) 88, 347, 401
LpnPI CCDG 8 cut(s) 188, 196, 201, 250, 277, 356, 364, 390
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 140
MaeIII GTNAC 3 cut(s) 76, 115, 202
MalI GATC 3 cut(s) 90, 349, 403
MboI GATC 3 cut(s) 88, 347, 401
MboII GAAGA 3 cut(s) 94, 98, 223
MflI RGATCY 2 cut(s) 88, 347
MlyI GAGTC 1 cut(s) 348
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 3 cut(s) 14, 56, 294
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 1 cut(s) 40
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 3 cut(s) 183, 188, 343
MspR9I CCNGG 3 cut(s) 183, 188, 265
MvaI CCWGG 1 cut(s) 265
MwoI GCNNNNNNNGC 3 cut(s) 23, 32, 301
NciI CCSGG 2 cut(s) 183, 188
NdeII GATC 3 cut(s) 88, 347, 401
NlaIII CATG 2 cut(s) 275, 308
NlaIV GGNNCC 1 cut(s) 25
NmeAIII GCCGAG 1 cut(s) 11
NmuCI GTSAC 2 cut(s) 115, 202
NspI RCATGY 1 cut(s) 308
NspV TTCGAA 2 cut(s) 105, 338
PcsI WCGNNNNNNNCGW 3 cut(s) 335, 365, 393
PfeI GAWTC 2 cut(s) 277, 362
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 348
PpsI GAGTC 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 263
PspEI GGTNACC 1 cut(s) 76
PspGI CCWGG 1 cut(s) 263
PspN4I GGNNCC 1 cut(s) 25
PspPI GGNCC 1 cut(s) 185
PsuI RGATCY 2 cut(s) 88, 347
RseI CAYNNNNRTG 1 cut(s) 40
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 3 cut(s) 88, 347, 401
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 1 cut(s) 348
ScrFI CCNGG 3 cut(s) 183, 188, 265
SetI ASST 5 cut(s) 25, 145, 242, 292, 409
SfaNI GCATC 1 cut(s) 277
SfuI TTCGAA 2 cut(s) 105, 338
SmiMI CAYNNNNRTG 1 cut(s) 40
SsiI CCGC 5 cut(s) 5, 17, 57, 81, 137
SspMI CTAG 1 cut(s) 140
StyD4I CCNGG 3 cut(s) 181, 186, 263
TaaI ACNGT 1 cut(s) 98
TaqI TCGA 5 cut(s) 105, 332, 338, 387, 410
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 2 cut(s) 277, 362
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 2 cut(s) 115, 202
Tsp45I GTSAC 2 cut(s) 115, 202
TspGWI ACGGA 5 cut(s) 61, 190, 357, 361, 385
TspRI CASTG 1 cut(s) 42
XceI RCATGY 1 cut(s) 308
XspI CTAG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.