Rh2DG659000

Transketolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018677
Physical Location & Seq
Reverse (-)
88575973 .. 88578382
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG659000.1

Sequence Viewer

Length: 582 bp
ATGGAGGTGAGAGAATGCTTGGAAAAGGCCAGACGAATGACTTCAGGTTTTGAATCAGCAGAAGCCGAACCCAAAGAAGGGGAGGGTGATGAAAATAAGAAAAAATCAATTAAAGGTGGTAAGCAGGATGCTTTAATCAAAAGCGAAAAGGACAAGGCTGTTAGTGCAATAGCGAAAAGTGTACGGCCCGAGGCAACTACTTTTCCTACAGCACCACAAGTCAAACCAGAACATTTCCTACAGCACCCCAAGTCAACCCAGAACTTACTTTGCAGTAATGCAACCTCATACAGTTTCCACGGCGCCACACTGAGCACCAACGAAGTTGATGCTACCAGACAAAGCCTTGGATGGCCATACGAGCATTTCCACGTGCCAAAGAATGTTAAAAGTCACCAGTGTCACCATACTGCAGAAGTTTCAGCTCTTGAAGCTGAATGGAATGCCAAGTTTGCTGAATATGAGAAGAGGTATGCAGAGGAAGCTGTAGAGCTGAAGTCCATCATCACTGGCGAGCTACCCGCTGGTTGGGAAAAGGCACTCCTGACCTACACTCCAGGAGTCCCAGCTGACCGTGATTGA

Protein Analysis

193

Amino Acids

21.45

Weight (kDa)

6.09

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transketolase_N PF00456 95 - 174 5.1e-16 Transketolase, thiamine diphosphate binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018677)

Species Orthologous Gene IDs
rosa_multiflora Rmu_ssc0000252.1_g000037
rosa_roxburghii Rroxscaffold_2G00080210 Rroxscaffold_2G00080430
rosa_samantha Rh2CG610700 Rh2CG611900 Rh2DG659000 Rh2DG659600 Rh2DG659900
rosa_wichuraiana Rw2G052270 Rw2G052310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 302
AciI CCGC 1 cut(s) 522
AcoI YGGCCR 1 cut(s) 353
AcuI CTGAAG 2 cut(s) 27, 515
AcvI CACGTG 1 cut(s) 373
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 1 cut(s) 183
AfiI CCNNNNNNNGG 3 cut(s) 77, 78, 528
AgsI TTSAA 2 cut(s) 53, 431
AjnI CCWGG 1 cut(s) 556
AluBI AGCT 6 cut(s) 425, 434, 485, 493, 517, 569
AluI AGCT 6 cut(s) 425, 434, 485, 493, 517, 569
Alw21I GWGCWC 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 3 cut(s) 27, 185, 353
Asp700I GAANNNNTTC 1 cut(s) 40
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 186
AsuHPI GGTGA 4 cut(s) 19, 98, 386, 395
AvaI CYCGRG 1 cut(s) 188
BalI TGGCCA 1 cut(s) 355
BanI GGYRCC 1 cut(s) 302
BbrPI CACGTG 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 317
BccI CCATC 2 cut(s) 345, 509
BceAI ACGGC 2 cut(s) 200, 316
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 1 cut(s) 558
BfmI CTRYAG 4 cut(s) 207, 239, 411, 486
BfoI RGCGCY 1 cut(s) 306
Bme1390I CCNGG 1 cut(s) 558
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 186
BmiI GGNNCC 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 558
BmsI GCATC 2 cut(s) 118, 319
BpmI CTGGAG 1 cut(s) 540
BsaAI YACGTR 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 3 cut(s) 189, 298, 346
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 78, 528
Bse1I ACTGG 2 cut(s) 397, 514
BseBI CCWGG 1 cut(s) 558
BseDI CCNNGG 3 cut(s) 189, 298, 346
BseGI GGATG 2 cut(s) 133, 356
BseLI CCNNNNNNNGG 3 cut(s) 77, 78, 528
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 2 cut(s) 397, 514
BseYI CCCAGC 1 cut(s) 565
BshFI GGCC 3 cut(s) 29, 187, 355
BshNI GGYRCC 1 cut(s) 302
BsiHKAI GWGCWC 1 cut(s) 317
BsiHKCI CYCGRG 1 cut(s) 188
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 3 cut(s) 77, 78, 528
BsmFI GGGAC 1 cut(s) 548
BsmI GAATGC 2 cut(s) 20, 448
BsnI GGCC 3 cut(s) 29, 187, 355
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 317
BspACI CCGC 1 cut(s) 522
BspANI GGCC 3 cut(s) 29, 187, 355
BspCNI CTCAG 1 cut(s) 303
BspLI GGNNCC 1 cut(s) 304
BspMAI CTGCAG 1 cut(s) 415
BspT107I GGYRCC 1 cut(s) 302
BsrI ACTGG 2 cut(s) 397, 514
BssECI CCNNGG 3 cut(s) 189, 298, 346
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 558
Bst4CI ACNGT 2 cut(s) 293, 575
Bst6I CTCTTC 1 cut(s) 461
BstACI GRCGYC 1 cut(s) 303
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 515
BstDEI CTNAG 1 cut(s) 311
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 2 cut(s) 133, 356
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 1 cut(s) 305
BstMWI GCNNNNNNNGC 5 cut(s) 164, 361, 431, 452, 482
BstNI CCWGG 1 cut(s) 558
BstSCI CCNGG 1 cut(s) 556
BstSFI CTRYAG 4 cut(s) 207, 239, 411, 486
BsuRI GGCC 3 cut(s) 29, 187, 355
BtgI CCRYGG 1 cut(s) 298
BtsCI GGATG 2 cut(s) 133, 356
BtsIMutI CAGTG 3 cut(s) 308, 404, 507
Cac8I GCNNGC 1 cut(s) 515
CfoI GCGC 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 186
Csp6I GTAC 1 cut(s) 182
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 1 cut(s) 311
DinI GGCGCC 1 cut(s) 304
EaeI YGGCCR 1 cut(s) 353
Eam1104I CTCTTC 1 cut(s) 461
EarI CTCTTC 1 cut(s) 461
Eco130I CCWWGG 1 cut(s) 346
Eco57I CTGAAG 2 cut(s) 27, 515
Eco72I CACGTG 1 cut(s) 373
Eco88I CYCGRG 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 556
EcoT14I CCWWGG 1 cut(s) 346
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 346
FaiI YATR 5 cut(s) 289, 358, 408, 462, 474
FaqI GGGAC 1 cut(s) 548
FauI CCCGC 1 cut(s) 529
FokI GGATG 2 cut(s) 140, 363
GlaI GCGC 1 cut(s) 304
GsaI CCCAGC 1 cut(s) 569
GsuI CTGGAG 1 cut(s) 540
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 3 cut(s) 29, 187, 355
HhaI GCGC 1 cut(s) 305
Hin1I GRCGYC 1 cut(s) 303
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HinfI GANTC 2 cut(s) 53, 561
HphI GGTGA 4 cut(s) 19, 98, 386, 395
Hpy166II GTNNAC 2 cut(s) 182, 255
Hpy188III TCNNGA 2 cut(s) 428, 544
Hpy8I GTNNAC 2 cut(s) 182, 255
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 2 cut(s) 293, 575
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 5 cut(s) 167, 273, 281, 413, 476
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 361, 431, 452, 482
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 303
HspAI GCGC 1 cut(s) 303
KasI GGCGCC 1 cut(s) 302
LweI GCATC 2 cut(s) 118, 319
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 392, 401
MboII GAAGA 1 cut(s) 478
MhlI GDGCHC 1 cut(s) 317
MlsI TGGCCA 1 cut(s) 355
MluCI AATT 1 cut(s) 108
MluNI TGGCCA 1 cut(s) 355
Mly113I GGCGCC 1 cut(s) 303
MlyI GAGTC 1 cut(s) 570
MnlI CCTC 5 cut(s) 76, 184, 295, 462, 472
Mox20I TGGCCA 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 40
MscI TGGCCA 1 cut(s) 355
MseI TTAA 3 cut(s) 111, 134, 387
Msp20I TGGCCA 1 cut(s) 355
MspA1I CMGCKG 2 cut(s) 524, 569
MspR9I CCNGG 1 cut(s) 558
Mva1269I GAATGC 2 cut(s) 20, 448
MvaI CCWGG 1 cut(s) 558
MwoI GCNNNNNNNGC 5 cut(s) 164, 361, 431, 452, 482
NarI GGCGCC 1 cut(s) 303
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 392, 401
PctI GAATGC 2 cut(s) 20, 448
PdmI GAANNNNTTC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 53
PfoI TCCNGGA 1 cut(s) 556
PleI GAGTC 1 cut(s) 569
PluTI GGCGCC 1 cut(s) 306
PmaCI CACGTG 1 cut(s) 373
PmlI CACGTG 1 cut(s) 373
PpsI GAGTC 1 cut(s) 569
Ppu21I YACGTR 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 556
PspCI CACGTG 1 cut(s) 373
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 1 cut(s) 556
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 1 cut(s) 186
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
PstI CTGCAG 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SaqAI TTAA 3 cut(s) 111, 134, 387
Sau96I GGNCC 1 cut(s) 186
SchI GAGTC 1 cut(s) 570
ScrFI CCNGG 1 cut(s) 558
SduI GDGCHC 1 cut(s) 317
SfaNI GCATC 2 cut(s) 118, 319
SfcI CTRYAG 4 cut(s) 207, 239, 411, 486
SfoI GGCGCC 1 cut(s) 304
Sse9I AATT 1 cut(s) 108
SsiI CCGC 1 cut(s) 522
SspDI GGCGCC 1 cut(s) 302
StyD4I CCNGG 1 cut(s) 556
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 2 cut(s) 293, 575
TaiI ACGT 1 cut(s) 375
TasI AATT 1 cut(s) 108
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 3 cut(s) 111, 134, 387
Tru9I TTAA 3 cut(s) 111, 134, 387
TscAI CASTG 3 cut(s) 315, 404, 514
TseFI GTSAC 2 cut(s) 392, 401
Tsp45I GTSAC 2 cut(s) 392, 401
TspDTI ATGAA 1 cut(s) 105
TspRI CASTG 3 cut(s) 315, 404, 514
XmnI GAANNNNTTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.