Rmu_ssc0000252.1_g000037

Transketolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000252.1
OG0018677
Physical Location & Seq
Reverse (-)
224824 .. 226717
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 441 bp
atggcttcttatctggattattggctggtgaaagcatttctcagactaggtttttgttatggggcaacctcatacagtttccacggcgccacactgagcaccaacgaagttgatgctaccagacaaaaccttggatggccatacgagcatttccacgtgccagagaatgttaaaagtcaccagtgtcaccatactgcagaagtttcagctcttgcagctgaatggaatgccaagtttgctgaatatgagaagaggtatgcagagaaagctgtagagctgaagtccatcatcactggtgagctacccgctggttgggaaaaggcactcctgatcagttctccgcaacattcgattattcatgagaatgttgatctgggattccgaaatcagttctctgcatatgcatttgatccctggcttcatattctgctcaacgtttga

Protein Analysis

146

Amino Acids

16.67

Weight (kDa)

5.95

Isoelectric Point (pI)

28.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transketolase_N PF00456 23 - 102 3.2e-16 Transketolase, thiamine diphosphate binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018677)

Species Orthologous Gene IDs
rosa_multiflora Rmu_ssc0000252.1_g000037
rosa_roxburghii Rroxscaffold_2G00080210 Rroxscaffold_2G00080430
rosa_samantha Rh2CG610700 Rh2CG611900 Rh2DG659000 Rh2DG659600 Rh2DG659900
rosa_wichuraiana Rw2G052270 Rw2G052310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AciI CCGC 2 cut(s) 306, 341
AclI AACGTT 1 cut(s) 435
AclWI GGATC 1 cut(s) 404
AcoI YGGCCR 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 299
AcvI CACGTG 1 cut(s) 157
AcyI GRCGYC 1 cut(s) 87
AfiI CCNNNNNNNGG 1 cut(s) 312
AjnI CCWGG 1 cut(s) 413
AluBI AGCT 5 cut(s) 209, 218, 269, 277, 301
AluI AGCT 5 cut(s) 209, 218, 269, 277, 301
Alw21I GWGCWC 1 cut(s) 101
AlwI GGATC 1 cut(s) 404
AoxI GGCC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 215
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AspLEI GCGC 1 cut(s) 89
AsuHPI GGTGA 4 cut(s) 40, 170, 179, 308
BalI TGGCCA 1 cut(s) 139
BanI GGYRCC 1 cut(s) 86
BbrPI CACGTG 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 227
BccI CCATC 2 cut(s) 129, 293
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 2 cut(s) 95, 129
BciT130I CCWGG 1 cut(s) 415
BclI TGATCA 1 cut(s) 330
BfaI CTAG 1 cut(s) 47
BfmI CTRYAG 2 cut(s) 195, 270
BfoI RGCGCY 1 cut(s) 90
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 415
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 415
BmsI GCATC 1 cut(s) 103
BsaAI YACGTR 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 82, 130, 413
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse1I ACTGG 2 cut(s) 181, 298
BseBI CCWGG 1 cut(s) 415
BseDI CCNNGG 3 cut(s) 82, 130, 413
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMII CTCAG 2 cut(s) 55, 86
BseNI ACTGG 2 cut(s) 181, 298
BseXI GCAGC 1 cut(s) 227
BshFI GGCC 1 cut(s) 139
BshNI GGYRCC 1 cut(s) 86
BsiHKAI GWGCWC 1 cut(s) 101
BslI CCNNNNNNNGG 1 cut(s) 312
BsmI GAATGC 1 cut(s) 232
BsnI GGCC 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 101
Bsp143I GATC 3 cut(s) 330, 370, 409
BspACI CCGC 2 cut(s) 306, 341
BspANI GGCC 1 cut(s) 139
BspCNI CTCAG 2 cut(s) 54, 87
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 88
BspMAI CTGCAG 1 cut(s) 199
BspPI GGATC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 86
BsrI ACTGG 2 cut(s) 181, 298
BssECI CCNNGG 3 cut(s) 82, 130, 413
BssMI GATC 3 cut(s) 330, 370, 409
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 130
Bst2UI CCWGG 1 cut(s) 415
Bst4CI ACNGT 1 cut(s) 77
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 87
BstBAI YACGTR 1 cut(s) 157
BstDEI CTNAG 2 cut(s) 41, 95
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 1 cut(s) 140
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 3 cut(s) 333, 373, 412
BstMBI GATC 3 cut(s) 330, 370, 409
BstMWI GCNNNNNNNGC 4 cut(s) 145, 215, 236, 266
BstNI CCWGG 1 cut(s) 415
BstSCI CCNGG 1 cut(s) 413
BstSFI CTRYAG 2 cut(s) 195, 270
BstV1I GCAGC 1 cut(s) 227
BsuRI GGCC 1 cut(s) 139
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 1 cut(s) 140
BtsIMutI CAGTG 3 cut(s) 92, 188, 291
CciI TCATGA 1 cut(s) 358
CfoI GCGC 1 cut(s) 89
CviAII CATG 1 cut(s) 359
CviJI RGCY 9 cut(s) 5, 25, 139, 209, 218, 269, 277, 301, 418
CviKI_1 RGCY 9 cut(s) 5, 25, 139, 209, 218, 269, 277, 301, 418
DdeI CTNAG 2 cut(s) 41, 95
DinI GGCGCC 1 cut(s) 88
DpnI GATC 3 cut(s) 332, 372, 411
DpnII GATC 3 cut(s) 330, 370, 409
EaeI YGGCCR 1 cut(s) 137
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 299
Eco72I CACGTG 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 413
EcoT14I CCWWGG 1 cut(s) 130
EcoT22I ATGCAT 1 cut(s) 406
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 1 cut(s) 362
FatI CATG 1 cut(s) 358
FauI CCCGC 1 cut(s) 313
FauNDI CATATG 1 cut(s) 400
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 1 cut(s) 47
GlaI GCGC 1 cut(s) 88
GluI GCNGC 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 1 cut(s) 139
HhaI GCGC 1 cut(s) 89
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 1 cut(s) 362
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HinfI GANTC 1 cut(s) 378
HphI GGTGA 4 cut(s) 40, 170, 179, 308
Hpy188I TCNGA 2 cut(s) 44, 383
Hpy188III TCNNGA 3 cut(s) 14, 328, 359
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 2 cut(s) 156, 435
HpyCH4V TGCA 5 cut(s) 197, 215, 260, 398, 404
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 215, 236, 266
HpyF3I CTNAG 2 cut(s) 41, 95
HpySE526I ACGT 2 cut(s) 156, 435
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 1 cut(s) 362
HspAI GCGC 1 cut(s) 87
KasI GGCGCC 1 cut(s) 86
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 3 cut(s) 330, 370, 409
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 1 cut(s) 103
MaeI CTAG 1 cut(s) 47
MaeII ACGT 2 cut(s) 156, 435
MaeIII GTNAC 2 cut(s) 176, 185
MalI GATC 3 cut(s) 332, 372, 411
MboI GATC 3 cut(s) 330, 370, 409
MboII GAAGA 1 cut(s) 262
MhlI GDGCHC 1 cut(s) 101
MlsI TGGCCA 1 cut(s) 139
MluNI TGGCCA 1 cut(s) 139
Mly113I GGCGCC 1 cut(s) 87
MnlI CCTC 2 cut(s) 79, 246
Mox20I TGGCCA 1 cut(s) 139
Mph1103I ATGCAT 1 cut(s) 406
MscI TGGCCA 1 cut(s) 139
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 1 cut(s) 363
Msp20I TGGCCA 1 cut(s) 139
MspA1I CMGCKG 2 cut(s) 218, 308
MspR9I CCNGG 1 cut(s) 415
Mva1269I GAATGC 1 cut(s) 232
MvaI CCWGG 1 cut(s) 415
MwoI GCNNNNNNNGC 4 cut(s) 145, 215, 236, 266
NarI GGCGCC 1 cut(s) 87
NdeI CATATG 1 cut(s) 400
NdeII GATC 3 cut(s) 330, 370, 409
NlaIII CATG 1 cut(s) 362
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 2 cut(s) 176, 185
NsiI ATGCAT 1 cut(s) 406
PagI TCATGA 1 cut(s) 358
PctI GAATGC 1 cut(s) 232
PfeI GAWTC 1 cut(s) 378
PkrI GCNGC 1 cut(s) 217
PluTI GGCGCC 1 cut(s) 90
PmaCI CACGTG 1 cut(s) 157
PmlI CACGTG 1 cut(s) 157
Ppu21I YACGTR 1 cut(s) 157
Psp1406I AACGTT 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 413
PspCI CACGTG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 413
PspN4I GGNNCC 1 cut(s) 88
PstI CTGCAG 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 218
RseI CAYNNNNRTG 1 cut(s) 363
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 3 cut(s) 330, 370, 409
ScrFI CCNGG 1 cut(s) 415
SduI GDGCHC 1 cut(s) 101
SfaNI GCATC 1 cut(s) 103
SfcI CTRYAG 2 cut(s) 195, 270
SfoI GGCGCC 1 cut(s) 88
SmiMI CAYNNNNRTG 1 cut(s) 363
SsiI CCGC 2 cut(s) 306, 341
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 413
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 2 cut(s) 159, 438
TaqI TCGA 1 cut(s) 350
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 3 cut(s) 99, 188, 298
TseFI GTSAC 2 cut(s) 176, 185
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 2 cut(s) 176, 185
TspDTI ATGAA 2 cut(s) 347, 410
TspRI CASTG 3 cut(s) 99, 188, 298
XspI CTAG 1 cut(s) 47
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.