Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000456.1

Orthogroup ID

OG0006348

Physical Location & Seq

Genomic Coordinates Reverse (-)

100162 .. 102515

Gene Structure 2354 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 678 bp

atggaaaaagcaaaggatgctaacattgttgcaatcttagtaggaacccttggtgtagctggttacttgaatatgattcatcagatgaaagaactaattacagcagctggaaaaaaagtttatactctagttatgggaagaccgaaccctgcaaaacttgccaactttccagagtgtgatattttcatttatgtctcttgcgcccaaactgctctgctggatagcaaagaatatttagctccagtaatcactccttttgaagcgatgctagctttcagcagaggatctcagtggaccggagcttatgtaatggaattccgcgatttgattaattcttctccagtggactcgagaaaccagtctgaagaagcacgattttccttcatacaaggtggatatgtagaagagtttgatcgggaagggaatgatgctgatgaagacaaggaaggagctcttgctttagcaaatgcgacagagaaggctctgcaattgcgtgacaaccctaattccatagccaagcgaacggctaagtctggtgcagagtttttagcagctcgatcctatcagggtctagatatacattatgacacttccttgccaggaccatatctaatggggaggagtgggagggcagcagggtatcaagatgaaaaaagcatgcaagagaaattggaatga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006417 GO:0006448 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0010608 GO:0017182 GO:0017183 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0032268 GO:0034248 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:1900247 GO:1901564 GO:2000112 GO:2000765

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Diphthamide_syn

Protein Analysis

225

Amino Acids

24.74

Weight (kDa)

5.04

Isoelectric Point (pI)

42.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Diphthamide_syn	PF01866	1 - 102	4.3e-33	Putative diphthamide synthesis protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006348)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59630 AT3G59630 AT3G59630
fragaria_vesca	FvH4_4g10580 FvH4_4g10580 FvH4_4g10580 FvH4_4g10580 FvH4_4g10580 FvH4_4g10580 FvH4_4g10580 FvH4_4g10580 FvH4_4g10580
malus_domestica	MD04G1048400.v1.1
prunus_persica	Prupe.1G080200_v2.0.a1
pyrus_communis	pycom04g04270
rosa_chinensis	RchiOBHm_Chr4g0406211
rosa_laevigata	RLG00000008789
rosa_multiflora	Rmu_sc0012210.1_g000001 Rmu_ssc0000456.1_g000017 Rmu_ssc0000456.1_g000024
rosa_roxburghii	Rroxscaffold_5G00350920
rosa_rugosa	Rorug04G0066000
rosa_samantha	Rh4AG136300 Rh4BG133700 Rh4CG144500 Rh4DG131200
rosa_wichuraiana	Rw4G011180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	321
AciI	CCGC	1 cut(s)	319
AclWI	GGATC	2 cut(s)	292, 552
AcsI	RAATTY	1 cut(s)	314
AcuI	CTGAAG	1 cut(s)	384
AgsI	TTSAA	2 cut(s)	70, 260
AjnI	CCWGG	1 cut(s)	598
AluBI	AGCT	7 cut(s)	59, 107, 239, 272, 302, 452, 554
AluI	AGCT	7 cut(s)	59, 107, 239, 272, 302, 452, 554
Alw21I	GWGCWC	1 cut(s)	454
Alw26I	GTCTC	1 cut(s)	199
AlwI	GGATC	2 cut(s)	292, 552
AlwNI	CAGNNNCTG	1 cut(s)	107
Ama87I	CYCGRG	1 cut(s)	349
ApeKI	GCWGC	3 cut(s)	104, 551, 632
ApoI	RAATTY	1 cut(s)	314
AseI	ATTAAT	1 cut(s)	330
AspLEI	GCGC	1 cut(s)	203
AspS9I	GGNCC	2 cut(s)	294, 602
AsuNHI	GCTAGC	1 cut(s)	268
AvaI	CYCGRG	1 cut(s)	349
AvaII	GGWCC	2 cut(s)	294, 602
BanII	GRGCYC	1 cut(s)	454
BbsI	GAAGAC	2 cut(s)	145, 444
Bbv12I	GWGCWC	1 cut(s)	454
BbvI	GCAGC	3 cut(s)	116, 563, 644
BceAI	ACGGC	1 cut(s)	540
BciT130I	CCWGG	1 cut(s)	600
BcoDI	GTCTC	1 cut(s)	199
BfaI	CTAG	3 cut(s)	128, 269, 572
BisI	GCNGC	3 cut(s)	105, 552, 633
BlsI	GCNGC	3 cut(s)	106, 553, 634
Bme1390I	CCNGG	1 cut(s)	600
Bme18I	GGWCC	2 cut(s)	294, 602
BmeT110I	CYCGRG	1 cut(s)	349
BmgT120I	GGNCC	2 cut(s)	294, 602
BmiI	GGNNCC	1 cut(s)	46
BmrFI	CCNGG	1 cut(s)	600
BmsI	GCATC	3 cut(s)	7, 255, 418
BmtI	GCTAGC	1 cut(s)	272
BpiI	GAAGAC	2 cut(s)	145, 444
BpmI	CTGGAG	2 cut(s)	225, 324
BsaJI	CCNNGG	1 cut(s)	49
BsaWI	WCCGGW	1 cut(s)	296
BsaXI	ACNNNNNCTCC	2 cut(s)	291, 321
Bse1I	ACTGG	3 cut(s)	242, 341, 358
BseBI	CCWGG	1 cut(s)	600
BseDI	CCNNGG	1 cut(s)	49
BseGI	GGATG	1 cut(s)	22
BseMII	CTCAG	1 cut(s)	302
BseNI	ACTGG	3 cut(s)	242, 341, 358
BseRI	GAGGAG	1 cut(s)	634
BseXI	GCAGC	3 cut(s)	116, 563, 644
BsgI	GTGCAG	1 cut(s)	558
Bsh1236I	CGCG	1 cut(s)	321
BsiHKAI	GWGCWC	1 cut(s)	454
BsiHKCI	CYCGRG	1 cut(s)	349
BsiSI	CCGG	1 cut(s)	297
BsmAI	GTCTC	1 cut(s)	199
BsoBI	CYCGRG	1 cut(s)	349
Bsp1286I	GDGCHC	1 cut(s)	454
Bsp143I	GATC	3 cut(s)	284, 412, 557
BspACI	CCGC	1 cut(s)	319
BspCNI	CTCAG	1 cut(s)	301
BspFNI	CGCG	1 cut(s)	321
BspLI	GGNNCC	1 cut(s)	46
BspOI	GCTAGC	1 cut(s)	272
BspPI	GGATC	2 cut(s)	292, 552
BsrI	ACTGG	3 cut(s)	242, 341, 358
BssECI	CCNNGG	1 cut(s)	49
BssMI	GATC	3 cut(s)	284, 412, 557
BssT1I	CCWWGG	1 cut(s)	49
Bst2UI	CCWGG	1 cut(s)	600
Bst6I	CTCTTC	1 cut(s)	399
BstAPI	GCANNNNNTGC	2 cut(s)	17, 158
BstC8I	GCNNGC	2 cut(s)	270, 659
BstDEI	CTNAG	3 cut(s)	37, 288, 528
BstF5I	GGATG	1 cut(s)	22
BstFNI	CGCG	1 cut(s)	321
BstHHI	GCGC	1 cut(s)	203
BstKTI	GATC	3 cut(s)	287, 415, 560
BstMAI	GTCTC	1 cut(s)	199
BstMBI	GATC	3 cut(s)	284, 412, 557
BstMWI	GCNNNNNNNGC	4 cut(s)	17, 158, 209, 269
BstNI	CCWGG	1 cut(s)	600
BstNSI	RCATGY	1 cut(s)	661
BstSCI	CCNGG	1 cut(s)	598
BstUI	CGCG	1 cut(s)	321
BstV1I	GCAGC	3 cut(s)	116, 563, 644
BstV2I	GAAGAC	2 cut(s)	145, 444
BstX2I	RGATCY	1 cut(s)	284
BstYI	RGATCY	1 cut(s)	284
BtgZI	GCGATG	1 cut(s)	278
BtsCI	GGATG	1 cut(s)	22
BtsIMutI	CAGTG	2 cut(s)	296, 348
Cac8I	GCNNGC	2 cut(s)	270, 659
CaiI	CAGNNNCTG	1 cut(s)	107
CfoI	GCGC	1 cut(s)	203
Cfr13I	GGNCC	2 cut(s)	294, 602
CviAII	CATG	1 cut(s)	658
DdeI	CTNAG	3 cut(s)	37, 288, 528
DpnI	GATC	3 cut(s)	286, 414, 559
DpnII	GATC	3 cut(s)	284, 412, 557
Eam1104I	CTCTTC	1 cut(s)	399
EarI	CTCTTC	1 cut(s)	399
Ecl136II	GAGCTC	1 cut(s)	452
Eco130I	CCWWGG	1 cut(s)	49
Eco24I	GRGCYC	1 cut(s)	454
Eco47I	GGWCC	2 cut(s)	294, 602
Eco53kI	GAGCTC	1 cut(s)	452
Eco57I	CTGAAG	1 cut(s)	384
Eco88I	CYCGRG	1 cut(s)	349
EcoICRI	GAGCTC	1 cut(s)	452
EcoRI	GAATTC	1 cut(s)	314
EcoRII	CCWGG	1 cut(s)	598
EcoT14I	CCWWGG	1 cut(s)	49
EcoT38I	GRGCYC	1 cut(s)	454
ErhI	CCWWGG	1 cut(s)	49
FaeI	CATG	1 cut(s)	661
FalI	AAGNNNNNCTT	2 cut(s)	438, 470
FatI	CATG	1 cut(s)	657
Fnu4HI	GCNGC	3 cut(s)	105, 552, 633
FokI	GGATG	1 cut(s)	29
FriOI	GRGCYC	1 cut(s)	454
Fsp4HI	GCNGC	3 cut(s)	105, 552, 633
FspBI	CTAG	3 cut(s)	128, 269, 572
GlaI	GCGC	1 cut(s)	202
GluI	GCNGC	3 cut(s)	105, 552, 633
GsuI	CTGGAG	2 cut(s)	225, 324
HapII	CCGG	1 cut(s)	297
HhaI	GCGC	1 cut(s)	203
Hin1II	CATG	1 cut(s)	661
Hin6I	GCGC	1 cut(s)	201
HinP1I	GCGC	1 cut(s)	201
HinfI	GANTC	2 cut(s)	76, 347
HpaII	CCGG	1 cut(s)	297
Hpy166II	GTNNAC	2 cut(s)	294, 346
Hpy188I	TCNGA	2 cut(s)	84, 364
Hpy188III	TCNNGA	5 cut(s)	170, 351, 416, 572, 644
Hpy8I	GTNNAC	2 cut(s)	294, 346
HpyAV	CCTTC	4 cut(s)	391, 413, 440, 472
HpyCH4V	TGCA	5 cut(s)	32, 152, 487, 539, 661
HpyF10VI	GCNNNNNNNGC	4 cut(s)	17, 158, 209, 269
HpyF3I	CTNAG	3 cut(s)	37, 288, 528
Hsp92II	CATG	1 cut(s)	661
HspAI	GCGC	1 cut(s)	201
Kzo9I	GATC	3 cut(s)	284, 412, 557
LmnI	GCTCC	3 cut(s)	244, 299, 449
Lsp1109I	GCAGC	3 cut(s)	116, 563, 644
LweI	GCATC	3 cut(s)	7, 255, 418
MaeI	CTAG	3 cut(s)	128, 269, 572
MaeIII	GTNAC	2 cut(s)	62, 494
MalI	GATC	3 cut(s)	286, 414, 559
MboI	GATC	3 cut(s)	284, 412, 557
MboII	GAAGA	5 cut(s)	150, 327, 377, 416, 449
MfeI	CAATTG	1 cut(s)	488
MflI	RGATCY	1 cut(s)	284
MhlI	GDGCHC	1 cut(s)	454
MluCI	AATT	6 cut(s)	96, 314, 331, 488, 505, 668
MlyI	GAGTC	1 cut(s)	341
MnlI	CCTC	3 cut(s)	275, 612, 621
MseI	TTAA	1 cut(s)	330
MspA1I	CMGCKG	1 cut(s)	107
MspI	CCGG	1 cut(s)	297
MspR9I	CCNGG	1 cut(s)	600
MunI	CAATTG	1 cut(s)	488
MvaI	CCWGG	1 cut(s)	600
MvnI	CGCG	1 cut(s)	321
MwoI	GCNNNNNNNGC	4 cut(s)	17, 158, 209, 269
NdeII	GATC	3 cut(s)	284, 412, 557
NheI	GCTAGC	1 cut(s)	268
NlaIII	CATG	1 cut(s)	661
NlaIV	GGNNCC	1 cut(s)	46
NmuCI	GTSAC	1 cut(s)	494
NspI	RCATGY	1 cut(s)	661
PaeI	GCATGC	1 cut(s)	661
PaeR7I	CTCGAG	1 cut(s)	349
PfeI	GAWTC	1 cut(s)	76
PkrI	GCNGC	3 cut(s)	106, 553, 634
PleI	GAGTC	1 cut(s)	341
PpsI	GAGTC	1 cut(s)	341
PshBI	ATTAAT	1 cut(s)	330
Psp124BI	GAGCTC	1 cut(s)	454
Psp6I	CCWGG	1 cut(s)	598
PspGI	CCWGG	1 cut(s)	598
PspN4I	GGNNCC	1 cut(s)	46
PspPI	GGNCC	2 cut(s)	294, 602
PstNI	CAGNNNCTG	1 cut(s)	107
PsuI	RGATCY	1 cut(s)	284
PvuII	CAGCTG	1 cut(s)	107
SacI	GAGCTC	1 cut(s)	454
SaqAI	TTAA	1 cut(s)	330
SatI	GCNGC	3 cut(s)	105, 552, 633
Sau3AI	GATC	3 cut(s)	284, 412, 557
Sau96I	GGNCC	2 cut(s)	294, 602
SchI	GAGTC	1 cut(s)	341
ScrFI	CCNGG	1 cut(s)	600
SduI	GDGCHC	1 cut(s)	454
SetI	ASST	8 cut(s)	61, 109, 241, 274, 304, 394, 454, 556
SfaNI	GCATC	3 cut(s)	7, 255, 418
Sfr274I	CTCGAG	1 cut(s)	349
SinI	GGWCC	2 cut(s)	294, 602
SlaI	CTCGAG	1 cut(s)	349
SmlI	CTYRAG	1 cut(s)	349
SmoI	CTYRAG	1 cut(s)	349
SphI	GCATGC	1 cut(s)	661
Sse9I	AATT	6 cut(s)	96, 314, 331, 488, 505, 668
SsiI	CCGC	1 cut(s)	319
SspI	AATATT	1 cut(s)	233
SspMI	CTAG	3 cut(s)	128, 269, 572
SstI	GAGCTC	1 cut(s)	454
StyD4I	CCNGG	1 cut(s)	598
StyI	CCWWGG	1 cut(s)	49
TaqI	TCGA	2 cut(s)	350, 556
TaqII	GACCGA	1 cut(s)	157
TasI	AATT	6 cut(s)	96, 314, 331, 488, 505, 668
TfiI	GAWTC	1 cut(s)	76
Tru1I	TTAA	1 cut(s)	330
Tru9I	TTAA	1 cut(s)	330
TscAI	CASTG	2 cut(s)	296, 348
TseFI	GTSAC	1 cut(s)	494
TseI	GCWGC	3 cut(s)	104, 551, 632
Tsp45I	GTSAC	1 cut(s)	494
TspDTI	ATGAA	6 cut(s)	68, 101, 175, 373, 450, 663
TspRI	CASTG	2 cut(s)	296, 348
VpaK11BI	GGWCC	2 cut(s)	294, 602
VspI	ATTAAT	1 cut(s)	330
XapI	RAATTY	1 cut(s)	314
XbaI	TCTAGA	1 cut(s)	571
XceI	RCATGY	1 cut(s)	661
XhoI	CTCGAG	1 cut(s)	349
XspI	CTAG	3 cut(s)	128, 269, 572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.