Rh2CG610700

Transketolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0018677
Physical Location & Seq
Reverse (-)
78849891 .. 78857307
7417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG610700.1

Sequence Viewer

Length: 855 bp
ATGGAGGTGAGAGAATGCTTGGAAAAGGCCAGACGAATGACTTCAGGTTTTGAATCAGCAGAAGCCGAACCCAAAGAAGGGGAGGGTGATGAAAATAAGAAAAAATCAATTAAAGGTGGTAAGCAGGATGCTTTAATCAAAAGCGAAAAGGACAAGGCTGTTAGTGCAATAGCGAAAAGTGTACGGCCCGAGGCAACTACTTTTCCTACAGCACCACAAGTCAAACCAGAACATTTCCTACAGCACCCCAAGTCAACCCAGAACTTACTTTGCAGTAATGCAACCTCATACAGTTTCCACGGCGCCACACTGAGCACCAACGAAGTTGATGCTACCAGACAAAGCCTTGGATGGCCATACGAGCATTTCCACGTGCCAAAGAATGTTAAAAGTCACCAGTGTCACCATACTGCAGAAGTTTCAGCTCTTGAAGCTGAATGGAATGCCAAGTTTGCTGAATATGAGAAGAGGTATGCAGAGGAAGCTGTAGAGCTGAAGTCCATCATCACTGGCGAGCTACCCGCTGGTTGGGAAAAGGCACTCCTGACCTACACTCCAGGAGTCCCAGCTGACCGAAGTGTGAAAGGAGAAGAGGTTGAAAAAGAGTGGGAGCGTTTCTCAGTTCGTGTACAGCTGTTCAATTCCTGTGACACCGACTGGTTTCGGCGGTGTGGCACTGCGTCTTTGATCCACATGACAGAGAGTGTGAGAGAGAGTGACAGTCAAGGGTTACTCGATTCTTTCATCCCCAGAAAGAAAGAGAGTGGGAGAGACATAGCGAAGGGAGTAGCGGAAGAAGTTTCAATCTTTGTCATATTGCAAGATCAGCAAGATCCAATAAACTGGTTGAATTGA

Protein Analysis

284

Amino Acids

31.94

Weight (kDa)

5.69

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transketolase_N PF00456 95 - 174 1.1e-15 Transketolase, thiamine diphosphate binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018677)

Species Orthologous Gene IDs
rosa_multiflora Rmu_ssc0000252.1_g000037
rosa_roxburghii Rroxscaffold_2G00080210 Rroxscaffold_2G00080430
rosa_samantha Rh2CG610700 Rh2CG611900 Rh2DG659000 Rh2DG659600 Rh2DG659900
rosa_wichuraiana Rw2G052270 Rw2G052310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 302
AciI CCGC 3 cut(s) 522, 667, 791
AclWI GGATC 2 cut(s) 682, 827
AcoI YGGCCR 1 cut(s) 353
AcuI CTGAAG 2 cut(s) 27, 515
AcvI CACGTG 1 cut(s) 373
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 2 cut(s) 183, 630
AfiI CCNNNNNNNGG 3 cut(s) 77, 78, 528
AgsI TTSAA 6 cut(s) 53, 431, 599, 640, 804, 850
AjnI CCWGG 1 cut(s) 556
AluBI AGCT 7 cut(s) 425, 434, 485, 493, 517, 569, 634
AluI AGCT 7 cut(s) 425, 434, 485, 493, 517, 569, 634
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 1 cut(s) 765
AlwI GGATC 2 cut(s) 682, 827
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 3 cut(s) 27, 185, 353
Asp700I GAANNNNTTC 1 cut(s) 40
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 186
AsuHPI GGTGA 4 cut(s) 19, 98, 386, 395
AvaI CYCGRG 1 cut(s) 188
BalI TGGCCA 1 cut(s) 355
BanI GGYRCC 1 cut(s) 302
BbrPI CACGTG 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 317
BccI CCATC 2 cut(s) 345, 509
BceAI ACGGC 2 cut(s) 200, 316
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 765
BfmI CTRYAG 4 cut(s) 207, 239, 411, 486
BfoI RGCGCY 1 cut(s) 306
Bme1390I CCNGG 1 cut(s) 558
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 186
BmiI GGNNCC 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 558
BmsI GCATC 2 cut(s) 118, 319
BplI GAGNNNNNCTC 2 cut(s) 602, 634
BpmI CTGGAG 1 cut(s) 540
BsaAI YACGTR 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 3 cut(s) 189, 298, 346
BsaXI ACNNNNNCTCC 4 cut(s) 538, 568, 579, 609
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 78, 528
Bse1I ACTGG 4 cut(s) 397, 514, 662, 848
BseBI CCWGG 1 cut(s) 558
BseDI CCNNGG 3 cut(s) 189, 298, 346
BseGI GGATG 3 cut(s) 133, 356, 744
BseLI CCNNNNNNNGG 3 cut(s) 77, 78, 528
BseMII CTCAG 2 cut(s) 302, 633
BseNI ACTGG 4 cut(s) 397, 514, 662, 848
BseYI CCCAGC 1 cut(s) 565
BshFI GGCC 3 cut(s) 29, 187, 355
BshNI GGYRCC 1 cut(s) 302
BsiHKAI GWGCWC 1 cut(s) 317
BsiHKCI CYCGRG 1 cut(s) 188
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 3 cut(s) 77, 78, 528
BsmAI GTCTC 1 cut(s) 765
BsmFI GGGAC 1 cut(s) 548
BsmI GAATGC 2 cut(s) 20, 448
BsnI GGCC 3 cut(s) 29, 187, 355
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 317
Bsp1407I TGTACA 1 cut(s) 628
Bsp143I GATC 3 cut(s) 687, 823, 832
BspACI CCGC 3 cut(s) 522, 667, 791
BspANI GGCC 3 cut(s) 29, 187, 355
BspCNI CTCAG 2 cut(s) 303, 632
BspLI GGNNCC 1 cut(s) 304
BspMAI CTGCAG 1 cut(s) 415
BspPI GGATC 2 cut(s) 682, 827
BspT107I GGYRCC 1 cut(s) 302
BsrGI TGTACA 1 cut(s) 628
BsrI ACTGG 4 cut(s) 397, 514, 662, 848
BssECI CCNNGG 3 cut(s) 189, 298, 346
BssMI GATC 3 cut(s) 687, 823, 832
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 558
Bst4CI ACNGT 2 cut(s) 293, 722
Bst6I CTCTTC 2 cut(s) 461, 585
BstACI GRCGYC 1 cut(s) 303
BstAUI TGTACA 1 cut(s) 628
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 515
BstDEI CTNAG 2 cut(s) 311, 619
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 3 cut(s) 133, 356, 744
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 3 cut(s) 690, 826, 835
BstMAI GTCTC 1 cut(s) 765
BstMBI GATC 3 cut(s) 687, 823, 832
BstMWI GCNNNNNNNGC 6 cut(s) 164, 361, 431, 452, 482, 826
BstNI CCWGG 1 cut(s) 558
BstSCI CCNGG 1 cut(s) 556
BstSFI CTRYAG 4 cut(s) 207, 239, 411, 486
BstX2I RGATCY 1 cut(s) 832
BstXI CCANNNNNNTGG 1 cut(s) 843
BstYI RGATCY 1 cut(s) 832
BsuRI GGCC 3 cut(s) 29, 187, 355
BtgI CCRYGG 1 cut(s) 298
BtsCI GGATG 3 cut(s) 133, 356, 744
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 4 cut(s) 308, 404, 507, 675
Cac8I GCNNGC 1 cut(s) 515
CfoI GCGC 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 186
CseI GACGC 1 cut(s) 669
Csp6I GTAC 2 cut(s) 182, 629
CviAII CATG 1 cut(s) 694
CviQI GTAC 2 cut(s) 182, 629
DdeI CTNAG 2 cut(s) 311, 619
DinI GGCGCC 1 cut(s) 304
DpnI GATC 3 cut(s) 689, 825, 834
DpnII GATC 3 cut(s) 687, 823, 832
EaeI YGGCCR 1 cut(s) 353
Eam1104I CTCTTC 2 cut(s) 461, 585
EarI CTCTTC 2 cut(s) 461, 585
Eco130I CCWWGG 1 cut(s) 346
Eco57I CTGAAG 2 cut(s) 27, 515
Eco72I CACGTG 1 cut(s) 373
Eco88I CYCGRG 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 556
EcoT14I CCWWGG 1 cut(s) 346
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 1 cut(s) 697
FaiI YATR 8 cut(s) 289, 358, 408, 462, 474, 695, 776, 815
FaqI GGGAC 1 cut(s) 548
FatI CATG 1 cut(s) 693
FauI CCCGC 1 cut(s) 529
FokI GGATG 3 cut(s) 140, 363, 731
GlaI GCGC 1 cut(s) 304
GsaI CCCAGC 1 cut(s) 569
GsuI CTGGAG 1 cut(s) 540
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 3 cut(s) 29, 187, 355
HgaI GACGC 1 cut(s) 669
HhaI GCGC 1 cut(s) 305
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 1 cut(s) 697
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HinfI GANTC 3 cut(s) 53, 561, 737
HphI GGTGA 4 cut(s) 19, 98, 386, 395
Hpy166II GTNNAC 3 cut(s) 182, 255, 629
Hpy188III TCNNGA 2 cut(s) 428, 544
Hpy8I GTNNAC 3 cut(s) 182, 255, 629
HpyAV CCTTC 2 cut(s) 71, 775
HpyCH4III ACNGT 2 cut(s) 293, 722
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 6 cut(s) 167, 273, 281, 413, 476, 820
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 361, 431, 452, 482, 826
HpyF3I CTNAG 2 cut(s) 311, 619
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 1 cut(s) 697
HspAI GCGC 1 cut(s) 303
KasI GGCGCC 1 cut(s) 302
Kzo9I GATC 3 cut(s) 687, 823, 832
LmnI GCTCC 1 cut(s) 610
LweI GCATC 2 cut(s) 118, 319
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 5 cut(s) 392, 401, 647, 716, 729
MalI GATC 3 cut(s) 689, 825, 834
MboI GATC 3 cut(s) 687, 823, 832
MboII GAAGA 3 cut(s) 478, 602, 806
MflI RGATCY 1 cut(s) 832
MhlI GDGCHC 1 cut(s) 317
MlsI TGGCCA 1 cut(s) 355
MluCI AATT 3 cut(s) 108, 640, 850
MluNI TGGCCA 1 cut(s) 355
Mly113I GGCGCC 1 cut(s) 303
MlyI GAGTC 1 cut(s) 570
MnlI CCTC 6 cut(s) 76, 184, 295, 462, 472, 586
Mox20I TGGCCA 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 40
MscI TGGCCA 1 cut(s) 355
MseI TTAA 3 cut(s) 111, 134, 387
Msp20I TGGCCA 1 cut(s) 355
MspA1I CMGCKG 3 cut(s) 524, 569, 634
MspR9I CCNGG 1 cut(s) 558
Mva1269I GAATGC 2 cut(s) 20, 448
MvaI CCWGG 1 cut(s) 558
MwoI GCNNNNNNNGC 6 cut(s) 164, 361, 431, 452, 482, 826
NarI GGCGCC 1 cut(s) 303
NdeII GATC 3 cut(s) 687, 823, 832
NlaIII CATG 1 cut(s) 697
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 4 cut(s) 392, 401, 647, 716
PctI GAATGC 2 cut(s) 20, 448
PdmI GAANNNNTTC 1 cut(s) 40
PfeI GAWTC 2 cut(s) 53, 737
PfoI TCCNGGA 1 cut(s) 556
PleI GAGTC 1 cut(s) 569
PluTI GGCGCC 1 cut(s) 306
PmaCI CACGTG 1 cut(s) 373
PmlI CACGTG 1 cut(s) 373
PpsI GAGTC 1 cut(s) 569
Ppu21I YACGTR 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 556
PspCI CACGTG 1 cut(s) 373
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 1 cut(s) 556
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 1 cut(s) 186
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
PstI CTGCAG 1 cut(s) 415
PsuI RGATCY 1 cut(s) 832
PvuII CAGCTG 2 cut(s) 569, 634
RsaI GTAC 2 cut(s) 183, 630
RsaNI GTAC 2 cut(s) 182, 629
SaqAI TTAA 3 cut(s) 111, 134, 387
Sau3AI GATC 3 cut(s) 687, 823, 832
Sau96I GGNCC 1 cut(s) 186
SchI GAGTC 1 cut(s) 570
ScrFI CCNGG 1 cut(s) 558
SduI GDGCHC 1 cut(s) 317
SfaNI GCATC 2 cut(s) 118, 319
SfcI CTRYAG 4 cut(s) 207, 239, 411, 486
SfoI GGCGCC 1 cut(s) 304
Sse9I AATT 3 cut(s) 108, 640, 850
SsiI CCGC 3 cut(s) 522, 667, 791
SspDI GGCGCC 1 cut(s) 302
StyD4I CCNGG 1 cut(s) 556
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 2 cut(s) 293, 722
TaiI ACGT 1 cut(s) 375
TaqI TCGA 1 cut(s) 735
TaqII GACCGA 1 cut(s) 588
TasI AATT 3 cut(s) 108, 640, 850
TatI WGTACW 1 cut(s) 628
TfiI GAWTC 2 cut(s) 53, 737
Tru1I TTAA 3 cut(s) 111, 134, 387
Tru9I TTAA 3 cut(s) 111, 134, 387
TscAI CASTG 4 cut(s) 315, 404, 514, 682
TseFI GTSAC 4 cut(s) 392, 401, 647, 716
Tsp45I GTSAC 4 cut(s) 392, 401, 647, 716
TspDTI ATGAA 2 cut(s) 105, 733
TspRI CASTG 4 cut(s) 315, 404, 514, 682
XmnI GAANNNNTTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.