Rroxscaffold_1G00049090

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017060
Physical Location & Seq
Forward (+)
69259728 .. 69262255
2528 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00049090.1

Sequence Viewer

Length: 606 bp
ATGCTCCACGCCTCTTCTTCTATCACCACTAACTCAGAGGTGCCTGCTTTGCTTGGCCGTATCCCATCTACTGTTCGATACCAAGCTACTGCAGCTACATATCTTGGACGACTTAGAACAACCTACAAGCGTTCCATCTCTTCTGCCCAAGCTATTCATATGCCTTCTGTCCAACAGACCCTTGCTACTAGAACTAAGGTTGTTGACTTTCTTGCACCATACCAAAGAGGAGGAAATATTGGGTTGTTTGGTGGGGTTGGTGTAATGAAAAACTATGCTTACTATTCAGCTCAACCTGGTGGCAAAAGTGGAGGATTCAAAAAACAGCGCAAGGGAAGAATGAAAGGACTATCTTGCCGAGGTAATCGTATCAGCTTTGGTAAATATGGTCTTCAAGCTCTTGAACCTGCTTGGATCACAGCTAGACAAATAGAAGCAGGGCGGAGAGCAATGTCACGAAATGCACGTGGCGGAGGGAAAATATGGGTACGAGTAATTTCAGACAAACCAGTTACAGCAAAGCCTACAGAAGTACGTATGGGCAAGGGGAAAGGAGCTGTCTCGCATATGGTGGCTGTTGTTTTACCCAGGCAGAATACTTTATGA

Protein Analysis

201

Amino Acids

21.76

Weight (kDa)

11.4

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L16 PF00252 106 - 197 2.5e-30 Ribosomal protein L16p/L10e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017060)

Species Orthologous Gene IDs
malus_domestica MD01G1006600.v1.1
rosa_chinensis RchiOBHm_Chr5g0031171
rosa_laevigata RLG00000033288
rosa_multiflora Rmu_sc0011257.1_g000004 Rmu_sc0027814.1_g000001 Rmu_sc0038845.1_g000001
rosa_roxburghii Rroxscaffold_1G00049090
rosa_samantha Rh5AG216400 Rh5BG215700 Rh5CG239200 Rh5CG239300 Rh5DG220600
rosa_wichuraiana Rw5G019780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 415
AccB1I GGYRCC 1 cut(s) 40
AciI CCGC 2 cut(s) 442, 471
AclWI GGATC 1 cut(s) 422
AcoI YGGCCR 1 cut(s) 55
AcvI CACGTG 1 cut(s) 467
AfaI GTAC 2 cut(s) 489, 534
AgsI TTSAA 3 cut(s) 319, 395, 404
AjnI CCWGG 2 cut(s) 295, 587
AluBI AGCT 8 cut(s) 86, 95, 152, 290, 375, 398, 422, 557
AluI AGCT 8 cut(s) 86, 95, 152, 290, 375, 398, 422, 557
Alw26I GTCTC 1 cut(s) 565
AlwI GGATC 1 cut(s) 422
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 92
AspLEI GCGC 1 cut(s) 330
AsuHPI GGTGA 1 cut(s) 16
BanI GGYRCC 1 cut(s) 40
BbrPI CACGTG 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 383
BbvI GCAGC 1 cut(s) 104
BccI CCATC 2 cut(s) 73, 143
BceAI ACGGC 1 cut(s) 42
BciT130I CCWGG 2 cut(s) 297, 589
BciVI GTATCC 1 cut(s) 71
BcoDI GTCTC 1 cut(s) 565
BfaI CTAG 2 cut(s) 189, 423
BfmI CTRYAG 2 cut(s) 90, 525
BfuAI ACCTGC 1 cut(s) 415
BfuI GTATCC 1 cut(s) 71
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme1390I CCNGG 2 cut(s) 297, 589
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 2 cut(s) 297, 589
BpiI GAAGAC 1 cut(s) 383
BsaAI YACGTR 2 cut(s) 467, 536
BsaJI CCNNGG 2 cut(s) 358, 587
Bse1I ACTGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 456
BseBI CCWGG 2 cut(s) 297, 589
BseDI CCNNGG 2 cut(s) 358, 587
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 509
BseRI GAGGAG 1 cut(s) 243
BseXI GCAGC 1 cut(s) 104
BshFI GGCC 1 cut(s) 57
BshNI GGYRCC 1 cut(s) 40
BsmAI GTCTC 1 cut(s) 565
BsnI GGCC 1 cut(s) 57
Bsp143I GATC 1 cut(s) 414
BspACI CCGC 2 cut(s) 442, 471
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 47
BspLI GGNNCC 1 cut(s) 42
BspMAI CTGCAG 1 cut(s) 94
BspMI ACCTGC 1 cut(s) 415
BspPI GGATC 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 456
BsrI ACTGG 1 cut(s) 509
BssECI CCNNGG 2 cut(s) 358, 587
BssMI GATC 1 cut(s) 414
Bst2UI CCWGG 2 cut(s) 297, 589
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 2 cut(s) 19, 145
BstBAI YACGTR 2 cut(s) 467, 536
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 3 cut(s) 34, 113, 195
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 1 cut(s) 417
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 1 cut(s) 414
BstMWI GCNNNNNNNGC 2 cut(s) 49, 92
BstNI CCWGG 2 cut(s) 297, 589
BstSCI CCNGG 2 cut(s) 295, 587
BstSFI CTRYAG 2 cut(s) 90, 525
BstSNI TACGTA 1 cut(s) 536
BstV1I GCAGC 1 cut(s) 104
BstV2I GAAGAC 1 cut(s) 383
BsuI GTATCC 1 cut(s) 71
BsuRI GGCC 1 cut(s) 57
BveI ACCTGC 1 cut(s) 415
Cac8I GCNNGC 1 cut(s) 45
CfoI GCGC 1 cut(s) 330
CsiI ACCWGGT 1 cut(s) 295
Csp6I GTAC 2 cut(s) 488, 533
CviQI GTAC 2 cut(s) 488, 533
DdeI CTNAG 3 cut(s) 34, 113, 195
DpnI GATC 1 cut(s) 416
DpnII GATC 1 cut(s) 414
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 19, 145
EarI CTCTTC 2 cut(s) 19, 145
EciI GGCGGA 2 cut(s) 457, 486
Eco105I TACGTA 1 cut(s) 536
Eco72I CACGTG 1 cut(s) 467
EcoRII CCWGG 2 cut(s) 295, 587
FauNDI CATATG 2 cut(s) 159, 567
Fnu4HI GCNGC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 2 cut(s) 189, 423
GlaI GCGC 1 cut(s) 329
GluI GCNGC 1 cut(s) 93
HaeIII GGCC 1 cut(s) 57
HhaI GCGC 1 cut(s) 330
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 1 cut(s) 315
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 2 cut(s) 37, 502
Hpy188III TCNNGA 2 cut(s) 401, 456
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 174
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 2 cut(s) 466, 535
HpyCH4V TGCA 3 cut(s) 92, 215, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 92
HpyF3I CTNAG 3 cut(s) 34, 113, 195
HpySE526I ACGT 2 cut(s) 466, 535
HspAI GCGC 1 cut(s) 328
Kzo9I GATC 1 cut(s) 414
LmnI GCTCC 2 cut(s) 9, 554
LpnPI CCDG 8 cut(s) 57, 282, 309, 420, 423, 522, 574, 601
Lsp1109I GCAGC 1 cut(s) 104
MabI ACCWGGT 1 cut(s) 295
MaeI CTAG 2 cut(s) 189, 423
MaeII ACGT 2 cut(s) 466, 535
MaeIII GTNAC 2 cut(s) 453, 511
MalI GATC 1 cut(s) 416
MboI GATC 1 cut(s) 414
MboII GAAGA 5 cut(s) 6, 9, 132, 348, 383
MluCI AATT 1 cut(s) 495
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 7 cut(s) 22, 31, 221, 224, 305, 353, 467
MspR9I CCNGG 2 cut(s) 297, 589
MvaI CCWGG 2 cut(s) 297, 589
MwoI GCNNNNNNNGC 2 cut(s) 49, 92
NdeI CATATG 2 cut(s) 159, 567
NdeII GATC 1 cut(s) 414
NlaIV GGNNCC 1 cut(s) 42
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 1 cut(s) 453
PcsI WCGNNNNNNNCGW 1 cut(s) 463
PfeI GAWTC 1 cut(s) 315
PkrI GCNGC 1 cut(s) 94
PmaCI CACGTG 1 cut(s) 467
PmlI CACGTG 1 cut(s) 467
Ppu21I YACGTR 2 cut(s) 467, 536
Psp6I CCWGG 2 cut(s) 295, 587
PspCI CACGTG 1 cut(s) 467
PspGI CCWGG 2 cut(s) 295, 587
PspN4I GGNNCC 1 cut(s) 42
PstI CTGCAG 1 cut(s) 94
RsaI GTAC 2 cut(s) 489, 534
RsaNI GTAC 2 cut(s) 488, 533
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 1 cut(s) 414
ScrFI CCNGG 2 cut(s) 297, 589
SexAI ACCWGGT 1 cut(s) 295
SfcI CTRYAG 2 cut(s) 90, 525
SnaBI TACGTA 1 cut(s) 536
Sse9I AATT 1 cut(s) 495
SsiI CCGC 2 cut(s) 442, 471
SspI AATATT 1 cut(s) 238
SspMI CTAG 2 cut(s) 189, 423
StyD4I CCNGG 2 cut(s) 295, 587
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 2 cut(s) 469, 538
TaqI TCGA 1 cut(s) 76
TasI AATT 1 cut(s) 495
TfiI GAWTC 1 cut(s) 315
TseFI GTSAC 1 cut(s) 453
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 453
TspDTI ATGAA 3 cut(s) 146, 281, 356
XspI CTAG 2 cut(s) 189, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.