Rmu_ssc0000473.1_g000025
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000473.1
OG0019448
Physical Location & Seq
Reverse (-)
145068 .. 146502
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
atggatggcacccatattccagcgatggtaaaaggtcgcaaagtaagcagctatcgtaatcatcatggaattcagtctcaaaatgttttgtcagcttgcgactttgatttacaattcatatatgtgataagtgggtgggaaggttcggcacatgattcaaaattgttaaataatgccttatcaaggagaaatggacttgaagttcctcaggattcagaggacccatatgattccaatttcaaattggaattacagaggctgtggtgttttgggctcccgaatccttttcgccagcagcttataagtttgaggaggagtggtgaggttgtcagagctcatgatcttccgaagctcggcctggaccttgaagaatacttgtcgccagctgcccctgttgtcggaaccgagaatcttggcccaggtaatggtcggggaggagatgggctggaacttgagggagagggacaagagccttcgctagagagccgaagcctagttgaggcaggagccctgggagacgggtggggcgccctggtttgcgggcattctcttctggaggctctgaacaatggagcttatgatgaagatggaggtggaggagaagagagagaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

204

Amino Acids

22.08

Weight (kDa)

4.73

Isoelectric Point (pI)

56.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 1 - 59 3.1e-07 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019448)

Species Orthologous Gene IDs
rosa_laevigata RLG00000001334 RLG00000002601 RLG00000003708 RLG00000005142 RLG00000020279
rosa_multiflora Rmu_ssc0000473.1_g000025
rosa_roxburghii Rroxscaffold_4G00320620
rosa_samantha Rh4DG215400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 302
AccB1I GGYRCC 2 cut(s) 8, 527
AccB7I CCANNNNNTGG 1 cut(s) 425
AciI CCGC 1 cut(s) 540
AcsI RAATTY 1 cut(s) 69
AcyI GRCGYC 1 cut(s) 528
AfiI CCNNNNNNNGG 5 cut(s) 183, 353, 398, 425, 499
AgsI TTSAA 4 cut(s) 159, 200, 241, 368
AjnI CCWGG 4 cut(s) 357, 418, 510, 531
AloI GAACNNNNNNTCC 2 cut(s) 186, 218
AluBI AGCT 7 cut(s) 51, 95, 298, 335, 352, 386, 575
AluI AGCT 7 cut(s) 51, 95, 298, 335, 352, 386, 575
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 2 cut(s) 81, 510
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 2 cut(s) 355, 415
ApeKI GCWGC 3 cut(s) 48, 295, 386
ApoI RAATTY 1 cut(s) 69
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 3 cut(s) 220, 361, 416
AsuHPI GGTGA 1 cut(s) 332
AvaII GGWCC 2 cut(s) 220, 361
AxyI CCTNAGG 1 cut(s) 207
BanI GGYRCC 2 cut(s) 8, 527
BanII GRGCYC 3 cut(s) 276, 337, 511
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 3 cut(s) 60, 307, 373
BccI CCATC 3 cut(s) 19, 434, 581
BciT130I CCWGG 4 cut(s) 359, 420, 512, 533
BcoDI GTCTC 2 cut(s) 81, 510
BfaI CTAG 2 cut(s) 479, 494
BfoI RGCGCY 1 cut(s) 531
BisI GCNGC 3 cut(s) 49, 296, 387
BlsI GCNGC 3 cut(s) 50, 297, 388
Bme1390I CCNGG 4 cut(s) 359, 420, 512, 533
Bme18I GGWCC 2 cut(s) 220, 361
BmgT120I GGNCC 3 cut(s) 220, 361, 416
BmiI GGNNCC 6 cut(s) 10, 222, 275, 403, 508, 529
BmrFI CCNGG 4 cut(s) 359, 420, 512, 533
BpmI CTGGAG 1 cut(s) 575
BpuEI CTTGAG 1 cut(s) 473
BsaHI GRCGYC 1 cut(s) 528
BsaJI CCNNGG 4 cut(s) 418, 510, 511, 531
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 5 cut(s) 183, 353, 398, 425, 499
Bse21I CCTNAGG 1 cut(s) 207
BseBI CCWGG 4 cut(s) 359, 420, 512, 533
BseDI CCNNGG 4 cut(s) 418, 510, 511, 531
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 5 cut(s) 183, 353, 398, 425, 499
BseMII CTCAG 1 cut(s) 221
BseRI GAGGAG 4 cut(s) 325, 328, 450, 612
BseXI GCAGC 3 cut(s) 60, 307, 373
BshFI GGCC 2 cut(s) 357, 417
BshNI GGYRCC 2 cut(s) 8, 527
BsiHKAI GWGCWC 1 cut(s) 337
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 5 cut(s) 183, 353, 398, 425, 499
BsmAI GTCTC 2 cut(s) 81, 510
BsmBI CGTCTC 1 cut(s) 510
BsmFI GGGAC 1 cut(s) 477
BsmI GAATGC 1 cut(s) 544
BsnI GGCC 2 cut(s) 357, 417
Bsp1286I GDGCHC 3 cut(s) 276, 337, 511
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 1 cut(s) 540
BspANI GGCC 2 cut(s) 357, 417
BspCNI CTCAG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 6 cut(s) 10, 222, 275, 403, 508, 529
BspT107I GGYRCC 2 cut(s) 8, 527
BssECI CCNNGG 4 cut(s) 418, 510, 511, 531
BssMI GATC 1 cut(s) 340
BssNI GRCGYC 1 cut(s) 528
Bst2UI CCWGG 4 cut(s) 359, 420, 512, 533
Bst6I CTCTTC 2 cut(s) 555, 597
BstACI GRCGYC 1 cut(s) 528
BstC8I GCNNGC 4 cut(s) 97, 293, 384, 542
BstDEI CTNAG 1 cut(s) 207
BstENI CCTNNNNNAGG 2 cut(s) 181, 497
BstF5I GGATG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 2 cut(s) 81, 510
BstMBI GATC 1 cut(s) 340
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNI CCWGG 4 cut(s) 359, 420, 512, 533
BstSCI CCNGG 4 cut(s) 357, 418, 510, 531
BstV1I GCAGC 3 cut(s) 60, 307, 373
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 2 cut(s) 357, 417
BtgZI GCGATG 1 cut(s) 38
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 4 cut(s) 97, 293, 384, 542
CaiI CAGNNNCTG 1 cut(s) 259
CciI TCATGA 1 cut(s) 337
CfoI GCGC 1 cut(s) 530
Cfr13I GGNCC 3 cut(s) 220, 361, 416
CviAII CATG 3 cut(s) 65, 152, 338
DdeI CTNAG 1 cut(s) 207
DinI GGCGCC 1 cut(s) 529
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
Eam1104I CTCTTC 2 cut(s) 555, 597
EarI CTCTTC 2 cut(s) 555, 597
Ecl136II GAGCTC 1 cut(s) 335
Eco24I GRGCYC 3 cut(s) 276, 337, 511
Eco47I GGWCC 2 cut(s) 220, 361
Eco53kI GAGCTC 1 cut(s) 335
Eco81I CCTNAGG 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 335
EcoNI CCTNNNNNAGG 2 cut(s) 181, 497
EcoO109I RGGNCCY 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 69
EcoRII CCWGG 4 cut(s) 357, 418, 510, 531
EcoT38I GRGCYC 3 cut(s) 276, 337, 511
EgeI GGCGCC 1 cut(s) 529
EheI GGCGCC 1 cut(s) 529
Esp3I CGTCTC 1 cut(s) 510
FaeI CATG 3 cut(s) 68, 155, 341
FaqI GGGAC 1 cut(s) 477
FatI CATG 3 cut(s) 64, 151, 337
FauI CCCGC 1 cut(s) 533
FauNDI CATATG 1 cut(s) 226
Fnu4HI GCNGC 3 cut(s) 49, 296, 387
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 3 cut(s) 276, 337, 511
Fsp4HI GCNGC 3 cut(s) 49, 296, 387
FspBI CTAG 2 cut(s) 479, 494
GlaI GCGC 1 cut(s) 529
GluI GCNGC 3 cut(s) 49, 296, 387
GsuI CTGGAG 1 cut(s) 575
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 2 cut(s) 357, 417
HhaI GCGC 1 cut(s) 530
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 3 cut(s) 68, 155, 341
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HinfI GANTC 5 cut(s) 155, 212, 230, 280, 409
HphI GGTGA 1 cut(s) 332
Hpy188I TCNGA 5 cut(s) 217, 332, 348, 401, 564
Hpy188III TCNNGA 4 cut(s) 209, 277, 338, 554
HpyAV CCTTC 2 cut(s) 134, 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 3 cut(s) 68, 155, 341
HspAI GCGC 1 cut(s) 528
KasI GGCGCC 1 cut(s) 527
Kzo9I GATC 1 cut(s) 340
LmnI GCTCC 3 cut(s) 279, 506, 572
Lsp1109I GCAGC 3 cut(s) 60, 307, 373
MaeI CTAG 2 cut(s) 479, 494
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 5 cut(s) 335, 380, 542, 596, 614
MhlI GDGCHC 3 cut(s) 276, 337, 511
MluCI AATT 6 cut(s) 69, 113, 161, 235, 242, 248
Mly113I GGCGCC 1 cut(s) 528
MmeI TCCRAC 1 cut(s) 379
MseI TTAA 1 cut(s) 167
MslI CAYNNNNRTG 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 386
MspR9I CCNGG 4 cut(s) 359, 420, 512, 533
Mva1269I GAATGC 1 cut(s) 544
MvaI CCWGG 4 cut(s) 359, 420, 512, 533
MwoI GCNNNNNNNGC 1 cut(s) 45
NarI GGCGCC 1 cut(s) 528
NdeI CATATG 1 cut(s) 226
NdeII GATC 1 cut(s) 340
NlaIII CATG 3 cut(s) 68, 155, 341
NlaIV GGNNCC 6 cut(s) 10, 222, 275, 403, 508, 529
NmeAIII GCCGAG 1 cut(s) 333
PagI TCATGA 1 cut(s) 337
PasI CCCWGGG 1 cut(s) 511
PctI GAATGC 1 cut(s) 544
PfeI GAWTC 5 cut(s) 155, 212, 230, 280, 409
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 3 cut(s) 50, 297, 388
PluTI GGCGCC 1 cut(s) 531
PpuMI RGGWCCY 1 cut(s) 220
PsiI TTATAA 1 cut(s) 302
Psp124BI GAGCTC 1 cut(s) 337
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 4 cut(s) 357, 418, 510, 531
PspGI CCWGG 4 cut(s) 357, 418, 510, 531
PspN4I GGNNCC 6 cut(s) 10, 222, 275, 403, 508, 529
PspPI GGNCC 3 cut(s) 220, 361, 416
PspPPI RGGWCCY 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 259
PvuII CAGCTG 1 cut(s) 386
RseI CAYNNNNRTG 1 cut(s) 122
SacI GAGCTC 1 cut(s) 337
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 3 cut(s) 49, 296, 387
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 3 cut(s) 220, 361, 416
ScrFI CCNGG 4 cut(s) 359, 420, 512, 533
SduI GDGCHC 3 cut(s) 276, 337, 511
SfoI GGCGCC 1 cut(s) 529
SinI GGWCC 2 cut(s) 220, 361
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 6 cut(s) 69, 113, 161, 235, 242, 248
SsiI CCGC 1 cut(s) 540
SspDI GGCGCC 1 cut(s) 527
SspMI CTAG 2 cut(s) 479, 494
SstI GAGCTC 1 cut(s) 337
StyD4I CCNGG 4 cut(s) 357, 418, 510, 531
TasI AATT 6 cut(s) 69, 113, 161, 235, 242, 248
TfiI GAWTC 5 cut(s) 155, 212, 230, 280, 409
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TseI GCWGC 3 cut(s) 48, 295, 386
TspDTI ATGAA 2 cut(s) 106, 597
Van91I CCANNNNNTGG 1 cut(s) 425
VpaK11BI GGWCC 2 cut(s) 220, 361
XagI CCTNNNNNAGG 2 cut(s) 181, 497
XapI RAATTY 1 cut(s) 69
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XspI CTAG 2 cut(s) 479, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.