Rmu_sc0013338.1_g000001

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013338.1
OG0019089
Physical Location & Seq
Reverse (-)
4664 .. 7032
2369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013338.1_g000001.1.cds

Sequence Viewer

Length: 612 bp
atggacgagagcttaatcgcagtccccttgcctcgtcacgtttacgaccctctcgtactctaccgcaccccgatacaattcctgctttgtaggcttagcctctacaaagaaccgaagaatgtggctcctgtgatggaatggcctgatgttcattacaggactttggcccgcctcatatccaagcatgcctggctctactctgagatgcttgctgctcttggttcagtttgcagtgtggctcctatgatggaatggcctgatgttcattacaggactttggcccacctcatatccaagcatgcctggctctactctgagatgcttgctgccgaaaccattgtttatcagaaggacaatctggtaagaccctacaacaatgctctttccacatctttcaatctccccatgcgcacagcccacctcaccctcggccctcccttctgtacctgcgccccgcgcaccctccctcgcagcacagccccgactccagcctcccagcctcgacccagactacctgcagccatgacacgcctgcagcatcagacccgatcaggaccagatcaccaacacatgcgccccagctcactgtccctttcctactcgccggcgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

203

Amino Acids

23.07

Weight (kDa)

9.41

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019089)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000493.1_g000061 Rmu_sc0013338.1_g000001
rosa_roxburghii Rroxscaffold_3G00221180
rosa_rugosa Rorug06G0103000
rosa_samantha Rh2AG116600 Rh6AG143100 Rh6BG143400 Rh6CG140000 Rh6DG127700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 410
Acc36I ACCTGC 2 cut(s) 455, 523
AccII CGCG 1 cut(s) 457
AciI CCGC 3 cut(s) 64, 169, 455
AfaI GTAC 2 cut(s) 57, 445
AgsI TTSAA 1 cut(s) 397
AjnI CCWGG 2 cut(s) 188, 302
AluBI AGCT 2 cut(s) 12, 582
AluI AGCT 2 cut(s) 12, 582
AoxI GGCC 5 cut(s) 140, 165, 254, 279, 430
ApeKI GCWGC 5 cut(s) 212, 326, 471, 518, 535
AspLEI GCGC 4 cut(s) 411, 452, 459, 576
AspS9I GGNCC 4 cut(s) 166, 280, 431, 554
AsuHPI GGTGA 2 cut(s) 415, 554
AvaII GGWCC 1 cut(s) 554
BbvI GCAGC 5 cut(s) 199, 313, 483, 530, 547
BccI CCATC 2 cut(s) 127, 241
BciT130I CCWGG 2 cut(s) 190, 304
BfmI CTRYAG 2 cut(s) 516, 533
BfuAI ACCTGC 2 cut(s) 455, 523
BisI GCNGC 5 cut(s) 213, 327, 472, 519, 536
BlpI GCTNAGC 1 cut(s) 95
BlsI GCNGC 5 cut(s) 214, 328, 473, 520, 537
Bme1390I CCNGG 2 cut(s) 190, 304
Bme18I GGWCC 1 cut(s) 554
BmgT120I GGNCC 4 cut(s) 166, 280, 431, 554
BmiI GGNNCC 2 cut(s) 126, 240
BmrFI CCNGG 2 cut(s) 190, 304
BmsI GCATC 3 cut(s) 195, 309, 547
BpmI CTGGAG 1 cut(s) 471
Bpu1102I GCTNAGC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 427
Bse118I RCCGGY 1 cut(s) 604
BseBI CCWGG 2 cut(s) 190, 304
BseDI CCNNGG 1 cut(s) 427
BseMII CTCAG 2 cut(s) 192, 306
BseXI GCAGC 5 cut(s) 199, 313, 483, 530, 547
BseYI CCCAGC 2 cut(s) 495, 578
Bsh1236I CGCG 1 cut(s) 457
BshFI GGCC 5 cut(s) 142, 167, 256, 281, 432
BsiSI CCGG 1 cut(s) 605
BslFI GGGAC 2 cut(s) 8, 574
BsmFI GGGAC 2 cut(s) 8, 574
BsnI GGCC 5 cut(s) 142, 167, 256, 281, 432
Bsp143I GATC 2 cut(s) 548, 559
Bsp1720I GCTNAGC 1 cut(s) 95
BspACI CCGC 3 cut(s) 64, 169, 455
BspANI GGCC 5 cut(s) 142, 167, 256, 281, 432
BspCNI CTCAG 2 cut(s) 193, 307
BspFNI CGCG 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 126, 240
BspMAI CTGCAG 2 cut(s) 520, 537
BspMI ACCTGC 2 cut(s) 455, 523
BsrFI RCCGGY 1 cut(s) 604
BssAI RCCGGY 1 cut(s) 604
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 2 cut(s) 548, 559
Bst2UI CCWGG 2 cut(s) 190, 304
Bst4CI ACNGT 1 cut(s) 588
BstC8I GCNNGC 7 cut(s) 169, 186, 210, 300, 324, 533, 606
BstDEI CTNAG 3 cut(s) 95, 201, 315
BstFNI CGCG 1 cut(s) 457
BstHHI GCGC 4 cut(s) 411, 452, 459, 576
BstKTI GATC 2 cut(s) 551, 562
BstMBI GATC 2 cut(s) 548, 559
BstMWI GCNNNNNNNGC 4 cut(s) 91, 190, 304, 456
BstNI CCWGG 2 cut(s) 190, 304
BstNSI RCATGY 3 cut(s) 188, 302, 574
BstSCI CCNGG 2 cut(s) 188, 302
BstSFI CTRYAG 2 cut(s) 516, 533
BstUI CGCG 1 cut(s) 457
BstV1I GCAGC 5 cut(s) 199, 313, 483, 530, 547
BsuRI GGCC 5 cut(s) 142, 167, 256, 281, 432
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 2 cut(s) 238, 584
BveI ACCTGC 2 cut(s) 455, 523
Cac8I GCNNGC 7 cut(s) 169, 186, 210, 300, 324, 533, 606
CfoI GCGC 4 cut(s) 411, 452, 459, 576
Cfr10I RCCGGY 1 cut(s) 604
Cfr13I GGNCC 4 cut(s) 166, 280, 431, 554
Csp6I GTAC 2 cut(s) 56, 444
CviAII CATG 5 cut(s) 185, 299, 406, 523, 571
CviQI GTAC 2 cut(s) 56, 444
DdeI CTNAG 3 cut(s) 95, 201, 315
DpnI GATC 2 cut(s) 550, 561
DpnII GATC 2 cut(s) 548, 559
Eco47I GGWCC 1 cut(s) 554
EcoRII CCWGG 2 cut(s) 188, 302
FaeI CATG 5 cut(s) 188, 302, 409, 526, 574
FaiI YATR 8 cut(s) 176, 186, 245, 290, 300, 407, 524, 572
FaqI GGGAC 2 cut(s) 8, 574
FatI CATG 5 cut(s) 184, 298, 405, 522, 570
FauI CCCGC 2 cut(s) 176, 462
Fnu4HI GCNGC 5 cut(s) 213, 327, 472, 519, 536
Fsp4HI GCNGC 5 cut(s) 213, 327, 472, 519, 536
FspAI RTGCGCAY 1 cut(s) 410
FspI TGCGCA 1 cut(s) 410
GlaI GCGC 4 cut(s) 410, 451, 458, 575
GluI GCNGC 5 cut(s) 213, 327, 472, 519, 536
GsaI CCCAGC 2 cut(s) 499, 582
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 5 cut(s) 142, 167, 256, 281, 432
HapII CCGG 1 cut(s) 605
HhaI GCGC 4 cut(s) 411, 452, 459, 576
Hin1II CATG 5 cut(s) 188, 302, 409, 526, 574
Hin6I GCGC 4 cut(s) 409, 450, 457, 574
HinP1I GCGC 4 cut(s) 409, 450, 457, 574
HinfI GANTC 1 cut(s) 484
HpaII CCGG 1 cut(s) 605
HphI GGTGA 2 cut(s) 415, 554
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 4 cut(s) 202, 316, 348, 543
Hpy188III TCNNGA 1 cut(s) 552
Hpy8I GTNNAC 1 cut(s) 43
HpyAV CCTTC 2 cut(s) 343, 448
HpyCH4III ACNGT 1 cut(s) 588
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 3 cut(s) 231, 518, 535
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 190, 304, 456
HpyF3I CTNAG 3 cut(s) 95, 201, 315
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 5 cut(s) 188, 302, 409, 526, 574
HspAI GCGC 4 cut(s) 409, 450, 457, 574
KroI GCCGGC 1 cut(s) 604
KroNI GCCGGC 1 cut(s) 606
Kzo9I GATC 2 cut(s) 548, 559
LmnI GCTCC 2 cut(s) 130, 244
Lsp1109I GCAGC 5 cut(s) 199, 313, 483, 530, 547
LweI GCATC 3 cut(s) 195, 309, 547
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 35
MalI GATC 2 cut(s) 550, 561
MboI GATC 2 cut(s) 548, 559
MboII GAAGA 1 cut(s) 127
MluCI AATT 1 cut(s) 77
MlyI GAGTC 1 cut(s) 478
MreI CGCCGGCG 1 cut(s) 604
MroNI GCCGGC 1 cut(s) 604
MseI TTAA 1 cut(s) 14
MspI CCGG 1 cut(s) 605
MspR9I CCNGG 2 cut(s) 190, 304
MvaI CCWGG 2 cut(s) 190, 304
MvnI CGCG 1 cut(s) 457
MwoI GCNNNNNNNGC 4 cut(s) 91, 190, 304, 456
NaeI GCCGGC 1 cut(s) 606
NdeII GATC 2 cut(s) 548, 559
NgoMIV GCCGGC 1 cut(s) 604
NlaIII CATG 5 cut(s) 188, 302, 409, 526, 574
NlaIV GGNNCC 2 cut(s) 126, 240
NmeAIII GCCGAG 1 cut(s) 408
NmuCI GTSAC 1 cut(s) 35
NsbI TGCGCA 1 cut(s) 410
NspI RCATGY 3 cut(s) 188, 302, 574
PaeI GCATGC 2 cut(s) 188, 302
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PdiI GCCGGC 1 cut(s) 606
PkrI GCNGC 5 cut(s) 214, 328, 473, 520, 537
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
Psp6I CCWGG 2 cut(s) 188, 302
PspFI CCCAGC 2 cut(s) 495, 578
PspGI CCWGG 2 cut(s) 188, 302
PspN4I GGNNCC 2 cut(s) 126, 240
PspPI GGNCC 4 cut(s) 166, 280, 431, 554
PstI CTGCAG 2 cut(s) 520, 537
RsaI GTAC 2 cut(s) 57, 445
RsaNI GTAC 2 cut(s) 56, 444
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 5 cut(s) 213, 327, 472, 519, 536
Sau3AI GATC 2 cut(s) 548, 559
Sau96I GGNCC 4 cut(s) 166, 280, 431, 554
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 2 cut(s) 190, 304
SetI ASST 7 cut(s) 14, 42, 288, 423, 449, 517, 584
SfaNI GCATC 3 cut(s) 195, 309, 547
SfcI CTRYAG 2 cut(s) 516, 533
SgrAI CRCCGGYG 1 cut(s) 604
SinI GGWCC 1 cut(s) 554
SphI GCATGC 2 cut(s) 188, 302
Sse9I AATT 1 cut(s) 77
SsiI CCGC 3 cut(s) 64, 169, 455
StyD4I CCNGG 2 cut(s) 188, 302
TaaI ACNGT 1 cut(s) 588
TaiI ACGT 1 cut(s) 42
TaqI TCGA 1 cut(s) 502
TasI AATT 1 cut(s) 77
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 2 cut(s) 238, 591
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 5 cut(s) 212, 326, 471, 518, 535
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 2 cut(s) 140, 254
TspRI CASTG 2 cut(s) 238, 591
VpaK11BI GGWCC 1 cut(s) 554
XceI RCATGY 3 cut(s) 188, 302, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.