Rroxscaffold_1G00021150

Protein disulfide isomerase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010087
Physical Location & Seq
Reverse (-)
25919068 .. 25925252
6185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00021150.1

Sequence Viewer

Length: 1620 bp
ATGAATGGTCAACAAAAGTTGGGGATTTTAGACACAGCAAAACCCCGCGCTACAACTTTTCCGCAACTCCAAGAAACGACGTCGCATAGAGTCATTGACTCCTCGTCCTCCATTTCTATTAAACCCCGACTAAAACCAGATCTCAGTCTCTCAAGTCTCTCTGCTCGAGCATTCTTCTCCTCACTCACTCCAAAATGGCGAGTCGACTCGTTCTCGTGTTCACACTCAGCGCCCTGCTCCTCCTCTCCCTCCCGCCCAGCTCTCTCCAAGGACGCCGCCGTCGACGACGACGAGGAGGATCTCAGCTTCCTGGAAGAGCCGACCGAGCAGCAGGGCCACGGCCACGACGCCGCGCCGCACTATCCAGATCCGGACGAATTCGACGCCGATAACTACGACGACGCCGACGGCTTCTCCGATTTCGACGAGGGCGATCAGGAGGCGTACAAGGAGCCCGAGGTCGACGAGAAGGACGTCGTCGTTTTGAAGGGGGCGAACTTCAGCGAGACGATTAAGAAGAACAGGTTCGTGCTGGTTGAGTTCTACGCGCCGTGGTGCGGACACTGCCAGGCCTTGGCTCCGGAGTACGCGGCGGCCGCGACGGAGCTCAAGGGCGGAGATGTAGTTTTGGCCAAAATTGATGCGACGGAGGAGAACGAGTTGTCTCAGGAGTATGATGTGCAGGGTTTCCCGACTATCTTCTTCTTCAGCGACGGTGTTCACAAGCCTTATACAGGGCAAAGGACCAAAGAGGCTATTGTTACCTGGATCAAGAAGAAGACAGGACCTGGTATCTCTAACGTGACCACAGTGGAGGATGCAGAGCGCATTTTGACTTCTGAAACTAAATTCGTTTTGGGATTCCTCAACTCTTTGGTGGGTCCTGAGAGTGATGAGCTTGCTGCTGCTTCAAGACTTGAGGATGATGTCAACTTCTACCAAACAGTGGATCCAAAGGTGGCAAAACTTTTCCATATTGACTCTGAGGCCAAACGCCCAGCGCTGGTCCTGATAAAGAAAGAGGATGAGAAGCTGAGCTATTTTGCCGGTAAATTCGACAAGTCTGCAATTGTGGAGTTTGTCTTTGCAAACAAGCTTCCTTTGGTTGTCACATTTACTAGGGAGAATGCCCCTTCCATTTTTGAAAGTGAAATAAAGAAACAGCTGTTGCTCTTTGCCACTTCAACCGACTCAAAGAAGGTTCTCCCAGAGTTTGTAGAGGCAGCAAAGTTATTCAAAGGAAAGGCTTTTGGGGAGGACTTCCTTGAGGATAAACTTAAAGCTTTCTATAAGTCAGACCCGATCCCGGAGACTAATGATGGAGATGTGAAAATAGTGGTTGGAAATAACTTTGATGATATTGTCTTGGATGAGTCGAAGGATGTTCTTCTCGAGGCTGATGGGTTCCCCACACTTCTATTCTTCCCAGCTGGAAACAAGAGCTTTGATCCTATTACAGTAGATGCTGACCGTACAGTGGTGGCGTTCTACAAGTTCCTCAAGAAACATGCATCAATCCCTTTCAAGCTCCAAAAGCCAACTTCAACTACAAAATCCGAGGAATCTGATGCTAAAGAGAGCCAGGAGAGCAGCAAAAACGATGTGAAGGATGAATTGTGA

Protein Analysis

539

Amino Acids

59.76

Weight (kDa)

4.71

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 159 - 259 1.1e-31 Thioredoxin
Thioredoxin_2 PF13098 172 - 253 2.5e-06 Thioredoxin-like domain
Thioredoxin_7 PF13899 172 - 236 2e-06 Thioredoxin-like
Thioredoxin_6 PF13848 300 - 415 3.4e-15 Thioredoxin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54960 AT3G54960 AT5G60640 AT5G60640 AT5G60640
fragaria_vesca FvH4_3g32410
malus_domestica MD03G1117500.v1.1 MD11G1135800.v1.1 MD13G1134800.v1.1
prunus_persica Prupe.6G101200_v2.0.a1
pyrus_communis pycom11g11260
rosa_chinensis RchiOBHm_Chr5g0059381
rosa_laevigata RLG00000035286
rosa_multiflora Rmu_sc0000375.1_g000007
rosa_roxburghii Rroxscaffold_1G00021150
rosa_rugosa Rorug05G0322600
rosa_samantha Rh5AG387100 Rh5BG398400 Rh5CG422400 Rh5DG414200
rosa_wichuraiana Rw5G036420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AatII GACGTC 2 cut(s) 83, 477
AccB7I CCANNNNNTGG 2 cut(s) 574, 946
AccI GTMKAC 3 cut(s) 204, 282, 462
AccII CGCG 5 cut(s) 48, 353, 548, 590, 599
AccIII TCCGGA 2 cut(s) 370, 580
AclWI GGATC 7 cut(s) 306, 362, 776, 944, 957, 1297, 1442
AcoI YGGCCR 3 cut(s) 340, 594, 630
AcsI RAATTY 3 cut(s) 377, 848, 1052
AcuI CTGAAG 2 cut(s) 484, 691
AcyI GRCGYC 6 cut(s) 80, 273, 348, 384, 402, 474
AfaI GTAC 3 cut(s) 446, 587, 1474
AfeI AGCGCT 1 cut(s) 1002
AfiI CCNNNNNNNGG 7 cut(s) 557, 574, 734, 946, 1003, 1306, 1477
AgsI TTSAA 7 cut(s) 487, 912, 1145, 1185, 1237, 1525, 1545
AhdI GACNNNNNGTC 1 cut(s) 103
AjnI CCWGG 5 cut(s) 309, 567, 764, 787, 1581
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 5 cut(s) 152, 161, 500, 669, 1304
AlwI GGATC 7 cut(s) 306, 362, 776, 944, 957, 1297, 1442
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 3 cut(s) 165, 455, 1391
Aor13HI TCCGGA 2 cut(s) 370, 580
Aor51HI AGCGCT 1 cut(s) 1002
AoxI GGCC 6 cut(s) 334, 340, 570, 594, 630, 987
ApeKI GCWGC 5 cut(s) 328, 902, 905, 1223, 1590
ApoI RAATTY 3 cut(s) 377, 848, 1052
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 6 cut(s) 50, 232, 355, 550, 828, 1003
AspS9I GGNCC 5 cut(s) 334, 744, 785, 881, 1006
AsuC2I CCSGG 1 cut(s) 1307
AvaI CYCGRG 3 cut(s) 165, 455, 1391
AvaII GGWCC 4 cut(s) 744, 785, 881, 1006
BalI TGGCCA 1 cut(s) 632
BamHI GGATCC 1 cut(s) 949
BanII GRGCYC 2 cut(s) 456, 609
BauI CACGAG 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 785
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 5 cut(s) 340, 889, 892, 1235, 1602
BccI CCATC 2 cut(s) 1313, 1394
BceAI ACGGC 4 cut(s) 263, 355, 424, 535
BcgI CGANNNNNNTGC 2 cut(s) 1046, 1080
BciT130I CCWGG 5 cut(s) 311, 569, 766, 789, 1583
BcnI CCSGG 1 cut(s) 1307
BcoDI GTCTC 5 cut(s) 152, 161, 500, 669, 1304
BfaI CTAG 1 cut(s) 1119
BfoI RGCGCY 2 cut(s) 233, 1004
BglII AGATCT 1 cut(s) 139
BlpI GCTNAGC 1 cut(s) 1034
Bme1390I CCNGG 6 cut(s) 311, 569, 766, 789, 1307, 1583
Bme18I GGWCC 4 cut(s) 744, 785, 881, 1006
BmeRI GACNNNNNGTC 1 cut(s) 103
BmeT110I CYCGRG 3 cut(s) 165, 455, 1391
BmgT120I GGNCC 5 cut(s) 334, 744, 785, 881, 1006
BmiI GGNNCC 5 cut(s) 453, 579, 882, 951, 1406
BmrFI CCNGG 6 cut(s) 311, 569, 766, 789, 1307, 1583
BmsI GCATC 5 cut(s) 631, 808, 1453, 1520, 1558
BpiI GAAGAC 1 cut(s) 785
Bpu1102I GCTNAGC 1 cut(s) 1034
BpuEI CTTGAG 5 cut(s) 136, 593, 938, 1286, 1484
BpuMI CCSGG 1 cut(s) 1307
BsaHI GRCGYC 6 cut(s) 80, 273, 348, 384, 402, 474
BsaJI CCNNGG 6 cut(s) 267, 337, 456, 551, 573, 1557
BsaWI WCCGGW 2 cut(s) 370, 580
BsaXI ACNNNNNCTCC 6 cut(s) 398, 428, 609, 639, 644, 674
Bsc4I CCNNNNNNNGG 7 cut(s) 557, 574, 734, 946, 1003, 1306, 1477
Bse118I RCCGGY 1 cut(s) 1046
BseAI TCCGGA 2 cut(s) 370, 580
BseBI CCWGG 5 cut(s) 311, 569, 766, 789, 1583
BseDI CCNNGG 6 cut(s) 267, 337, 456, 551, 573, 1557
BseGI GGATG 6 cut(s) 823, 928, 1030, 1375, 1387, 1615
BseLI CCNNNNNNNGG 7 cut(s) 557, 574, 734, 946, 1003, 1306, 1477
BseMII CTCAG 7 cut(s) 157, 240, 316, 680, 876, 975, 1025
BseRI GAGGAG 6 cut(s) 91, 169, 229, 232, 308, 665
BseX3I CGGCCG 1 cut(s) 594
BseXI GCAGC 5 cut(s) 340, 889, 892, 1235, 1602
BseYI CCCAGC 3 cut(s) 256, 997, 1426
BsgI GTGCAG 1 cut(s) 701
Bsh1236I CGCG 5 cut(s) 48, 353, 548, 590, 599
Bsh1285I CGRYCG 2 cut(s) 324, 597
BshFI GGCC 6 cut(s) 336, 342, 572, 596, 632, 989
BsiEI CGRYCG 2 cut(s) 324, 597
BsiHKAI GWGCWC 1 cut(s) 609
BsiHKCI CYCGRG 3 cut(s) 165, 455, 1391
BsiSI CCGG 4 cut(s) 371, 581, 1047, 1307
BslI CCNNNNNNNGG 7 cut(s) 557, 574, 734, 946, 1003, 1306, 1477
BsmAI GTCTC 5 cut(s) 152, 161, 500, 669, 1304
BsmBI CGTCTC 1 cut(s) 500
BsmI GAATGC 2 cut(s) 170, 1132
BsnI GGCC 6 cut(s) 336, 342, 572, 596, 632, 989
BsoBI CYCGRG 3 cut(s) 165, 455, 1391
Bsp1286I GDGCHC 2 cut(s) 456, 609
Bsp13I TCCGGA 2 cut(s) 370, 580
Bsp143I GATC 8 cut(s) 139, 298, 367, 433, 768, 949, 1302, 1447
Bsp1720I GCTNAGC 1 cut(s) 1034
BspANI GGCC 6 cut(s) 336, 342, 572, 596, 632, 989
BspCNI CTCAG 7 cut(s) 156, 239, 315, 679, 877, 976, 1026
BspEI TCCGGA 2 cut(s) 370, 580
BspFNI CGCG 5 cut(s) 48, 353, 548, 590, 599
BspLI GGNNCC 5 cut(s) 453, 579, 882, 951, 1406
BspPI GGATC 7 cut(s) 306, 362, 776, 944, 957, 1297, 1442
BspQI GCTCTTC 1 cut(s) 309
BsrFI RCCGGY 1 cut(s) 1046
BssAI RCCGGY 1 cut(s) 1046
BssECI CCNNGG 6 cut(s) 267, 337, 456, 551, 573, 1557
BssMI GATC 8 cut(s) 139, 298, 367, 433, 768, 949, 1302, 1447
BssNI GRCGYC 6 cut(s) 80, 273, 348, 384, 402, 474
BssSI CACGAG 1 cut(s) 214
BssT1I CCWWGG 2 cut(s) 267, 573
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 5 cut(s) 311, 569, 766, 789, 1583
Bst4CI ACNGT 6 cut(s) 716, 811, 946, 1459, 1472, 1477
Bst6I CTCTTC 1 cut(s) 309
BstACI GRCGYC 6 cut(s) 80, 273, 348, 384, 402, 474
BstC8I GCNNGC 1 cut(s) 900
BstDEI CTNAG 7 cut(s) 143, 226, 302, 666, 885, 984, 1034
BstDSI CCRYGG 2 cut(s) 337, 551
BstENI CCTNNNNNAGG 1 cut(s) 732
BstF5I GGATG 6 cut(s) 823, 928, 1030, 1375, 1387, 1615
BstFNI CGCG 5 cut(s) 48, 353, 548, 590, 599
BstH2I RGCGCY 2 cut(s) 233, 1004
BstHHI GCGC 6 cut(s) 50, 232, 355, 550, 828, 1003
BstKTI GATC 8 cut(s) 142, 301, 370, 436, 771, 952, 1305, 1450
BstMAI GTCTC 5 cut(s) 152, 161, 500, 669, 1304
BstMBI GATC 8 cut(s) 139, 298, 367, 433, 768, 949, 1302, 1447
BstMCI CGRYCG 2 cut(s) 324, 597
BstMWI GCNNNNNNNGC 5 cut(s) 325, 564, 596, 1534, 1587
BstNI CCWGG 5 cut(s) 311, 569, 766, 789, 1583
BstNSI RCATGY 1 cut(s) 1511
BstSCI CCNGG 6 cut(s) 309, 567, 764, 787, 1305, 1581
BstUI CGCG 5 cut(s) 48, 353, 548, 590, 599
BstV1I GCAGC 5 cut(s) 340, 889, 892, 1235, 1602
BstV2I GAAGAC 1 cut(s) 785
BstX2I RGATCY 4 cut(s) 139, 298, 367, 949
BstYI RGATCY 4 cut(s) 139, 298, 367, 949
BstZI CGGCCG 1 cut(s) 594
BsuRI GGCC 6 cut(s) 336, 342, 572, 596, 632, 989
BtgI CCRYGG 2 cut(s) 337, 551
BtsCI GGATG 6 cut(s) 823, 928, 1030, 1375, 1387, 1615
BtsI GCAGTG 1 cut(s) 562
BtsIMutI CAGTG 4 cut(s) 562, 816, 951, 1482
Cac8I GCNNGC 1 cut(s) 900
CaiI CAGNNNCTG 1 cut(s) 788
CciNI GCGGCCGC 1 cut(s) 594
CfoI GCGC 6 cut(s) 50, 232, 355, 550, 828, 1003
Cfr10I RCCGGY 1 cut(s) 1046
Cfr13I GGNCC 5 cut(s) 334, 744, 785, 881, 1006
CseI GACGC 4 cut(s) 281, 356, 392, 410
CsiI ACCWGGT 1 cut(s) 787
Csp6I GTAC 3 cut(s) 445, 586, 1473
CviAII CATG 1 cut(s) 1508
CviQI GTAC 3 cut(s) 445, 586, 1473
DdeI CTNAG 7 cut(s) 143, 226, 302, 666, 885, 984, 1034
DpnI GATC 8 cut(s) 141, 300, 369, 435, 770, 951, 1304, 1449
DpnII GATC 8 cut(s) 139, 298, 367, 433, 768, 949, 1302, 1447
DrdI GACNNNNNNGTC 1 cut(s) 278
DriI GACNNNNNGTC 1 cut(s) 103
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 3 cut(s) 340, 594, 630
EagI CGGCCG 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 309
Eam1105I GACNNNNNGTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 309
EciI GGCGGA 1 cut(s) 630
Ecl136II GAGCTC 1 cut(s) 607
EclXI CGGCCG 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 267, 573
Eco147I AGGCCT 1 cut(s) 572
Eco24I GRGCYC 2 cut(s) 456, 609
Eco47I GGWCC 4 cut(s) 744, 785, 881, 1006
Eco47III AGCGCT 1 cut(s) 1002
Eco52I CGGCCG 1 cut(s) 594
Eco53kI GAGCTC 1 cut(s) 607
Eco57I CTGAAG 2 cut(s) 484, 691
Eco88I CYCGRG 3 cut(s) 165, 455, 1391
EcoICRI GAGCTC 1 cut(s) 607
EcoNI CCTNNNNNAGG 1 cut(s) 732
EcoO109I RGGNCCY 2 cut(s) 785, 881
EcoRI GAATTC 1 cut(s) 377
EcoRII CCWGG 5 cut(s) 309, 567, 764, 787, 1581
EcoT14I CCWWGG 2 cut(s) 267, 573
EcoT22I ATGCAT 1 cut(s) 1513
EcoT38I GRGCYC 2 cut(s) 456, 609
ErhI CCWWGG 2 cut(s) 267, 573
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 1 cut(s) 1511
FaiI YATR 6 cut(s) 87, 675, 732, 975, 1290, 1509
FatI CATG 1 cut(s) 1507
FauI CCCGC 2 cut(s) 53, 260
FblI GTMKAC 3 cut(s) 204, 282, 462
FokI GGATG 5 cut(s) 830, 935, 1037, 1382, 1394
FriOI GRGCYC 2 cut(s) 456, 609
FspBI CTAG 1 cut(s) 1119
GlaI GCGC 6 cut(s) 49, 231, 354, 549, 827, 1002
GsaI CCCAGC 3 cut(s) 260, 1001, 1430
HaeII RGCGCY 2 cut(s) 233, 1004
HaeIII GGCC 6 cut(s) 336, 342, 572, 596, 632, 989
HapII CCGG 4 cut(s) 371, 581, 1047, 1307
HgaI GACGC 4 cut(s) 281, 356, 392, 410
HhaI GCGC 6 cut(s) 50, 232, 355, 550, 828, 1003
Hin1I GRCGYC 6 cut(s) 80, 273, 348, 384, 402, 474
Hin1II CATG 1 cut(s) 1511
Hin6I GCGC 6 cut(s) 48, 230, 353, 548, 826, 1001
HinP1I GCGC 6 cut(s) 48, 230, 353, 548, 826, 1001
HincII GTYRAC 5 cut(s) 11, 205, 283, 463, 931
HindII GTYRAC 5 cut(s) 11, 205, 283, 463, 931
HindIII AAGCTT 2 cut(s) 1094, 1281
HinfI GANTC 9 cut(s) 90, 98, 201, 206, 861, 980, 1190, 1373, 1562
HpaII CCGG 4 cut(s) 371, 581, 1047, 1307
Hpy166II GTNNAC 7 cut(s) 11, 205, 221, 283, 463, 721, 931
Hpy188I TCNGA 6 cut(s) 418, 841, 985, 1297, 1558, 1567
Hpy8I GTNNAC 7 cut(s) 11, 205, 221, 283, 463, 721, 931
HpyAV CCTTC 6 cut(s) 463, 481, 1143, 1192, 1372, 1600
HpyCH4III ACNGT 6 cut(s) 716, 811, 946, 1459, 1472, 1477
HpyCH4IV ACGT 3 cut(s) 80, 474, 801
HpyCH4V TGCA 5 cut(s) 682, 821, 1067, 1088, 1511
HpyF10VI GCNNNNNNNGC 5 cut(s) 325, 564, 596, 1534, 1587
HpyF3I CTNAG 7 cut(s) 143, 226, 302, 666, 885, 984, 1034
HpySE526I ACGT 3 cut(s) 80, 474, 801
Hsp92I GRCGYC 6 cut(s) 80, 273, 348, 384, 402, 474
Hsp92II CATG 1 cut(s) 1511
HspAI GCGC 6 cut(s) 48, 230, 353, 548, 826, 1001
Kpn2I TCCGGA 2 cut(s) 370, 580
Kzo9I GATC 8 cut(s) 139, 298, 367, 433, 768, 949, 1302, 1447
LguI GCTCTTC 1 cut(s) 309
LmnI GCTCC 5 cut(s) 242, 451, 583, 604, 1533
Lsp1109I GCAGC 5 cut(s) 340, 889, 892, 1235, 1602
LweI GCATC 5 cut(s) 631, 808, 1453, 1520, 1558
MabI ACCWGGT 1 cut(s) 787
MaeI CTAG 1 cut(s) 1119
MaeII ACGT 3 cut(s) 80, 474, 801
MaeIII GTNAC 3 cut(s) 760, 802, 1108
MalI GATC 8 cut(s) 141, 300, 369, 435, 770, 951, 1304, 1449
MboI GATC 8 cut(s) 139, 298, 367, 433, 768, 949, 1302, 1447
MfeI CAATTG 1 cut(s) 1068
MflI RGATCY 4 cut(s) 139, 298, 367, 949
MhlI GDGCHC 2 cut(s) 456, 609
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 6 cut(s) 377, 636, 848, 1052, 1068, 1613
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 7 cut(s) 92, 99, 200, 210, 974, 1184, 1382
MmeI TCCRAC 1 cut(s) 1321
Mox20I TGGCCA 1 cut(s) 632
Mph1103I ATGCAT 1 cut(s) 1513
MroI TCCGGA 2 cut(s) 370, 580
MroXI GAANNNNTTC 1 cut(s) 524
MscI TGGCCA 1 cut(s) 632
MseI TTAA 3 cut(s) 120, 513, 1278
Msp20I TGGCCA 1 cut(s) 632
MspA1I CMGCKG 2 cut(s) 1165, 1430
MspI CCGG 4 cut(s) 371, 581, 1047, 1307
MspR9I CCNGG 6 cut(s) 311, 569, 766, 789, 1307, 1583
MunI CAATTG 1 cut(s) 1068
Mva1269I GAATGC 2 cut(s) 170, 1132
MvaI CCWGG 5 cut(s) 311, 569, 766, 789, 1583
MvnI CGCG 5 cut(s) 48, 353, 548, 590, 599
MwoI GCNNNNNNNGC 5 cut(s) 325, 564, 596, 1534, 1587
NciI CCSGG 1 cut(s) 1307
NdeII GATC 8 cut(s) 139, 298, 367, 433, 768, 949, 1302, 1447
NlaIII CATG 1 cut(s) 1511
NlaIV GGNNCC 5 cut(s) 453, 579, 882, 951, 1406
NmuCI GTSAC 2 cut(s) 802, 1108
NotI GCGGCCGC 1 cut(s) 594
NsiI ATGCAT 1 cut(s) 1513
NspI RCATGY 1 cut(s) 1511
PaeR7I CTCGAG 2 cut(s) 165, 1391
PceI AGGCCT 1 cut(s) 572
PciSI GCTCTTC 1 cut(s) 309
PcsI WCGNNNNNNNCGW 6 cut(s) 279, 288, 402, 414, 429, 471
PctI GAATGC 2 cut(s) 170, 1132
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 2 cut(s) 861, 1562
PflFI GACNNNGTC 1 cut(s) 476
PflMI CCANNNNNTGG 2 cut(s) 574, 946
PfoI TCCNGGA 2 cut(s) 309, 1305
PleI GAGTC 7 cut(s) 92, 98, 200, 209, 974, 1184, 1381
PpsI GAGTC 7 cut(s) 92, 98, 200, 209, 974, 1184, 1381
PpuMI RGGWCCY 2 cut(s) 785, 881
Psp124BI GAGCTC 1 cut(s) 609
Psp5II RGGWCCY 2 cut(s) 785, 881
Psp6I CCWGG 5 cut(s) 309, 567, 764, 787, 1581
PspFI CCCAGC 3 cut(s) 256, 997, 1426
PspGI CCWGG 5 cut(s) 309, 567, 764, 787, 1581
PspN4I GGNNCC 5 cut(s) 453, 579, 882, 951, 1406
PspPI GGNCC 5 cut(s) 334, 744, 785, 881, 1006
PspPPI RGGWCCY 2 cut(s) 785, 881
PspXI VCTCGAGB 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 4 cut(s) 139, 298, 367, 949
PsyI GACNNNGTC 1 cut(s) 476
PvuII CAGCTG 2 cut(s) 1165, 1430
RsaI GTAC 3 cut(s) 446, 587, 1474
RsaNI GTAC 3 cut(s) 445, 586, 1473
SacI GAGCTC 1 cut(s) 609
SalI GTCGAC 3 cut(s) 203, 281, 461
SapI GCTCTTC 1 cut(s) 309
SaqAI TTAA 3 cut(s) 120, 513, 1278
Sau3AI GATC 8 cut(s) 139, 298, 367, 433, 768, 949, 1302, 1447
Sau96I GGNCC 5 cut(s) 334, 744, 785, 881, 1006
SchI GAGTC 7 cut(s) 92, 99, 200, 210, 974, 1184, 1382
ScrFI CCNGG 6 cut(s) 311, 569, 766, 789, 1307, 1583
SduI GDGCHC 2 cut(s) 456, 609
SexAI ACCWGGT 1 cut(s) 787
SfaNI GCATC 5 cut(s) 631, 808, 1453, 1520, 1558
Sfr274I CTCGAG 2 cut(s) 165, 1391
SgrDI CGTCGACG 1 cut(s) 281
SinI GGWCC 4 cut(s) 744, 785, 881, 1006
SlaI CTCGAG 2 cut(s) 165, 1391
SmlI CTYRAG 7 cut(s) 151, 165, 608, 917, 1265, 1391, 1499
SmoI CTYRAG 7 cut(s) 151, 165, 608, 917, 1265, 1391, 1499
Sse9I AATT 6 cut(s) 377, 636, 848, 1052, 1068, 1613
SseBI AGGCCT 1 cut(s) 572
SspMI CTAG 1 cut(s) 1119
SstI GAGCTC 1 cut(s) 609
StuI AGGCCT 1 cut(s) 572
StyD4I CCNGG 6 cut(s) 309, 567, 764, 787, 1305, 1581
StyI CCWWGG 2 cut(s) 267, 573
TaaI ACNGT 6 cut(s) 716, 811, 946, 1459, 1472, 1477
TaiI ACGT 3 cut(s) 83, 477, 804
TaqI TCGA 9 cut(s) 166, 204, 282, 381, 423, 462, 1056, 1376, 1392
TaqII GACCGA 1 cut(s) 338
TasI AATT 6 cut(s) 377, 636, 848, 1052, 1068, 1613
TauI GCSGC 6 cut(s) 278, 353, 358, 593, 596, 599
TfiI GAWTC 2 cut(s) 861, 1562
Tru1I TTAA 3 cut(s) 120, 513, 1278
Tru9I TTAA 3 cut(s) 120, 513, 1278
TscAI CASTG 4 cut(s) 569, 816, 951, 1482
TseFI GTSAC 2 cut(s) 802, 1108
TseI GCWGC 5 cut(s) 328, 902, 905, 1223, 1590
Tsp45I GTSAC 2 cut(s) 802, 1108
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 617, 662
TspRI CASTG 4 cut(s) 569, 816, 951, 1482
Tth111I GACNNNGTC 1 cut(s) 476
Van91I CCANNNNNTGG 2 cut(s) 574, 946
VpaK11BI GGWCC 4 cut(s) 744, 785, 881, 1006
XagI CCTNNNNNAGG 1 cut(s) 732
XapI RAATTY 3 cut(s) 377, 848, 1052
XceI RCATGY 1 cut(s) 1511
XhoI CTCGAG 2 cut(s) 165, 1391
XmiI GTMKAC 3 cut(s) 204, 282, 462
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 1119
ZraI GACGTC 2 cut(s) 81, 475
Zsp2I ATGCAT 1 cut(s) 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.