Rmu_ssc0000396.1_g000008

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000396.1
OG0019002
Physical Location & Seq
Reverse (-)
56841 .. 63541
6701 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2382 bp
atgtttctgatgcctaacccaattgatgacatcatcatcctcccgccacatgaccatccatgcttatcattatggcacagacttctgaacttgaacttcattatccaaaacataaacaagatcttcttcacaagtaatattgtaccagaaatcccttcctctatctcgtctctctcattttgtaacatccagaagtccactcctctctgtctcgtctttctgattttgtctctgtcaattgtagcgagaaacatttttcaggactctgaaatggtgaaggcacaaggagcgagcgatcacctcgtgaaaattttccctgaaaatatgcaggacattccacaggatgagaactccaaagatcagtttctaattcaatccaagtcagggggtacccaggttttatggaataatgtgcagaaattgaaatctgagcccgtgtacaagaattctttcgtgaccgaaagcttatggtcccctttagggacttatttggcaaatgttaagaaacaggatatcattctttttagaggcacctctgccttacatcatgtggccacctgtctgcatcccaaggtttttgaggtgaaatgggttgatttttcgcctggtgagaattatttggtgtcctacagtgttgaacaaaaaaacactggtgaaataaccagagctgcaataagcatttttgatgtgaaaactggaaaagttgtgaaaaaaattgtgggaggtcctcatgattttgcaaataaaggagttggggcgtcttggcccattttcaaatggtccggtggaagagatgacaagtattttgccctaatcagccagaatgttatctctatttatgagataaatacatttacagccatattgacggtagggaatgtcatggacttcagttggtcaccaactgatgcgattcttgctctctttgtatcagaatcggttgatgctggtggtgccatacctgctttggtgaaactggttcaaatacctagtaaagagcagttgattgagaagaagctttctagtgttagtaagtgcaagatgtactggcaaagcaatggggagtatcttgctatgaaagttgacagcagtacaaacaatttcgttgagcttttccaaattaaagatcgaggcatcgataataaaattttggcgctcccgtataataaggacaaggtcactgcttttgcttgggaacccatgggccataggtttgctattattcagagtaatgaagatattagtttctactccatgcgggatgctaccagcacaggctgtgtttcattgctcacgattctcagaagcaaggggacaaactatctcttttggtcaccttctggtggcctcataatacttgcaaggttgaatggttccaatgggaatttggaatttttcaatgtggatgaacttcggacagttgctatatctaaacatatagcaacggctcttgagtggaacccaaccgggagatatgttgctacttctactcctgggacttgtcatgagaagccgagttttaacatttggtcttttgatggccacctactctaccaattacagaaggatctcttcacaaagttttcatggcgaccaaggccactcaacatggcgactaaggctattatggggtccatgagccataacagcttgcattcactcaacatggaagatgatgagtattcactcaacatggaagatgatgagtattcactcaacatggaagatgatgagtattcactcaacatggaagatgatgagtattcactcaacatggaagatgatgataattccatgagtttgactgggggtatcaagaagtctgtggctttcatggaaaagtttcacgaagatttggatgccttttataggctggatgatgaggaaaggcagttattgatagacctcaagtacctcactttgagccccgcttctcaaaaacctaaggagaaagatgtagtcatagttatcagtgatgatgaggaggaggaaaagcatgtattgatgaaccttaaataccttgcttcgagctccacttctaaaaaacctgagggggcagatgtagtcataggtatcagtgacgaggaggaaaagatggctttgcagccatcttctccaaagaaaacacttccaatggctgggcaaaccaaggaaagacggttccatagaaaatggaccaaagaagaagaagaggctttaaggcatggttataaaaagtataaaacaaattggaaattgattttgggtgatccagcctacaaagcttgccatggcagaactaatgttgatttgaaagacaaatggcggaatttgatgagggctgatgacagggctaaggcgaaggcctgctactcattaccaaatggaattgatggtacgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

793

Amino Acids

89.73

Weight (kDa)

5.82

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 338 - 525 2.8e-31 Eukaryotic translation initiation factor eIF2A
Beta-prop_WDR19_1st PF23389 348 - 480 5.3e-06 WDR19 first beta-propeller
Beta-prop_THOC3 PF25174 363 - 497 2.2e-07 THOC3 beta-propeller domain
Myb_DNA-binding PF00249 723 - 771 5.7e-07 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 725 - 775 4.5e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019002)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0447321
rosa_laevigata RLG00000026012
rosa_multiflora Rmu_ssc0000396.1_g000008
rosa_roxburghii Rroxscaffold_5G00387700
rosa_samantha Rh4AG441000 Rh4BG450300 Rh4CG467400 Rh4DG448700
rosa_wichuraiana Rw4G037750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2211
Acc36I ACCTGC 1 cut(s) 970
Acc65I GGTACC 1 cut(s) 389
AccB1I GGYRCC 3 cut(s) 389, 530, 953
AccB7I CCANNNNNTGG 1 cut(s) 2138
AciI CCGC 4 cut(s) 44, 1258, 1929, 2305
AclWI GGATC 2 cut(s) 1575, 2243
AcoI YGGCCR 2 cut(s) 552, 1540
AcsI RAATTY 6 cut(s) 309, 445, 1146, 1384, 1391, 2308
AcuI CTGAAG 1 cut(s) 874
AcyI GRCGYC 1 cut(s) 758
AdeI CACNNNGTG 1 cut(s) 304
AfaI GTAC 7 cut(s) 144, 391, 440, 1046, 1093, 1913, 2377
AfiI CCNNNNNNNGG 5 cut(s) 384, 480, 1343, 1869, 2138
AgsI TTSAA 9 cut(s) 94, 374, 424, 638, 775, 983, 1369, 1399, 2293
AjnI CCWGG 3 cut(s) 393, 604, 1492
AjuI GAANNNNNNNTTGG 2 cut(s) 2225, 2257
AloI GAACNNNNNNTCC 2 cut(s) 2144, 2176
AluBI AGCT 7 cut(s) 465, 668, 1018, 1111, 1650, 2031, 2264
AluI AGCT 7 cut(s) 465, 668, 1018, 1111, 1650, 2031, 2264
Alw21I GWGCWC 1 cut(s) 2033
Alw26I GTCTC 3 cut(s) 174, 215, 234
AlwI GGATC 2 cut(s) 1575, 2243
AoxI GGCC 7 cut(s) 552, 764, 1204, 1345, 1540, 1598, 2343
ApeKI GCWGC 2 cut(s) 668, 2104
ApoI RAATTY 6 cut(s) 309, 445, 1146, 1384, 1391, 2308
ArsI GACNNNNNNTTYG 2 cut(s) 788, 820
Asp700I GAANNNNTTC 3 cut(s) 311, 449, 1842
Asp718I GGTACC 1 cut(s) 389
AspLEI GCGC 1 cut(s) 1156
AspS9I GGNCC 7 cut(s) 471, 725, 765, 780, 1204, 1632, 2175
AsuC2I CCSGG 1 cut(s) 1468
AsuHPI GGTGA 9 cut(s) 286, 290, 595, 620, 665, 891, 982, 1326, 2258
AvaII GGWCC 5 cut(s) 471, 725, 780, 1632, 2175
AxyI CCTNAGG 2 cut(s) 1944, 2049
BaeI ACNNNNGTAYC 2 cut(s) 381, 414
BalI TGGCCA 2 cut(s) 554, 1542
BanI GGYRCC 3 cut(s) 389, 530, 953
BanII GRGCYC 3 cut(s) 435, 1928, 2033
BarI GAAGNNNNNNTAC 2 cut(s) 2333, 2365
BauI CACGAG 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 2033
BbvI GCAGC 2 cut(s) 655, 2116
BccI CCATC 5 cut(s) 63, 1532, 2089, 2116, 2366
BceAI ACGGC 1 cut(s) 1461
BciT130I CCWGG 3 cut(s) 395, 606, 1494
BcnI CCSGG 1 cut(s) 1468
BcoDI GTCTC 3 cut(s) 174, 215, 234
BfaI CTAG 2 cut(s) 990, 1023
BfmI CTRYAG 1 cut(s) 628
BfoI RGCGCY 1 cut(s) 1157
BfuAI ACCTGC 1 cut(s) 970
BglII AGATCT 1 cut(s) 120
BisI GCNGC 2 cut(s) 669, 2105
BlsI GCNGC 2 cut(s) 670, 2106
Bme1390I CCNGG 4 cut(s) 395, 606, 1468, 1494
Bme18I GGWCC 5 cut(s) 471, 725, 780, 1632, 2175
BmgT120I GGNCC 7 cut(s) 471, 725, 765, 780, 1204, 1632, 2175
BmiI GGNNCC 9 cut(s) 391, 473, 532, 955, 1197, 1375, 1460, 1633, 2162
BmrFI CCNGG 4 cut(s) 395, 606, 1468, 1494
BmrI ACTGGG 1 cut(s) 1815
BmsI GCATC 6 cut(s) 574, 898, 934, 1143, 1252, 1849
BmuI ACTGGG 1 cut(s) 1815
Bpu10I CCTNAGC 1 cut(s) 2334
BpuEI CTTGAG 2 cut(s) 1472, 1892
BpuMI CCSGG 1 cut(s) 1468
Bsa29I ATCGAT 1 cut(s) 1137
BsaAI YACGTR 1 cut(s) 2379
BsaHI GRCGYC 1 cut(s) 758
BsaJI CCNNGG 7 cut(s) 393, 570, 1200, 1493, 1595, 2148, 2269
BsaWI WCCGGW 1 cut(s) 782
BsaXI ACNNNNNCTCC 2 cut(s) 1474, 1504
Bsc4I CCNNNNNNNGG 5 cut(s) 384, 480, 1343, 1869, 2138
Bse1I ACTGG 5 cut(s) 655, 700, 981, 1052, 1810
Bse21I CCTNAGG 2 cut(s) 1944, 2049
Bse3DI GCAATG 2 cut(s) 1063, 1286
BseBI CCWGG 3 cut(s) 395, 606, 1494
BseCI ATCGAT 1 cut(s) 1137
BseDI CCNNGG 7 cut(s) 393, 570, 1200, 1493, 1595, 2148, 2269
BseGI GGATG 9 cut(s) 36, 55, 186, 349, 565, 1267, 1411, 1864, 1882
BseLI CCNNNNNNNGG 5 cut(s) 384, 480, 1343, 1869, 2138
BseMI GCAATG 2 cut(s) 1063, 1286
BseMII CTCAG 3 cut(s) 420, 1315, 2040
BseNI ACTGG 5 cut(s) 655, 700, 981, 1052, 1810
BseRI GAGGAG 4 cut(s) 192, 1997, 2000, 2099
BseXI GCAGC 2 cut(s) 655, 2116
BseYI CCCAGC 1 cut(s) 2138
BsgI GTGCAG 1 cut(s) 434
BshFI GGCC 7 cut(s) 554, 766, 1206, 1347, 1542, 1600, 2345
BshNI GGYRCC 3 cut(s) 389, 530, 953
BshVI ATCGAT 1 cut(s) 1137
BsiHKAI GWGCWC 1 cut(s) 2033
BsiSI CCGG 2 cut(s) 783, 1467
BslFI GGGAC 4 cut(s) 457, 496, 1327, 1510
BslI CCNNNNNNNGG 5 cut(s) 384, 480, 1343, 1869, 2138
BsmAI GTCTC 3 cut(s) 174, 215, 234
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 4 cut(s) 457, 496, 1327, 1510
BsmI GAATGC 1 cut(s) 1654
BsnI GGCC 7 cut(s) 554, 766, 1206, 1347, 1542, 1600, 2345
Bsp1286I GDGCHC 3 cut(s) 435, 1928, 2033
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 6 cut(s) 120, 295, 358, 1126, 1567, 2248
Bsp19I CCATGG 2 cut(s) 1200, 2269
BspACI CCGC 4 cut(s) 44, 1258, 1929, 2305
BspANI GGCC 7 cut(s) 554, 766, 1206, 1347, 1542, 1600, 2345
BspCNI CTCAG 3 cut(s) 421, 1314, 2041
BspDI ATCGAT 1 cut(s) 1137
BspHI TCATGA 2 cut(s) 730, 1504
BspLI GGNNCC 9 cut(s) 391, 473, 532, 955, 1197, 1375, 1460, 1633, 2162
BspMI ACCTGC 1 cut(s) 970
BspPI GGATC 2 cut(s) 1575, 2243
BspT107I GGYRCC 3 cut(s) 389, 530, 953
BsrDI GCAATG 2 cut(s) 1063, 1286
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 5 cut(s) 655, 700, 981, 1052, 1810
BssECI CCNNGG 7 cut(s) 393, 570, 1200, 1493, 1595, 2148, 2269
BssMI GATC 6 cut(s) 120, 295, 358, 1126, 1567, 2248
BssNI GRCGYC 1 cut(s) 758
BssSI CACGAG 1 cut(s) 302
BssT1I CCWWGG 5 cut(s) 570, 1200, 1595, 2148, 2269
Bst2BI CACGAG 1 cut(s) 302
Bst2UI CCWGG 3 cut(s) 395, 606, 1494
Bst4CI ACNGT 4 cut(s) 632, 871, 1420, 2160
Bst6I CTCTTC 3 cut(s) 784, 1577, 2184
BstACI GRCGYC 1 cut(s) 758
BstAUI TGTACA 1 cut(s) 438
BstBAI YACGTR 1 cut(s) 2379
BstC8I GCNNGC 4 cut(s) 292, 1652, 2266, 2347
BstDEI CTNAG 6 cut(s) 429, 1301, 1617, 1944, 2049, 2334
BstDSI CCRYGG 2 cut(s) 1200, 2269
BstEII GGTNACC 2 cut(s) 897, 1332
BstENI CCTNNNNNAGG 1 cut(s) 1867
BstF5I GGATG 9 cut(s) 36, 55, 186, 349, 565, 1267, 1411, 1864, 1882
BstH2I RGCGCY 1 cut(s) 1157
BstHHI GCGC 1 cut(s) 1156
BstKTI GATC 6 cut(s) 123, 298, 361, 1129, 1570, 2251
BstMAI GTCTC 3 cut(s) 174, 215, 234
BstMBI GATC 6 cut(s) 120, 295, 358, 1126, 1567, 2248
BstMWI GCNNNNNNNGC 8 cut(s) 287, 917, 953, 962, 1597, 1619, 1647, 2261
BstNI CCWGG 3 cut(s) 395, 606, 1494
BstNSI RCATGY 1 cut(s) 2000
BstPI GGTNACC 2 cut(s) 897, 1332
BstSCI CCNGG 4 cut(s) 393, 604, 1466, 1492
BstSFI CTRYAG 1 cut(s) 628
BstV1I GCAGC 2 cut(s) 655, 2116
BstX2I RGATCY 2 cut(s) 120, 1567
BstYI RGATCY 2 cut(s) 120, 1567
Bsu15I ATCGAT 1 cut(s) 1137
Bsu36I CCTNAGG 2 cut(s) 1944, 2049
BsuRI GGCC 7 cut(s) 554, 766, 1206, 1347, 1542, 1600, 2345
BsuTUI ATCGAT 1 cut(s) 1137
BtgI CCRYGG 2 cut(s) 1200, 2269
BtsCI GGATG 9 cut(s) 36, 55, 186, 349, 565, 1267, 1411, 1864, 1882
BtsI GCAGTG 1 cut(s) 1179
BtsIMutI CAGTG 5 cut(s) 637, 648, 1179, 1978, 2083
BveI ACCTGC 1 cut(s) 970
Cac8I GCNNGC 4 cut(s) 292, 1652, 2266, 2347
CciI TCATGA 2 cut(s) 730, 1504
CfoI GCGC 1 cut(s) 1156
Cfr13I GGNCC 7 cut(s) 471, 725, 765, 780, 1204, 1632, 2175
ClaI ATCGAT 1 cut(s) 1137
CseI GACGC 1 cut(s) 747
Csp6I GTAC 7 cut(s) 143, 390, 439, 1045, 1092, 1912, 2376
CviQI GTAC 7 cut(s) 143, 390, 439, 1045, 1092, 1912, 2376
DdeI CTNAG 6 cut(s) 429, 1301, 1617, 1944, 2049, 2334
DpnI GATC 6 cut(s) 122, 297, 360, 1128, 1569, 2250
DpnII GATC 6 cut(s) 120, 295, 358, 1126, 1567, 2248
DraIII CACNNNGTG 1 cut(s) 304
EaeI YGGCCR 2 cut(s) 552, 1540
Eam1104I CTCTTC 3 cut(s) 784, 1577, 2184
EarI CTCTTC 3 cut(s) 784, 1577, 2184
EciI GGCGGA 1 cut(s) 2320
Ecl136II GAGCTC 1 cut(s) 2031
Eco130I CCWWGG 5 cut(s) 570, 1200, 1595, 2148, 2269
Eco147I AGGCCT 1 cut(s) 2345
Eco24I GRGCYC 3 cut(s) 435, 1928, 2033
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 5 cut(s) 471, 725, 780, 1632, 2175
Eco53kI GAGCTC 1 cut(s) 2031
Eco57I CTGAAG 1 cut(s) 874
Eco81I CCTNAGG 2 cut(s) 1944, 2049
Eco91I GGTNACC 2 cut(s) 897, 1332
EcoICRI GAGCTC 1 cut(s) 2031
EcoNI CCTNNNNNAGG 1 cut(s) 1867
EcoO109I RGGNCCY 1 cut(s) 725
EcoO65I GGTNACC 2 cut(s) 897, 1332
EcoRI GAATTC 1 cut(s) 445
EcoRII CCWGG 3 cut(s) 393, 604, 1492
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 5 cut(s) 570, 1200, 1595, 2148, 2269
EcoT38I GRGCYC 3 cut(s) 435, 1928, 2033
ErhI CCWWGG 5 cut(s) 570, 1200, 1595, 2148, 2269
Esp3I CGTCTC 1 cut(s) 174
FalI AAGNNNNNCTT 4 cut(s) 110, 142, 1556, 1588
FaqI GGGAC 4 cut(s) 457, 496, 1327, 1510
FauI CCCGC 3 cut(s) 51, 1251, 1936
Fnu4HI GCNGC 2 cut(s) 669, 2105
FokI GGATG 9 cut(s) 23, 42, 173, 356, 552, 1274, 1418, 1871, 1889
FriOI GRGCYC 3 cut(s) 435, 1928, 2033
Fsp4HI GCNGC 2 cut(s) 669, 2105
FspBI CTAG 2 cut(s) 990, 1023
GlaI GCGC 1 cut(s) 1155
GluI GCNGC 2 cut(s) 669, 2105
GsaI CCCAGC 1 cut(s) 2142
HaeII RGCGCY 1 cut(s) 1157
HaeIII GGCC 7 cut(s) 554, 766, 1206, 1347, 1542, 1600, 2345
HapII CCGG 2 cut(s) 783, 1467
HgaI GACGC 1 cut(s) 747
HhaI GCGC 1 cut(s) 1156
Hin1I GRCGYC 1 cut(s) 758
Hin6I GCGC 1 cut(s) 1154
HinP1I GCGC 1 cut(s) 1154
HincII GTYRAC 1 cut(s) 1084
HindII GTYRAC 1 cut(s) 1084
HindIII AAGCTT 3 cut(s) 463, 1016, 2262
HinfI GANTC 4 cut(s) 263, 913, 935, 1297
HpaII CCGG 2 cut(s) 783, 1467
HphI GGTGA 9 cut(s) 286, 290, 595, 620, 665, 891, 982, 1326, 2258
Hpy166II GTNNAC 3 cut(s) 198, 439, 1084
Hpy188I TCNGA 9 cut(s) 9, 87, 222, 268, 430, 934, 1227, 1304, 1416
Hpy8I GTNNAC 3 cut(s) 198, 439, 1084
HpyAV CCTTC 5 cut(s) 165, 271, 1347, 1558, 2335
HpyCH4III ACNGT 4 cut(s) 632, 871, 1420, 2160
HpyCH4IV ACGT 1 cut(s) 2378
HpyCH4V TGCA 9 cut(s) 328, 415, 565, 671, 740, 1038, 1361, 1654, 2104
HpyF10VI GCNNNNNNNGC 8 cut(s) 287, 917, 953, 962, 1597, 1619, 1647, 2261
HpyF3I CTNAG 6 cut(s) 429, 1301, 1617, 1944, 2049, 2334
HpySE526I ACGT 1 cut(s) 2378
Hsp92I GRCGYC 1 cut(s) 758
HspAI GCGC 1 cut(s) 1154
KpnI GGTACC 1 cut(s) 393
Kzo9I GATC 6 cut(s) 120, 295, 358, 1126, 1567, 2248
LmnI GCTCC 3 cut(s) 287, 1161, 2036
Lsp1109I GCAGC 2 cut(s) 655, 2116
LweI GCATC 6 cut(s) 574, 898, 934, 1143, 1252, 1849
MaeI CTAG 2 cut(s) 990, 1023
MaeII ACGT 1 cut(s) 2378
MaeIII GTNAC 6 cut(s) 182, 454, 897, 1177, 1332, 2078
MalI GATC 6 cut(s) 122, 297, 360, 1128, 1569, 2250
MboI GATC 6 cut(s) 120, 295, 358, 1126, 1567, 2248
MfeI CAATTG 2 cut(s) 21, 237
MflI RGATCY 2 cut(s) 120, 1567
MhlI GDGCHC 3 cut(s) 435, 1928, 2033
MlsI TGGCCA 2 cut(s) 554, 1542
MluNI TGGCCA 2 cut(s) 554, 1542
MlyI GAGTC 1 cut(s) 257
Mox20I TGGCCA 2 cut(s) 554, 1542
MroXI GAANNNNTTC 3 cut(s) 311, 449, 1842
MscI TGGCCA 2 cut(s) 554, 1542
MseI TTAA 5 cut(s) 501, 1122, 1521, 2013, 2198
Msp20I TGGCCA 2 cut(s) 554, 1542
MspI CCGG 2 cut(s) 783, 1467
MspR9I CCNGG 4 cut(s) 395, 606, 1468, 1494
MunI CAATTG 2 cut(s) 21, 237
Mva1269I GAATGC 1 cut(s) 1654
MvaI CCWGG 3 cut(s) 395, 606, 1494
MwoI GCNNNNNNNGC 8 cut(s) 287, 917, 953, 962, 1597, 1619, 1647, 2261
NciI CCSGG 1 cut(s) 1468
NcoI CCATGG 2 cut(s) 1200, 2269
NdeII GATC 6 cut(s) 120, 295, 358, 1126, 1567, 2248
NlaIV GGNNCC 9 cut(s) 391, 473, 532, 955, 1197, 1375, 1460, 1633, 2162
NmeAIII GCCGAG 1 cut(s) 1539
NmuCI GTSAC 5 cut(s) 454, 897, 1177, 1332, 2078
NspI RCATGY 1 cut(s) 2000
PagI TCATGA 2 cut(s) 730, 1504
PceI AGGCCT 1 cut(s) 2345
PctI GAATGC 1 cut(s) 1654
PdmI GAANNNNTTC 3 cut(s) 311, 449, 1842
PfeI GAWTC 3 cut(s) 913, 935, 1297
PflFI GACNNNGTC 1 cut(s) 1175
PflMI CCANNNNNTGG 1 cut(s) 2138
PkrI GCNGC 2 cut(s) 670, 2106
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 2379
PpuMI RGGWCCY 1 cut(s) 725
PsiI TTATAA 1 cut(s) 2211
Psp124BI GAGCTC 1 cut(s) 2033
Psp5II RGGWCCY 1 cut(s) 725
Psp6I CCWGG 3 cut(s) 393, 604, 1492
PspEI GGTNACC 2 cut(s) 897, 1332
PspFI CCCAGC 1 cut(s) 2138
PspGI CCWGG 3 cut(s) 393, 604, 1492
PspN4I GGNNCC 9 cut(s) 391, 473, 532, 955, 1197, 1375, 1460, 1633, 2162
PspPI GGNCC 7 cut(s) 471, 725, 765, 780, 1204, 1632, 2175
PspPPI RGGWCCY 1 cut(s) 725
PsrI GAACNNNNNNTAC 2 cut(s) 2000, 2032
PsuI RGATCY 2 cut(s) 120, 1567
PsyI GACNNNGTC 1 cut(s) 1175
RsaI GTAC 7 cut(s) 144, 391, 440, 1046, 1093, 1913, 2377
RsaNI GTAC 7 cut(s) 143, 390, 439, 1045, 1092, 1912, 2376
SacI GAGCTC 1 cut(s) 2033
SaqAI TTAA 5 cut(s) 501, 1122, 1521, 2013, 2198
SatI GCNGC 2 cut(s) 669, 2105
Sau3AI GATC 6 cut(s) 120, 295, 358, 1126, 1567, 2248
Sau96I GGNCC 7 cut(s) 471, 725, 765, 780, 1204, 1632, 2175
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 4 cut(s) 395, 606, 1468, 1494
SduI GDGCHC 3 cut(s) 435, 1928, 2033
SfaNI GCATC 6 cut(s) 574, 898, 934, 1143, 1252, 1849
SfcI CTRYAG 1 cut(s) 628
SinI GGWCC 5 cut(s) 471, 725, 780, 1632, 2175
SmlI CTYRAG 2 cut(s) 1451, 1907
SmoI CTYRAG 2 cut(s) 1451, 1907
SseBI AGGCCT 1 cut(s) 2345
SsiI CCGC 4 cut(s) 44, 1258, 1929, 2305
SspI AATATT 1 cut(s) 139
SspMI CTAG 2 cut(s) 990, 1023
SstI GAGCTC 1 cut(s) 2033
StuI AGGCCT 1 cut(s) 2345
StyD4I CCNGG 4 cut(s) 393, 604, 1466, 1492
StyI CCWWGG 5 cut(s) 570, 1200, 1595, 2148, 2269
TaaI ACNGT 4 cut(s) 632, 871, 1420, 2160
TaiI ACGT 1 cut(s) 2381
TaqI TCGA 3 cut(s) 1129, 1137, 2027
TaqII GACCGA 1 cut(s) 473
TatI WGTACW 3 cut(s) 438, 1044, 1091
TfiI GAWTC 3 cut(s) 913, 935, 1297
Tru1I TTAA 5 cut(s) 501, 1122, 1521, 2013, 2198
Tru9I TTAA 5 cut(s) 501, 1122, 1521, 2013, 2198
TscAI CASTG 5 cut(s) 637, 655, 1186, 1978, 2083
TseFI GTSAC 5 cut(s) 454, 897, 1177, 1332, 2078
TseI GCWGC 2 cut(s) 668, 2104
Tsp45I GTSAC 5 cut(s) 454, 897, 1177, 1332, 2078
TspDTI ATGAA 8 cut(s) 88, 1091, 1248, 1275, 1422, 1575, 1822, 2021
TspRI CASTG 5 cut(s) 637, 655, 1186, 1978, 2083
Tth111I GACNNNGTC 1 cut(s) 1175
Van91I CCANNNNNTGG 1 cut(s) 2138
VpaK11BI GGWCC 5 cut(s) 471, 725, 780, 1632, 2175
XagI CCTNNNNNAGG 1 cut(s) 1867
XapI RAATTY 6 cut(s) 309, 445, 1146, 1384, 1391, 2308
XceI RCATGY 1 cut(s) 2000
XcmI CCANNNNNNNNNTGG 2 cut(s) 964, 1384
XmnI GAANNNNTTC 3 cut(s) 311, 449, 1842
XspI CTAG 2 cut(s) 990, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.