Rmu_sc0005366.1_g000012

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005366.1
OG0015155
Physical Location & Seq
Forward (+)
59257 .. 66500
7244 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4176 bp
atggtgaggacgaagaaaggaggacgaccggccactctgatttcagaagaggcaaggatccgaatacgtgacatactccagcagttcctggagtccaatgaagaagtttacacatttgggacagacctgacggttaatgaacgggcttacgtccacgaacagtgccgcaagatggatatgaaatccaagagctccgggtcgggtaaaacgcgcaaggtttcggtttacaggctttttaggatggaggcaatcagcaatggtaaggaaagaatccctcaggtgacgttttccggggacacaagagaagttttgggggatttgtttacgtggtacccacccggagatggagaggaacccaaagaaaggatccgtgaagatagtgacaagtttcagaaactccaccgcaagaaagacccgctgttctgcagaccttcgatgagccagaacgaaattgcaaagaaagtggaaatgcaggctgccaaaatgagaaaagccaagattactaaagagaggtctaacctacccatggcaccctacagggatgtggtaacgtcagcagttgaatctcaccaggttgttcttgtatctggtgagactgggtgcggaaagaccacacaggtgccacaatatcttttggaccatatgtggggtaagggtgaggcatgtaaaatagtgtgcactcaacccaggcgtatctcagccatatcagttgctgagagaatctcttatgaaagaggacagaatgttggggaggatattgggtacaagatacggttggaaagcaaaggtgggaggcactcatctgtggtgttctgcacaaatgggattctactgaggattctaatttccagaggcggtggtaggtcaaaaacagatgcatcgaataaagaaaccaaacaacaagatctctctgacataactcacattattgtggatgaaattcatgaaagggatcgccactctgatttcattctggctattctcagagacatgcttccttcacatcctcatctgcatctgatattgatgagtgcgactgttgatgtggaacggttttcacaatattttggtggctgcccaattatccgtgtcccagggttcacttatcctgtcaaatctttctatttggaggatgtattgtctatcttgagatctgtaagaccgggtagagtaggagatgtttgcatgctgctatcatttggtgcagactgccaattacgggccaaggatggaaccactgcattagagttggctgagcaggtaaaccaattagaatctgctcagatgttgaaagagcatatggaaagtgctcgttctaactctatggaagaacaaaatttgctcaataaatatctccaatattttaatcctgagaatattgatttcattcttatcgagcaattgttagggaaaatttttagtgattcgaaagatggagctattcttgtttttcttccagggtgggatgatataaataaaatgagggacagattactcacgaacccatatttcagagatccttctaagtgtgtgattatatctctacattcaatggttccagctgctgaacagaagaaggtcttcattcgtccacctagtggttgccgaaaaattgttctttcaaccaatattgctgaaactgcagtcactattgatgatgttgtttatgttataaacacgggtcggatgaaagaaaaaaattatgatccttttagtaatgtttcaactcttcaatcttcttgggtgtcaaaagcgaatgccaagcagcgacaggggcgtgcgggccgttgtcagcctggaatctgttatcatctttactctaaacttcgagcagctgcattgtcagattttcaggtgcctgagattaggagaatgccaatcgaggaactttgccttcaggtgaagttgcttgatccagattgcaagatagacgagttcctaaacaagacgctggatcctcctcattctgagaccatacacaatgcgatttctgttcttcgagatatcggggctttgtcagtggacgataaacttacagaacttggggaaaagctaggttctttacctgttcatccattgacaagcaagatgctattctttgccacactgatgaactgtctcgacccagctttaactctggcctgtgctgccgactacaaggatccattctctcttcctatgatgcctgaggaaaaggagaaagctgctgctgctaaggctgaactgtgttcattgtatggtgggaacagtgatcagctagcagtcatagcagcatttgactgctggaagattgcaaaagaacagggtaaagagaatcggttctgtgctaaatactttgtctctgcaagtaccatgcagatgctatctggtatgcgaaagcaactccaaacagagctaattcggctggggtttattcctgaggatgtgtctagatgtagcttgaatgcacgcaatccaggtataatccacgctgtccttgtggctggtctgtatcccatgttgggaagattgctttcttctactaagagtgggaaaaaaaaaggatcctttgtagaaattcctgatggtagtaaagttcgtttgcaccctcgatctgctaattttaaaatagcgttcaaggcacgtggtggctctcctttggttgtatatgatgagataacccgtggggatgggggttcgaatatacgaaactgtactgttgtcgggccacttccattactattgctgtcgacagaaatagctgttgctccagccaatgatgatgagggaagtgatgtcgatgataatgagtgtgaaagtgatgaagatgcaatgaagatagattctaagtcgggtgaacaggccgaagagaagattatgtcatcacctgataaatctgtcatggttatagtggatgggtggctttgttttgagttgaaagctcttgacgttgctcagatctattgcttgagggaacgcctgtctgcagcaatactatttacggttactcatccaaaggaagttttacctcctgatcttggggcatctatttatgctatagcctgtgttctatcttatgatggtctatctgggattccattgccaaagcctatggatttgccgaattcagatttagaacttgagatcggtaaagatggaaaggcacagtctaaagtgacaggaaataaagcagcaaaatcaaaggagccattacgtcagagagtagtttgtcagaggtctctatcgcgagctactgatttacctgccttgaatggatttgcaaagttgtgtgacgacccatcaattcagagcgttcccccaccggctttcgcgaacgctactgatttacctgccttaataaatggagttgcaaagttgagtggcgaccgatcaattcacagtgctcccccaccagcttttgcgaacacaatcccgccgcccacttcacaagttccagcagccaatgatgatgagggaagtgatgtcgatgataatgagtatgaaagtgatgaagatgcaatgaagatagattctaagtcgggtgaacaggccgaagagaagattatgtcatcacctgataaatcagtcatggttatagtggatgggtggctttgttttgagttgaaagctcttgacgttgctcagatctattgcttgagggaacgcctgtctgcagcaatactatttacggttactcatccaaaggaagttttacctcctgatcttggggcatctatttatgctatagcctgtgttctatcttatgatggtctatctgggattccattgccaaagcctatggatttgccgaattcagatttagaacttgagatcggtaaagatggaaaggcacagtttaaagtgacaggaaataaagcagcaaaatcaaaggagccattacgtcagagagtagtttgtcagaggtctctatcgcgagctactgatttacctgccttgaatggatttgcaaagttgtgtgacgacccatcaattcagagcgttcccccaccggctttcgcgaacgctactgatttacctgccttaataaatggagttgcaaagttgagtggcgaccgatcaattcacagtgctcccccaccagcttttgcgaacacaatcccgccgcccacttcacaagttccagcaggtcctgtcgcaggaaacaagcggaagaagcgccggaatcaggctggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007292 GO:0007346 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0009994 GO:0010033 GO:0010467 GO:0012505 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019899 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0032392 GO:0032501 GO:0032502 GO:0032504 GO:0032508 GO:0032991 GO:0034097 GO:0034458 GO:0034612 GO:0034641 GO:0035770 GO:0035821 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043900 GO:0043902 GO:0043903 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044788 GO:0044794 GO:0044827 GO:0044829 GO:0045069 GO:0045070 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0048232 GO:0048468 GO:0048477 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048599 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050792 GO:0050794 GO:0050896 GO:0051276 GO:0051445 GO:0051446 GO:0051702 GO:0051704 GO:0051726 GO:0051728 GO:0051729 GO:0051817 GO:0051851 GO:0060184 GO:0065007 GO:0070035 GO:0070063 GO:0070555 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:0140098 GO:1901360 GO:1901363 GO:1903900 GO:1903902 GO:1990247 GO:1990904 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind R3H

Protein Analysis

1391

Amino Acids

153.06

Weight (kDa)

7.06

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
R3H PF01424 20 - 77 2.6e-08 R3H domain
DEAD PF00270 186 - 348 5.1e-11 DEAD/DEAH box helicase
Helicase_C PF00271 468 - 597 8.7e-11 Helicase conserved C-terminal domain
HA2_C PF21010 692 - 753 1.9e-08 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 822 - 912 8.6e-11 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30800 AT2G30800
fragaria_vesca FvH4_6g38870
malus_domestica MD09G1146500.v1.1
prunus_persica Prupe.3G179000_v2.0.a1
pyrus_communis pycom09g06680
rosa_chinensis RchiOBHm_Chr2g0152461
rosa_laevigata RLG00000020635
rosa_multiflora Rmu_sc0005366.1_g000012
rosa_roxburghii Rroxscaffold_2G00095700
rosa_rugosa Rorug02G0432900
rosa_samantha Rh2AG494700 Rh2BG506900 Rh2CG480900 Rh2DG518100
rosa_wichuraiana Rw2G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1673
Acc36I ACCTGC 6 cut(s) 1249, 3250, 3337, 3937, 4024, 4115
Acc65I GGTACC 1 cut(s) 330
AccB1I GGYRCC 4 cut(s) 330, 529, 619, 1855
AccB7I CCANNNNNTGG 1 cut(s) 1598
AccI GTMKAC 1 cut(s) 2729
AccII CGCG 5 cut(s) 211, 3226, 3311, 3913, 3998
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 6 cut(s) 939, 1336, 1413, 2556, 3103, 3790
AcuI CTGAAG 1 cut(s) 1880
AcvI CACGTG 1 cut(s) 2624
AdeI CACNNNGTG 2 cut(s) 1598, 2627
AfaI GTAC 4 cut(s) 332, 764, 2350, 2695
AjnI CCWGG 7 cut(s) 87, 570, 686, 1093, 1456, 1795, 2455
AleI CACNNNNGTG 1 cut(s) 617
Alw21I GWGCWC 5 cut(s) 194, 680, 1312, 3385, 4072
Alw26I GTCTC 7 cut(s) 587, 981, 1964, 2123, 2344, 3222, 3909
Alw44I GTGCAC 1 cut(s) 676
AlwNI CAGNNNCTG 4 cut(s) 88, 713, 1565, 4130
AoxI GGCC 7 cut(s) 30, 1221, 1783, 2139, 2705, 2841, 3528
ApaLI GTGCAC 1 cut(s) 676
ApoI RAATTY 6 cut(s) 939, 1336, 1413, 2556, 3103, 3790
Asp700I GAANNNNTTC 2 cut(s) 1580, 2318
Asp718I GGTACC 1 cut(s) 330
AspLEI GCGC 2 cut(s) 213, 4158
AspS9I GGNCC 5 cut(s) 637, 1221, 1783, 2705, 4127
AsuC2I CCSGG 4 cut(s) 196, 292, 339, 1164
AsuII TTCGAA 2 cut(s) 1427, 2678
AsuNHI GCTAGC 1 cut(s) 2257
AvaII GGWCC 2 cut(s) 637, 4127
AxyI CCTNAGG 3 cut(s) 276, 2187, 2418
BaeGI GKGCMC 1 cut(s) 680
BamHI GGATCC 5 cut(s) 57, 366, 1954, 2161, 2543
BanI GGYRCC 4 cut(s) 330, 529, 619, 1855
BanII GRGCYC 1 cut(s) 194
BbrPI CACGTG 1 cut(s) 2624
BbsI GAAGAC 1 cut(s) 1573
Bbv12I GWGCWC 5 cut(s) 194, 680, 1312, 3385, 4072
BceAI ACGGC 1 cut(s) 1770
BciT130I CCWGG 7 cut(s) 89, 572, 688, 1095, 1458, 1797, 2457
BciVI GTATCC 1 cut(s) 2502
BclI TGATCA 1 cut(s) 2251
BcnI CCSGG 4 cut(s) 196, 292, 339, 1164
BcoDI GTCTC 7 cut(s) 587, 981, 1964, 2123, 2344, 3222, 3909
BfaI CTAG 4 cut(s) 1596, 2054, 2258, 2430
BfmI CTRYAG 7 cut(s) 424, 535, 1641, 2964, 3036, 3651, 3723
BfoI RGCGCY 1 cut(s) 4159
BfuAI ACCTGC 6 cut(s) 1249, 3250, 3337, 3937, 4024, 4115
BfuI GTATCC 1 cut(s) 2502
BglII AGATCT 4 cut(s) 904, 1151, 2937, 3624
BlpI GCTNAGC 1 cut(s) 1254
Bme18I GGWCC 2 cut(s) 637, 4127
BmgT120I GGNCC 5 cut(s) 637, 1221, 1783, 2705, 4127
BmrI ACTGGG 1 cut(s) 606
BmtI GCTAGC 1 cut(s) 2261
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 1573
BplI GAGNNNNNCTC 2 cut(s) 707, 739
BpmI CTGGAG 3 cut(s) 62, 110, 2733
Bpu10I CCTNAGC 1 cut(s) 2214
Bpu1102I GCTNAGC 1 cut(s) 1254
Bpu14I TTCGAA 2 cut(s) 1427, 2678
BpuEI CTTGAG 5 cut(s) 1168, 2968, 3140, 3655, 3827
BpuMI CCSGG 4 cut(s) 196, 292, 339, 1164
BsaAI YACGTR 3 cut(s) 68, 327, 2624
BsaI GGTCTC 3 cut(s) 1964, 3222, 3909
BsaJI CCNNGG 8 cut(s) 291, 525, 686, 1093, 1094, 1224, 1457, 2662
Bse118I RCCGGY 3 cut(s) 28, 3301, 3988
Bse1I ACTGG 1 cut(s) 601
Bse21I CCTNAGG 3 cut(s) 276, 2187, 2418
Bse3DI GCAATG 5 cut(s) 262, 2817, 3077, 3504, 3764
BseBI CCWGG 7 cut(s) 89, 572, 688, 1095, 1458, 1797, 2457
BseDI CCNNGG 8 cut(s) 291, 525, 686, 1093, 1094, 1224, 1457, 2662
BseMI GCAATG 5 cut(s) 262, 2817, 3077, 3504, 3764
BseNI ACTGG 1 cut(s) 601
BseRI GAGGAG 1 cut(s) 1950
BseSI GKGCMC 1 cut(s) 680
BseYI CCCAGC 2 cut(s) 2125, 2404
BsgI GTGCAG 2 cut(s) 799, 1224
Bsh1236I CGCG 5 cut(s) 211, 3226, 3311, 3913, 3998
Bsh1285I CGRYCG 3 cut(s) 29, 3367, 4054
BshFI GGCC 7 cut(s) 32, 1223, 1785, 2141, 2707, 2843, 3530
BshNI GGYRCC 4 cut(s) 330, 529, 619, 1855
BsiEI CGRYCG 3 cut(s) 29, 3367, 4054
BsiHKAI GWGCWC 5 cut(s) 194, 680, 1312, 3385, 4072
BsiSI CCGG 8 cut(s) 29, 195, 291, 339, 1163, 3302, 3989, 4159
BslFI GGGAC 4 cut(s) 133, 308, 1076, 1499
BsmAI GTCTC 7 cut(s) 587, 981, 1964, 2123, 2344, 3222, 3909
BsmFI GGGAC 4 cut(s) 133, 308, 1076, 1499
BsmI GAATGC 3 cut(s) 1762, 1878, 2449
BsnI GGCC 7 cut(s) 32, 1223, 1785, 2141, 2707, 2843, 3530
Bso31I GGTCTC 3 cut(s) 1964, 3222, 3909
Bsp119I TTCGAA 2 cut(s) 1427, 2678
Bsp1286I GDGCHC 5 cut(s) 194, 680, 1312, 3385, 4072
Bsp1720I GCTNAGC 1 cut(s) 1254
Bsp19I CCATGG 1 cut(s) 525
Bsp68I TCGCGA 4 cut(s) 3226, 3311, 3913, 3998
BspANI GGCC 7 cut(s) 32, 1223, 1785, 2141, 2707, 2843, 3530
BspFNI CGCG 5 cut(s) 211, 3226, 3311, 3913, 3998
BspHI TCATGA 1 cut(s) 943
BspMAI CTGCAG 4 cut(s) 428, 1645, 2968, 3655
BspMI ACCTGC 6 cut(s) 1249, 3250, 3337, 3937, 4024, 4115
BspOI GCTAGC 1 cut(s) 2261
BspT104I TTCGAA 2 cut(s) 1427, 2678
BspT107I GGYRCC 4 cut(s) 330, 529, 619, 1855
BspTNI GGTCTC 3 cut(s) 1964, 3222, 3909
BsrDI GCAATG 5 cut(s) 262, 2817, 3077, 3504, 3764
BsrFI RCCGGY 3 cut(s) 28, 3301, 3988
BsrI ACTGG 1 cut(s) 601
BssAI RCCGGY 3 cut(s) 28, 3301, 3988
BssECI CCNNGG 8 cut(s) 291, 525, 686, 1093, 1094, 1224, 1457, 2662
BssT1I CCWWGG 2 cut(s) 525, 1224
Bst2UI CCWGG 7 cut(s) 89, 572, 688, 1095, 1458, 1797, 2457
Bst6I CTCTTC 5 cut(s) 42, 1734, 2178, 2841, 3528
BstBAI YACGTR 3 cut(s) 68, 327, 2624
BstBI TTCGAA 2 cut(s) 1427, 2678
BstC8I GCNNGC 8 cut(s) 474, 1187, 1779, 1783, 2259, 2449, 3228, 3915
BstDSI CCRYGG 2 cut(s) 525, 2662
BstENI CCTNNNNNAGG 1 cut(s) 4134
BstFNI CGCG 5 cut(s) 211, 3226, 3311, 3913, 3998
BstH2I RGCGCY 1 cut(s) 4159
BstHHI GCGC 2 cut(s) 213, 4158
BstMAI GTCTC 7 cut(s) 587, 981, 1964, 2123, 2344, 3222, 3909
BstMCI CGRYCG 3 cut(s) 29, 3367, 4054
BstMWI GCNNNNNNNGC 9 cut(s) 1640, 1774, 2147, 2210, 2216, 2267, 2401, 2618, 4153
BstNI CCWGG 7 cut(s) 89, 572, 688, 1095, 1458, 1797, 2457
BstNSI RCATGY 3 cut(s) 666, 994, 1189
BstSFI CTRYAG 7 cut(s) 424, 535, 1641, 2964, 3036, 3651, 3723
BstSLI GKGCMC 1 cut(s) 680
BstUI CGCG 5 cut(s) 211, 3226, 3311, 3913, 3998
BstV2I GAAGAC 1 cut(s) 1573
Bsu36I CCTNAGG 3 cut(s) 276, 2187, 2418
BsuI GTATCC 1 cut(s) 2502
BsuRI GGCC 7 cut(s) 32, 1223, 1785, 2141, 2707, 2843, 3530
BtgI CCRYGG 2 cut(s) 525, 2662
BtsI GCAGTG 1 cut(s) 1236
BtsIMutI CAGTG 7 cut(s) 167, 1236, 2025, 2105, 2254, 3385, 4072
BtuMI TCGCGA 4 cut(s) 3226, 3311, 3913, 3998
BveI ACCTGC 6 cut(s) 1249, 3250, 3337, 3937, 4024, 4115
Cac8I GCNNGC 8 cut(s) 474, 1187, 1779, 1783, 2259, 2449, 3228, 3915
CaiI CAGNNNCTG 4 cut(s) 88, 713, 1565, 4130
CciI TCATGA 1 cut(s) 943
CfoI GCGC 2 cut(s) 213, 4158
Cfr10I RCCGGY 3 cut(s) 28, 3301, 3988
Cfr13I GGNCC 5 cut(s) 637, 1221, 1783, 2705, 4127
CseI GACGC 1 cut(s) 1957
CsiI ACCWGGT 1 cut(s) 570
Csp6I GTAC 4 cut(s) 331, 763, 2349, 2694
CspCI CAANNNNNGTGG 4 cut(s) 444, 479, 2605, 2640
CviAII CATG 9 cut(s) 526, 663, 944, 991, 1186, 2353, 2497, 2881, 3568
CviQI GTAC 4 cut(s) 331, 763, 2349, 2694
DraI TTTAAA 2 cut(s) 2605, 3838
DraIII CACNNNGTG 2 cut(s) 1598, 2627
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 5 cut(s) 42, 1734, 2178, 2841, 3528
EarI CTCTTC 5 cut(s) 42, 1734, 2178, 2841, 3528
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 525, 1224
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 3 cut(s) 1964, 3222, 3909
Eco32I GATATC 1 cut(s) 2005
Eco47I GGWCC 2 cut(s) 637, 4127
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 1880
Eco72I CACGTG 1 cut(s) 2624
Eco81I CCTNAGG 3 cut(s) 276, 2187, 2418
EcoICRI GAGCTC 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 4134
EcoO109I RGGNCCY 1 cut(s) 4127
EcoRI GAATTC 2 cut(s) 3103, 3790
EcoRII CCWGG 7 cut(s) 87, 570, 686, 1093, 1456, 1795, 2455
EcoRV GATATC 1 cut(s) 2005
EcoT14I CCWWGG 2 cut(s) 525, 1224
EcoT22I ATGCAT 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 2 cut(s) 525, 1224
FaeI CATG 9 cut(s) 529, 666, 947, 994, 1189, 2356, 2500, 2884, 3571
FalI AAGNNNNNCTT 2 cut(s) 1565, 1597
FaqI GGGAC 4 cut(s) 133, 308, 1076, 1499
FatI CATG 9 cut(s) 525, 662, 943, 990, 1185, 2352, 2496, 2880, 3567
FauI CCCGC 4 cut(s) 423, 1774, 3420, 4107
FauNDI CATATG 2 cut(s) 642, 1299
FbaI TGATCA 1 cut(s) 2251
FblI GTMKAC 1 cut(s) 2729
FriOI GRGCYC 1 cut(s) 194
FspBI CTAG 4 cut(s) 1596, 2054, 2258, 2430
GlaI GCGC 2 cut(s) 212, 4157
GsaI CCCAGC 2 cut(s) 2129, 2408
GsuI CTGGAG 3 cut(s) 62, 110, 2733
HaeII RGCGCY 1 cut(s) 4159
HaeIII GGCC 7 cut(s) 32, 1223, 1785, 2141, 2707, 2843, 3530
HapII CCGG 8 cut(s) 29, 195, 291, 339, 1163, 3302, 3989, 4159
HgaI GACGC 1 cut(s) 1957
HhaI GCGC 2 cut(s) 213, 4158
Hin1II CATG 9 cut(s) 529, 666, 947, 994, 1189, 2356, 2500, 2884, 3571
Hin6I GCGC 2 cut(s) 211, 4156
HinP1I GCGC 2 cut(s) 211, 4156
HincII GTYRAC 1 cut(s) 2730
HindII GTYRAC 1 cut(s) 2730
HpaII CCGG 8 cut(s) 29, 195, 291, 339, 1163, 3302, 3989, 4159
HpyAV CCTTC 5 cut(s) 441, 1008, 1530, 1570, 1904
HpyF10VI GCNNNNNNNGC 9 cut(s) 1640, 1774, 2147, 2210, 2216, 2267, 2401, 2618, 4153
Hsp92II CATG 9 cut(s) 529, 666, 947, 994, 1189, 2356, 2500, 2884, 3571
HspAI GCGC 2 cut(s) 211, 4156
KpnI GGTACC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 2251
LmnI GCTCC 7 cut(s) 197, 1436, 2752, 3184, 3388, 3871, 4075
MabI ACCWGGT 1 cut(s) 570
MaeI CTAG 4 cut(s) 1596, 2054, 2258, 2430
MfeI CAATTG 1 cut(s) 1400
MhlI GDGCHC 5 cut(s) 194, 680, 1312, 3385, 4072
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 2 cut(s) 756, 1664
Mph1103I ATGCAT 1 cut(s) 880
MroXI GAANNNNTTC 2 cut(s) 1580, 2318
MseI TTAA 8 cut(s) 135, 1365, 2132, 2604, 3335, 3837, 4022, 4174
MslI CAYNNNNRTG 4 cut(s) 617, 929, 2108, 2357
MspA1I CMGCKG 3 cut(s) 418, 1562, 1835
MspI CCGG 8 cut(s) 29, 195, 291, 339, 1163, 3302, 3989, 4159
MunI CAATTG 1 cut(s) 1400
Mva1269I GAATGC 3 cut(s) 1762, 1878, 2449
MvaI CCWGG 7 cut(s) 89, 572, 688, 1095, 1458, 1797, 2457
MvnI CGCG 5 cut(s) 211, 3226, 3311, 3913, 3998
MwoI GCNNNNNNNGC 9 cut(s) 1640, 1774, 2147, 2210, 2216, 2267, 2401, 2618, 4153
NciI CCSGG 4 cut(s) 196, 292, 339, 1164
NcoI CCATGG 1 cut(s) 525
NdeI CATATG 2 cut(s) 642, 1299
NheI GCTAGC 1 cut(s) 2257
NlaIII CATG 9 cut(s) 529, 666, 947, 994, 1189, 2356, 2500, 2884, 3571
NmuCI GTSAC 8 cut(s) 68, 280, 380, 1645, 3154, 3269, 3841, 3956
NruI TCGCGA 4 cut(s) 3226, 3311, 3913, 3998
NsiI ATGCAT 1 cut(s) 880
NspI RCATGY 3 cut(s) 666, 994, 1189
NspV TTCGAA 2 cut(s) 1427, 2678
OliI CACNNNNGTG 1 cut(s) 617
PaeI GCATGC 1 cut(s) 1189
PagI TCATGA 1 cut(s) 943
PasI CCCWGGG 1 cut(s) 1094
PctI GAATGC 3 cut(s) 1762, 1878, 2449
PdmI GAANNNNTTC 2 cut(s) 1580, 2318
PflMI CCANNNNNTGG 1 cut(s) 1598
PfoI TCCNGGA 1 cut(s) 87
PleI GAGTC 1 cut(s) 100
PmaCI CACGTG 1 cut(s) 2624
PmlI CACGTG 1 cut(s) 2624
PpsI GAGTC 1 cut(s) 100
Ppu21I YACGTR 3 cut(s) 68, 327, 2624
PpuMI RGGWCCY 1 cut(s) 4127
PsiI TTATAA 1 cut(s) 1673
Psp124BI GAGCTC 1 cut(s) 194
Psp5II RGGWCCY 1 cut(s) 4127
Psp6I CCWGG 7 cut(s) 87, 570, 686, 1093, 1456, 1795, 2455
PspCI CACGTG 1 cut(s) 2624
PspFI CCCAGC 2 cut(s) 2125, 2404
PspGI CCWGG 7 cut(s) 87, 570, 686, 1093, 1456, 1795, 2455
PspPI GGNCC 5 cut(s) 637, 1221, 1783, 2705, 4127
PspPPI RGGWCCY 1 cut(s) 4127
PstI CTGCAG 4 cut(s) 428, 1645, 2968, 3655
PstNI CAGNNNCTG 4 cut(s) 88, 713, 1565, 4130
PvuII CAGCTG 2 cut(s) 1562, 1835
RruI TCGCGA 4 cut(s) 3226, 3311, 3913, 3998
RsaI GTAC 4 cut(s) 332, 764, 2350, 2695
RsaNI GTAC 4 cut(s) 331, 763, 2349, 2694
RseI CAYNNNNRTG 4 cut(s) 617, 929, 2108, 2357
SacI GAGCTC 1 cut(s) 194
SalI GTCGAC 1 cut(s) 2728
SaqAI TTAA 8 cut(s) 135, 1365, 2132, 2604, 3335, 3837, 4022, 4174
Sau96I GGNCC 5 cut(s) 637, 1221, 1783, 2705, 4127
SchI GAGTC 1 cut(s) 101
SduI GDGCHC 5 cut(s) 194, 680, 1312, 3385, 4072
SexAI ACCWGGT 1 cut(s) 570
SfcI CTRYAG 7 cut(s) 424, 535, 1641, 2964, 3036, 3651, 3723
SfuI TTCGAA 2 cut(s) 1427, 2678
SinI GGWCC 2 cut(s) 637, 4127
SmiMI CAYNNNNRTG 4 cut(s) 617, 929, 2108, 2357
SmlI CTYRAG 5 cut(s) 1147, 2947, 3119, 3634, 3806
SmoI CTYRAG 5 cut(s) 1147, 2947, 3119, 3634, 3806
SphI GCATGC 1 cut(s) 1189
SspI AATATT 4 cut(s) 1064, 1361, 1378, 1630
SspMI CTAG 4 cut(s) 1596, 2054, 2258, 2430
SstI GAGCTC 1 cut(s) 194
StyI CCWWGG 2 cut(s) 525, 1224
TaqII GACCGA 2 cut(s) 3381, 4068
TatI WGTACW 1 cut(s) 2693
TauI GCSGC 3 cut(s) 168, 3418, 4105
Tru1I TTAA 8 cut(s) 135, 1365, 2132, 2604, 3335, 3837, 4022, 4174
Tru9I TTAA 8 cut(s) 135, 1365, 2132, 2604, 3335, 3837, 4022, 4174
TscAI CASTG 7 cut(s) 167, 1243, 2025, 2112, 2254, 3385, 4072
TseFI GTSAC 8 cut(s) 68, 280, 380, 1645, 3154, 3269, 3841, 3956
Tsp45I GTSAC 8 cut(s) 68, 280, 380, 1645, 3154, 3269, 3841, 3956
TspGWI ACGGA 2 cut(s) 359, 1076
TspRI CASTG 7 cut(s) 167, 1243, 2025, 2112, 2254, 3385, 4072
Van91I CCANNNNNTGG 1 cut(s) 1598
VneI GTGCAC 1 cut(s) 676
VpaK11BI GGWCC 2 cut(s) 637, 4127
XagI CCTNNNNNAGG 1 cut(s) 4134
XapI RAATTY 6 cut(s) 939, 1336, 1413, 2556, 3103, 3790
XbaI TCTAGA 1 cut(s) 2429
XceI RCATGY 3 cut(s) 666, 994, 1189
XmiI GTMKAC 1 cut(s) 2729
XmnI GAANNNNTTC 2 cut(s) 1580, 2318
XspI CTAG 4 cut(s) 1596, 2054, 2258, 2430
Zsp2I ATGCAT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.