Rmu_sc0010071.1_g000004

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010071.1
OG0018923
Physical Location & Seq
Forward (+)
11434 .. 17530
6097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010071.1_g000004.1.cds

Sequence Viewer

Length: 1233 bp
atggaggccaggggtcgaacggaccaatctgcagttgggtggagtcaccggcgacgaggttgcagtgctgcccagaaccttgcagttgcaggtaaggtcacggcccagaatccttcaacctcgatctccttcgacctcgatcgctacctaaaagaggtctgggttgcttccggcctccgtcagtcaagccccgcgtcgccgcctaaaattctgcaaaagcatcgacttctgctccaaaactcgtctgcggcaatggtgacgctcgacttgcaggagggagaccggaggcatcccggacggttctgggcagcgttcgaggtcggaggagtgagctgcaacctagtcgccggcgactccaccgactgcagacctctccgtccgacccctgcagtctgtccgcgaagccctgccgctccgtcgaaccccgagcggagctgcagcgacgacgttcgtactttagcaacagtgtggacttcctcttcagattttcgtatatatatacagcaaggttccaaagaggacgtccttgctatacataatctgcgaataggggacgggaagagaacgaacaatgcaaatctcggttcttgggttcatctgagaagaacacaaatctggagacagatagcgagagcgaagagagcaaaggaagaagggaaagaagaagacattcgggagaagacagaaaagagaacggcagaagggtgccggaatcataccccgcacacttctgaagtcaaggacagccagggaacaactcgtacttttgatctcgtcgatggtggggttgccgcaaacaaccctgtaagtattttaggcactgccaagttcgaagggaagtgcagtgcaaccacggcctcaaaatggggtttgatcaattggatgtttgacaatgggagcacacctatgattgatatctttacagatgcaagttcggatatggttgacattcatgtctccactctctttcagtccctcaatgccaaagacaactacctacgtattcaggatgacagtttgatcggtgaggagtcatcggtggatattgcaacggagaaaaatcttaagaggcttgtggagatcgggaatgggctgttgagaaagccggtgtcaagggtaaatttggataacgggaggtatgagaaatctgaaggagaaggtacctatgaagaagcactggtcgattttgccaaacggctcgtggaggggaagaagcacaggcaatacaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

410

Amino Acids

45.39

Weight (kDa)

8.98

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 375, 953
AatII GACGTC 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 80
Acc65I GGTACC 1 cut(s) 1160
AccB1I GGYRCC 2 cut(s) 705, 1160
AccBSI CCGCTC 2 cut(s) 413, 430
AccII CGCG 2 cut(s) 194, 400
AciI CCGC 8 cut(s) 192, 200, 248, 398, 411, 430, 722, 792
AcsI RAATTY 2 cut(s) 207, 1120
AcuI CTGAAG 3 cut(s) 465, 753, 1170
AcyI GRCGYC 1 cut(s) 522
AfaI GTAC 3 cut(s) 454, 763, 1162
AfiI CCNNNNNNNGG 2 cut(s) 154, 864
AflII CTTAAG 1 cut(s) 1064
AgsI TTSAA 1 cut(s) 117
AjnI CCWGG 2 cut(s) 8, 747
AluBI AGCT 2 cut(s) 333, 435
AluI AGCT 2 cut(s) 333, 435
Alw21I GWGCWC 1 cut(s) 902
Alw26I GTCTC 3 cut(s) 273, 613, 961
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 4 cut(s) 6, 102, 172, 855
ApeKI GCWGC 5 cut(s) 68, 308, 333, 435, 438
ApoI RAATTY 2 cut(s) 207, 1120
ArsI GACNNNNNNTTYG 2 cut(s) 507, 539
Asp700I GAANNNNTTC 1 cut(s) 669
Asp718I GGTACC 1 cut(s) 1160
AspS9I GGNCC 2 cut(s) 22, 103
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 3 cut(s) 38, 268, 1037
AsuII TTCGAA 1 cut(s) 831
AvaI CYCGRG 1 cut(s) 425
AvaII GGWCC 1 cut(s) 22
BanI GGYRCC 2 cut(s) 705, 1160
BauI CACGAG 1 cut(s) 1199
BbsI GAAGAC 2 cut(s) 672, 686
Bbv12I GWGCWC 1 cut(s) 902
BbvI GCAGC 5 cut(s) 55, 320, 320, 422, 450
BccI CCATC 1 cut(s) 773
BceAI ACGGC 4 cut(s) 117, 711, 870, 1211
BcgI CGANNNNNNTGC 6 cut(s) 42, 76, 203, 237, 325, 359
BciT130I CCWGG 2 cut(s) 10, 749
BclI TGATCA 1 cut(s) 873
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 3 cut(s) 273, 613, 961
BfaI CTAG 1 cut(s) 341
BfmI CTRYAG 4 cut(s) 30, 364, 387, 436
BfrI CTTAAG 1 cut(s) 1064
BfuAI ACCTGC 1 cut(s) 80
BisI GCNGC 9 cut(s) 69, 200, 249, 309, 334, 411, 436, 439, 792
BlsI GCNGC 9 cut(s) 70, 201, 250, 310, 335, 412, 437, 440, 793
Bme1390I CCNGG 3 cut(s) 10, 294, 749
Bme18I GGWCC 1 cut(s) 22
BmeT110I CYCGRG 1 cut(s) 425
BmgT120I GGNCC 2 cut(s) 22, 103
BmiI GGNNCC 3 cut(s) 511, 707, 1162
BmrFI CCNGG 3 cut(s) 10, 294, 749
BmsI GCATC 3 cut(s) 229, 298, 916
BpiI GAAGAC 2 cut(s) 672, 686
BpmI CTGGAG 1 cut(s) 637
Bpu14I TTCGAA 1 cut(s) 831
BpuMI CCSGG 1 cut(s) 294
BsaAI YACGTR 1 cut(s) 1001
BsaBI GATNNNNATC 1 cut(s) 914
BsaHI GRCGYC 1 cut(s) 522
BsaI GGTCTC 1 cut(s) 273
BsaJI CCNNGG 3 cut(s) 9, 748, 852
BsaWI WCCGGW 1 cut(s) 282
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 864
Bse118I RCCGGY 3 cut(s) 48, 347, 1105
Bse1I ACTGG 1 cut(s) 1182
Bse3DI GCAATG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 914
BseBI CCWGG 2 cut(s) 10, 749
BseDI CCNNGG 3 cut(s) 9, 748, 852
BseGI GGATG 3 cut(s) 289, 888, 1015
BseJI GATNNNNATC 1 cut(s) 914
BseLI CCNNNNNNNGG 2 cut(s) 154, 864
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 590
BseNI ACTGG 1 cut(s) 1182
BseRI GAGGAG 2 cut(s) 339, 1043
BseXI GCAGC 5 cut(s) 55, 320, 320, 422, 450
BsgI GTGCAG 1 cut(s) 862
Bsh1236I CGCG 2 cut(s) 194, 400
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 4 cut(s) 8, 104, 174, 857
BshNI GGYRCC 2 cut(s) 705, 1160
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 902
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 7 cut(s) 49, 171, 283, 294, 348, 709, 1106
BslFI GGGAC 2 cut(s) 566, 958
BslI CCNNNNNNNGG 2 cut(s) 154, 864
BsmAI GTCTC 3 cut(s) 273, 613, 961
BsmFI GGGAC 2 cut(s) 566, 958
BsnI GGCC 4 cut(s) 8, 104, 174, 857
Bso31I GGTCTC 1 cut(s) 273
BsoBI CYCGRG 1 cut(s) 425
Bsp119I TTCGAA 1 cut(s) 831
Bsp1286I GDGCHC 1 cut(s) 902
Bsp143I GATC 6 cut(s) 123, 139, 769, 873, 1020, 1080
BspACI CCGC 8 cut(s) 192, 200, 248, 398, 411, 430, 722, 792
BspANI GGCC 4 cut(s) 8, 104, 174, 857
BspCNI CTCAG 1 cut(s) 591
BspFNI CGCG 2 cut(s) 194, 400
BspLI GGNNCC 3 cut(s) 511, 707, 1162
BspMAI CTGCAG 4 cut(s) 34, 368, 391, 440
BspMI ACCTGC 1 cut(s) 80
BspT104I TTCGAA 1 cut(s) 831
BspT107I GGYRCC 2 cut(s) 705, 1160
BspTI CTTAAG 1 cut(s) 1064
BspTNI GGTCTC 1 cut(s) 273
BsrBI CCGCTC 2 cut(s) 413, 430
BsrDI GCAATG 1 cut(s) 258
BsrFI RCCGGY 3 cut(s) 48, 347, 1105
BsrI ACTGG 1 cut(s) 1182
BssAI RCCGGY 3 cut(s) 48, 347, 1105
BssECI CCNNGG 3 cut(s) 9, 748, 852
BssMI GATC 6 cut(s) 123, 139, 769, 873, 1020, 1080
BssNI GRCGYC 1 cut(s) 522
BssSI CACGAG 1 cut(s) 1199
Bst2BI CACGAG 1 cut(s) 1199
Bst2UI CCWGG 2 cut(s) 10, 749
Bst4CI ACNGT 3 cut(s) 300, 466, 1016
Bst6I CTCTTC 3 cut(s) 484, 554, 632
BstACI GRCGYC 1 cut(s) 522
BstAFI CTTAAG 1 cut(s) 1064
BstBAI YACGTR 1 cut(s) 1001
BstBI TTCGAA 1 cut(s) 831
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 1 cut(s) 599
BstDSI CCRYGG 1 cut(s) 852
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 3 cut(s) 289, 888, 1015
BstFNI CGCG 2 cut(s) 194, 400
BstKTI GATC 6 cut(s) 126, 142, 772, 876, 1023, 1083
BstMAI GTCTC 3 cut(s) 273, 613, 961
BstMBI GATC 6 cut(s) 123, 139, 769, 873, 1020, 1080
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 3 cut(s) 268, 641, 854
BstNI CCWGG 2 cut(s) 10, 749
BstSCI CCNGG 3 cut(s) 8, 292, 747
BstSFI CTRYAG 4 cut(s) 30, 364, 387, 436
BstSNI TACGTA 1 cut(s) 1001
BstUI CGCG 2 cut(s) 194, 400
BstV1I GCAGC 5 cut(s) 55, 320, 320, 422, 450
BstV2I GAAGAC 2 cut(s) 672, 686
BsuRI GGCC 4 cut(s) 8, 104, 174, 857
BtgI CCRYGG 1 cut(s) 852
BtsCI GGATG 3 cut(s) 289, 888, 1015
BtsI GCAGTG 3 cut(s) 70, 819, 850
BtsIMutI CAGTG 5 cut(s) 70, 471, 819, 850, 1175
BveI ACCTGC 1 cut(s) 80
Cac8I GCNNGC 1 cut(s) 349
Cfr10I RCCGGY 3 cut(s) 48, 347, 1105
Cfr13I GGNCC 2 cut(s) 22, 103
CseI GACGC 2 cut(s) 183, 268
Csp6I GTAC 3 cut(s) 453, 762, 1161
CviAII CATG 1 cut(s) 953
CviQI GTAC 3 cut(s) 453, 762, 1161
DdeI CTNAG 1 cut(s) 599
DpnI GATC 6 cut(s) 125, 141, 771, 875, 1022, 1082
DpnII GATC 6 cut(s) 123, 139, 769, 873, 1020, 1080
DrdI GACNNNNNNGTC 2 cut(s) 375, 953
DseDI GACNNNNNNGTC 2 cut(s) 375, 953
Eam1104I CTCTTC 3 cut(s) 484, 554, 632
EarI CTCTTC 3 cut(s) 484, 554, 632
Eco105I TACGTA 1 cut(s) 1001
Eco31I GGTCTC 1 cut(s) 273
Eco32I GATATC 1 cut(s) 916
Eco47I GGWCC 1 cut(s) 22
Eco57I CTGAAG 3 cut(s) 465, 753, 1170
Eco88I CYCGRG 1 cut(s) 425
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoRII CCWGG 2 cut(s) 8, 747
EcoRV GATATC 1 cut(s) 916
FaeI CATG 1 cut(s) 956
FaqI GGGAC 2 cut(s) 566, 958
FatI CATG 1 cut(s) 952
FauI CCCGC 2 cut(s) 199, 729
FbaI TGATCA 1 cut(s) 873
Fnu4HI GCNGC 9 cut(s) 69, 200, 249, 309, 334, 411, 436, 439, 792
FokI GGATG 3 cut(s) 276, 895, 1022
Fsp4HI GCNGC 9 cut(s) 69, 200, 249, 309, 334, 411, 436, 439, 792
FspBI CTAG 1 cut(s) 341
GluI GCNGC 9 cut(s) 69, 200, 249, 309, 334, 411, 436, 439, 792
GsuI CTGGAG 1 cut(s) 637
HaeIII GGCC 4 cut(s) 8, 104, 174, 857
HapII CCGG 7 cut(s) 49, 171, 283, 294, 348, 709, 1106
HgaI GACGC 2 cut(s) 183, 268
Hin1I GRCGYC 1 cut(s) 522
Hin1II CATG 1 cut(s) 956
HincII GTYRAC 1 cut(s) 946
HindII GTYRAC 1 cut(s) 946
HinfI GANTC 5 cut(s) 43, 109, 353, 712, 1031
HpaII CCGG 7 cut(s) 49, 171, 283, 294, 348, 709, 1106
HphI GGTGA 3 cut(s) 38, 268, 1037
Hpy166II GTNNAC 2 cut(s) 471, 946
Hpy188I TCNGA 7 cut(s) 323, 381, 484, 600, 733, 937, 1150
Hpy188III TCNNGA 4 cut(s) 616, 674, 1007, 1084
Hpy8I GTNNAC 2 cut(s) 471, 946
Hpy99I CGWCG 6 cut(s) 57, 199, 421, 446, 449, 779
HpyAV CCTTC 7 cut(s) 123, 139, 647, 695, 827, 1145, 1151
HpyCH4III ACNGT 3 cut(s) 300, 466, 1016
HpyCH4IV ACGT 3 cut(s) 447, 522, 1000
HpyF10VI GCNNNNNNNGC 3 cut(s) 268, 641, 854
HpyF3I CTNAG 1 cut(s) 599
HpySE526I ACGT 3 cut(s) 447, 522, 1000
Hsp92I GRCGYC 1 cut(s) 522
Hsp92II CATG 1 cut(s) 956
KpnI GGTACC 1 cut(s) 1164
KroI GCCGGC 1 cut(s) 347
KroNI GCCGGC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 873
Kzo9I GATC 6 cut(s) 123, 139, 769, 873, 1020, 1080
LmnI GCTCC 4 cut(s) 237, 418, 432, 897
Lsp1109I GCAGC 5 cut(s) 55, 320, 320, 422, 450
LweI GCATC 3 cut(s) 229, 298, 916
MaeI CTAG 1 cut(s) 341
MaeII ACGT 3 cut(s) 447, 522, 1000
MaeIII GTNAC 3 cut(s) 44, 97, 256
MalI GATC 6 cut(s) 125, 141, 771, 875, 1022, 1082
MbiI CCGCTC 2 cut(s) 413, 430
MboI GATC 6 cut(s) 123, 139, 769, 873, 1020, 1080
MfeI CAATTG 1 cut(s) 877
MhlI GDGCHC 1 cut(s) 902
MluCI AATT 3 cut(s) 207, 877, 1120
MlyI GAGTC 3 cut(s) 52, 347, 1040
MmeI TCCRAC 2 cut(s) 301, 404
MreI CGCCGGCG 1 cut(s) 347
MroNI GCCGGC 1 cut(s) 347
MroXI GAANNNNTTC 1 cut(s) 669
MseI TTAA 1 cut(s) 1065
MslI CAYNNNNRTG 1 cut(s) 905
MspCI CTTAAG 1 cut(s) 1064
MspI CCGG 7 cut(s) 49, 171, 283, 294, 348, 709, 1106
MspR9I CCNGG 3 cut(s) 10, 294, 749
MunI CAATTG 1 cut(s) 877
MvaI CCWGG 2 cut(s) 10, 749
MvnI CGCG 2 cut(s) 194, 400
MwoI GCNNNNNNNGC 3 cut(s) 268, 641, 854
NaeI GCCGGC 1 cut(s) 349
NciI CCSGG 1 cut(s) 294
NdeII GATC 6 cut(s) 123, 139, 769, 873, 1020, 1080
NgoMIV GCCGGC 1 cut(s) 347
NlaIII CATG 1 cut(s) 956
NlaIV GGNNCC 3 cut(s) 511, 707, 1162
NmuCI GTSAC 3 cut(s) 44, 97, 256
NspV TTCGAA 1 cut(s) 831
PdiI GCCGGC 1 cut(s) 349
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 2 cut(s) 109, 712
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 9 cut(s) 70, 201, 250, 310, 335, 412, 437, 440, 793
Ple19I CGATCG 1 cut(s) 142
PleI GAGTC 3 cut(s) 51, 347, 1039
PpsI GAGTC 3 cut(s) 51, 347, 1039
Ppu21I YACGTR 1 cut(s) 1001
Psp6I CCWGG 2 cut(s) 8, 747
PspGI CCWGG 2 cut(s) 8, 747
PspN4I GGNNCC 3 cut(s) 511, 707, 1162
PspPI GGNCC 2 cut(s) 22, 103
PsrI GAACNNNNNNTAC 2 cut(s) 745, 777
PstI CTGCAG 4 cut(s) 34, 368, 391, 440
PvuI CGATCG 1 cut(s) 142
RsaI GTAC 3 cut(s) 454, 763, 1162
RsaNI GTAC 3 cut(s) 453, 762, 1161
RseI CAYNNNNRTG 1 cut(s) 905
SaqAI TTAA 1 cut(s) 1065
SatI GCNGC 9 cut(s) 69, 200, 249, 309, 334, 411, 436, 439, 792
Sau3AI GATC 6 cut(s) 123, 139, 769, 873, 1020, 1080
Sau96I GGNCC 2 cut(s) 22, 103
SchI GAGTC 3 cut(s) 52, 347, 1040
ScrFI CCNGG 3 cut(s) 10, 294, 749
SduI GDGCHC 1 cut(s) 902
SfaNI GCATC 3 cut(s) 229, 298, 916
SfcI CTRYAG 4 cut(s) 30, 364, 387, 436
SfuI TTCGAA 1 cut(s) 831
SgrAI CRCCGGYG 2 cut(s) 48, 347
SinI GGWCC 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 905
SmlI CTYRAG 1 cut(s) 1064
SmoI CTYRAG 1 cut(s) 1064
SnaBI TACGTA 1 cut(s) 1001
Sse9I AATT 3 cut(s) 207, 877, 1120
SsiI CCGC 8 cut(s) 192, 200, 248, 398, 411, 430, 722, 792
SspMI CTAG 1 cut(s) 341
StyD4I CCNGG 3 cut(s) 8, 292, 747
TaaI ACNGT 3 cut(s) 300, 466, 1016
TaiI ACGT 3 cut(s) 450, 525, 1003
TasI AATT 3 cut(s) 207, 877, 1120
TauI GCSGC 4 cut(s) 202, 251, 413, 794
TfiI GAWTC 2 cut(s) 109, 712
Tru1I TTAA 1 cut(s) 1065
Tru9I TTAA 1 cut(s) 1065
TscAI CASTG 5 cut(s) 70, 471, 826, 850, 1182
TseFI GTSAC 3 cut(s) 44, 97, 256
TseI GCWGC 5 cut(s) 68, 308, 333, 435, 438
Tsp45I GTSAC 3 cut(s) 44, 97, 256
TspDTI ATGAA 3 cut(s) 584, 941, 1182
TspGWI ACGGA 5 cut(s) 35, 167, 365, 405, 1067
TspRI CASTG 5 cut(s) 70, 471, 826, 850, 1182
Vha464I CTTAAG 1 cut(s) 1064
VpaK11BI GGWCC 1 cut(s) 22
XagI CCTNNNNNAGG 1 cut(s) 152
XapI RAATTY 2 cut(s) 207, 1120
XcmI CCANNNNNNNNNTGG 2 cut(s) 32, 1198
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 1 cut(s) 341
ZraI GACGTC 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.