Rmu_sc0005298.1_g000007

Ribonuclease T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005298.1
OG0020678
Physical Location & Seq
Forward (+)
36869 .. 39058
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 921 bp
atgatcagagatgcggtcggacagccggtcccccagcctgtcggagagctggtcccatcactcccatggaggttcggacggccgtattatgagtacctgcgatttgtactgcaagttcccagaacattgaatccaagctcaacacttaaccagaatttcaccattcatggcctatggcctagcaatcacagcactcataatggtggaattccttatgttcaaaattgtgatccaaattatatccttgatcctcaacaggaggtgagccgtaacctccaaaggaatctcagtcactcttggcccgatgtaaagccgactgcaaacgattcgaagttttggaccgacgaatacaggaagcacggcacctgcgcaaaccgtatattccgtacaccaggggattactttagaaaagcagatgacttgtatcgcagtcatcccatacaacgtttttttaatctattcgccgaccacatccgcccaggacagcaagtccccgtgagcacatttattaatctcgtaccaacggcgacagcaaggaagaatgcacgactgtggtgcaattggaatggaaccctctacctactgaaggaagtaaataatctcatactcgtccagttctgctactgcacttgtactcgtgttctgctactgcactctcactcttcgatcttagaggtttcggaatctggaactccattacaattccatcaccacatggagccgcgacagaatctttctccgccatcttcttcagaaatcaccgaagtcgcaaggtggtggagatcgaaagacgattctgagtcatcaatgaagttcgaaacctgcaccaatgtttcggatctcggcgagcctagttccggcatgcacgacgtctttctcaatggacttggcattttttgctgctgctcttccctttcttag

Protein Analysis

306

Amino Acids

35.1

Weight (kDa)

7.63

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 47 - 199 1.9e-24 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020678)

Species Orthologous Gene IDs
rosa_laevigata RLG00000025746
rosa_multiflora Rmu_sc0005298.1_g000007 Rmu_sc0010184.1_g000001
rosa_roxburghii Rroxscaffold_6G00427960
rosa_samantha Rh2CG051700 Rh5DG089900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 374
AatII GACGTC 1 cut(s) 873
Acc16I TGCGCA 1 cut(s) 370
Acc36I ACCTGC 3 cut(s) 105, 374, 830
AccB1I GGYRCC 1 cut(s) 362
AccII CGCG 1 cut(s) 724
AciI CCGC 4 cut(s) 14, 475, 722, 740
AclI AACGTT 1 cut(s) 445
AclWI GGATC 3 cut(s) 224, 242, 846
AcoI YGGCCR 1 cut(s) 80
AcsI RAATTY 2 cut(s) 154, 207
AcuI CTGAAG 2 cut(s) 605, 735
AcyI GRCGYC 1 cut(s) 870
AfaI GTAC 5 cut(s) 95, 108, 388, 519, 634
AfiI CCNNNNNNNGG 2 cut(s) 586, 857
AgsI TTSAA 2 cut(s) 130, 221
AhdI GACNNNNNGTC 2 cut(s) 26, 488
AjnI CCWGG 2 cut(s) 391, 478
AleI CACNNNNGTG 1 cut(s) 550
AloI GAACNNNNNNTCC 2 cut(s) 115, 147
AluBI AGCT 2 cut(s) 49, 138
AluI AGCT 2 cut(s) 49, 138
Alw21I GWGCWC 1 cut(s) 503
AlwI GGATC 3 cut(s) 224, 242, 846
AoxI GGCC 4 cut(s) 80, 169, 176, 299
ApeKI GCWGC 2 cut(s) 900, 903
ApoI RAATTY 2 cut(s) 154, 207
AseI ATTAAT 1 cut(s) 510
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 4 cut(s) 28, 52, 300, 339
AsuHPI GGTGA 4 cut(s) 151, 274, 701, 751
AsuII TTCGAA 2 cut(s) 329, 816
AvaII GGWCC 3 cut(s) 28, 52, 339
BanI GGYRCC 1 cut(s) 362
BauI CACGAG 1 cut(s) 636
Bbv12I GWGCWC 1 cut(s) 503
BbvI GCAGC 2 cut(s) 887, 890
BccI CCATC 3 cut(s) 64, 714, 751
BceAI ACGGC 5 cut(s) 67, 95, 252, 376, 540
BcgI CGANNNNNNTGC 4 cut(s) 309, 343, 537, 571
BciT130I CCWGG 2 cut(s) 393, 480
BclI TGATCA 1 cut(s) 3
BfaI CTAG 2 cut(s) 180, 852
BfuAI ACCTGC 3 cut(s) 105, 374, 830
BisI GCNGC 3 cut(s) 722, 901, 904
BlsI GCNGC 3 cut(s) 723, 902, 905
Bme1390I CCNGG 2 cut(s) 393, 480
Bme18I GGWCC 3 cut(s) 28, 52, 339
BmeRI GACNNNNNGTC 2 cut(s) 26, 488
BmgT120I GGNCC 4 cut(s) 28, 52, 300, 339
BmiI GGNNCC 5 cut(s) 30, 54, 364, 571, 720
BmrFI CCNGG 2 cut(s) 393, 480
Bpu14I TTCGAA 2 cut(s) 329, 816
BsaHI GRCGYC 1 cut(s) 870
BsaJI CCNNGG 3 cut(s) 65, 392, 478
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 2 cut(s) 586, 857
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 613
BseBI CCWGG 2 cut(s) 393, 480
BseDI CCNNGG 3 cut(s) 65, 392, 478
BseGI GGATG 2 cut(s) 433, 471
BseLI CCNNNNNNNGG 2 cut(s) 586, 857
BseMII CTCAG 2 cut(s) 301, 789
BseNI ACTGG 1 cut(s) 613
BseX3I CGGCCG 1 cut(s) 80
BseXI GCAGC 2 cut(s) 887, 890
BseYI CCCAGC 1 cut(s) 33
BsgI GTGCAG 3 cut(s) 610, 635, 808
Bsh1236I CGCG 1 cut(s) 724
Bsh1285I CGRYCG 2 cut(s) 18, 83
BshFI GGCC 4 cut(s) 82, 171, 178, 301
BshNI GGYRCC 1 cut(s) 362
BsiEI CGRYCG 2 cut(s) 18, 83
BsiHKAI GWGCWC 1 cut(s) 503
BsiSI CCGG 2 cut(s) 26, 858
BslFI GGGAC 3 cut(s) 14, 38, 476
BslI CCNNNNNNNGG 2 cut(s) 586, 857
BsmFI GGGAC 3 cut(s) 14, 38, 476
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 4 cut(s) 82, 171, 178, 301
Bsp119I TTCGAA 2 cut(s) 329, 816
Bsp1286I GDGCHC 1 cut(s) 503
Bsp143I GATC 6 cut(s) 3, 229, 247, 666, 782, 838
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 4 cut(s) 14, 475, 722, 740
BspANI GGCC 4 cut(s) 82, 171, 178, 301
BspCNI CTCAG 2 cut(s) 300, 790
BspFNI CGCG 1 cut(s) 724
BspLI GGNNCC 5 cut(s) 30, 54, 364, 571, 720
BspMI ACCTGC 3 cut(s) 105, 374, 830
BspPI GGATC 3 cut(s) 224, 242, 846
BspQI GCTCTTC 1 cut(s) 913
BspT104I TTCGAA 2 cut(s) 329, 816
BspT107I GGYRCC 1 cut(s) 362
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 613
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 3 cut(s) 65, 392, 478
BssMI GATC 6 cut(s) 3, 229, 247, 666, 782, 838
BssNI GRCGYC 1 cut(s) 870
BssSI CACGAG 1 cut(s) 636
BssT1I CCWWGG 1 cut(s) 65
Bst2BI CACGAG 1 cut(s) 636
Bst2UI CCWGG 2 cut(s) 393, 480
Bst4CI ACNGT 2 cut(s) 377, 552
Bst6I CTCTTC 2 cut(s) 667, 913
BstACI GRCGYC 1 cut(s) 870
BstAPI GCANNNNNTGC 1 cut(s) 897
BstBI TTCGAA 2 cut(s) 329, 816
BstC8I GCNNGC 2 cut(s) 848, 863
BstDEI CTNAG 4 cut(s) 287, 670, 798, 918
BstDSI CCRYGG 1 cut(s) 65
BstENI CCTNNNNNAGG 1 cut(s) 584
BstF5I GGATG 2 cut(s) 433, 471
BstFNI CGCG 1 cut(s) 724
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 6 cut(s) 6, 232, 250, 669, 785, 841
BstMBI GATC 6 cut(s) 3, 229, 247, 666, 782, 838
BstMCI CGRYCG 2 cut(s) 18, 83
BstMWI GCNNNNNNNGC 2 cut(s) 189, 897
BstNI CCWGG 2 cut(s) 393, 480
BstNSI RCATGY 1 cut(s) 865
BstSCI CCNGG 2 cut(s) 391, 478
BstUI CGCG 1 cut(s) 724
BstV1I GCAGC 2 cut(s) 887, 890
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
BstZI CGGCCG 1 cut(s) 80
BsuRI GGCC 4 cut(s) 82, 171, 178, 301
BtgI CCRYGG 1 cut(s) 65
BtsCI GGATG 2 cut(s) 433, 471
BveI ACCTGC 3 cut(s) 105, 374, 830
Cac8I GCNNGC 2 cut(s) 848, 863
CfoI GCGC 1 cut(s) 371
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 4 cut(s) 28, 52, 300, 339
Csp6I GTAC 5 cut(s) 94, 107, 387, 518, 633
CviAII CATG 4 cut(s) 66, 167, 715, 862
CviQI GTAC 5 cut(s) 94, 107, 387, 518, 633
DdeI CTNAG 4 cut(s) 287, 670, 798, 918
DpnI GATC 6 cut(s) 5, 231, 249, 668, 784, 840
DpnII GATC 6 cut(s) 3, 229, 247, 666, 782, 838
DriI GACNNNNNGTC 2 cut(s) 26, 488
EaeI YGGCCR 1 cut(s) 80
EagI CGGCCG 1 cut(s) 80
Eam1104I CTCTTC 2 cut(s) 667, 913
Eam1105I GACNNNNNGTC 2 cut(s) 26, 488
EarI CTCTTC 2 cut(s) 667, 913
EciI GGCGGA 2 cut(s) 464, 729
EclXI CGGCCG 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 65
Eco47I GGWCC 3 cut(s) 28, 52, 339
Eco52I CGGCCG 1 cut(s) 80
Eco57I CTGAAG 2 cut(s) 605, 735
EcoNI CCTNNNNNAGG 1 cut(s) 584
EcoRI GAATTC 1 cut(s) 207
EcoRII CCWGG 2 cut(s) 391, 478
EcoT14I CCWWGG 1 cut(s) 65
ErhI CCWWGG 1 cut(s) 65
FaeI CATG 4 cut(s) 69, 170, 718, 865
FaqI GGGAC 3 cut(s) 14, 38, 476
FatI CATG 4 cut(s) 65, 166, 714, 861
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 722, 901, 904
FokI GGATG 2 cut(s) 420, 458
Fsp4HI GCNGC 3 cut(s) 722, 901, 904
FspBI CTAG 2 cut(s) 180, 852
FspI TGCGCA 1 cut(s) 370
GlaI GCGC 1 cut(s) 370
GluI GCNGC 3 cut(s) 722, 901, 904
GsaI CCCAGC 1 cut(s) 37
HaeIII GGCC 4 cut(s) 82, 171, 178, 301
HapII CCGG 2 cut(s) 26, 858
HhaI GCGC 1 cut(s) 371
Hin1I GRCGYC 1 cut(s) 870
Hin1II CATG 4 cut(s) 69, 170, 718, 865
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HinfI GANTC 7 cut(s) 130, 283, 326, 683, 730, 794, 800
HpaII CCGG 2 cut(s) 26, 858
HphI GGTGA 4 cut(s) 151, 274, 701, 751
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 8 cut(s) 8, 20, 44, 77, 682, 754, 799, 838
Hpy188III TCNNGA 1 cut(s) 687
Hpy8I GTNNAC 1 cut(s) 389
Hpy99I CGWCG 2 cut(s) 347, 872
HpyAV CCTTC 1 cut(s) 580
HpyCH4III ACNGT 2 cut(s) 377, 552
HpyCH4IV ACGT 2 cut(s) 445, 870
HpyCH4V TGCA 8 cut(s) 112, 320, 545, 558, 627, 652, 825, 865
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 897
HpyF3I CTNAG 4 cut(s) 287, 670, 798, 918
HpySE526I ACGT 2 cut(s) 445, 870
Hsp92I GRCGYC 1 cut(s) 870
Hsp92II CATG 4 cut(s) 69, 170, 718, 865
HspAI GCGC 1 cut(s) 369
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 229, 247, 666, 782, 838
LguI GCTCTTC 1 cut(s) 913
LmnI GCTCC 1 cut(s) 718
Lsp1109I GCAGC 2 cut(s) 887, 890
MaeI CTAG 2 cut(s) 180, 852
MaeII ACGT 2 cut(s) 445, 870
MaeIII GTNAC 2 cut(s) 269, 290
MalI GATC 6 cut(s) 5, 231, 249, 668, 784, 840
MboI GATC 6 cut(s) 3, 229, 247, 666, 782, 838
MboII GAAGA 5 cut(s) 550, 654, 738, 741, 900
MfeI CAATTG 1 cut(s) 559
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 1 cut(s) 503
MluCI AATT 6 cut(s) 154, 207, 223, 235, 559, 701
MlyI GAGTC 1 cut(s) 809
MmeI TCCRAC 1 cut(s) 22
MnlI CCTC 6 cut(s) 63, 253, 261, 284, 584, 667
MseI TTAA 3 cut(s) 147, 453, 510
MslI CAYNNNNRTG 3 cut(s) 64, 201, 550
MspI CCGG 2 cut(s) 26, 858
MspR9I CCNGG 2 cut(s) 393, 480
MunI CAATTG 1 cut(s) 559
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 2 cut(s) 393, 480
MvnI CGCG 1 cut(s) 724
MwoI GCNNNNNNNGC 2 cut(s) 189, 897
NcoI CCATGG 1 cut(s) 65
NdeII GATC 6 cut(s) 3, 229, 247, 666, 782, 838
NlaIII CATG 4 cut(s) 69, 170, 718, 865
NlaIV GGNNCC 5 cut(s) 30, 54, 364, 571, 720
NmeAIII GCCGAG 1 cut(s) 822
NmuCI GTSAC 1 cut(s) 290
NsbI TGCGCA 1 cut(s) 370
NspI RCATGY 1 cut(s) 865
NspV TTCGAA 2 cut(s) 329, 816
OliI CACNNNNGTG 1 cut(s) 550
PaeI GCATGC 1 cut(s) 865
PaqCI CACCTGC 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 913
PctI GAATGC 1 cut(s) 547
PfeI GAWTC 6 cut(s) 130, 283, 326, 683, 730, 794
PkrI GCNGC 3 cut(s) 723, 902, 905
PleI GAGTC 1 cut(s) 808
PpsI GAGTC 1 cut(s) 808
PshBI ATTAAT 1 cut(s) 510
Psp1406I AACGTT 1 cut(s) 445
Psp6I CCWGG 2 cut(s) 391, 478
PspFI CCCAGC 1 cut(s) 33
PspGI CCWGG 2 cut(s) 391, 478
PspN4I GGNNCC 5 cut(s) 30, 54, 364, 571, 720
PspPI GGNCC 4 cut(s) 28, 52, 300, 339
PsrI GAACNNNNNNTAC 2 cut(s) 99, 131
PsuI RGATCY 1 cut(s) 838
RsaI GTAC 5 cut(s) 95, 108, 388, 519, 634
RsaNI GTAC 5 cut(s) 94, 107, 387, 518, 633
RseI CAYNNNNRTG 3 cut(s) 64, 201, 550
SapI GCTCTTC 1 cut(s) 913
SaqAI TTAA 3 cut(s) 147, 453, 510
SatI GCNGC 3 cut(s) 722, 901, 904
Sau3AI GATC 6 cut(s) 3, 229, 247, 666, 782, 838
Sau96I GGNCC 4 cut(s) 28, 52, 300, 339
SchI GAGTC 1 cut(s) 809
ScrFI CCNGG 2 cut(s) 393, 480
SduI GDGCHC 1 cut(s) 503
SfuI TTCGAA 2 cut(s) 329, 816
SinI GGWCC 3 cut(s) 28, 52, 339
SmiMI CAYNNNNRTG 3 cut(s) 64, 201, 550
SphI GCATGC 1 cut(s) 865
Sse9I AATT 6 cut(s) 154, 207, 223, 235, 559, 701
SsiI CCGC 4 cut(s) 14, 475, 722, 740
SspMI CTAG 2 cut(s) 180, 852
StyD4I CCNGG 2 cut(s) 391, 478
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 2 cut(s) 377, 552
TaiI ACGT 2 cut(s) 448, 873
TaqI TCGA 4 cut(s) 329, 665, 785, 816
TaqII GACCGA 1 cut(s) 356
TasI AATT 6 cut(s) 154, 207, 223, 235, 559, 701
TatI WGTACW 2 cut(s) 106, 632
TauI GCSGC 1 cut(s) 724
TfiI GAWTC 6 cut(s) 130, 283, 326, 683, 730, 794
Tru1I TTAA 3 cut(s) 147, 453, 510
Tru9I TTAA 3 cut(s) 147, 453, 510
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 2 cut(s) 900, 903
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 2 cut(s) 155, 824
TspGWI ACGGA 1 cut(s) 374
VpaK11BI GGWCC 3 cut(s) 28, 52, 339
VspI ATTAAT 1 cut(s) 510
XagI CCTNNNNNAGG 1 cut(s) 584
XapI RAATTY 2 cut(s) 154, 207
XceI RCATGY 1 cut(s) 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XspI CTAG 2 cut(s) 180, 852
ZraI GACGTC 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.