Rmu_sc0034428.1_g000002

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0034428.1
OG0017267
Physical Location & Seq
Reverse (-)
1819 .. 4077
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0034428.1_g000002.1.cds

Sequence Viewer

Length: 987 bp
atgcccaagcctgaatactttaatggccaaaatgcaattgttgcggtcaatgtgcacctaggatataaccaagaagattctattgtgatgaaccgcgcatctgtgcaacgtggtgcatatctgcagagtggtgatactattattgcaaggtgcagtgaatctggtactgatcatagcatcaaactgaggcatactgaaagaggaaaggttcagacggttcgttctccttgccttggagacaagttctcaagcatgcatgggcagaagggtgtcctaggctttttggagtctgaagagaacttccccttcacaatacaggggatagttccagatatagtaattaatccacatgcatttccttcacgacaagctcctggtcaactcctagaggctgctttagggaaggagattgcctgtggtggctcaatgagatatgcaactcctttctctactccctctgttgatgacatcacagatcagcttcatagagctggatttcaaagatggggaaatgagagagtatacagtggtcaaactggtaaaatggttcgccaactgatatttatgggtccaacattctatcaacgtctcattcacatgtctgaagacaaagtgaagttcaggaacactgggccagttcaccctctcacccggcagccagtggcagacagaaagagatttggtggggtaaagtttggtgagatggagcgcgactgccttatagcccatggagcatcagcaaacttacatgaacgccttttcactcttagtgactcctccgaaatgcatgcttgcctgaaatgcaaaaatgcggctaatgtgatagatggaacagttgatggcagtagaatcaggggtccatattgccgggcctgtaagtctgttgatgacattgtcaggttgaatgttccatatggggccaagttattgtgccaggaacttttcagcatgggcataagtctcaagtttgagaccaggctctgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

328

Amino Acids

36.38

Weight (kDa)

8.88

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 522
AccII CGCG 2 cut(s) 96, 711
AciI CCGC 3 cut(s) 44, 94, 812
AcoI YGGCCR 1 cut(s) 25
AcuI CTGAAG 2 cut(s) 312, 624
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 233
AflIII ACRYGT 1 cut(s) 597
AgsI TTSAA 2 cut(s) 500, 904
AjnI CCWGG 3 cut(s) 373, 933, 974
AluBI AGCT 3 cut(s) 371, 481, 491
AluI AGCT 3 cut(s) 371, 481, 491
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 4 cut(s) 231, 593, 965, 965
Alw44I GTGCAC 1 cut(s) 53
AlwNI CAGNNNCTG 1 cut(s) 981
AoxI GGCC 4 cut(s) 25, 632, 870, 918
ApaLI GTGCAC 1 cut(s) 53
ApeKI GCWGC 2 cut(s) 392, 655
AseI ATTAAT 1 cut(s) 342
AspA2I CCTAGG 2 cut(s) 58, 274
AspLEI GCGC 2 cut(s) 98, 711
AspS9I GGNCC 5 cut(s) 569, 632, 857, 870, 918
AsuC2I CCSGG 2 cut(s) 652, 869
AsuHPI GGTGA 4 cut(s) 143, 632, 640, 710
AvaII GGWCC 2 cut(s) 569, 857
AvrII CCTAGG 2 cut(s) 58, 274
BaeGI GKGCMC 1 cut(s) 57
BalI TGGCCA 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 2 cut(s) 379, 667
BccI CCATC 4 cut(s) 498, 697, 821, 833
BcgI CGANNNNNNTGC 2 cut(s) 770, 804
BciT130I CCWGG 3 cut(s) 375, 935, 976
BclI TGATCA 1 cut(s) 169
BcnI CCSGG 2 cut(s) 652, 869
BcoDI GTCTC 4 cut(s) 231, 593, 965, 965
BfaI CTAG 3 cut(s) 59, 275, 386
BfmI CTRYAG 1 cut(s) 122
BisI GCNGC 3 cut(s) 393, 656, 813
BlnI CCTAGG 2 cut(s) 58, 274
BlsI GCNGC 3 cut(s) 394, 657, 814
Bme1390I CCNGG 5 cut(s) 375, 652, 869, 935, 976
Bme18I GGWCC 2 cut(s) 569, 857
BmgT120I GGNCC 5 cut(s) 569, 632, 857, 870, 918
BmiI GGNNCC 3 cut(s) 570, 858, 919
BmrFI CCNGG 5 cut(s) 375, 652, 869, 935, 976
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 3 cut(s) 107, 186, 743
BmuI ACTGGG 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 612
BpuEI CTTGAG 2 cut(s) 232, 947
BpuMI CCSGG 2 cut(s) 652, 869
BsaI GGTCTC 1 cut(s) 965
BsaJI CCNNGG 4 cut(s) 58, 232, 274, 727
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 4 cut(s) 541, 634, 635, 659
BseBI CCWGG 3 cut(s) 375, 935, 976
BseDI CCNNGG 4 cut(s) 58, 232, 274, 727
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 4 cut(s) 541, 634, 635, 659
BseRI GAGGAG 1 cut(s) 766
BseSI GKGCMC 1 cut(s) 57
BseXI GCAGC 2 cut(s) 379, 667
BsgI GTGCAG 1 cut(s) 172
Bsh1236I CGCG 2 cut(s) 96, 711
BshFI GGCC 4 cut(s) 27, 634, 872, 920
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 2 cut(s) 652, 868
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 4 cut(s) 231, 593, 965, 965
BsmBI CGTCTC 1 cut(s) 593
BsnI GGCC 4 cut(s) 27, 634, 872, 920
Bso31I GGTCTC 1 cut(s) 965
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 169, 475
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 3 cut(s) 44, 94, 812
BspANI GGCC 4 cut(s) 27, 634, 872, 920
BspCNI CTCAG 1 cut(s) 177
BspFNI CGCG 2 cut(s) 96, 711
BspLI GGNNCC 3 cut(s) 570, 858, 919
BspMAI CTGCAG 1 cut(s) 126
BspTNI GGTCTC 1 cut(s) 965
BsrI ACTGG 4 cut(s) 541, 634, 635, 659
BssECI CCNNGG 4 cut(s) 58, 232, 274, 727
BssMI GATC 2 cut(s) 169, 475
BssNAI GTATAC 1 cut(s) 523
BssT1I CCWWGG 4 cut(s) 58, 232, 274, 727
Bst1107I GTATAC 1 cut(s) 523
Bst2UI CCWGG 3 cut(s) 375, 935, 976
Bst4CI ACNGT 3 cut(s) 217, 527, 835
Bst6I CTCTTC 1 cut(s) 288
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 3 cut(s) 254, 789, 793
BstDEI CTNAG 2 cut(s) 185, 767
BstDSI CCRYGG 1 cut(s) 727
BstFNI CGCG 2 cut(s) 96, 711
BstHHI GCGC 2 cut(s) 98, 711
BstKTI GATC 2 cut(s) 172, 478
BstMAI GTCTC 4 cut(s) 231, 593, 965, 965
BstMBI GATC 2 cut(s) 169, 475
BstMWI GCNNNNNNNGC 3 cut(s) 41, 731, 801
BstNI CCWGG 3 cut(s) 375, 935, 976
BstNSI RCATGY 4 cut(s) 256, 353, 601, 791
BstSCI CCNGG 5 cut(s) 373, 650, 867, 933, 974
BstSFI CTRYAG 1 cut(s) 122
BstSLI GKGCMC 1 cut(s) 57
BstUI CGCG 2 cut(s) 96, 711
BstV1I GCAGC 2 cut(s) 379, 667
BstV2I GAAGAC 1 cut(s) 612
BstZ17I GTATAC 1 cut(s) 523
BsuRI GGCC 4 cut(s) 27, 634, 872, 920
BtgI CCRYGG 1 cut(s) 727
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 4 cut(s) 160, 532, 627, 666
Cac8I GCNNGC 3 cut(s) 254, 789, 793
CaiI CAGNNNCTG 1 cut(s) 981
CfoI GCGC 2 cut(s) 98, 711
Cfr13I GGNCC 5 cut(s) 569, 632, 857, 870, 918
Csp6I GTAC 1 cut(s) 165
CviAII CATG 8 cut(s) 253, 257, 350, 598, 728, 749, 788, 949
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 2 cut(s) 185, 767
DpnI GATC 2 cut(s) 171, 477
DpnII GATC 2 cut(s) 169, 475
EaeI YGGCCR 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco130I CCWWGG 4 cut(s) 58, 232, 274, 727
Eco31I GGTCTC 1 cut(s) 965
Eco47I GGWCC 2 cut(s) 569, 857
Eco57I CTGAAG 2 cut(s) 312, 624
EcoRII CCWGG 3 cut(s) 373, 933, 974
EcoT14I CCWWGG 4 cut(s) 58, 232, 274, 727
EcoT22I ATGCAT 3 cut(s) 258, 355, 789
ErhI CCWWGG 4 cut(s) 58, 232, 274, 727
Esp3I CGTCTC 1 cut(s) 593
FaeI CATG 8 cut(s) 256, 260, 353, 601, 731, 752, 791, 952
FatI CATG 8 cut(s) 252, 256, 349, 597, 727, 748, 787, 948
FauNDI CATATG 1 cut(s) 913
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 3 cut(s) 393, 656, 813
Fsp4HI GCNGC 3 cut(s) 393, 656, 813
FspBI CTAG 3 cut(s) 59, 275, 386
GlaI GCGC 2 cut(s) 97, 710
GluI GCNGC 3 cut(s) 393, 656, 813
HaeIII GGCC 4 cut(s) 27, 634, 872, 920
HapII CCGG 2 cut(s) 652, 868
HhaI GCGC 2 cut(s) 98, 711
Hin1II CATG 8 cut(s) 256, 260, 353, 601, 731, 752, 791, 952
Hin6I GCGC 2 cut(s) 96, 709
HinP1I GCGC 2 cut(s) 96, 709
HincII GTYRAC 1 cut(s) 380
HindII GTYRAC 1 cut(s) 380
HinfI GANTC 5 cut(s) 77, 158, 287, 773, 849
HpaII CCGG 2 cut(s) 652, 868
HphI GGTGA 4 cut(s) 143, 632, 640, 710
Hpy166II GTNNAC 4 cut(s) 55, 380, 523, 640
Hpy188I TCNGA 4 cut(s) 213, 292, 604, 781
Hpy188III TCNNGA 3 cut(s) 329, 363, 622
Hpy8I GTNNAC 4 cut(s) 55, 380, 523, 640
HpyAV CCTTC 4 cut(s) 259, 316, 369, 397
HpyCH4III ACNGT 3 cut(s) 217, 527, 835
HpyCH4IV ACGT 2 cut(s) 109, 586
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 731, 801
HpyF3I CTNAG 2 cut(s) 185, 767
HpySE526I ACGT 2 cut(s) 109, 586
Hsp92II CATG 8 cut(s) 256, 260, 353, 601, 731, 752, 791, 952
HspAI GCGC 2 cut(s) 96, 709
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 2 cut(s) 169, 475
LmnI GCTCC 3 cut(s) 376, 706, 731
Lsp1109I GCAGC 2 cut(s) 379, 667
LweI GCATC 3 cut(s) 107, 186, 743
MaeI CTAG 3 cut(s) 59, 275, 386
MaeII ACGT 2 cut(s) 109, 586
MaeIII GTNAC 1 cut(s) 770
MalI GATC 2 cut(s) 171, 477
MboI GATC 2 cut(s) 169, 475
MboII GAAGA 3 cut(s) 86, 305, 617
MfeI CAATTG 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 57
MlsI TGGCCA 1 cut(s) 27
MluCI AATT 2 cut(s) 36, 339
MluNI TGGCCA 1 cut(s) 27
MlyI GAGTC 2 cut(s) 296, 767
MmeI TCCRAC 1 cut(s) 596
MnlI CCTC 6 cut(s) 180, 194, 382, 466, 654, 787
Mox20I TGGCCA 1 cut(s) 27
Mph1103I ATGCAT 3 cut(s) 258, 355, 789
MscI TGGCCA 1 cut(s) 27
MseI TTAA 2 cut(s) 21, 342
MslI CAYNNNNRTG 1 cut(s) 596
Msp20I TGGCCA 1 cut(s) 27
MspI CCGG 2 cut(s) 652, 868
MspR9I CCNGG 5 cut(s) 375, 652, 869, 935, 976
MunI CAATTG 1 cut(s) 36
MvaI CCWGG 3 cut(s) 375, 935, 976
MvnI CGCG 2 cut(s) 96, 711
MwoI GCNNNNNNNGC 3 cut(s) 41, 731, 801
NciI CCSGG 2 cut(s) 652, 869
NcoI CCATGG 1 cut(s) 727
NdeI CATATG 1 cut(s) 913
NdeII GATC 2 cut(s) 169, 475
NlaIII CATG 8 cut(s) 256, 260, 353, 601, 731, 752, 791, 952
NlaIV GGNNCC 3 cut(s) 570, 858, 919
NmuCI GTSAC 1 cut(s) 770
NsiI ATGCAT 3 cut(s) 258, 355, 789
NspI RCATGY 4 cut(s) 256, 353, 601, 791
PaeI GCATGC 2 cut(s) 256, 791
PciI ACATGT 1 cut(s) 597
PfeI GAWTC 3 cut(s) 77, 158, 849
PflFI GACNNNGTC 1 cut(s) 893
PkrI GCNGC 3 cut(s) 394, 657, 814
PleI GAGTC 2 cut(s) 295, 767
PpsI GAGTC 2 cut(s) 295, 767
PscI ACATGT 1 cut(s) 597
PshBI ATTAAT 1 cut(s) 342
Psp6I CCWGG 3 cut(s) 373, 933, 974
PspGI CCWGG 3 cut(s) 373, 933, 974
PspN4I GGNNCC 3 cut(s) 570, 858, 919
PspPI GGNCC 5 cut(s) 569, 632, 857, 870, 918
PstI CTGCAG 1 cut(s) 126
PstNI CAGNNNCTG 1 cut(s) 981
PsyI GACNNNGTC 1 cut(s) 893
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
RseI CAYNNNNRTG 1 cut(s) 596
SaqAI TTAA 2 cut(s) 21, 342
SatI GCNGC 3 cut(s) 393, 656, 813
Sau3AI GATC 2 cut(s) 169, 475
Sau96I GGNCC 5 cut(s) 569, 632, 857, 870, 918
SchI GAGTC 2 cut(s) 296, 767
ScrFI CCNGG 5 cut(s) 375, 652, 869, 935, 976
SduI GDGCHC 1 cut(s) 57
SetI ASST 9 cut(s) 60, 112, 152, 210, 373, 483, 493, 589, 902
SfaNI GCATC 3 cut(s) 107, 186, 743
SfcI CTRYAG 1 cut(s) 122
SinI GGWCC 2 cut(s) 569, 857
SmiMI CAYNNNNRTG 1 cut(s) 596
SmlI CTYRAG 2 cut(s) 247, 962
SmoI CTYRAG 2 cut(s) 247, 962
SphI GCATGC 2 cut(s) 256, 791
Sse9I AATT 2 cut(s) 36, 339
SsiI CCGC 3 cut(s) 44, 94, 812
SspMI CTAG 3 cut(s) 59, 275, 386
StyD4I CCNGG 5 cut(s) 373, 650, 867, 933, 974
StyI CCWWGG 4 cut(s) 58, 232, 274, 727
TaaI ACNGT 3 cut(s) 217, 527, 835
TaiI ACGT 2 cut(s) 112, 589
TasI AATT 2 cut(s) 36, 339
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 3 cut(s) 77, 158, 849
Tru1I TTAA 2 cut(s) 21, 342
Tru9I TTAA 2 cut(s) 21, 342
TscAI CASTG 4 cut(s) 160, 532, 634, 666
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 2 cut(s) 392, 655
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 3 cut(s) 104, 473, 765
TspRI CASTG 4 cut(s) 160, 532, 634, 666
Tth111I GACNNNGTC 1 cut(s) 893
VneI GTGCAC 1 cut(s) 53
VpaK11BI GGWCC 2 cut(s) 569, 857
VspI ATTAAT 1 cut(s) 342
XceI RCATGY 4 cut(s) 256, 353, 601, 791
XmaJI CCTAGG 2 cut(s) 58, 274
XmiI GTMKAC 1 cut(s) 522
XspI CTAG 3 cut(s) 59, 275, 386
Zsp2I ATGCAT 3 cut(s) 258, 355, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.