Rmu_sc0008322.1_g000019

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008322.1
OG0019829
Physical Location & Seq
Forward (+)
109687 .. 112160
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008322.1_g000019.1.cds

Sequence Viewer

Length: 645 bp
atggttagagcttctagagttgggtcatcaacacagggagcagggaatacgggagggttggctggcggccgtattcccttatctacgatctggtcaggaggttgtgcctatgccctcgggatattaataggtattggcaagtcaagttggaaggggaaagatgtacatgcaaaagactctctgatacctaattttgtaagtgttgaatttgtttacaatgagatctgtttggcatggggagttaaaactgatatgcgaaagcttgaaggcaccatgtccaccatcaaaagtgcactctatgatgctgaagagaagcaatggagtaacaagagggcacaaagttggctgcaacaacttaaacatgtgttccgcgaagctgaagatgtgttggatgagtttgagtgcgaagctttgcagagccaagacactgtgagaaatattgagggtaggggtcatatattgcctgctttgaggttgagttatactcagttgccttcatacatgaaaccatggcttgcttattatgcatatcttccaaagaaaggaattaaatggagttctctgcaattgatctattgttggatggctcatggaatccttcaatctcatgataagtatctggagatggaagatgttggggagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

214

Amino Acids

24.01

Weight (kDa)

7.66

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019829)

Species Orthologous Gene IDs
pyrus_communis pycom14g06190
rosa_multiflora Rmu_co8262551.1_g000001 Rmu_sc0008322.1_g000019
rosa_roxburghii Rroxscaffold_2G00152090
rosa_rugosa Rorug01G0488700
rosa_samantha Rh2AG044800 Rh3BG279700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AccII CGCG 1 cut(s) 372
AciI CCGC 2 cut(s) 66, 370
AcoI YGGCCR 1 cut(s) 67
AcsI RAATTY 1 cut(s) 206
AcuI CTGAAG 2 cut(s) 327, 399
AfaI GTAC 1 cut(s) 165
AfiI CCNNNNNNNGG 1 cut(s) 542
AflIII ACRYGT 1 cut(s) 361
AgsI TTSAA 3 cut(s) 206, 266, 602
AluBI AGCT 4 cut(s) 11, 262, 377, 410
AluI AGCT 4 cut(s) 11, 262, 377, 410
Alw21I GWGCWC 1 cut(s) 295
Alw44I GTGCAC 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 1 cut(s) 67
ApaLI GTGCAC 1 cut(s) 291
ApeKI GCWGC 1 cut(s) 346
ApoI RAATTY 1 cut(s) 206
AseI ATTAAT 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 116
BaeGI GKGCMC 2 cut(s) 295, 337
BanI GGYRCC 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 1 cut(s) 333
BccI CCATC 3 cut(s) 290, 577, 619
BceAI ACGGC 1 cut(s) 54
BfaI CTAG 1 cut(s) 15
BglII AGATCT 1 cut(s) 222
BisI GCNGC 2 cut(s) 67, 347
BlsI GCNGC 2 cut(s) 68, 348
BmeT110I CYCGRG 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 271
BmsI GCATC 1 cut(s) 292
BplI GAGNNNNNCTC 2 cut(s) 469, 501
BpmI CTGGAG 1 cut(s) 641
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 2 cut(s) 115, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 542
Bse3DI GCAATG 1 cut(s) 323
Bse8I GATNNNNATC 1 cut(s) 615
BseDI CCNNGG 2 cut(s) 115, 509
BseGI GGATG 2 cut(s) 397, 588
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 1 cut(s) 542
BseMI GCAATG 1 cut(s) 323
BseMII CTCAG 1 cut(s) 500
BseSI GKGCMC 2 cut(s) 295, 337
BseX3I CGGCCG 1 cut(s) 67
BseXI GCAGC 1 cut(s) 333
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 1 cut(s) 69
BshNI GGYRCC 1 cut(s) 269
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 295
BsiHKCI CYCGRG 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 542
BsnI GGCC 1 cut(s) 69
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 295, 337
Bsp1407I TGTACA 1 cut(s) 163
Bsp143I GATC 3 cut(s) 87, 222, 570
Bsp19I CCATGG 1 cut(s) 509
BspACI CCGC 2 cut(s) 66, 370
BspANI GGCC 1 cut(s) 69
BspCNI CTCAG 1 cut(s) 499
BspFNI CGCG 1 cut(s) 372
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 1 cut(s) 271
BspT107I GGYRCC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 163
BssECI CCNNGG 2 cut(s) 115, 509
BssMI GATC 3 cut(s) 87, 222, 570
BssT1I CCWWGG 1 cut(s) 509
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 303
BstAUI TGTACA 1 cut(s) 163
BstC8I GCNNGC 3 cut(s) 64, 465, 516
BstDEI CTNAG 1 cut(s) 486
BstDSI CCRYGG 1 cut(s) 509
BstF5I GGATG 2 cut(s) 397, 588
BstFNI CGCG 1 cut(s) 372
BstKTI GATC 3 cut(s) 90, 225, 573
BstMBI GATC 3 cut(s) 87, 222, 570
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 1 cut(s) 524
BstNSI RCATGY 2 cut(s) 170, 365
BstSLI GKGCMC 2 cut(s) 295, 337
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BstZI CGGCCG 1 cut(s) 67
BsuRI GGCC 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 509
BtsCI GGATG 2 cut(s) 397, 588
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 3 cut(s) 64, 465, 516
CciI TCATGA 1 cut(s) 607
Csp6I GTAC 1 cut(s) 164
CviAII CATG 8 cut(s) 167, 234, 274, 362, 502, 510, 590, 608
CviQI GTAC 1 cut(s) 164
DdeI CTNAG 1 cut(s) 486
DpnI GATC 3 cut(s) 89, 224, 572
DpnII GATC 3 cut(s) 87, 222, 570
EaeI YGGCCR 1 cut(s) 67
EagI CGGCCG 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
EclXI CGGCCG 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 509
Eco52I CGGCCG 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 327, 399
Eco88I CYCGRG 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 509
EcoT22I ATGCAT 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 509
FaeI CATG 8 cut(s) 170, 237, 277, 365, 505, 513, 593, 611
FatI CATG 8 cut(s) 166, 233, 273, 361, 501, 509, 589, 607
Fnu4HI GCNGC 2 cut(s) 67, 347
FokI GGATG 2 cut(s) 404, 595
Fsp4HI GCNGC 2 cut(s) 67, 347
FspBI CTAG 1 cut(s) 15
GluI GCNGC 2 cut(s) 67, 347
GsuI CTGGAG 1 cut(s) 641
HaeIII GGCC 1 cut(s) 69
Hin1II CATG 8 cut(s) 170, 237, 277, 365, 505, 513, 593, 611
HindIII AAGCTT 2 cut(s) 260, 408
HinfI GANTC 2 cut(s) 176, 594
Hpy166II GTNNAC 3 cut(s) 214, 279, 293
Hpy188I TCNGA 1 cut(s) 183
Hpy188III TCNNGA 5 cut(s) 15, 96, 118, 608, 620
Hpy8I GTNNAC 3 cut(s) 214, 279, 293
HpyAV CCTTC 4 cut(s) 145, 260, 504, 608
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4V TGCA 6 cut(s) 170, 293, 349, 415, 527, 565
HpyF10VI GCNNNNNNNGC 1 cut(s) 524
HpyF3I CTNAG 1 cut(s) 486
Hsp92II CATG 8 cut(s) 170, 237, 277, 365, 505, 513, 593, 611
Kzo9I GATC 3 cut(s) 87, 222, 570
LmnI GCTCC 1 cut(s) 38
LpnPI CCDG 7 cut(s) 20, 27, 48, 76, 81, 477, 605
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 1 cut(s) 292
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 323
MalI GATC 3 cut(s) 89, 224, 572
MboI GATC 3 cut(s) 87, 222, 570
MboII GAAGA 4 cut(s) 320, 392, 524, 641
MfeI CAATTG 1 cut(s) 566
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 2 cut(s) 295, 337
MluCI AATT 4 cut(s) 190, 206, 546, 566
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 3 cut(s) 128, 369, 560
MnlI CCTC 6 cut(s) 47, 92, 125, 324, 436, 465
Mph1103I ATGCAT 1 cut(s) 529
MseI TTAA 4 cut(s) 125, 243, 357, 549
MunI CAATTG 1 cut(s) 566
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 524
NcoI CCATGG 1 cut(s) 509
NdeII GATC 3 cut(s) 87, 222, 570
NlaIII CATG 8 cut(s) 170, 237, 277, 365, 505, 513, 593, 611
NlaIV GGNNCC 1 cut(s) 271
NsiI ATGCAT 1 cut(s) 529
NspI RCATGY 2 cut(s) 170, 365
PagI TCATGA 1 cut(s) 607
PciI ACATGT 1 cut(s) 361
PfeI GAWTC 1 cut(s) 594
PkrI GCNGC 2 cut(s) 68, 348
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PscI ACATGT 1 cut(s) 361
PshBI ATTAAT 1 cut(s) 125
PspN4I GGNNCC 1 cut(s) 271
PsuI RGATCY 1 cut(s) 222
RsaI GTAC 1 cut(s) 165
RsaNI GTAC 1 cut(s) 164
SaqAI TTAA 4 cut(s) 125, 243, 357, 549
SatI GCNGC 2 cut(s) 67, 347
Sau3AI GATC 3 cut(s) 87, 222, 570
SchI GAGTC 1 cut(s) 170
SduI GDGCHC 2 cut(s) 295, 337
SetI ASST 8 cut(s) 13, 103, 133, 190, 264, 379, 412, 476
SfaNI GCATC 1 cut(s) 292
Sse9I AATT 4 cut(s) 190, 206, 546, 566
SsiI CCGC 2 cut(s) 66, 370
SspI AATATT 1 cut(s) 439
SspMI CTAG 1 cut(s) 15
StyI CCWWGG 1 cut(s) 509
TaaI ACNGT 1 cut(s) 430
TasI AATT 4 cut(s) 190, 206, 546, 566
TatI WGTACW 1 cut(s) 163
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 1 cut(s) 594
Tru1I TTAA 4 cut(s) 125, 243, 357, 549
Tru9I TTAA 4 cut(s) 125, 243, 357, 549
TscAI CASTG 1 cut(s) 433
TseI GCWGC 1 cut(s) 346
TspDTI ATGAA 2 cut(s) 486, 518
TspRI CASTG 1 cut(s) 433
VneI GTGCAC 1 cut(s) 291
VspI ATTAAT 1 cut(s) 125
XapI RAATTY 1 cut(s) 206
XbaI TCTAGA 1 cut(s) 14
XceI RCATGY 2 cut(s) 170, 365
XspI CTAG 1 cut(s) 15
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.