Rh5CG239200

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017060
Physical Location & Seq
Reverse (-)
24833365 .. 24833724
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG239200.1

Sequence Viewer

Length: 360 bp
ATGAAAGGACTATCTTGCCGAGGTAATCGTATCAGCTTTGGTAAATATGGTCTTCAAGCGCTTGAACCTGCTTGGATCACAGCTAGACAAATAGAAGCAGGGCGGAGAGCAATGTCACGAAATGCACGTGGCGGAGGGAAAATATGGGTACGAGTAATTTCAGACAAACCAGTTACAGCAAAGCCTACAGAAGTACGTATGGGCAAGGGGAAAGGAGCTGTCTCGCATATGGTGGCTGTTGTTTTACCGGGCAGAATACTTTATGAAGTGAGCGGAGTAGCAGAAAATGTAGCCAGAAGGGCTATTACTATAGCTGGATCCAAAATGCCTATAAAAACTAAATTTGTTGTCTCAGGGTAG

Protein Analysis

119

Amino Acids

12.76

Weight (kDa)

11.41

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L16 PF00252 2 - 116 4.8e-42 Ribosomal protein L16p/L10e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017060)

Species Orthologous Gene IDs
malus_domestica MD01G1006600.v1.1
rosa_chinensis RchiOBHm_Chr5g0031171
rosa_laevigata RLG00000033288
rosa_multiflora Rmu_sc0011257.1_g000004 Rmu_sc0027814.1_g000001 Rmu_sc0038845.1_g000001
rosa_roxburghii Rroxscaffold_1G00049090
rosa_samantha Rh5AG216400 Rh5BG215700 Rh5CG239200 Rh5CG239300 Rh5DG220600
rosa_wichuraiana Rw5G019780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 76
AccBSI CCGCTC 1 cut(s) 273
AciI CCGC 3 cut(s) 103, 132, 273
AclWI GGATC 3 cut(s) 83, 312, 325
AcsI RAATTY 1 cut(s) 341
AcvI CACGTG 1 cut(s) 128
AfaI GTAC 2 cut(s) 150, 195
AfeI AGCGCT 1 cut(s) 60
AgsI TTSAA 2 cut(s) 56, 65
AluBI AGCT 4 cut(s) 36, 83, 218, 314
AluI AGCT 4 cut(s) 36, 83, 218, 314
Alw26I GTCTC 2 cut(s) 226, 355
AlwI GGATC 3 cut(s) 83, 312, 325
Aor51HI AGCGCT 1 cut(s) 60
ApoI RAATTY 1 cut(s) 341
AspLEI GCGC 1 cut(s) 61
AsuC2I CCSGG 1 cut(s) 249
BamHI GGATCC 1 cut(s) 317
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
BbrPI CACGTG 1 cut(s) 128
BbsI GAAGAC 1 cut(s) 44
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 2 cut(s) 226, 355
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 2 cut(s) 186, 309
BfoI RGCGCY 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 76
BglI GCCNNNNNGGC 1 cut(s) 299
Bme1390I CCNGG 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 44
BpuMI CCSGG 1 cut(s) 249
BsaAI YACGTR 2 cut(s) 128, 197
BsaJI CCNNGG 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 19
BseMI GCAATG 1 cut(s) 117
BseNI ACTGG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 248
BsmAI GTCTC 2 cut(s) 226, 355
Bsp143I GATC 2 cut(s) 75, 317
BspACI CCGC 3 cut(s) 103, 132, 273
BspLI GGNNCC 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 76
BspPI GGATC 3 cut(s) 83, 312, 325
BsrBI CCGCTC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 117
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 2 cut(s) 75, 317
BstBAI YACGTR 2 cut(s) 128, 197
BstDEI CTNAG 1 cut(s) 352
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 2 cut(s) 78, 320
BstMAI GTCTC 2 cut(s) 226, 355
BstMBI GATC 2 cut(s) 75, 317
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 2 cut(s) 186, 309
BstSNI TACGTA 1 cut(s) 197
BstV2I GAAGAC 1 cut(s) 44
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BveI ACCTGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 61
Csp6I GTAC 2 cut(s) 149, 194
CviJI RGCY 8 cut(s) 36, 83, 184, 218, 236, 293, 302, 314
CviKI_1 RGCY 8 cut(s) 36, 83, 184, 218, 236, 293, 302, 314
CviQI GTAC 2 cut(s) 149, 194
DdeI CTNAG 1 cut(s) 352
DpnI GATC 2 cut(s) 77, 319
DpnII GATC 2 cut(s) 75, 317
EciI GGCGGA 2 cut(s) 118, 147
Eco105I TACGTA 1 cut(s) 197
Eco47III AGCGCT 1 cut(s) 60
Eco72I CACGTG 1 cut(s) 128
FaiI YATR 8 cut(s) 48, 145, 200, 228, 230, 264, 311, 332
FauNDI CATATG 1 cut(s) 228
FspBI CTAG 1 cut(s) 84
GlaI GCGC 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 62
HapII CCGG 1 cut(s) 248
HhaI GCGC 1 cut(s) 61
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HpaII CCGG 1 cut(s) 248
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 1 cut(s) 117
HpyAV CCTTC 1 cut(s) 291
HpyCH4IV ACGT 2 cut(s) 127, 196
HpyCH4V TGCA 1 cut(s) 125
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 1 cut(s) 352
HpySE526I ACGT 2 cut(s) 127, 196
HspAI GCGC 1 cut(s) 59
Kzo9I GATC 2 cut(s) 75, 317
LmnI GCTCC 1 cut(s) 215
LpnPI CCDG 7 cut(s) 81, 84, 183, 261, 300, 307, 339
MaeI CTAG 1 cut(s) 84
MaeII ACGT 2 cut(s) 127, 196
MaeIII GTNAC 2 cut(s) 114, 172
MalI GATC 2 cut(s) 77, 319
MbiI CCGCTC 1 cut(s) 273
MboI GATC 2 cut(s) 75, 317
MboII GAAGA 1 cut(s) 44
MflI RGATCY 1 cut(s) 317
MluCI AATT 2 cut(s) 156, 341
MnlI CCTC 2 cut(s) 14, 128
MspI CCGG 1 cut(s) 248
MspR9I CCNGG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 299
NciI CCSGG 1 cut(s) 249
NdeI CATATG 1 cut(s) 228
NdeII GATC 2 cut(s) 75, 317
NlaIV GGNNCC 1 cut(s) 319
NmeAIII GCCGAG 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 124
PmaCI CACGTG 1 cut(s) 128
PmlI CACGTG 1 cut(s) 128
Ppu21I YACGTR 2 cut(s) 128, 197
PspCI CACGTG 1 cut(s) 128
PspN4I GGNNCC 1 cut(s) 319
PsuI RGATCY 1 cut(s) 317
RsaI GTAC 2 cut(s) 150, 195
RsaNI GTAC 2 cut(s) 149, 194
Sau3AI GATC 2 cut(s) 75, 317
ScrFI CCNGG 1 cut(s) 249
SetI ASST 8 cut(s) 25, 38, 70, 85, 130, 199, 220, 316
SfcI CTRYAG 2 cut(s) 186, 309
SnaBI TACGTA 1 cut(s) 197
Sse9I AATT 2 cut(s) 156, 341
SsiI CCGC 3 cut(s) 103, 132, 273
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 247
TaiI ACGT 2 cut(s) 130, 199
TasI AATT 2 cut(s) 156, 341
TseFI GTSAC 1 cut(s) 114
Tsp45I GTSAC 1 cut(s) 114
TspDTI ATGAA 2 cut(s) 17, 279
XapI RAATTY 1 cut(s) 341
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.