Rh6AG143100

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0019089
Physical Location & Seq
Reverse (-)
22231449 .. 22262215
30767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG143100.1

Sequence Viewer

Length: 552 bp
ATGGAGAGGCAGATGGTTGAAAGACTTAATTGGATGGCTGATCAATTAAAGAAAGGCCTTCATCAAAGTGGAGGTATCATGGTGAACTATGTTTACAGTGTGGCTCCTATGATGGAATGGCCTGATGTTCATTACAGGACTTTGGCCCGCCTCATAACCAAGCATGCCTGGCTCTACTCTGAGATGCTTGCTGCCGAAACCATTGTTTATCAGAAGGACAATCTGAACACCAATCAGAAACTTAAGAGCCAGAACAAATGCAGAATCAGCTCGAAGAATCAGAGATTCAGAATGCCTAGCAACAGCAAGCCAATTTCTAGGCAAAACTCTTCAATTCTTGCTCCGGTTGCAAAACCCTCTTCAAACGTTTTGGGCATTCTCAATTTCTCTCGAAACCATCTAACCATTCCAATTCCTACTCAAATTCGCAAGACGGCCTTTGCTGGAACTCGTCCTTTGCTGGAAATCTGGATCGCAAGACGGAAGTCAATTTCTAGTGTTTCAGCAACTCGTCCTTTGCTGGAAACCCACAAAGCATTGGTACCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

183

Amino Acids

20.94

Weight (kDa)

10.61

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCD PF05292 6 - 32 1.1e-06 Malonyl-CoA decarboxylase C-terminal domain
Dus PF01207 34 - 80 6e-06 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019089)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000493.1_g000061 Rmu_sc0013338.1_g000001
rosa_roxburghii Rroxscaffold_3G00221180
rosa_rugosa Rorug06G0103000
rosa_samantha Rh2AG116600 Rh6AG143100 Rh6BG143400 Rh6CG140000 Rh6DG127700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 541
AccB1I GGYRCC 1 cut(s) 541
AciI CCGC 1 cut(s) 148
AclI AACGTT 1 cut(s) 366
AclWI GGATC 1 cut(s) 479
AcsI RAATTY 1 cut(s) 423
AfaI GTAC 1 cut(s) 543
AflII CTTAAG 1 cut(s) 242
AgsI TTSAA 3 cut(s) 20, 333, 363
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 1 cut(s) 270
AluI AGCT 1 cut(s) 270
AlwI GGATC 1 cut(s) 479
AoxI GGCC 4 cut(s) 55, 119, 144, 435
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 1 cut(s) 423
Asp718I GGTACC 1 cut(s) 541
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 1 cut(s) 94
BanI GGYRCC 1 cut(s) 541
BbvI GCAGC 1 cut(s) 178
BccI CCATC 4 cut(s) 7, 28, 106, 405
BceAI ACGGC 1 cut(s) 450
BciT130I CCWGG 1 cut(s) 169
BclI TGATCA 1 cut(s) 40
BfaI CTAG 3 cut(s) 297, 318, 495
BfrI CTTAAG 1 cut(s) 242
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
Bme1390I CCNGG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 2 cut(s) 105, 543
BmrFI CCNGG 1 cut(s) 169
BmsI GCATC 1 cut(s) 174
BoxI GACNNNNGTC 1 cut(s) 484
BsaWI WCCGGW 1 cut(s) 343
BseBI CCWGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 39
BseMII CTCAG 1 cut(s) 171
BseXI GCAGC 1 cut(s) 178
BshFI GGCC 4 cut(s) 57, 121, 146, 437
BshNI GGYRCC 1 cut(s) 541
BsiSI CCGG 1 cut(s) 344
BsmI GAATGC 2 cut(s) 297, 375
BsnI GGCC 4 cut(s) 57, 121, 146, 437
Bsp143I GATC 2 cut(s) 40, 471
BspACI CCGC 1 cut(s) 148
BspANI GGCC 4 cut(s) 57, 121, 146, 437
BspCNI CTCAG 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 105, 543
BspPI GGATC 1 cut(s) 479
BspT107I GGYRCC 1 cut(s) 541
BspTI CTTAAG 1 cut(s) 242
BssMI GATC 2 cut(s) 40, 471
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 1 cut(s) 98
Bst6I CTCTTC 2 cut(s) 334, 364
BstAFI CTTAAG 1 cut(s) 242
BstC8I GCNNGC 4 cut(s) 148, 165, 189, 308
BstDEI CTNAG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 39
BstKTI GATC 2 cut(s) 43, 474
BstMBI GATC 2 cut(s) 40, 471
BstMWI GCNNNNNNNGC 3 cut(s) 169, 267, 347
BstNI CCWGG 1 cut(s) 169
BstNSI RCATGY 1 cut(s) 167
BstPAI GACNNNNGTC 1 cut(s) 484
BstSCI CCNGG 1 cut(s) 167
BstV1I GCAGC 1 cut(s) 178
BsuRI GGCC 4 cut(s) 57, 121, 146, 437
BtsCI GGATG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 103
Cac8I GCNNGC 4 cut(s) 148, 165, 189, 308
Cfr13I GGNCC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 542
CviAII CATG 3 cut(s) 79, 164, 549
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 1 cut(s) 180
DpnI GATC 2 cut(s) 42, 473
DpnII GATC 2 cut(s) 40, 471
Eam1104I CTCTTC 2 cut(s) 334, 364
EarI CTCTTC 2 cut(s) 334, 364
Eco147I AGGCCT 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 167
FaeI CATG 3 cut(s) 82, 167, 552
FaiI YATR 6 cut(s) 80, 90, 110, 155, 165, 550
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FatI CATG 3 cut(s) 78, 163, 548
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 1 cut(s) 46
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 3 cut(s) 297, 318, 495
GluI GCNGC 1 cut(s) 192
HaeIII GGCC 4 cut(s) 57, 121, 146, 437
HapII CCGG 1 cut(s) 344
Hin1II CATG 3 cut(s) 82, 167, 552
HinfI GANTC 3 cut(s) 264, 277, 285
HpaII CCGG 1 cut(s) 344
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 85, 94
Hpy188I TCNGA 6 cut(s) 181, 213, 225, 237, 282, 290
Hpy188III TCNNGA 2 cut(s) 390, 469
Hpy8I GTNNAC 2 cut(s) 85, 94
HpyAV CCTTC 2 cut(s) 68, 208
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 2 cut(s) 261, 350
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 267, 347
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 3 cut(s) 82, 167, 552
KpnI GGTACC 1 cut(s) 545
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 2 cut(s) 40, 471
LmnI GCTCC 2 cut(s) 109, 346
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 1 cut(s) 174
MaeI CTAG 3 cut(s) 297, 318, 495
MaeII ACGT 1 cut(s) 366
MalI GATC 2 cut(s) 42, 473
MboI GATC 2 cut(s) 40, 471
MboII GAAGA 3 cut(s) 286, 321, 351
MluCI AATT 8 cut(s) 28, 44, 312, 333, 382, 411, 423, 489
MnlI CCTC 3 cut(s) 65, 161, 367
MseI TTAA 3 cut(s) 27, 47, 243
MslI CAYNNNNRTG 1 cut(s) 66
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 169
Mva1269I GAATGC 2 cut(s) 297, 375
MvaI CCWGG 1 cut(s) 169
MwoI GCNNNNNNNGC 3 cut(s) 169, 267, 347
NdeII GATC 2 cut(s) 40, 471
NlaIII CATG 3 cut(s) 82, 167, 552
NlaIV GGNNCC 2 cut(s) 105, 543
NspI RCATGY 1 cut(s) 167
PaeI GCATGC 1 cut(s) 167
PceI AGGCCT 1 cut(s) 57
PctI GAATGC 2 cut(s) 297, 375
PfeI GAWTC 3 cut(s) 264, 277, 285
PkrI GCNGC 1 cut(s) 193
PshAI GACNNNNGTC 1 cut(s) 484
Psp1406I AACGTT 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 167
PspGI CCWGG 1 cut(s) 167
PspN4I GGNNCC 2 cut(s) 105, 543
PspPI GGNCC 1 cut(s) 145
PsrI GAACNNNNNNTAC 2 cut(s) 77, 109
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
RseI CAYNNNNRTG 1 cut(s) 66
SaqAI TTAA 3 cut(s) 27, 47, 243
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 2 cut(s) 40, 471
Sau96I GGNCC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 169
SetI ASST 4 cut(s) 76, 272, 369, 547
SfaNI GCATC 1 cut(s) 174
SmiMI CAYNNNNRTG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SphI GCATGC 1 cut(s) 167
Sse9I AATT 8 cut(s) 28, 44, 312, 333, 382, 411, 423, 489
SseBI AGGCCT 1 cut(s) 57
SsiI CCGC 1 cut(s) 148
SspMI CTAG 3 cut(s) 297, 318, 495
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 167
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 1 cut(s) 369
TaqI TCGA 2 cut(s) 272, 391
TasI AATT 8 cut(s) 28, 44, 312, 333, 382, 411, 423, 489
TfiI GAWTC 3 cut(s) 264, 277, 285
Tru1I TTAA 3 cut(s) 27, 47, 243
Tru9I TTAA 3 cut(s) 27, 47, 243
TscAI CASTG 1 cut(s) 103
TseI GCWGC 1 cut(s) 191
TspDTI ATGAA 2 cut(s) 50, 119
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 1 cut(s) 103
Vha464I CTTAAG 1 cut(s) 242
XapI RAATTY 1 cut(s) 423
XceI RCATGY 1 cut(s) 167
XspI CTAG 3 cut(s) 297, 318, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.