Rmu_sc0029770.1_g000001

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0029770.1
OG0018923
Physical Location & Seq
Reverse (-)
1 .. 996
996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0029770.1_g000001.1.cds

Sequence Viewer

Length: 567 bp
atgcaggatgagggcactagtgagcaatttcaactcctatggaccgaaatgcttaattgtttaagacgaagtcagcctccaagagtctttgattatgcatttctcaaactccaggcctccatccttgagaaaactattgatcacgcaaaactgtcaatttcagagccaaccatcaccttttggaactccacatatggtgaacaaatcaacttggattacccccagaccatgctccgtgtcctagacaagctgtggagacggggaagaataaacctccacaggagaagacctcttcgaagatgtcaatctaaatcggaagccattactgctccactaggatttacagtcaacacaacacacaatagacactctgctaaggtcgcaacggcgcgacggcgctgcgccgcttgctggacggaggccaggggtctgacggaccagtctgcaatcgggtggagtcgacggagacgaggttgcaccgttgcaggtcaggttgctacccagaacctgcaacctcgatcactgcccaaaacagtctggggttggattgtagcagacacggcattg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

189

Amino Acids

21.77

Weight (kDa)

10.48

Isoelectric Point (pI)

79.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 439
Acc36I ACCTGC 2 cut(s) 476, 516
AccI GTMKAC 1 cut(s) 460
AccII CGCG 1 cut(s) 391
AciI CCGC 1 cut(s) 405
AfiI CCNNNNNNNGG 1 cut(s) 411
AgsI TTSAA 1 cut(s) 32
AhlI ACTAGT 1 cut(s) 17
AjnI CCWGG 2 cut(s) 111, 422
AluBI AGCT 1 cut(s) 250
AluI AGCT 1 cut(s) 250
Alw26I GTCTC 2 cut(s) 250, 460
AlwNI CAGNNNCTG 1 cut(s) 508
AoxI GGCC 2 cut(s) 114, 420
ApeKI GCWGC 1 cut(s) 399
AspLEI GCGC 3 cut(s) 391, 399, 404
AspS9I GGNCC 2 cut(s) 42, 436
AsuHPI GGTGA 2 cut(s) 166, 209
AsuII TTCGAA 1 cut(s) 295
AvaII GGWCC 2 cut(s) 42, 436
BaeGI GKGCMC 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 292
BbvI GCAGC 1 cut(s) 386
BccI CCATC 2 cut(s) 128, 179
BceAI ACGGC 2 cut(s) 402, 410
BcgI CGANNNNNNTGC 2 cut(s) 381, 415
BciT130I CCWGG 2 cut(s) 113, 424
BclI TGATCA 1 cut(s) 139
BcoDI GTCTC 2 cut(s) 250, 460
BcuI ACTAGT 1 cut(s) 17
BfaI CTAG 3 cut(s) 18, 242, 335
BfoI RGCGCY 1 cut(s) 400
BfuAI ACCTGC 2 cut(s) 476, 516
BisI GCNGC 2 cut(s) 400, 405
BlsI GCNGC 2 cut(s) 401, 406
Bme1390I CCNGG 2 cut(s) 113, 424
Bme18I GGWCC 2 cut(s) 42, 436
BmgT120I GGNCC 2 cut(s) 42, 436
BmrFI CCNGG 2 cut(s) 113, 424
BpiI GAAGAC 1 cut(s) 292
BplI GAGNNNNNCTC 2 cut(s) 274, 306
BpmI CTGGAG 1 cut(s) 95
Bpu10I CCTNAGC 1 cut(s) 375
Bpu14I TTCGAA 1 cut(s) 295
BpuEI CTTGAG 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 423
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse1I ACTGG 1 cut(s) 439
Bse8I GATNNNNATC 1 cut(s) 304
BseBI CCWGG 2 cut(s) 113, 424
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 2 cut(s) 13, 120
BseJI GATNNNNATC 1 cut(s) 304
BseLI CCNNNNNNNGG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 439
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 1 cut(s) 386
Bsh1236I CGCG 1 cut(s) 391
BshFI GGCC 2 cut(s) 116, 422
BslI CCNNNNNNNGG 1 cut(s) 411
BsmAI GTCTC 2 cut(s) 250, 460
BsmBI CGTCTC 2 cut(s) 250, 460
BsnI GGCC 2 cut(s) 116, 422
Bsp119I TTCGAA 1 cut(s) 295
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 2 cut(s) 139, 518
BspACI CCGC 1 cut(s) 405
BspANI GGCC 2 cut(s) 116, 422
BspFNI CGCG 1 cut(s) 391
BspMI ACCTGC 2 cut(s) 476, 516
BspT104I TTCGAA 1 cut(s) 295
BsrI ACTGG 1 cut(s) 439
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 2 cut(s) 139, 518
Bst2UI CCWGG 2 cut(s) 113, 424
Bst4CI ACNGT 4 cut(s) 153, 346, 481, 535
Bst6I CTCTTC 1 cut(s) 297
BstBI TTCGAA 1 cut(s) 295
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 13, 120
BstFNI CGCG 1 cut(s) 391
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 3 cut(s) 391, 399, 404
BstKTI GATC 2 cut(s) 142, 521
BstMAI GTCTC 2 cut(s) 250, 460
BstMBI GATC 2 cut(s) 139, 518
BstMWI GCNNNNNNNGC 4 cut(s) 326, 380, 408, 560
BstNI CCWGG 2 cut(s) 113, 424
BstSCI CCNGG 2 cut(s) 111, 422
BstSLI GKGCMC 1 cut(s) 17
BstUI CGCG 1 cut(s) 391
BstV1I GCAGC 1 cut(s) 386
BstV2I GAAGAC 1 cut(s) 292
BsuRI GGCC 2 cut(s) 116, 422
BtsCI GGATG 2 cut(s) 13, 120
BtsI GCAGTG 1 cut(s) 521
BtsIMutI CAGTG 1 cut(s) 521
BveI ACCTGC 2 cut(s) 476, 516
Cac8I GCNNGC 1 cut(s) 409
CaiI CAGNNNCTG 1 cut(s) 508
CfoI GCGC 3 cut(s) 391, 399, 404
Cfr13I GGNCC 2 cut(s) 42, 436
CviAII CATG 1 cut(s) 229
CviJI RGCY 6 cut(s) 76, 116, 166, 250, 320, 422
CviKI_1 RGCY 6 cut(s) 76, 116, 166, 250, 320, 422
DdeI CTNAG 1 cut(s) 375
DpnI GATC 2 cut(s) 141, 520
DpnII GATC 2 cut(s) 139, 518
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco147I AGGCCT 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 42, 436
EcoRII CCWGG 2 cut(s) 111, 422
EcoT22I ATGCAT 1 cut(s) 100
Esp3I CGTCTC 2 cut(s) 250, 460
FaeI CATG 1 cut(s) 232
FaiI YATR 5 cut(s) 40, 96, 193, 195, 230
FatI CATG 1 cut(s) 228
FauNDI CATATG 1 cut(s) 193
FbaI TGATCA 1 cut(s) 139
FblI GTMKAC 1 cut(s) 460
Fnu4HI GCNGC 2 cut(s) 400, 405
FokI GGATG 2 cut(s) 20, 107
Fsp4HI GCNGC 2 cut(s) 400, 405
FspBI CTAG 3 cut(s) 18, 242, 335
GlaI GCGC 3 cut(s) 390, 398, 403
GluI GCNGC 2 cut(s) 400, 405
GsuI CTGGAG 1 cut(s) 95
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 2 cut(s) 116, 422
HhaI GCGC 3 cut(s) 391, 399, 404
Hin1II CATG 1 cut(s) 232
Hin6I GCGC 3 cut(s) 389, 397, 402
HinP1I GCGC 3 cut(s) 389, 397, 402
HincII GTYRAC 2 cut(s) 349, 461
HindII GTYRAC 2 cut(s) 349, 461
HinfI GANTC 2 cut(s) 84, 457
HphI GGTGA 2 cut(s) 166, 209
Hpy166II GTNNAC 3 cut(s) 200, 349, 461
Hpy188I TCNGA 3 cut(s) 163, 316, 432
Hpy8I GTNNAC 3 cut(s) 200, 349, 461
Hpy99I CGWCG 2 cut(s) 396, 465
HpyCH4III ACNGT 4 cut(s) 153, 346, 481, 535
HpyCH4V TGCA 6 cut(s) 4, 98, 446, 477, 485, 511
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 380, 408, 560
HpyF3I CTNAG 1 cut(s) 375
Hsp92II CATG 1 cut(s) 232
HspAI GCGC 3 cut(s) 389, 397, 402
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 2 cut(s) 139, 518
LmnI GCTCC 2 cut(s) 237, 334
Lsp1109I GCAGC 1 cut(s) 386
MaeI CTAG 3 cut(s) 18, 242, 335
MalI GATC 2 cut(s) 141, 520
MboI GATC 2 cut(s) 139, 518
MboII GAAGA 4 cut(s) 276, 284, 297, 309
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 3 cut(s) 26, 55, 156
MlyI GAGTC 2 cut(s) 93, 466
MmeI TCCRAC 1 cut(s) 524
MnlI CCTC 8 cut(s) 4, 87, 127, 284, 300, 412, 464, 525
Mph1103I ATGCAT 1 cut(s) 100
MseI TTAA 2 cut(s) 54, 62
MspR9I CCNGG 2 cut(s) 113, 424
MteI GCGCNGCGC 1 cut(s) 400
MvaI CCWGG 2 cut(s) 113, 424
MvnI CGCG 1 cut(s) 391
MwoI GCNNNNNNNGC 4 cut(s) 326, 380, 408, 560
NdeI CATATG 1 cut(s) 193
NdeII GATC 2 cut(s) 139, 518
NlaIII CATG 1 cut(s) 232
NsiI ATGCAT 1 cut(s) 100
NspV TTCGAA 1 cut(s) 295
PceI AGGCCT 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 466
PflFI GACNNNGTC 1 cut(s) 69
PkrI GCNGC 2 cut(s) 401, 406
PleI GAGTC 2 cut(s) 92, 465
PpsI GAGTC 2 cut(s) 92, 465
Psp6I CCWGG 2 cut(s) 111, 422
PspGI CCWGG 2 cut(s) 111, 422
PspPI GGNCC 2 cut(s) 42, 436
PstNI CAGNNNCTG 1 cut(s) 508
PsyI GACNNNGTC 1 cut(s) 69
SalI GTCGAC 1 cut(s) 459
SaqAI TTAA 2 cut(s) 54, 62
SatI GCNGC 2 cut(s) 400, 405
Sau3AI GATC 2 cut(s) 139, 518
Sau96I GGNCC 2 cut(s) 42, 436
SchI GAGTC 2 cut(s) 93, 466
ScrFI CCNGG 2 cut(s) 113, 424
SduI GDGCHC 1 cut(s) 17
SfuI TTCGAA 1 cut(s) 295
SinI GGWCC 2 cut(s) 42, 436
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 17
Sse9I AATT 3 cut(s) 26, 55, 156
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 1 cut(s) 405
SspMI CTAG 3 cut(s) 18, 242, 335
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 2 cut(s) 111, 422
TaaI ACNGT 4 cut(s) 153, 346, 481, 535
TaqI TCGA 3 cut(s) 295, 460, 517
TaqII GACCGA 1 cut(s) 59
TasI AATT 3 cut(s) 26, 55, 156
TauI GCSGC 1 cut(s) 407
Tru1I TTAA 2 cut(s) 54, 62
Tru9I TTAA 2 cut(s) 54, 62
TscAI CASTG 1 cut(s) 528
TseI GCWGC 1 cut(s) 399
TspGWI ACGGA 4 cut(s) 224, 431, 449, 478
TspRI CASTG 1 cut(s) 528
Tth111I GACNNNGTC 1 cut(s) 69
VpaK11BI GGWCC 2 cut(s) 42, 436
XmiI GTMKAC 1 cut(s) 460
XspI CTAG 3 cut(s) 18, 242, 335
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.