Rh6CG140000

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0019089
Physical Location & Seq
Reverse (-)
18103812 .. 18104579
768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG140000.1

Sequence Viewer

Length: 315 bp
ATGGACGAGAGCTTAATCGCAGTCCCCTTGCCTCGTCACGTTTCGGAGATAGGTGCCAAAAATTGGGAAGTTGTCACCGGTAAACATCGAATATCAGTGATTTGGGTATGGTCAATACTTCATAACTCTCTTCCTCCTTGGTTCAGTGTGGCTCCTATGATGGAATGGCCTGATGTTCATTACAGGACTTTGGCCCGCCTCATATCCAAGCATGCCTGGCTCTACTCTGAGATGCTTGCTGCCGAAACCATTGTTTATCAGAAGGACAATCTGGTTTCTGTTTCAATCAGGACACTATTGGCAAGCCTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

104

Amino Acids

11.98

Weight (kDa)

8.13

Isoelectric Point (pI)

24.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 50 - 89 6.2e-06 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019089)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000493.1_g000061 Rmu_sc0013338.1_g000001
rosa_roxburghii Rroxscaffold_3G00221180
rosa_rugosa Rorug06G0103000
rosa_samantha Rh2AG116600 Rh6AG143100 Rh6BG143400 Rh6CG140000 Rh6DG127700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 53
AccB7I CCANNNNNTGG 1 cut(s) 63
AciI CCGC 1 cut(s) 196
AfiI CCNNNNNNNGG 1 cut(s) 63
AgeI ACCGGT 1 cut(s) 77
AgsI TTSAA 1 cut(s) 285
AjnI CCWGG 1 cut(s) 215
AluBI AGCT 1 cut(s) 12
AluI AGCT 1 cut(s) 12
AoxI GGCC 2 cut(s) 167, 192
ApeKI GCWGC 1 cut(s) 239
AsiGI ACCGGT 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 67
BanI GGYRCC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 226
BccI CCATC 1 cut(s) 154
BciT130I CCWGG 1 cut(s) 217
BisI GCNGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 217
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 2 cut(s) 55, 153
BmrFI CCNGG 1 cut(s) 217
BmsI GCATC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 137
BsaWI WCCGGW 1 cut(s) 77
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse118I RCCGGY 1 cut(s) 77
BseBI CCWGG 1 cut(s) 217
BseDI CCNNGG 1 cut(s) 137
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMII CTCAG 1 cut(s) 219
BseXI GCAGC 1 cut(s) 226
BshFI GGCC 2 cut(s) 169, 194
BshNI GGYRCC 1 cut(s) 53
BshTI ACCGGT 1 cut(s) 77
BsiSI CCGG 1 cut(s) 78
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 2 cut(s) 169, 194
BspACI CCGC 1 cut(s) 196
BspANI GGCC 2 cut(s) 169, 194
BspCNI CTCAG 1 cut(s) 220
BspLI GGNNCC 2 cut(s) 55, 153
BspT107I GGYRCC 1 cut(s) 53
BsrFI RCCGGY 1 cut(s) 77
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 137
BssT1I CCWWGG 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 135
BstC8I GCNNGC 4 cut(s) 196, 213, 237, 304
BstDEI CTNAG 1 cut(s) 228
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNI CCWGG 1 cut(s) 217
BstNSI RCATGY 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 226
BsuRI GGCC 2 cut(s) 169, 194
BtsIMutI CAGTG 2 cut(s) 102, 151
Cac8I GCNNGC 4 cut(s) 196, 213, 237, 304
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 193
CspAI ACCGGT 1 cut(s) 77
CviAII CATG 1 cut(s) 212
CviJI RGCY 6 cut(s) 12, 152, 169, 194, 220, 306
CviKI_1 RGCY 6 cut(s) 12, 152, 169, 194, 220, 306
DdeI CTNAG 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 215
EcoT14I CCWWGG 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 137
FaeI CATG 1 cut(s) 215
FaiI YATR 5 cut(s) 109, 123, 158, 203, 213
FaqI GGGAC 1 cut(s) 8
FatI CATG 1 cut(s) 211
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 240
HaeIII GGCC 2 cut(s) 169, 194
HapII CCGG 1 cut(s) 78
Hin1II CATG 1 cut(s) 215
HpaII CCGG 1 cut(s) 78
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 1 cut(s) 83
Hpy188I TCNGA 3 cut(s) 46, 229, 261
Hpy188III TCNNGA 1 cut(s) 289
Hpy8I GTNNAC 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 256
HpyCH4IV ACGT 1 cut(s) 39
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 1 cut(s) 228
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 1 cut(s) 215
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 7 cut(s) 91, 169, 183, 202, 229, 257, 274
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 1 cut(s) 222
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 2 cut(s) 35, 73
MboII GAAGA 1 cut(s) 122
MluCI AATT 1 cut(s) 61
MnlI CCTC 3 cut(s) 42, 144, 209
MseI TTAA 1 cut(s) 14
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 1 cut(s) 217
MvaI CCWGG 1 cut(s) 217
MwoI GCNNNNNNNGC 1 cut(s) 217
NlaIII CATG 1 cut(s) 215
NlaIV GGNNCC 2 cut(s) 55, 153
NmuCI GTSAC 2 cut(s) 35, 73
NspI RCATGY 1 cut(s) 215
PaeI GCATGC 1 cut(s) 215
PflMI CCANNNNNTGG 1 cut(s) 63
PinAI ACCGGT 1 cut(s) 77
PkrI GCNGC 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 215
PspGI CCWGG 1 cut(s) 215
PspN4I GGNNCC 2 cut(s) 55, 153
PspPI GGNCC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 1 cut(s) 240
Sau96I GGNCC 1 cut(s) 193
ScrFI CCNGG 1 cut(s) 217
SetI ASST 3 cut(s) 14, 42, 55
SfaNI GCATC 1 cut(s) 222
SphI GCATGC 1 cut(s) 215
Sse9I AATT 1 cut(s) 61
SsiI CCGC 1 cut(s) 196
StyD4I CCNGG 1 cut(s) 215
StyI CCWWGG 1 cut(s) 137
TaiI ACGT 1 cut(s) 42
TaqI TCGA 1 cut(s) 88
TasI AATT 1 cut(s) 61
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 2 cut(s) 102, 151
TseFI GTSAC 2 cut(s) 35, 73
TseI GCWGC 1 cut(s) 239
Tsp45I GTSAC 2 cut(s) 35, 73
TspDTI ATGAA 2 cut(s) 110, 167
TspRI CASTG 2 cut(s) 102, 151
Van91I CCANNNNNTGG 1 cut(s) 63
XceI RCATGY 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.