Rmu_sc0007525.1_g000005

Belongs to the Glu Leu Phe Val dehydrogenases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007525.1
OG0003656
Physical Location & Seq
Reverse (-)
13308 .. 18244
4937 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
atggagcggttgttagagcctgagagaatgattgtattccgagttccatgggtggatgatagaggtgagacacatgttaaccgaggatttcgggtacaatttaaccaggctttgggtccctgtaggggtggtttcagatttcatccttctatgagcttgagcgttgccaagtttcttgcatttgaacagactttaaggaatgctttgtcaccgtacaaaattggaggggcagcaggtggaagtgattttgatccaaagggaaaaagtgataatgaggtgatgcgtttttgtcaaagttttatgaatgagatctatcgctatttgggtattgacaaggaccttccttcagaggagatgggtgttggaactcgggaaatgggatatcttttcggacaatatagacgtctagctggtcattttcagggaagttttacggggccaaggatattttggtctggctctagccttcgcactgaagctactggatatggtctggttttctttgcacagctcttgcttgcagacctgaataaagattttaaaggaataagatgtgttgtaagcggctgtggaaagattgcattgcatgtcctggagaagcttattgcgtatggtgctcttcctgttacagtttctggttcaaatatgccgtgtacaccagaggctgttgatgttctaagaaaatctaatgttctaattgctcctgcagtggctgctggtgctggtggggtagttgctggagaatttgaattgaaccatgagtgtaattcagtgaactggtcaccgcaggactttgagtctaagctacaggaagcaataaaacaaacttaccagagatcccttaaagcagcgcttgactttggctactccaaagaaagtcctgagtatttggtacatggagcagtcatttctgcttttctgactcttgctcaagctatgacggatcagggttgtgtatag

Protein Analysis

319

Amino Acids

35.08

Weight (kDa)

7.0

Isoelectric Point (pI)

27.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ELFV_dehydrog_N PF02812 7 - 134 9.3e-45 Glu/Leu/Phe/Val dehydrogenase, dimerisation domain
ELFV_dehydrog PF00208 153 - 213 5.3e-11 Glutamate/Leucine/Phenylalanine/Valine dehydrogenase
ELFV_dehydrog PF00208 213 - 317 4.6e-20 Glutamate/Leucine/Phenylalanine/Valine dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
AatII GACGTC 1 cut(s) 406
Acc36I ACCTGC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 112
AccBSI CCGCTC 1 cut(s) 7
AciI CCGC 3 cut(s) 7, 564, 785
AclWI GGATC 3 cut(s) 245, 831, 951
AcsI RAATTY 1 cut(s) 743
AcuI CTGAAG 2 cut(s) 330, 495
AcyI GRCGYC 1 cut(s) 403
AfaI GTAC 4 cut(s) 96, 215, 655, 894
AfeI AGCGCT 1 cut(s) 852
AfiI CCNNNNNNNGG 2 cut(s) 112, 125
AflIII ACRYGT 1 cut(s) 73
AgsI TTSAA 4 cut(s) 185, 642, 749, 754
AhdI GACNNNNNGTC 1 cut(s) 796
AjnI CCWGG 2 cut(s) 105, 591
AluBI AGCT 7 cut(s) 156, 410, 479, 511, 601, 805, 935
AluI AGCT 7 cut(s) 156, 410, 479, 511, 601, 805, 935
Alw21I GWGCWC 1 cut(s) 619
Alw26I GTCTC 1 cut(s) 62
AlwI GGATC 3 cut(s) 245, 831, 951
AlwNI CAGNNNCTG 3 cut(s) 635, 665, 713
Ama87I CYCGRG 1 cut(s) 369
Aor51HI AGCGCT 1 cut(s) 852
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 3 cut(s) 230, 713, 848
ApoI RAATTY 1 cut(s) 743
AspLEI GCGC 1 cut(s) 853
AspS9I GGNCC 3 cut(s) 116, 337, 437
AsuHPI GGTGA 4 cut(s) 77, 201, 289, 774
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 2 cut(s) 116, 337
Bbv12I GWGCWC 1 cut(s) 619
BbvI GCAGC 3 cut(s) 242, 700, 860
BccI CCATC 1 cut(s) 349
BceAI ACGGC 1 cut(s) 634
BciT130I CCWGG 2 cut(s) 107, 593
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 2 cut(s) 407, 462
BfmI CTRYAG 3 cut(s) 121, 705, 806
BfoI RGCGCY 1 cut(s) 854
BfuAI ACCTGC 1 cut(s) 224
BglII AGATCT 1 cut(s) 309
BisI GCNGC 4 cut(s) 231, 565, 714, 849
BlsI GCNGC 4 cut(s) 232, 566, 715, 850
Bme1390I CCNGG 2 cut(s) 107, 593
Bme18I GGWCC 2 cut(s) 116, 337
BmeRI GACNNNNNGTC 1 cut(s) 796
BmeT110I CYCGRG 1 cut(s) 369
BmgT120I GGNCC 3 cut(s) 116, 337, 437
BmiI GGNNCC 3 cut(s) 117, 118, 438
BmrFI CCNGG 2 cut(s) 107, 593
BmsI GCATC 1 cut(s) 270
BpmI CTGGAG 2 cut(s) 614, 759
BpuEI CTTGAG 2 cut(s) 178, 915
BsaBI GATNNNNATC 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 403
BsaJI CCNNGG 3 cut(s) 47, 82, 440
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 125
Bse1I ACTGG 2 cut(s) 487, 782
Bse3DI GCAATG 1 cut(s) 581
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 2 cut(s) 107, 593
BseDI CCNNGG 3 cut(s) 47, 82, 440
BseGI GGATG 2 cut(s) 61, 142
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 112, 125
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 2 cut(s) 12, 873
BseNI ACTGG 2 cut(s) 487, 782
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 3 cut(s) 242, 700, 860
BshFI GGCC 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 619
BsiHKCI CYCGRG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 2 cut(s) 112, 125
BsmAI GTCTC 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 102
BsmI GAATGC 1 cut(s) 205
BsnI GGCC 1 cut(s) 439
BsoBI CYCGRG 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 619
Bsp1407I TGTACA 1 cut(s) 653
Bsp143I GATC 4 cut(s) 250, 309, 836, 943
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 3 cut(s) 7, 564, 785
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 2 cut(s) 13, 874
BspLI GGNNCC 3 cut(s) 117, 118, 438
BspMAI CTGCAG 1 cut(s) 709
BspMI ACCTGC 1 cut(s) 224
BspPI GGATC 3 cut(s) 245, 831, 951
BspQI GCTCTTC 1 cut(s) 624
BsrBI CCGCTC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 581
BsrGI TGTACA 1 cut(s) 653
BsrI ACTGG 2 cut(s) 487, 782
BssECI CCNNGG 3 cut(s) 47, 82, 440
BssMI GATC 4 cut(s) 250, 309, 836, 943
BssNI GRCGYC 1 cut(s) 403
BssT1I CCWWGG 2 cut(s) 47, 440
Bst2UI CCWGG 2 cut(s) 107, 593
Bst4CI ACNGT 2 cut(s) 213, 631
Bst6I CTCTTC 1 cut(s) 624
BstACI GRCGYC 1 cut(s) 403
BstAPI GCANNNNNTGC 1 cut(s) 713
BstAUI TGTACA 1 cut(s) 653
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 4 cut(s) 21, 677, 801, 882
BstDSI CCRYGG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 780
BstF5I GGATG 2 cut(s) 61, 142
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 1 cut(s) 853
BstKTI GATC 4 cut(s) 253, 312, 839, 946
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 4 cut(s) 250, 309, 836, 943
BstMWI GCNNNNNNNGC 3 cut(s) 614, 713, 719
BstNI CCWGG 2 cut(s) 107, 593
BstNSI RCATGY 2 cut(s) 77, 590
BstPI GGTNACC 1 cut(s) 780
BstSCI CCNGG 2 cut(s) 105, 591
BstSFI CTRYAG 3 cut(s) 121, 705, 806
BstV1I GCAGC 3 cut(s) 242, 700, 860
BstX2I RGATCY 2 cut(s) 309, 836
BstYI RGATCY 2 cut(s) 309, 836
BsuRI GGCC 1 cut(s) 439
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 2 cut(s) 61, 142
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 3 cut(s) 471, 714, 777
BveI ACCTGC 1 cut(s) 224
Cac8I GCNNGC 1 cut(s) 519
CaiI CAGNNNCTG 3 cut(s) 635, 665, 713
CfoI GCGC 1 cut(s) 853
Cfr13I GGNCC 3 cut(s) 116, 337, 437
Csp6I GTAC 4 cut(s) 95, 214, 654, 893
CviAII CATG 5 cut(s) 48, 74, 587, 758, 896
CviQI GTAC 4 cut(s) 95, 214, 654, 893
DdeI CTNAG 4 cut(s) 21, 677, 801, 882
DpnI GATC 4 cut(s) 252, 311, 838, 945
DpnII GATC 4 cut(s) 250, 309, 836, 943
DraI TTTAAA 1 cut(s) 541
DriI GACNNNNNGTC 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 624
Eam1105I GACNNNNNGTC 1 cut(s) 796
EarI CTCTTC 1 cut(s) 624
Eco130I CCWWGG 2 cut(s) 47, 440
Eco32I GATATC 1 cut(s) 383
Eco47I GGWCC 2 cut(s) 116, 337
Eco47III AGCGCT 1 cut(s) 852
Eco57I CTGAAG 2 cut(s) 330, 495
Eco88I CYCGRG 1 cut(s) 369
Eco91I GGTNACC 1 cut(s) 780
EcoO109I RGGNCCY 2 cut(s) 116, 337
EcoO65I GGTNACC 1 cut(s) 780
EcoRII CCWGG 2 cut(s) 105, 591
EcoRV GATATC 1 cut(s) 383
EcoT14I CCWWGG 2 cut(s) 47, 440
ErhI CCWWGG 2 cut(s) 47, 440
FaeI CATG 5 cut(s) 51, 77, 590, 761, 899
FalI AAGNNNNNCTT 4 cut(s) 187, 219, 837, 869
FaqI GGGAC 1 cut(s) 102
FatI CATG 5 cut(s) 47, 73, 586, 757, 895
Fnu4HI GCNGC 4 cut(s) 231, 565, 714, 849
FokI GGATG 2 cut(s) 68, 129
Fsp4HI GCNGC 4 cut(s) 231, 565, 714, 849
FspBI CTAG 2 cut(s) 407, 462
GlaI GCGC 1 cut(s) 852
GluI GCNGC 4 cut(s) 231, 565, 714, 849
GsuI CTGGAG 2 cut(s) 614, 759
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 1 cut(s) 439
HhaI GCGC 1 cut(s) 853
Hin1I GRCGYC 1 cut(s) 403
Hin1II CATG 5 cut(s) 51, 77, 590, 761, 899
Hin6I GCGC 1 cut(s) 851
HinP1I GCGC 1 cut(s) 851
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 2 cut(s) 797, 922
HpaI GTTAAC 1 cut(s) 79
HphI GGTGA 4 cut(s) 77, 201, 289, 774
Hpy166II GTNNAC 4 cut(s) 79, 654, 656, 775
Hpy188I TCNGA 5 cut(s) 41, 137, 349, 392, 921
Hpy188III TCNNGA 2 cut(s) 371, 881
Hpy8I GTNNAC 4 cut(s) 79, 654, 656, 775
HpyAV CCTTC 4 cut(s) 156, 350, 354, 476
HpyCH4III ACNGT 2 cut(s) 213, 631
HpyCH4IV ACGT 1 cut(s) 403
HpyCH4V TGCA 6 cut(s) 179, 506, 521, 581, 586, 707
HpyF10VI GCNNNNNNNGC 3 cut(s) 614, 713, 719
HpyF3I CTNAG 4 cut(s) 21, 677, 801, 882
HpySE526I ACGT 1 cut(s) 403
Hsp92I GRCGYC 1 cut(s) 403
Hsp92II CATG 5 cut(s) 51, 77, 590, 761, 899
HspAI GCGC 1 cut(s) 851
KflI GGGWCCC 1 cut(s) 116
KspAI GTTAAC 1 cut(s) 79
Kzo9I GATC 4 cut(s) 250, 309, 836, 943
LguI GCTCTTC 1 cut(s) 624
LmnI GCTCC 3 cut(s) 4, 706, 899
Lsp1109I GCAGC 3 cut(s) 242, 700, 860
LweI GCATC 1 cut(s) 270
MaeI CTAG 2 cut(s) 407, 462
MaeII ACGT 1 cut(s) 403
MaeIII GTNAC 3 cut(s) 207, 625, 780
MalI GATC 4 cut(s) 252, 311, 838, 945
MbiI CCGCTC 1 cut(s) 7
MboI GATC 4 cut(s) 250, 309, 836, 943
MboII GAAGA 1 cut(s) 611
MflI RGATCY 2 cut(s) 309, 836
MhlI GDGCHC 1 cut(s) 619
MluCI AATT 6 cut(s) 98, 219, 696, 743, 749, 766
MlyI GAGTC 2 cut(s) 806, 916
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 6 cut(s) 56, 77, 218, 268, 343, 655
MseI TTAA 5 cut(s) 78, 102, 194, 540, 843
MspR9I CCNGG 2 cut(s) 107, 593
Mva1269I GAATGC 1 cut(s) 205
MvaI CCWGG 2 cut(s) 107, 593
MwoI GCNNNNNNNGC 3 cut(s) 614, 713, 719
NcoI CCATGG 1 cut(s) 47
NdeII GATC 4 cut(s) 250, 309, 836, 943
NlaIII CATG 5 cut(s) 51, 77, 590, 761, 899
NlaIV GGNNCC 3 cut(s) 117, 118, 438
NmuCI GTSAC 2 cut(s) 207, 780
NspI RCATGY 2 cut(s) 77, 590
PaqCI CACCTGC 1 cut(s) 224
PciI ACATGT 1 cut(s) 73
PciSI GCTCTTC 1 cut(s) 624
PctI GAATGC 1 cut(s) 205
PflMI CCANNNNNTGG 1 cut(s) 112
PfoI TCCNGGA 1 cut(s) 591
PkrI GCNGC 4 cut(s) 232, 566, 715, 850
PleI GAGTC 2 cut(s) 805, 916
PpsI GAGTC 2 cut(s) 805, 916
PpuMI RGGWCCY 2 cut(s) 116, 337
PscI ACATGT 1 cut(s) 73
Psp5II RGGWCCY 2 cut(s) 116, 337
Psp6I CCWGG 2 cut(s) 105, 591
PspEI GGTNACC 1 cut(s) 780
PspGI CCWGG 2 cut(s) 105, 591
PspN4I GGNNCC 3 cut(s) 117, 118, 438
PspPI GGNCC 3 cut(s) 116, 337, 437
PspPPI RGGWCCY 2 cut(s) 116, 337
PsrI GAACNNNNNNTAC 2 cut(s) 27, 59
PstI CTGCAG 1 cut(s) 709
PstNI CAGNNNCTG 3 cut(s) 635, 665, 713
PsuI RGATCY 2 cut(s) 309, 836
RsaI GTAC 4 cut(s) 96, 215, 655, 894
RsaNI GTAC 4 cut(s) 95, 214, 654, 893
SapI GCTCTTC 1 cut(s) 624
SaqAI TTAA 5 cut(s) 78, 102, 194, 540, 843
SatI GCNGC 4 cut(s) 231, 565, 714, 849
Sau3AI GATC 4 cut(s) 250, 309, 836, 943
Sau96I GGNCC 3 cut(s) 116, 337, 437
SchI GAGTC 2 cut(s) 806, 916
ScrFI CCNGG 2 cut(s) 107, 593
SduI GDGCHC 1 cut(s) 619
SfaNI GCATC 1 cut(s) 270
SfcI CTRYAG 3 cut(s) 121, 705, 806
SinI GGWCC 2 cut(s) 116, 337
SmlI CTYRAG 2 cut(s) 157, 930
SmoI CTYRAG 2 cut(s) 157, 930
Sse9I AATT 6 cut(s) 98, 219, 696, 743, 749, 766
SsiI CCGC 3 cut(s) 7, 564, 785
SspMI CTAG 2 cut(s) 407, 462
StyD4I CCNGG 2 cut(s) 105, 591
StyI CCWWGG 2 cut(s) 47, 440
TaaI ACNGT 2 cut(s) 213, 631
TaiI ACGT 1 cut(s) 406
TasI AATT 6 cut(s) 98, 219, 696, 743, 749, 766
TatI WGTACW 1 cut(s) 653
TauI GCSGC 1 cut(s) 567
Tru1I TTAA 5 cut(s) 78, 102, 194, 540, 843
Tru9I TTAA 5 cut(s) 78, 102, 194, 540, 843
TscAI CASTG 3 cut(s) 478, 714, 777
TseFI GTSAC 2 cut(s) 207, 780
TseI GCWGC 3 cut(s) 230, 713, 848
Tsp45I GTSAC 2 cut(s) 207, 780
TspDTI ATGAA 2 cut(s) 131, 317
TspGWI ACGGA 1 cut(s) 956
TspRI CASTG 3 cut(s) 478, 714, 777
Van91I CCANNNNNTGG 1 cut(s) 112
VpaK11BI GGWCC 2 cut(s) 116, 337
XapI RAATTY 1 cut(s) 743
XceI RCATGY 2 cut(s) 77, 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 447
XspI CTAG 2 cut(s) 407, 462
ZraI GACGTC 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.