Rmu_sc0007459.1_g000001

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007459.1
OG0017325
Physical Location & Seq
Forward (+)
41 .. 1279
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1239 bp
atgaaaggtatgaagaaagatgatgcgaaggtttttggcttggggattgatttgaagggaggtgagattcatggccgggttttgaagctggggtttgattccgacttgtatgttcagaattcgttgctgttcgtatatgctcaagctgggtctgtgggactcgcgcgccaggtgttcgatgaaatgtctgagagaagcgtttcgtcgtggaacatcatgatatcagcttatgatcagataacggatttcgaatcggcggactggctgttccagttgatgcctcagaagaatgtggtgtcttggaatacattattagggcggcatgttagattgggcaacgttgaggctgcgaagatggtgtttgaagagatggtggagagggatgcggtttcgtggaattcgatgattgctggttatgttcaggttagagattatggtggagcattggagctgttccgggaaatgcaagttgctgaagtggaagctacggaagtgacgcttatatcggttttgggtgcttgtgctgaaaccggggagttggaggttggaaggaagattcatgagtcgttgaaaggaaagcagcataagattgaagggtacttggggactgcccttgtggatatgtattctaaatgtggcaagttgagttcagcttgggaggtgtttagtgggatgaaaatgagaccggttggttgctggaatgcaatgattatgggtttcgctgtccatggtcactgcgaggaagctttggagttgtttgcagatatggaaatgcaggttggtgaagtgaagccgaatcggatcacctttgttggggtgctcattgcttgtagtcacaagggtttggtggaagaagggcgccggtatttcagttacatgattcaaaaatacaaaatcgtgcctgatactaagcattatggttgcatggttgatcttcttagcaggtggggattgttagatgaagcctaccagatgatcaaagctgtgccttccctatcaagctctttgttgtggagaaccttgttgggtgcttgtaaggtacatggaaacgttgaattggctgagcaatgctttgagcagctagccaagttggaacctcttaaggatgcagattatgttttgttgtcaaacatctacgccgaagccgagaaatggcacgatgtcgagcggttgaggaatgagatggtttgtaagggtgttcccaaaagcctcggcttcagccatgtagagatgaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

412

Amino Acids

46.3

Weight (kDa)

5.65

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 96 - 127 1.5e-06 PPR repeat family
PPR_2 PF13041 128 - 175 1.5e-08 PPR repeat family
PPR PF01535 130 - 156 4.7e-07 PPR repeat
PPR_2 PF13041 231 - 277 1.9e-06 PPR repeat family
PPR PF01535 232 - 257 1.6e-06 PPR repeat
E_motif PF20431 348 - 410 3.2e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017325)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g10030
malus_domestica MD04G1173200.v1.1 MD12G1186600.v1.1
prunus_persica Prupe.6G292100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0460711
rosa_laevigata RLG00000024986
rosa_multiflora Rmu_sc0007459.1_g000001 Rmu_sc0011069.1_g000007
rosa_roxburghii Rroxscaffold_6G00419280
rosa_rugosa Rorug03G0045400
rosa_samantha Rh3AG102900 Rh3DG108100
rosa_wichuraiana Rw3G008760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 933
Acc36I ACCTGC 2 cut(s) 766, 933
AccB1I GGYRCC 1 cut(s) 858
AccBSI CCGCTC 1 cut(s) 1168
AccII CGCG 2 cut(s) 164, 166
AciI CCGC 4 cut(s) 257, 319, 386, 1168
AclI AACGTT 2 cut(s) 339, 1050
AclWI GGATC 1 cut(s) 809
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 2 cut(s) 118, 397
AcuI CTGAAG 2 cut(s) 495, 1201
AcyI GRCGYC 1 cut(s) 859
AfaI GTAC 2 cut(s) 599, 1041
AfiI CCNNNNNNNGG 2 cut(s) 813, 1152
AflII CTTAAG 1 cut(s) 1100
AgeI ACCGGT 1 cut(s) 685
AgsI TTSAA 7 cut(s) 55, 85, 365, 571, 593, 884, 1055
AjnI CCWGG 1 cut(s) 168
AjuI GAANNNNNNNTTGG 2 cut(s) 762, 794
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
Alw21I GWGCWC 1 cut(s) 822
Alw26I GTCTC 1 cut(s) 676
AlwI GGATC 1 cut(s) 809
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 3 cut(s) 347, 580, 1078
ApoI RAATTY 2 cut(s) 118, 397
AsiGI ACCGGT 1 cut(s) 685
Asp700I GAANNNNTTC 1 cut(s) 199
AspLEI GCGC 3 cut(s) 166, 168, 861
AsuC2I CCSGG 3 cut(s) 77, 458, 532
AsuHPI GGTGA 3 cut(s) 74, 794, 796
AsuII TTCGAA 1 cut(s) 249
AsuNHI GCTAGC 1 cut(s) 1081
BanI GGYRCC 1 cut(s) 858
Bbv12I GWGCWC 1 cut(s) 822
BbvI GCAGC 3 cut(s) 334, 592, 1090
BccI CCATC 3 cut(s) 349, 364, 1177
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 2 cut(s) 232, 975
BcnI CCSGG 3 cut(s) 77, 458, 532
BcoDI GTCTC 1 cut(s) 676
BfaI CTAG 1 cut(s) 1082
BfoI RGCGCY 1 cut(s) 862
BfrI CTTAAG 1 cut(s) 1100
BfuAI ACCTGC 2 cut(s) 766, 933
BisI GCNGC 4 cut(s) 320, 348, 581, 1079
BlpI GCTNAGC 1 cut(s) 1062
BlsI GCNGC 4 cut(s) 321, 349, 582, 1080
Bme1390I CCNGG 4 cut(s) 77, 170, 458, 532
BmiI GGNNCC 2 cut(s) 860, 1095
BmrFI CCNGG 4 cut(s) 77, 170, 458, 532
BmsI GCATC 4 cut(s) 13, 267, 373, 1096
BmtI GCTAGC 1 cut(s) 1085
Bpu1102I GCTNAGC 1 cut(s) 1062
Bpu14I TTCGAA 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 126
BpuMI CCSGG 3 cut(s) 77, 458, 532
BsaHI GRCGYC 1 cut(s) 859
BsaI GGTCTC 1 cut(s) 676
BsaJI CCNNGG 3 cut(s) 531, 727, 1210
BsaWI WCCGGW 1 cut(s) 685
Bsc4I CCNNNNNNNGG 2 cut(s) 813, 1152
Bse118I RCCGGY 2 cut(s) 685, 861
Bse1I ACTGG 2 cut(s) 266, 271
Bse3DI GCAATG 3 cut(s) 711, 822, 1073
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 3 cut(s) 531, 727, 1210
BseGI GGATG 3 cut(s) 388, 678, 1111
BseLI CCNNNNNNNGG 2 cut(s) 813, 1152
BseMI GCAATG 3 cut(s) 711, 822, 1073
BseMII CTCAG 3 cut(s) 180, 296, 1053
BseNI ACTGG 2 cut(s) 266, 271
BsePI GCGCGC 1 cut(s) 164
BseXI GCAGC 3 cut(s) 334, 592, 1090
BseYI CCCAGC 2 cut(s) 88, 146
Bsh1236I CGCG 2 cut(s) 164, 166
BshFI GGCC 1 cut(s) 75
BshNI GGYRCC 1 cut(s) 858
BshTI ACCGGT 1 cut(s) 685
BsiHKAI GWGCWC 1 cut(s) 822
BsiSI CCGG 5 cut(s) 76, 457, 531, 686, 862
BslFI GGGAC 2 cut(s) 171, 619
BslI CCNNNNNNNGG 2 cut(s) 813, 1152
BsmAI GTCTC 1 cut(s) 676
BsmFI GGGAC 2 cut(s) 171, 619
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 1 cut(s) 75
Bso31I GGTCTC 1 cut(s) 676
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 4 cut(s) 232, 801, 931, 975
Bsp1720I GCTNAGC 1 cut(s) 1062
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 4 cut(s) 257, 319, 386, 1168
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 3 cut(s) 181, 295, 1054
BspFNI CGCG 2 cut(s) 164, 166
BspHI TCATGA 2 cut(s) 216, 559
BspLI GGNNCC 2 cut(s) 860, 1095
BspMI ACCTGC 2 cut(s) 766, 933
BspOI GCTAGC 1 cut(s) 1085
BspPI GGATC 1 cut(s) 809
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 858
BspTI CTTAAG 1 cut(s) 1100
BspTNI GGTCTC 1 cut(s) 676
BsrBI CCGCTC 1 cut(s) 1168
BsrDI GCAATG 3 cut(s) 711, 822, 1073
BsrFI RCCGGY 2 cut(s) 685, 861
BsrI ACTGG 2 cut(s) 266, 271
BssAI RCCGGY 2 cut(s) 685, 861
BssECI CCNNGG 3 cut(s) 531, 727, 1210
BssHII GCGCGC 1 cut(s) 164
BssMI GATC 4 cut(s) 232, 801, 931, 975
BssNI GRCGYC 1 cut(s) 859
BssT1I CCWWGG 1 cut(s) 727
Bst2UI CCWGG 1 cut(s) 170
Bst6I CTCTTC 1 cut(s) 360
BstACI GRCGYC 1 cut(s) 859
BstAFI CTTAAG 1 cut(s) 1100
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 166, 1083
BstDEI CTNAG 5 cut(s) 189, 282, 909, 938, 1062
BstDSI CCRYGG 1 cut(s) 727
BstF5I GGATG 3 cut(s) 388, 678, 1111
BstFNI CGCG 2 cut(s) 164, 166
BstH2I RGCGCY 1 cut(s) 862
BstHHI GCGC 3 cut(s) 166, 168, 861
BstKTI GATC 4 cut(s) 235, 804, 934, 978
BstMAI GTCTC 1 cut(s) 676
BstMBI GATC 4 cut(s) 232, 801, 931, 975
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 4 cut(s) 75, 168, 456, 530
BstUI CGCG 2 cut(s) 164, 166
BstV1I GCAGC 3 cut(s) 334, 592, 1090
BsuRI GGCC 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 727
BtsCI GGATG 3 cut(s) 388, 678, 1111
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 1 cut(s) 733
BveI ACCTGC 2 cut(s) 766, 933
Cac8I GCNNGC 2 cut(s) 166, 1083
CciI TCATGA 2 cut(s) 216, 559
CfoI GCGC 3 cut(s) 166, 168, 861
Cfr10I RCCGGY 2 cut(s) 685, 861
CseI GACGC 1 cut(s) 505
Csp6I GTAC 2 cut(s) 598, 1040
CspAI ACCGGT 1 cut(s) 685
CviAII CATG 9 cut(s) 71, 217, 323, 560, 728, 877, 925, 1043, 1223
CviQI GTAC 2 cut(s) 598, 1040
DdeI CTNAG 5 cut(s) 189, 282, 909, 938, 1062
DinI GGCGCC 1 cut(s) 860
DpnI GATC 4 cut(s) 234, 803, 933, 977
DpnII GATC 4 cut(s) 232, 801, 931, 975
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
EciI GGCGGA 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 727
Eco31I GGTCTC 1 cut(s) 676
Eco32I GATATC 1 cut(s) 222
Eco57I CTGAAG 2 cut(s) 495, 1201
EcoRI GAATTC 2 cut(s) 118, 397
EcoRII CCWGG 1 cut(s) 168
EcoRV GATATC 1 cut(s) 222
EcoT14I CCWWGG 1 cut(s) 727
EgeI GGCGCC 1 cut(s) 860
EheI GGCGCC 1 cut(s) 860
ErhI CCWWGG 1 cut(s) 727
FaeI CATG 9 cut(s) 74, 220, 326, 563, 731, 880, 928, 1046, 1226
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 2 cut(s) 171, 619
FatI CATG 9 cut(s) 70, 216, 322, 559, 727, 876, 924, 1042, 1222
FbaI TGATCA 2 cut(s) 232, 975
Fnu4HI GCNGC 4 cut(s) 320, 348, 581, 1079
FokI GGATG 3 cut(s) 395, 685, 1118
Fsp4HI GCNGC 4 cut(s) 320, 348, 581, 1079
FspBI CTAG 1 cut(s) 1082
GlaI GCGC 3 cut(s) 165, 167, 860
GluI GCNGC 4 cut(s) 320, 348, 581, 1079
GsaI CCCAGC 2 cut(s) 92, 150
HaeII RGCGCY 1 cut(s) 862
HaeIII GGCC 1 cut(s) 75
HapII CCGG 5 cut(s) 76, 457, 531, 686, 862
HgaI GACGC 1 cut(s) 505
HhaI GCGC 3 cut(s) 166, 168, 861
Hin1I GRCGYC 1 cut(s) 859
Hin1II CATG 9 cut(s) 74, 220, 326, 563, 731, 880, 928, 1046, 1226
Hin6I GCGC 3 cut(s) 164, 166, 859
HinP1I GCGC 3 cut(s) 164, 166, 859
HindIII AAGCTT 1 cut(s) 744
HinfI GANTC 8 cut(s) 67, 98, 159, 251, 556, 563, 796, 880
HpaII CCGG 5 cut(s) 76, 457, 531, 686, 862
HphI GGTGA 3 cut(s) 74, 794, 796
Hpy188I TCNGA 6 cut(s) 103, 117, 190, 237, 285, 801
Hpy188III TCNNGA 2 cut(s) 217, 560
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 6 cut(s) 22, 49, 543, 587, 848, 999
HpyCH4IV ACGT 2 cut(s) 339, 1050
HpyCH4V TGCA 6 cut(s) 466, 704, 761, 775, 924, 1109
HpyF3I CTNAG 5 cut(s) 189, 282, 909, 938, 1062
HpySE526I ACGT 2 cut(s) 339, 1050
Hsp92I GRCGYC 1 cut(s) 859
Hsp92II CATG 9 cut(s) 74, 220, 326, 563, 731, 880, 928, 1046, 1226
HspAI GCGC 3 cut(s) 164, 166, 859
KasI GGCGCC 1 cut(s) 858
Ksp22I TGATCA 2 cut(s) 232, 975
Kzo9I GATC 4 cut(s) 232, 801, 931, 975
LmnI GCTCC 2 cut(s) 440, 448
Lsp1109I GCAGC 3 cut(s) 334, 592, 1090
LweI GCATC 4 cut(s) 13, 267, 373, 1096
MaeI CTAG 1 cut(s) 1082
MaeII ACGT 2 cut(s) 339, 1050
MaeIII GTNAC 4 cut(s) 493, 731, 833, 872
MalI GATC 4 cut(s) 234, 803, 933, 977
MbiI CCGCTC 1 cut(s) 1168
MboI GATC 4 cut(s) 232, 801, 931, 975
MboII GAAGA 7 cut(s) 25, 298, 364, 377, 565, 863, 926
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 3 cut(s) 118, 397, 1055
Mly113I GGCGCC 1 cut(s) 859
MlyI GAGTC 2 cut(s) 153, 572
MmeI TCCRAC 4 cut(s) 126, 519, 526, 1071
MroXI GAANNNNTTC 1 cut(s) 199
MseI TTAA 1 cut(s) 1101
MspCI CTTAAG 1 cut(s) 1100
MspI CCGG 5 cut(s) 76, 457, 531, 686, 862
MspR9I CCNGG 4 cut(s) 77, 170, 458, 532
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 164, 166
NarI GGCGCC 1 cut(s) 859
NciI CCSGG 3 cut(s) 77, 458, 532
NcoI CCATGG 1 cut(s) 727
NdeII GATC 4 cut(s) 232, 801, 931, 975
NheI GCTAGC 1 cut(s) 1081
NlaIII CATG 9 cut(s) 74, 220, 326, 563, 731, 880, 928, 1046, 1226
NlaIV GGNNCC 2 cut(s) 860, 1095
NmeAIII GCCGAG 2 cut(s) 1171, 1191
NmuCI GTSAC 3 cut(s) 493, 731, 833
NspI RCATGY 1 cut(s) 326
NspV TTCGAA 1 cut(s) 249
PagI TCATGA 2 cut(s) 216, 559
PaqCI CACCTGC 1 cut(s) 933
PauI GCGCGC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 2 cut(s) 398, 1143
PctI GAATGC 1 cut(s) 706
PdmI GAANNNNTTC 1 cut(s) 199
PfeI GAWTC 6 cut(s) 67, 98, 251, 556, 796, 880
PfoI TCCNGGA 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 685
PkrI GCNGC 4 cut(s) 321, 349, 582, 1080
PleI GAGTC 2 cut(s) 153, 571
PluTI GGCGCC 1 cut(s) 862
PpsI GAGTC 2 cut(s) 153, 571
Psp1406I AACGTT 2 cut(s) 339, 1050
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 2 cut(s) 88, 146
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 860, 1095
PteI GCGCGC 1 cut(s) 164
RsaI GTAC 2 cut(s) 599, 1041
RsaNI GTAC 2 cut(s) 598, 1040
SaqAI TTAA 1 cut(s) 1101
SatI GCNGC 4 cut(s) 320, 348, 581, 1079
Sau3AI GATC 4 cut(s) 232, 801, 931, 975
SchI GAGTC 2 cut(s) 153, 572
ScrFI CCNGG 4 cut(s) 77, 170, 458, 532
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 4 cut(s) 13, 267, 373, 1096
SfoI GGCGCC 1 cut(s) 860
SfuI TTCGAA 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 141, 1100
SmoI CTYRAG 2 cut(s) 141, 1100
Sse9I AATT 3 cut(s) 118, 397, 1055
SsiI CCGC 4 cut(s) 257, 319, 386, 1168
SspDI GGCGCC 1 cut(s) 858
SspMI CTAG 1 cut(s) 1082
StyD4I CCNGG 4 cut(s) 75, 168, 456, 530
StyI CCWWGG 1 cut(s) 727
TaiI ACGT 2 cut(s) 342, 1053
TaqI TCGA 4 cut(s) 177, 249, 401, 1164
TasI AATT 3 cut(s) 118, 397, 1055
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 6 cut(s) 67, 98, 251, 556, 796, 880
Tru1I TTAA 1 cut(s) 1101
Tru9I TTAA 1 cut(s) 1101
TscAI CASTG 1 cut(s) 740
TseFI GTSAC 3 cut(s) 493, 731, 833
TseI GCWGC 3 cut(s) 347, 580, 1078
Tsp45I GTSAC 3 cut(s) 493, 731, 833
TspDTI ATGAA 7 cut(s) 17, 26, 59, 195, 548, 689, 975
TspGWI ACGGA 2 cut(s) 257, 503
TspRI CASTG 1 cut(s) 740
Vha464I CTTAAG 1 cut(s) 1100
XapI RAATTY 2 cut(s) 118, 397
XceI RCATGY 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 199
XspI CTAG 1 cut(s) 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.