Rmu_ssc0000136.1_g000016

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000136.1
OG0018966
Physical Location & Seq
Forward (+)
62210 .. 66455
4246 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
atgaggaaaatccagtttgctgcaactatgacattcagtgtccgaatcatgattgctatacctttccagaaagggaacgacgtcaggaacaaaacttgtcgctttgccaccacgtgtgtgctttttcagacttcggggtcatttagggcctcaaaactgcaatttactatttttcagaattttcggctttctagccctgcccttgttgttgatgttcccagggatagcaacataactgctgaagtgatggagtccttaaatattcccaagagtactcgccgagtacttttcaggacattaaatactgataggggtcttatgcataaaaaggaatttgactcaagctttgtgggattcacggaggatggagcgaaatgggtggttaagaacacggacatcaaacttgttggaactgattacttttccattgctacctatgatgaagctattccggcacatattgcttttctaaagggaagggaaatcatacctgtggaaggcctaaagcttgatggcataaaaccaggaaattattctgtgcattgcttacctctaaggttgcttggtgctgaaggagcaccaacaagatgcattctcatcaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

201

Amino Acids

22.53

Weight (kDa)

9.69

Isoelectric Point (pI)

33.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 63 - 143 5.4e-06 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 136
AatII GACGTC 1 cut(s) 84
AcsI RAATTY 2 cut(s) 178, 332
AcuI CTGAAG 2 cut(s) 261, 591
AcvI CACGTG 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 81
AdeI CACNNNGTG 1 cut(s) 114
AfaI GTAC 2 cut(s) 274, 285
AfiI CCNNNNNNNGG 1 cut(s) 497
AflIII ACRYGT 1 cut(s) 113
AjnI CCWGG 2 cut(s) 218, 523
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 3 cut(s) 345, 446, 508
AluI AGCT 3 cut(s) 345, 446, 508
Alw21I GWGCWC 1 cut(s) 580
AoxI GGCC 2 cut(s) 147, 499
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 2 cut(s) 178, 332
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
Asp700I GAANNNNTTC 2 cut(s) 447, 532
AspS9I GGNCC 1 cut(s) 147
BbrPI CACGTG 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 1 cut(s) 7
BccI CCATC 3 cut(s) 241, 359, 506
BciT130I CCWGG 2 cut(s) 220, 525
BfaI CTAG 1 cut(s) 192
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
BmcAI AGTACT 2 cut(s) 274, 285
Bme1390I CCNGG 2 cut(s) 220, 525
BmgT120I GGNCC 1 cut(s) 147
BmrFI CCNGG 2 cut(s) 220, 525
BmsI GCATC 1 cut(s) 578
BpuEI CTTGAG 1 cut(s) 325
BsaAI YACGTR 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 218, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse1I ACTGG 1 cut(s) 13
Bse3DI GCAATG 2 cut(s) 426, 541
BseBI CCWGG 2 cut(s) 220, 525
BseDI CCNNGG 2 cut(s) 218, 219
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMI GCAATG 2 cut(s) 426, 541
BseNI ACTGG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 7
BshFI GGCC 2 cut(s) 149, 501
BsiHKAI GWGCWC 1 cut(s) 580
BsiSI CCGG 1 cut(s) 452
BslI CCNNNNNNNGG 1 cut(s) 497
BsmI GAATGC 1 cut(s) 591
BsnI GGCC 2 cut(s) 149, 501
Bsp1286I GDGCHC 1 cut(s) 580
BspANI GGCC 2 cut(s) 149, 501
BspHI TCATGA 1 cut(s) 48
BsrDI GCAATG 2 cut(s) 426, 541
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 218, 219
BssNI GRCGYC 1 cut(s) 81
Bst2UI CCWGG 2 cut(s) 220, 525
BstACI GRCGYC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 461
BstBAI YACGTR 1 cut(s) 114
BstDEI CTNAG 1 cut(s) 554
BstENI CCTNNNNNAGG 1 cut(s) 495
BstF5I GGATG 1 cut(s) 370
BstMWI GCNNNNNNNGC 3 cut(s) 452, 461, 575
BstNI CCWGG 2 cut(s) 220, 525
BstSCI CCNGG 2 cut(s) 218, 523
BstV1I GCAGC 1 cut(s) 7
BsuRI GGCC 2 cut(s) 149, 501
BtsCI GGATG 1 cut(s) 370
BtsIMutI CAGTG 1 cut(s) 43
CciI TCATGA 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 147
Csp6I GTAC 2 cut(s) 273, 284
CspCI CAANNNNNGTGG 2 cut(s) 330, 365
CviAII CATG 1 cut(s) 49
CviJI RGCY 7 cut(s) 149, 187, 195, 345, 446, 501, 508
CviKI_1 RGCY 7 cut(s) 149, 187, 195, 345, 446, 501, 508
CviQI GTAC 2 cut(s) 273, 284
DdeI CTNAG 1 cut(s) 554
DraIII CACNNNGTG 1 cut(s) 114
DrdI GACNNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 501
Eco57I CTGAAG 2 cut(s) 261, 591
Eco72I CACGTG 1 cut(s) 114
EcoNI CCTNNNNNAGG 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 218, 523
EcoT22I ATGCAT 2 cut(s) 324, 593
FaeI CATG 1 cut(s) 52
FatI CATG 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 1 cut(s) 192
GluI GCNGC 1 cut(s) 21
HaeIII GGCC 2 cut(s) 149, 501
HapII CCGG 1 cut(s) 452
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 1 cut(s) 52
HindIII AAGCTT 2 cut(s) 343, 506
HinfI GANTC 4 cut(s) 45, 251, 338, 354
HpaII CCGG 1 cut(s) 452
Hpy188I TCNGA 3 cut(s) 44, 129, 177
Hpy188III TCNNGA 4 cut(s) 49, 67, 85, 292
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 3 cut(s) 471, 491, 566
HpyCH4IV ACGT 2 cut(s) 81, 113
HpyCH4V TGCA 5 cut(s) 23, 160, 322, 541, 591
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 461, 575
HpyF3I CTNAG 1 cut(s) 554
HpySE526I ACGT 2 cut(s) 81, 113
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 1 cut(s) 52
LmnI GCTCC 2 cut(s) 368, 575
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 1 cut(s) 578
MaeI CTAG 1 cut(s) 192
MaeII ACGT 2 cut(s) 81, 113
MhlI GDGCHC 1 cut(s) 580
MluCI AATT 4 cut(s) 161, 178, 332, 529
MlyI GAGTC 2 cut(s) 260, 332
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 3 cut(s) 160, 355, 561
Mph1103I ATGCAT 2 cut(s) 324, 593
MroXI GAANNNNTTC 2 cut(s) 447, 532
MseI TTAA 3 cut(s) 257, 299, 384
MslI CAYNNNNRTG 2 cut(s) 116, 491
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 2 cut(s) 220, 525
Mva1269I GAATGC 1 cut(s) 591
MvaI CCWGG 2 cut(s) 220, 525
MwoI GCNNNNNNNGC 3 cut(s) 452, 461, 575
NlaIII CATG 1 cut(s) 52
NmeAIII GCCGAG 1 cut(s) 305
NsiI ATGCAT 2 cut(s) 324, 593
OliI CACNNNNGTG 1 cut(s) 116
PagI TCATGA 1 cut(s) 48
PasI CCCWGGG 1 cut(s) 219
PceI AGGCCT 1 cut(s) 501
PctI GAATGC 1 cut(s) 591
PdmI GAANNNNTTC 2 cut(s) 447, 532
PfeI GAWTC 2 cut(s) 45, 354
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 2 cut(s) 259, 332
PmaCI CACGTG 1 cut(s) 114
PmlI CACGTG 1 cut(s) 114
PpsI GAGTC 2 cut(s) 259, 332
Ppu21I YACGTR 1 cut(s) 114
Psp6I CCWGG 2 cut(s) 218, 523
PspCI CACGTG 1 cut(s) 114
PspGI CCWGG 2 cut(s) 218, 523
PspPI GGNCC 1 cut(s) 147
RsaI GTAC 2 cut(s) 274, 285
RsaNI GTAC 2 cut(s) 273, 284
RseI CAYNNNNRTG 2 cut(s) 116, 491
SaqAI TTAA 3 cut(s) 257, 299, 384
SatI GCNGC 1 cut(s) 21
Sau96I GGNCC 1 cut(s) 147
ScaI AGTACT 2 cut(s) 274, 285
SchI GAGTC 2 cut(s) 260, 332
ScrFI CCNGG 2 cut(s) 220, 525
SduI GDGCHC 1 cut(s) 580
SfaNI GCATC 1 cut(s) 578
SmiMI CAYNNNNRTG 2 cut(s) 116, 491
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
Sse9I AATT 4 cut(s) 161, 178, 332, 529
SseBI AGGCCT 1 cut(s) 501
SspI AATATT 1 cut(s) 262
SspMI CTAG 1 cut(s) 192
StuI AGGCCT 1 cut(s) 501
StyD4I CCNGG 2 cut(s) 218, 523
TaiI ACGT 2 cut(s) 84, 116
TasI AATT 4 cut(s) 161, 178, 332, 529
TatI WGTACW 2 cut(s) 272, 283
TfiI GAWTC 2 cut(s) 45, 354
Tru1I TTAA 3 cut(s) 257, 299, 384
Tru9I TTAA 3 cut(s) 257, 299, 384
TscAI CASTG 1 cut(s) 43
TseI GCWGC 1 cut(s) 20
TspDTI ATGAA 1 cut(s) 456
TspGWI ACGGA 2 cut(s) 374, 407
TspRI CASTG 1 cut(s) 43
XagI CCTNNNNNAGG 1 cut(s) 495
XapI RAATTY 2 cut(s) 178, 332
XmnI GAANNNNTTC 2 cut(s) 447, 532
XspI CTAG 1 cut(s) 192
ZraI GACGTC 1 cut(s) 82
ZrmI AGTACT 2 cut(s) 274, 285
Zsp2I ATGCAT 2 cut(s) 324, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.