Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0006325.1

Orthogroup ID

OG0013127

Physical Location & Seq

Genomic Coordinates Forward (+)

136497 .. 143255

Gene Structure 6759 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

atgtttacaacttctgacattgactctgaggcagtggagaccactcttgtagccatacaacctagtgtagtgcaagagatgaatgaacagttttgtgcacaatatttcaggaagaagttcggattggttggtaatgaaacacatgtcaatgtttggtctaataattggattccttctttacttgatggtaagcatgaagtgaatgttgtagatctaactaaggaagatcaccaccaccgcgatgccaccgccacagagaagacaccaaaacagccagacttggtcacttgggtccaaccgatagatcctaaccttcaagccgccaccggaaacccctctgaagacgccatcaccgccgcccggatcaccagcccacgccgcccgcgtcagccaacactgacactagagcgccaccggacgagaaaaaactcgcaaaagcctaggcagaagactgctcttctctcaccccagcaagcggctaggtttgttcttgatttcggaattcggaaaatggcgttcagcatcacacaccagatcggagcactggccgggacgccgatatcggtgacggagccgaccacgacctccggcgaatcgacggtggcgagcgtgagcgcgtccgcggtgaggaagtcaccggggacgagcctgacgtgcaaaattcagaggccggaggtgaaggacgggatgctaaccccgccgttgagcccgtgccggtcgccggttctcggggcgacccggccggatctgtccgtggcgtgccaggcgtacgcggcggaggcggaggctccggttttggagcaccaggcgagggccgccggggagagggtaaacggcgtgccggtttttgtaatgatgccgttggacagcgtgacgatgaaccacacggtgaatcggaagaaggcgatgaacgcgagtcttcaggcgttgaagagcgcaggggtggaggggatcatgatggacgtgtggtgggggttggtggagagggacgcgcccggcgcgtacaattggggcggttacgccgagctcttggagatggccaagaaacacggcctcaaagttcaggccgtcatgtcgttccaccagtgcggcggtaacgtcggggactcttgcactattcctttacccagttgggttgtggaggaggttaacaacgatccagaccttgcgtacactgatcaatggggaaataggaactacgagtatctttcacttggcgctgatacacttccggtccttaaagggcgcacgccagtgcaatgttactctgacttcatgcgtgccttcagagataacttcaaacaccttcttggtgacaccattgtggaaatccaagttggaatgggtccagcaggtgagctccgttacccttcatatccagagggggatgggagatggtcatttcctggaattggtgccttccaatgttttgacaagtatatgctgagtagcttaaaagcttattgtgaatctgagggtaagccagaatggggtagcacaggccccactgatgctggtcactacaataactggccggaagataccgccttcttcagaagagaaggtggaggctggaatagtagttacggtgaattcttcctcggctggtactcccagttgctcatggaccatggtgagaggatcctttcctcagccaagtctatctatgagaatgagggtgtgaaaatctcagtaaagattgcaggcatccattggcattatggaacccggtcccacgcccccgagctcacagcagggtactacaacacacgattccgggatggttacctacccattgctcagatgttagcccgccatggtgccgttttcaatttcacttgcatcgagatgcacgaccacgagcagccacaggacgcccaatgttcccctgagaagcttgttaggcaagtggccttggccactcagaaggcaggagtaccacttgcaggtgaaaatgcattgccaaggtatgatgactatgcacacgagcagatcttgcaagcatcagcgttgaacattgagggcaacactgaaggcaagcagatgtgtgcgttcacatacttgaggatgaacccgcaactattccaggccgacaactggaggcgttttgtgtcatttgtgaagaaaatgaaggaaggaaggaacactcaaaagtgtagggaacaggtggagagggaagccgagcattttgtgcacgttacaacacctttggtgcaggaggctgctgttgctctgacgcactga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016052 GO:0016160 GO:0016161 GO:0016787 GO:0016798 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_14

Protein Analysis

747

Amino Acids

83.07

Weight (kDa)

6.26

Isoelectric Point (pI)

37.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_14	PF01373	283 - 706	4.8e-174	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013127)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23920
fragaria_vesca	FvH4_4g05230
malus_domestica	MD12G1073900.v1.1 MD13G1226400.v1.1 MD16G1231500.v1.1
prunus_persica	Prupe.1G053800_v2.0.a1
pyrus_communis	pycom03g22190 pycom13g19950 pycom16g19370
rosa_chinensis	RchiOBHm_Chr4g0396151
rosa_laevigata	RLG00000009565
rosa_multiflora	Rmu_sc0006325.1_g000039
rosa_roxburghii	Rroxscaffold_5G00341000
rosa_rugosa	Rorug03G0351400
rosa_samantha	Rh4BG065100 Rh4CG071900 Rh4DG061900
rosa_wichuraiana	Rw4G005380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1343, 1937
Acc36I	ACCTGC	2 cut(s)	1343, 1937
AccB1I	GGYRCC	2 cut(s)	1415, 1820
AccII	CGCG	8 cut(s)	240, 385, 617, 623, 773, 914, 992, 1001
AclWI	GGATC	7 cut(s)	299, 371, 753, 959, 1151, 1636, 1649
AcoI	YGGCCR	5 cut(s)	546, 740, 1038, 1532, 1917
AcsI	RAATTY	3 cut(s)	501, 660, 1592
AcuI	CTGAAG	5 cut(s)	360, 905, 1270, 1537, 2052
AcyI	GRCGYC	3 cut(s)	345, 554, 1875
AdeI	CACNNNGTG	1 cut(s)	889
AfaI	GTAC	6 cut(s)	770, 1004, 1172, 1610, 1760, 1938
AflIII	ACRYGT	2 cut(s)	142, 963
AgsI	TTSAA	5 cut(s)	317, 931, 1300, 1831, 2014
AjiI	CACGTC	2 cut(s)	654, 964
AjnI	CCWGG	4 cut(s)	762, 804, 1405, 2085
AjuI	GAANNNNNNNTTGG	4 cut(s)	107, 139, 1320, 1352
AleI	CACNNNNGTG	2 cut(s)	1253, 1322
AluBI	AGCT	6 cut(s)	1027, 1360, 1452, 1460, 1747, 1897
AluI	AGCT	6 cut(s)	1027, 1360, 1452, 1460, 1747, 1897
Alw21I	GWGCWC	7 cut(s)	100, 544, 804, 1029, 1362, 1749, 2196
Alw26I	GTCTC	1 cut(s)	32
Alw44I	GTGCAC	2 cut(s)	96, 2192
AlwI	GGATC	7 cut(s)	299, 371, 753, 959, 1151, 1636, 1649
Ama87I	CYCGRG	2 cut(s)	728, 1742
ApaLI	GTGCAC	2 cut(s)	96, 2192
ApeKI	GCWGC	2 cut(s)	1864, 2222
ApoI	RAATTY	3 cut(s)	501, 660, 1592
Asp700I	GAANNNNTTC	1 cut(s)	116
AspA2I	CCTAGG	1 cut(s)	440
AspLEI	GCGC	7 cut(s)	411, 617, 938, 994, 1001, 1220, 1248
AspS9I	GGNCC	7 cut(s)	292, 813, 1234, 1346, 1502, 1627, 1731
AsuC2I	CCSGG	8 cut(s)	361, 550, 639, 739, 820, 996, 1729, 1778
AvaI	CYCGRG	2 cut(s)	728, 1742
AvaII	GGWCC	5 cut(s)	292, 1234, 1346, 1627, 1731
AvrII	CCTAGG	1 cut(s)	440
BaeGI	GKGCMC	2 cut(s)	100, 2196
BalI	TGGCCA	2 cut(s)	1040, 1919
BamHI	GGATCC	1 cut(s)	1641
BanI	GGYRCC	2 cut(s)	1415, 1820
BanII	GRGCYC	4 cut(s)	710, 1029, 1362, 1749
BauI	CACGAG	2 cut(s)	1859, 1985
BbsI	GAAGAC	4 cut(s)	266, 348, 455, 911
Bbv12I	GWGCWC	7 cut(s)	100, 544, 804, 1029, 1362, 1749, 2196
BbvCI	CCTCAGC	1 cut(s)	1651
BbvI	GCAGC	2 cut(s)	1876, 2209
BccI	CCATC	7 cut(s)	179, 356, 952, 1030, 1382, 1389, 1775
BceAI	ACGGC	6 cut(s)	685, 844, 850, 1052, 1066, 1808
BciT130I	CCWGG	4 cut(s)	764, 806, 1407, 2087
BclI	TGATCA	1 cut(s)	1177
BcnI	CCSGG	8 cut(s)	361, 550, 639, 739, 820, 996, 1729, 1778
BcoDI	GTCTC	1 cut(s)	32
BfaI	CTAG	4 cut(s)	63, 404, 441, 480
BfoI	RGCGCY	2 cut(s)	412, 1221
BfuAI	ACCTGC	2 cut(s)	1343, 1937
BglII	AGATCT	2 cut(s)	211, 1992
BisI	GCNGC	9 cut(s)	321, 357, 379, 477, 774, 816, 1090, 1865, 2223
BlnI	CCTAGG	1 cut(s)	440
BlsI	GCNGC	9 cut(s)	322, 358, 380, 478, 775, 817, 1091, 1866, 2224
Bme18I	GGWCC	5 cut(s)	292, 1234, 1346, 1627, 1731
BmeT110I	CYCGRG	2 cut(s)	728, 1742
BmgBI	CACGTC	2 cut(s)	654, 964
BmgT120I	GGNCC	7 cut(s)	292, 813, 1234, 1346, 1502, 1627, 1731
BmrI	ACTGGG	2 cut(s)	1122, 1609
BmsI	GCATC	9 cut(s)	232, 531, 678, 846, 1501, 1716, 1839, 1851, 2012
BmuI	ACTGGG	2 cut(s)	1122, 1609
BpiI	GAAGAC	4 cut(s)	266, 348, 455, 911
BpmI	CTGGAG	1 cut(s)	2119
Bpu10I	CCTNAGC	1 cut(s)	1651
BpuEI	CTTGAG	1 cut(s)	2083
BpuMI	CCSGG	8 cut(s)	361, 550, 639, 739, 820, 996, 1729, 1778
BsaHI	GRCGYC	3 cut(s)	345, 554, 1875
BsaI	GGTCTC	1 cut(s)	32
BsaWI	WCCGGW	4 cut(s)	326, 414, 790, 1231
Bse118I	RCCGGY	3 cut(s)	714, 721, 841
Bse1I	ACTGG	7 cut(s)	549, 1084, 1128, 1253, 1535, 1615, 2102
Bse3DI	GCAATG	3 cut(s)	1265, 1794, 1958
BseBI	CCWGG	4 cut(s)	764, 806, 1407, 2087
BseGI	GGATG	5 cut(s)	693, 1393, 1707, 1786, 2073
BseMI	GCAATG	3 cut(s)	1265, 1794, 1958
BseMII	CTCAG	8 cut(s)	18, 1436, 1464, 1665, 1704, 1814, 1881, 1937
BseNI	ACTGG	7 cut(s)	549, 1084, 1128, 1253, 1535, 1615, 2102
BseRI	GAGGAG	1 cut(s)	1157
BseSI	GKGCMC	2 cut(s)	100, 2196
BseX3I	CGGCCG	1 cut(s)	740
BseXI	GCAGC	2 cut(s)	1876, 2209
BseYI	CCCAGC	1 cut(s)	468
BsgI	GTGCAG	1 cut(s)	2234
Bsh1236I	CGCG	8 cut(s)	240, 385, 617, 623, 773, 914, 992, 1001
Bsh1285I	CGRYCG	2 cut(s)	719, 743
BshNI	GGYRCC	2 cut(s)	1415, 1820
BsiEI	CGRYCG	2 cut(s)	719, 743
BsiHKAI	GWGCWC	7 cut(s)	100, 544, 804, 1029, 1362, 1749, 2196
BsiHKCI	CYCGRG	2 cut(s)	728, 1742
BsiWI	CGTACG	1 cut(s)	768
BslFI	GGGAC	5 cut(s)	565, 655, 1001, 1118, 1717
BsmAI	GTCTC	1 cut(s)	32
BsmFI	GGGAC	5 cut(s)	565, 655, 1001, 1118, 1717
Bso31I	GGTCTC	1 cut(s)	32
BsoBI	CYCGRG	2 cut(s)	728, 1742
Bsp1286I	GDGCHC	8 cut(s)	100, 544, 710, 804, 1029, 1362, 1749, 2196
Bsp19I	CCATGG	2 cut(s)	1630, 1816
BspCNI	CTCAG	8 cut(s)	19, 1437, 1465, 1664, 1703, 1813, 1882, 1936
BspFNI	CGCG	8 cut(s)	240, 385, 617, 623, 773, 914, 992, 1001
BspHI	TCATGA	1 cut(s)	954
BspMI	ACCTGC	2 cut(s)	1343, 1937
BspPI	GGATC	7 cut(s)	299, 371, 753, 959, 1151, 1636, 1649
BspQI	GCTCTTC	2 cut(s)	462, 926
BspT107I	GGYRCC	2 cut(s)	1415, 1820
BspTNI	GGTCTC	1 cut(s)	32
BsrDI	GCAATG	3 cut(s)	1265, 1794, 1958
BsrFI	RCCGGY	3 cut(s)	714, 721, 841
BsrI	ACTGG	7 cut(s)	549, 1084, 1128, 1253, 1535, 1615, 2102
BssAI	RCCGGY	3 cut(s)	714, 721, 841
BssNI	GRCGYC	3 cut(s)	345, 554, 1875
BssSI	CACGAG	2 cut(s)	1859, 1985
BssT1I	CCWWGG	5 cut(s)	440, 1630, 1816, 1914, 1964
Bst2BI	CACGAG	2 cut(s)	1859, 1985
Bst2UI	CCWGG	4 cut(s)	764, 806, 1407, 2087
Bst4CI	ACNGT	4 cut(s)	90, 601, 889, 1589
Bst6I	CTCTTC	3 cut(s)	462, 926, 1552
BstACI	GRCGYC	3 cut(s)	345, 554, 1875
BstAPI	GCANNNNNTGC	2 cut(s)	1996, 2191
BstDEI	CTNAG	9 cut(s)	27, 219, 1445, 1473, 1651, 1690, 1800, 1890, 1923
BstDSI	CCRYGG	4 cut(s)	621, 753, 1630, 1816
BstEII	GGTNACC	1 cut(s)	1784
BstF5I	GGATG	5 cut(s)	693, 1393, 1707, 1786, 2073
BstFNI	CGCG	8 cut(s)	240, 385, 617, 623, 773, 914, 992, 1001
BstH2I	RGCGCY	2 cut(s)	412, 1221
BstHHI	GCGC	7 cut(s)	411, 617, 938, 994, 1001, 1220, 1248
BstMAI	GTCTC	1 cut(s)	32
BstMCI	CGRYCG	2 cut(s)	719, 743
BstNI	CCWGG	4 cut(s)	764, 806, 1407, 2087
BstNSI	RCATGY	1 cut(s)	146
BstPI	GGTNACC	1 cut(s)	1784
BstSLI	GKGCMC	2 cut(s)	100, 2196
BstUI	CGCG	8 cut(s)	240, 385, 617, 623, 773, 914, 992, 1001
BstV1I	GCAGC	2 cut(s)	1876, 2209
BstV2I	GAAGAC	4 cut(s)	266, 348, 455, 911
BstX2I	RGATCY	5 cut(s)	211, 304, 745, 1641, 1992
BstYI	RGATCY	5 cut(s)	211, 304, 745, 1641, 1992
BstZI	CGGCCG	1 cut(s)	740
BtgI	CCRYGG	4 cut(s)	621, 753, 1630, 1816
BtgZI	GCGATG	2 cut(s)	255, 920
BtrI	CACGTC	2 cut(s)	654, 964
BtsCI	GGATG	5 cut(s)	693, 1393, 1707, 1786, 2073
BtsI	GCAGTG	1 cut(s)	39
BtsIMutI	CAGTG	9 cut(s)	39, 395, 542, 1091, 1173, 1260, 1506, 2028, 2239
BveI	ACCTGC	2 cut(s)	1343, 1937
CciI	TCATGA	1 cut(s)	954
CfoI	GCGC	7 cut(s)	411, 617, 938, 994, 1001, 1220, 1248
Cfr10I	RCCGGY	3 cut(s)	714, 721, 841
Cfr13I	GGNCC	7 cut(s)	292, 813, 1234, 1346, 1502, 1627, 1731
Cfr42I	CCGCGG	1 cut(s)	624
CseI	GACGC	6 cut(s)	353, 374, 562, 606, 998, 1883
Csp6I	GTAC	6 cut(s)	769, 1003, 1171, 1609, 1759, 1937
CviAII	CATG	8 cut(s)	143, 194, 955, 1072, 1276, 1624, 1631, 1817
CviQI	GTAC	6 cut(s)	769, 1003, 1171, 1609, 1759, 1937
DdeI	CTNAG	9 cut(s)	27, 219, 1445, 1473, 1651, 1690, 1800, 1890, 1923
DraIII	CACNNNGTG	1 cut(s)	889
EaeI	YGGCCR	5 cut(s)	546, 740, 1038, 1532, 1917
EagI	CGGCCG	1 cut(s)	740
Eam1104I	CTCTTC	3 cut(s)	462, 926, 1552
EarI	CTCTTC	3 cut(s)	462, 926, 1552
EciI	GGCGGA	2 cut(s)	791, 797
Ecl136II	GAGCTC	3 cut(s)	1027, 1360, 1747
EclXI	CGGCCG	1 cut(s)	740
Eco130I	CCWWGG	5 cut(s)	440, 1630, 1816, 1914, 1964
Eco24I	GRGCYC	4 cut(s)	710, 1029, 1362, 1749
Eco31I	GGTCTC	1 cut(s)	32
Eco32I	GATATC	1 cut(s)	561
Eco47I	GGWCC	5 cut(s)	292, 1234, 1346, 1627, 1731
Eco52I	CGGCCG	1 cut(s)	740
Eco53kI	GAGCTC	3 cut(s)	1027, 1360, 1747
Eco57I	CTGAAG	5 cut(s)	360, 905, 1270, 1537, 2052
Eco88I	CYCGRG	2 cut(s)	728, 1742
Eco91I	GGTNACC	1 cut(s)	1784
EcoICRI	GAGCTC	3 cut(s)	1027, 1360, 1747
EcoO109I	RGGNCCY	1 cut(s)	1502
EcoO65I	GGTNACC	1 cut(s)	1784
EcoRI	GAATTC	2 cut(s)	501, 1592
EcoRII	CCWGG	4 cut(s)	762, 804, 1405, 2085
EcoRV	GATATC	1 cut(s)	561
EcoT14I	CCWWGG	5 cut(s)	440, 1630, 1816, 1914, 1964
EcoT22I	ATGCAT	1 cut(s)	1960
EcoT38I	GRGCYC	4 cut(s)	710, 1029, 1362, 1749
ErhI	CCWWGG	5 cut(s)	440, 1630, 1816, 1914, 1964
FaeI	CATG	8 cut(s)	146, 197, 958, 1075, 1279, 1627, 1634, 1820
FaqI	GGGAC	5 cut(s)	565, 655, 1001, 1118, 1717
FatI	CATG	8 cut(s)	142, 193, 954, 1071, 1275, 1623, 1630, 1816
FauI	CCCGC	4 cut(s)	390, 705, 1820, 2082
FbaI	TGATCA	1 cut(s)	1177
Fnu4HI	GCNGC	9 cut(s)	321, 357, 379, 477, 774, 816, 1090, 1865, 2223
FokI	GGATG	5 cut(s)	700, 1400, 1694, 1793, 2080
FriOI	GRGCYC	4 cut(s)	710, 1029, 1362, 1749
Fsp4HI	GCNGC	9 cut(s)	321, 357, 379, 477, 774, 816, 1090, 1865, 2223
FspBI	CTAG	4 cut(s)	63, 404, 441, 480
GlaI	GCGC	7 cut(s)	410, 616, 937, 993, 1000, 1219, 1247
GluI	GCNGC	9 cut(s)	321, 357, 379, 477, 774, 816, 1090, 1865, 2223
GsaI	CCCAGC	1 cut(s)	472
GsuI	CTGGAG	1 cut(s)	2119
HaeII	RGCGCY	2 cut(s)	412, 1221
HgaI	GACGC	6 cut(s)	353, 374, 562, 606, 998, 1883
HhaI	GCGC	7 cut(s)	411, 617, 938, 994, 1001, 1220, 1248
Hin1I	GRCGYC	3 cut(s)	345, 554, 1875
Hin1II	CATG	8 cut(s)	146, 197, 958, 1075, 1279, 1627, 1634, 1820
Hin6I	GCGC	7 cut(s)	409, 615, 936, 992, 999, 1218, 1246
HinP1I	GCGC	7 cut(s)	409, 615, 936, 992, 999, 1218, 1246
HincII	GTYRAC	1 cut(s)	1150
HindII	GTYRAC	1 cut(s)	1150
HindIII	AAGCTT	2 cut(s)	1458, 1895
HinfI	GANTC	8 cut(s)	23, 169, 593, 892, 916, 1106, 1469, 1773
HpaI	GTTAAC	1 cut(s)	1150
Hpy166II	GTNNAC	7 cut(s)	6, 98, 832, 1150, 1173, 2055, 2194
Hpy188III	TCNNGA	6 cut(s)	109, 491, 955, 1160, 1379, 1846
Hpy8I	GTNNAC	7 cut(s)	6, 98, 832, 1150, 1173, 2055, 2194
Hpy99I	CGWCG	2 cut(s)	601, 1103
HpyCH4III	ACNGT	4 cut(s)	90, 601, 889, 1589
HpyCH4IV	ACGT	4 cut(s)	653, 963, 1098, 2196
HpyF3I	CTNAG	9 cut(s)	27, 219, 1445, 1473, 1651, 1690, 1800, 1890, 1923
HpySE526I	ACGT	4 cut(s)	653, 963, 1098, 2196
Hsp92I	GRCGYC	3 cut(s)	345, 554, 1875
Hsp92II	CATG	8 cut(s)	146, 197, 958, 1075, 1279, 1627, 1634, 1820
HspAI	GCGC	7 cut(s)	409, 615, 936, 992, 999, 1218, 1246
Ksp22I	TGATCA	1 cut(s)	1177
KspAI	GTTAAC	1 cut(s)	1150
KspI	CCGCGG	1 cut(s)	624
LguI	GCTCTTC	2 cut(s)	462, 926
LmnI	GCTCC	5 cut(s)	539, 571, 793, 799, 1365
Lsp1109I	GCAGC	2 cut(s)	1876, 2209
LweI	GCATC	9 cut(s)	232, 531, 678, 846, 1501, 1716, 1839, 1851, 2012
MaeI	CTAG	4 cut(s)	63, 404, 441, 480
MaeII	ACGT	4 cut(s)	653, 963, 1098, 2196
MfeI	CAATTG	1 cut(s)	1006
MflI	RGATCY	5 cut(s)	211, 304, 745, 1641, 1992
MhlI	GDGCHC	8 cut(s)	100, 544, 710, 804, 1029, 1362, 1749, 2196
MlsI	TGGCCA	2 cut(s)	1040, 1919
MluCI	AATT	7 cut(s)	163, 501, 660, 1006, 1410, 1592, 1831
MluNI	TGGCCA	2 cut(s)	1040, 1919
MlyI	GAGTC	3 cut(s)	17, 925, 1100
MmeI	TCCRAC	3 cut(s)	319, 843, 1318
Mox20I	TGGCCA	2 cut(s)	1040, 1919
Mph1103I	ATGCAT	1 cut(s)	1960
MroXI	GAANNNNTTC	1 cut(s)	116
MscI	TGGCCA	2 cut(s)	1040, 1919
MseI	TTAA	3 cut(s)	1149, 1239, 1454
MslI	CAYNNNNRTG	5 cut(s)	147, 240, 1253, 1322, 1847
Msp20I	TGGCCA	2 cut(s)	1040, 1919
MspA1I	CMGCKG	1 cut(s)	623
MunI	CAATTG	1 cut(s)	1006
MvaI	CCWGG	4 cut(s)	764, 806, 1407, 2087
MvnI	CGCG	8 cut(s)	240, 385, 617, 623, 773, 914, 992, 1001
NciI	CCSGG	8 cut(s)	361, 550, 639, 739, 820, 996, 1729, 1778
NcoI	CCATGG	2 cut(s)	1630, 1816
NlaIII	CATG	8 cut(s)	146, 197, 958, 1075, 1279, 1627, 1634, 1820
NmeAIII	GCCGAG	3 cut(s)	1048, 1581, 2206
NmuCI	GTSAC	6 cut(s)	283, 565, 633, 871, 1313, 1517
NsiI	ATGCAT	1 cut(s)	1960
NspI	RCATGY	1 cut(s)	146
OliI	CACNNNNGTG	2 cut(s)	1253, 1322
PagI	TCATGA	1 cut(s)	954
PaqCI	CACCTGC	2 cut(s)	1343, 1937
PciI	ACATGT	1 cut(s)	142
PciSI	GCTCTTC	2 cut(s)	462, 926
PcsI	WCGNNNNNNNCGW	4 cut(s)	382, 602, 650, 1851
PdmI	GAANNNNTTC	1 cut(s)	116
PfeI	GAWTC	5 cut(s)	169, 593, 892, 1469, 1773
Pfl23II	CGTACG	1 cut(s)	768
PflFI	GACNNNGTC	1 cut(s)	281
PfoI	TCCNGGA	2 cut(s)	1405, 1776
PkrI	GCNGC	9 cut(s)	322, 358, 380, 478, 775, 817, 1091, 1866, 2224
PleI	GAGTC	3 cut(s)	17, 924, 1100
PpsI	GAGTC	3 cut(s)	17, 924, 1100
PscI	ACATGT	1 cut(s)	142
Psp124BI	GAGCTC	3 cut(s)	1029, 1362, 1749
Psp6I	CCWGG	4 cut(s)	762, 804, 1405, 2085
PspEI	GGTNACC	1 cut(s)	1784
PspFI	CCCAGC	1 cut(s)	468
PspGI	CCWGG	4 cut(s)	762, 804, 1405, 2085
PspLI	CGTACG	1 cut(s)	768
PspPI	GGNCC	7 cut(s)	292, 813, 1234, 1346, 1502, 1627, 1731
PsuI	RGATCY	5 cut(s)	211, 304, 745, 1641, 1992
PsyI	GACNNNGTC	1 cut(s)	281
RsaI	GTAC	6 cut(s)	770, 1004, 1172, 1610, 1760, 1938
RsaNI	GTAC	6 cut(s)	769, 1003, 1171, 1609, 1759, 1937
RseI	CAYNNNNRTG	5 cut(s)	147, 240, 1253, 1322, 1847
SacI	GAGCTC	3 cut(s)	1029, 1362, 1749
SacII	CCGCGG	1 cut(s)	624
SapI	GCTCTTC	2 cut(s)	462, 926
SaqAI	TTAA	3 cut(s)	1149, 1239, 1454
SatI	GCNGC	9 cut(s)	321, 357, 379, 477, 774, 816, 1090, 1865, 2223
Sau96I	GGNCC	7 cut(s)	292, 813, 1234, 1346, 1502, 1627, 1731
SchI	GAGTC	3 cut(s)	17, 925, 1100
SduI	GDGCHC	8 cut(s)	100, 544, 710, 804, 1029, 1362, 1749, 2196
SfaNI	GCATC	9 cut(s)	232, 531, 678, 846, 1501, 1716, 1839, 1851, 2012
Sfr303I	CCGCGG	1 cut(s)	624
SgrBI	CCGCGG	1 cut(s)	624
SinI	GGWCC	5 cut(s)	292, 1234, 1346, 1627, 1731
SmiMI	CAYNNNNRTG	5 cut(s)	147, 240, 1253, 1322, 1847
SmlI	CTYRAG	1 cut(s)	2062
SmoI	CTYRAG	1 cut(s)	2062
Sse9I	AATT	7 cut(s)	163, 501, 660, 1006, 1410, 1592, 1831
SspI	AATATT	1 cut(s)	104
SspMI	CTAG	4 cut(s)	63, 404, 441, 480
SstI	GAGCTC	3 cut(s)	1029, 1362, 1749
StyI	CCWWGG	5 cut(s)	440, 1630, 1816, 1914, 1964
TaaI	ACNGT	4 cut(s)	90, 601, 889, 1589
TaiI	ACGT	4 cut(s)	656, 966, 1101, 2199
TaqI	TCGA	2 cut(s)	596, 1845
TasI	AATT	7 cut(s)	163, 501, 660, 1006, 1410, 1592, 1831
TauI	GCSGC	7 cut(s)	323, 359, 381, 479, 776, 818, 1092
TfiI	GAWTC	5 cut(s)	169, 593, 892, 1469, 1773
Tru1I	TTAA	3 cut(s)	1149, 1239, 1454
Tru9I	TTAA	3 cut(s)	1149, 1239, 1454
TscAI	CASTG	8 cut(s)	39, 402, 549, 1091, 1180, 1260, 1513, 2035
TseFI	GTSAC	6 cut(s)	283, 565, 633, 871, 1313, 1517
TseI	GCWGC	2 cut(s)	1864, 2222
Tsp45I	GTSAC	6 cut(s)	283, 565, 633, 871, 1313, 1517
TspGWI	ACGGA	3 cut(s)	584, 742, 1352
TspRI	CASTG	8 cut(s)	39, 402, 549, 1091, 1180, 1260, 1513, 2035
Tth111I	GACNNNGTC	1 cut(s)	281
VneI	GTGCAC	2 cut(s)	96, 2192
VpaK11BI	GGWCC	5 cut(s)	292, 1234, 1346, 1627, 1731
XapI	RAATTY	3 cut(s)	501, 660, 1592
XceI	RCATGY	1 cut(s)	146
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1135, 1718
XmaJI	CCTAGG	1 cut(s)	440
XmnI	GAANNNNTTC	1 cut(s)	116
XspI	CTAG	4 cut(s)	63, 404, 441, 480
Zsp2I	ATGCAT	1 cut(s)	1960

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.