Rroxscaffold_1G00023720

ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013388
Physical Location & Seq
Reverse (-)
29313519 .. 29316927
3409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00023720.1

Sequence Viewer

Length: 861 bp
ATGTTGCTCAGAAGAAGACTCACTTCCCTTCTCTCCACCTCCATCCTCTGCCCCAAACCCAGTCTCTCTCTCACTCCCACTCCCTCGCCCCAATCCTCCCTACATTTCCTTGCTCAATGCACCAACCCTAACCCTAGCTTCAGCAGCAATGGCAAATCCGCCGCAGCCTTGGGGATTGTTCAGGGAGTGAGGGCGTACAGTAGTCTGCTGAGCTTGAACGATCTGAGACCCAAAGTGCCCAGGAAGCAGAAGACCAGGAAAGGCCGGGGGATTGGGTCCGGGAAAGGCAAGACGGCTGGGCGAGGCCACAAGGGTCAGAAGGCCAGGGGCTCCGGGAAGTTGGGGTTCGAAGGTGGACAGACCCCGCTGCGACGTCGTTTGCCCAAGCGTGGGTTTAAGAACCCCTTTAGTCTCACTTTTCAGCCAGTAGGATTAGGAAAGATTGCCAAACTAATAAATGCGGGAAAGATTGATTCTTCAGAGTTGATTACAATGAAGACTCTCAAGGATACAGGGGCAATAGGGAAGCAGATAAAAGATGGAGTGAGATTAATGGGACGTGGTGCTGAACACATCAAGTGGCCAATTAATTTAGAGGTATCACGTGTCACCGTAAGGGCAAAGGATGCAGTTGAAGCTGCAGGAGGGTCTGTCAGAAGAGTGTATTACAACAAGTTAGGCTTTCGAGCTTTGCTCACACCTGAGTGGTTTGAAAAGAAGGGGAGATTATTGCCAAGAGCAGCCAGACCTCCACCAAAACAGAAGGATAAGGTTGACAGCATTGGCCGCTTGCCAGCCCCAACCAAACCTATTCCATTTTTGACTGAAGAAAACGAGGCGGCCTCCACTTCATCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27A

Protein Analysis

286

Amino Acids

30.92

Weight (kDa)

11.24

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27A PF00828 142 - 218 6.9e-17 Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64670
fragaria_vesca FvH4_3g30590
malus_domestica MD17G1181300.v1.1
prunus_persica Prupe.6G163200_v2.0.a1 Prupe.8G100400_v2.0.a1
pyrus_communis pycom17g19070
rosa_chinensis RchiOBHm_Chr5g0056641
rosa_laevigata RLG00000035114
rosa_multiflora Rmu_sc0004101.1_g000037 Rmu_sc0008982.1_g000001
rosa_roxburghii Rroxscaffold_1G00023720
rosa_rugosa Rorug05G0301100
rosa_samantha Rh5AG371400 Rh5BG383100 Rh5CG406300 Rh5DG395900
rosa_wichuraiana Rw0G018930 Rw5G034810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 376
AciI CCGC 6 cut(s) 159, 162, 365, 461, 787, 839
AcoI YGGCCR 2 cut(s) 581, 784
AcuI CTGAAG 3 cut(s) 124, 462, 846
AcvI CACGTG 1 cut(s) 605
AcyI GRCGYC 1 cut(s) 373
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 389, 390
AflIII ACRYGT 1 cut(s) 604
AgsI TTSAA 3 cut(s) 217, 635, 713
AjiI CACGTC 1 cut(s) 560
AjnI CCWGG 3 cut(s) 239, 254, 323
AleI CACNNNNGTG 1 cut(s) 703
AluBI AGCT 4 cut(s) 138, 213, 638, 689
AluI AGCT 4 cut(s) 138, 213, 638, 689
Alw26I GTCTC 3 cut(s) 68, 220, 416
AoxI GGCC 6 cut(s) 262, 304, 321, 581, 784, 840
ApeKI GCWGC 5 cut(s) 144, 164, 367, 638, 740
AseI ATTAAT 2 cut(s) 551, 588
AspS9I GGNCC 1 cut(s) 276
AsuC2I CCSGG 3 cut(s) 266, 280, 334
AsuHPI GGTGA 1 cut(s) 601
AsuII TTCGAA 1 cut(s) 348
AvaII GGWCC 1 cut(s) 276
BaeGI GKGCMC 1 cut(s) 240
BalI TGGCCA 1 cut(s) 583
BanII GRGCYC 1 cut(s) 332
BbrPI CACGTG 1 cut(s) 605
BbsI GAAGAC 3 cut(s) 22, 257, 503
BbvI GCAGC 5 cut(s) 156, 176, 354, 625, 752
BccI CCATC 2 cut(s) 50, 533
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 3 cut(s) 241, 256, 325
BciVI GTATCC 1 cut(s) 502
BcnI CCSGG 3 cut(s) 266, 280, 334
BcoDI GTCTC 3 cut(s) 68, 220, 416
BfaI CTAG 1 cut(s) 135
BfmI CTRYAG 1 cut(s) 639
BfuI GTATCC 1 cut(s) 502
BisI GCNGC 8 cut(s) 145, 162, 165, 368, 639, 741, 787, 840
BlpI GCTNAGC 1 cut(s) 209
BlsI GCNGC 8 cut(s) 146, 163, 166, 369, 640, 742, 788, 841
Bme1390I CCNGG 6 cut(s) 241, 256, 266, 280, 325, 334
Bme18I GGWCC 1 cut(s) 276
BmgBI CACGTC 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 2 cut(s) 277, 331
BmrFI CCNGG 6 cut(s) 241, 256, 266, 280, 325, 334
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 616
BmuI ACTGGG 1 cut(s) 54
BpiI GAAGAC 3 cut(s) 22, 257, 503
BplI GAGNNNNNCTC 4 cut(s) 678, 710, 827, 859
Bpu1102I GCTNAGC 1 cut(s) 209
Bpu14I TTCGAA 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 3 cut(s) 266, 280, 334
BsaAI YACGTR 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 373
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 4 cut(s) 168, 239, 265, 324
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 636, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 390
Bse1I ACTGG 2 cut(s) 60, 425
Bse3DI GCAATG 1 cut(s) 154
BseBI CCWGG 3 cut(s) 241, 256, 325
BseDI CCNNGG 4 cut(s) 168, 239, 265, 324
BseGI GGATG 2 cut(s) 42, 631
BseLI CCNNNNNNNGG 2 cut(s) 389, 390
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 4 cut(s) 22, 200, 215, 693
BseNI ACTGG 2 cut(s) 60, 425
BseSI GKGCMC 1 cut(s) 240
BseXI GCAGC 5 cut(s) 156, 176, 354, 625, 752
BseYI CCCAGC 1 cut(s) 296
BshFI GGCC 6 cut(s) 264, 306, 323, 583, 786, 842
BsiSI CCGG 3 cut(s) 265, 279, 333
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 2 cut(s) 389, 390
BsmAI GTCTC 3 cut(s) 68, 220, 416
BsmFI GGGAC 1 cut(s) 570
BsnI GGCC 6 cut(s) 264, 306, 323, 583, 786, 842
Bso31I GGTCTC 1 cut(s) 220
Bsp119I TTCGAA 1 cut(s) 348
Bsp1286I GDGCHC 2 cut(s) 240, 332
Bsp143I GATC 1 cut(s) 220
Bsp1720I GCTNAGC 1 cut(s) 209
BspACI CCGC 6 cut(s) 159, 162, 365, 461, 787, 839
BspANI GGCC 6 cut(s) 264, 306, 323, 583, 786, 842
BspCNI CTCAG 4 cut(s) 21, 201, 216, 694
BspLI GGNNCC 2 cut(s) 277, 331
BspMAI CTGCAG 1 cut(s) 643
BspT104I TTCGAA 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 154
BsrI ACTGG 2 cut(s) 60, 425
BssECI CCNNGG 4 cut(s) 168, 239, 265, 324
BssMI GATC 1 cut(s) 220
BssNI GRCGYC 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 3 cut(s) 241, 256, 325
Bst4CI ACNGT 2 cut(s) 200, 613
Bst6I CTCTTC 1 cut(s) 652
BstACI GRCGYC 1 cut(s) 373
BstAPI GCANNNNNTGC 1 cut(s) 626
BstBAI YACGTR 1 cut(s) 605
BstBI TTCGAA 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 791, 795
BstDEI CTNAG 4 cut(s) 8, 209, 224, 702
BstF5I GGATG 2 cut(s) 42, 631
BstKTI GATC 1 cut(s) 223
BstMAI GTCTC 3 cut(s) 68, 220, 416
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 6 cut(s) 144, 150, 244, 626, 635, 786
BstNI CCWGG 3 cut(s) 241, 256, 325
BstSCI CCNGG 6 cut(s) 239, 254, 264, 278, 323, 332
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 240
BstV1I GCAGC 5 cut(s) 156, 176, 354, 625, 752
BstV2I GAAGAC 3 cut(s) 22, 257, 503
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 6 cut(s) 264, 306, 323, 583, 786, 842
BtrI CACGTC 1 cut(s) 560
BtsCI GGATG 2 cut(s) 42, 631
Cac8I GCNNGC 2 cut(s) 791, 795
Cfr13I GGNCC 1 cut(s) 276
Csp6I GTAC 1 cut(s) 196
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 4 cut(s) 8, 209, 224, 702
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
EaeI YGGCCR 2 cut(s) 581, 784
Eam1104I CTCTTC 1 cut(s) 652
EarI CTCTTC 1 cut(s) 652
EciI GGCGGA 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 332
Eco31I GGTCTC 1 cut(s) 220
Eco47I GGWCC 1 cut(s) 276
Eco57I CTGAAG 3 cut(s) 124, 462, 846
Eco72I CACGTG 1 cut(s) 605
EcoRII CCWGG 3 cut(s) 239, 254, 323
EcoT14I CCWWGG 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 332
ErhI CCWWGG 1 cut(s) 168
FalI AAGNNNNNCTT 6 cut(s) 7, 39, 389, 421, 665, 697
FaqI GGGAC 1 cut(s) 570
FauI CCCGC 2 cut(s) 372, 454
Fnu4HI GCNGC 8 cut(s) 145, 162, 165, 368, 639, 741, 787, 840
FokI GGATG 2 cut(s) 29, 638
FriOI GRGCYC 1 cut(s) 332
Fsp4HI GCNGC 8 cut(s) 145, 162, 165, 368, 639, 741, 787, 840
FspBI CTAG 1 cut(s) 135
GluI GCNGC 8 cut(s) 145, 162, 165, 368, 639, 741, 787, 840
GsaI CCCAGC 1 cut(s) 300
HaeIII GGCC 6 cut(s) 264, 306, 323, 583, 786, 842
HapII CCGG 3 cut(s) 265, 279, 333
Hin1I GRCGYC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 3 cut(s) 18, 473, 499
HpaII CCGG 3 cut(s) 265, 279, 333
HphI GGTGA 1 cut(s) 601
Hpy166II GTNNAC 2 cut(s) 356, 775
Hpy188I TCNGA 5 cut(s) 11, 225, 318, 481, 656
Hpy8I GTNNAC 2 cut(s) 356, 775
Hpy99I CGWCG 2 cut(s) 375, 378
HpyAV CCTTC 5 cut(s) 38, 313, 344, 712, 757
HpyCH4III ACNGT 2 cut(s) 200, 613
HpyCH4IV ACGT 3 cut(s) 373, 559, 604
HpyCH4V TGCA 3 cut(s) 120, 629, 641
HpyF10VI GCNNNNNNNGC 6 cut(s) 144, 150, 244, 626, 635, 786
HpyF3I CTNAG 4 cut(s) 8, 209, 224, 702
HpySE526I ACGT 3 cut(s) 373, 559, 604
Hsp92I GRCGYC 1 cut(s) 373
Kzo9I GATC 1 cut(s) 220
LmnI GCTCC 1 cut(s) 335
Lsp1109I GCAGC 5 cut(s) 156, 176, 354, 625, 752
LweI GCATC 1 cut(s) 616
MaeI CTAG 1 cut(s) 135
MaeII ACGT 3 cut(s) 373, 559, 604
MaeIII GTNAC 1 cut(s) 607
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MboII GAAGA 7 cut(s) 24, 27, 262, 468, 508, 669, 839
MhlI GDGCHC 2 cut(s) 240, 332
MlsI TGGCCA 1 cut(s) 583
MluCI AATT 2 cut(s) 585, 589
MluNI TGGCCA 1 cut(s) 583
MlyI GAGTC 2 cut(s) 12, 493
Mox20I TGGCCA 1 cut(s) 583
MscI TGGCCA 1 cut(s) 583
MseI TTAA 3 cut(s) 396, 551, 588
MslI CAYNNNNRTG 1 cut(s) 703
Msp20I TGGCCA 1 cut(s) 583
MspA1I CMGCKG 1 cut(s) 367
MspI CCGG 3 cut(s) 265, 279, 333
MspR9I CCNGG 6 cut(s) 241, 256, 266, 280, 325, 334
MvaI CCWGG 3 cut(s) 241, 256, 325
MwoI GCNNNNNNNGC 6 cut(s) 144, 150, 244, 626, 635, 786
NciI CCSGG 3 cut(s) 266, 280, 334
NdeII GATC 1 cut(s) 220
NlaIV GGNNCC 2 cut(s) 277, 331
NmuCI GTSAC 1 cut(s) 607
NspV TTCGAA 1 cut(s) 348
OliI CACNNNNGTG 1 cut(s) 703
PfeI GAWTC 1 cut(s) 473
PfoI TCCNGGA 2 cut(s) 278, 332
PkrI GCNGC 8 cut(s) 146, 163, 166, 369, 640, 742, 788, 841
PleI GAGTC 2 cut(s) 12, 493
PmaCI CACGTG 1 cut(s) 605
PmlI CACGTG 1 cut(s) 605
PpsI GAGTC 2 cut(s) 12, 493
Ppu21I YACGTR 1 cut(s) 605
PshBI ATTAAT 2 cut(s) 551, 588
Psp6I CCWGG 3 cut(s) 239, 254, 323
PspCI CACGTG 1 cut(s) 605
PspFI CCCAGC 1 cut(s) 296
PspGI CCWGG 3 cut(s) 239, 254, 323
PspN4I GGNNCC 2 cut(s) 277, 331
PspPI GGNCC 1 cut(s) 276
PstI CTGCAG 1 cut(s) 643
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 1 cut(s) 703
SaqAI TTAA 3 cut(s) 396, 551, 588
SatI GCNGC 8 cut(s) 145, 162, 165, 368, 639, 741, 787, 840
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 1 cut(s) 276
SchI GAGTC 2 cut(s) 12, 493
ScrFI CCNGG 6 cut(s) 241, 256, 266, 280, 325, 334
SduI GDGCHC 2 cut(s) 240, 332
SfaNI GCATC 1 cut(s) 616
SfcI CTRYAG 1 cut(s) 639
SfuI TTCGAA 1 cut(s) 348
SinI GGWCC 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 703
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 2 cut(s) 585, 589
SsiI CCGC 6 cut(s) 159, 162, 365, 461, 787, 839
SspMI CTAG 1 cut(s) 135
StyD4I CCNGG 6 cut(s) 239, 254, 264, 278, 323, 332
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 200, 613
TaiI ACGT 3 cut(s) 376, 562, 607
TaqI TCGA 2 cut(s) 348, 685
TasI AATT 2 cut(s) 585, 589
TauI GCSGC 3 cut(s) 164, 789, 842
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 3 cut(s) 396, 551, 588
Tru9I TTAA 3 cut(s) 396, 551, 588
TseFI GTSAC 1 cut(s) 607
TseI GCWGC 5 cut(s) 144, 164, 367, 638, 740
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 2 cut(s) 509, 840
VpaK11BI GGWCC 1 cut(s) 276
VspI ATTAAT 2 cut(s) 551, 588
XspI CTAG 1 cut(s) 135
ZraI GACGTC 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.