Rh5CG239300

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017060
Physical Location & Seq
Reverse (-)
24833843 .. 24835493
1651 bp
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UTR
Exon/CDS
Intron
Rh5CG239300.1

Sequence Viewer

Length: 489 bp
ATGGCTTCGCGTCGGCTCTTATCCCCTCTTCTCCGATCCCCTTCCAGATTTTCGATCTCAAACCATAACCCTACCCTATCAACCCCCTCGCAATCGCCTCCCCGCGCTTCGCCGTACGCCCACCTCCTTCGGAATGCTCTTCTTACCGGCCGCCGCATGCTCCACACCTCTTCTTCTTTCACCACTAACTCAGAGGTGCCTGCTTTGCTTGGCCGTATCCCATCTACTGTTCGATACCAAGCTACTGCAGCTACTTATCTTGGAGGACTTGGAACAACCTACAAGCGTTCCATCTCTTCTGCCCAAGCTATTCATGTGCCTTCTGTCCAACAGACCCTTGCTACTAGAACTAAGGCTGTTGACTTTCTTGCACCATACCAAAGAGGAGGAAATATTGGGTTGTTTGGTGGGGTTGGTGTAGGGAAAAACTATGCTTACTATTCAGCTCAACCTGGTGGCAAAAGTGGAGGGTCAGTTTTCAAAAGATAG

Protein Analysis

162

Amino Acids

17.28

Weight (kDa)

11.51

Isoelectric Point (pI)

68.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Synthase_beta PF11421 1 - 43 3e-08 ATP synthase F1 beta subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017060)

Species Orthologous Gene IDs
malus_domestica MD01G1006600.v1.1
rosa_chinensis RchiOBHm_Chr5g0031171
rosa_laevigata RLG00000033288
rosa_multiflora Rmu_sc0011257.1_g000004 Rmu_sc0027814.1_g000001 Rmu_sc0038845.1_g000001
rosa_roxburghii Rroxscaffold_1G00049090
rosa_samantha Rh5AG216400 Rh5BG215700 Rh5CG239200 Rh5CG239300 Rh5DG220600
rosa_wichuraiana Rw5G019780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AccII CGCG 2 cut(s) 10, 105
AciI CCGC 3 cut(s) 103, 151, 154
AclWI GGATC 1 cut(s) 30
AcoI YGGCCR 2 cut(s) 148, 211
AfaI GTAC 1 cut(s) 116
AgsI TTSAA 1 cut(s) 481
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 4 cut(s) 242, 251, 308, 446
AluI AGCT 4 cut(s) 242, 251, 308, 446
AlwI GGATC 1 cut(s) 30
AoxI GGCC 2 cut(s) 148, 211
ApeKI GCWGC 1 cut(s) 248
AspLEI GCGC 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 172
BanI GGYRCC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 260
BccI CCATC 2 cut(s) 229, 299
BceAI ACGGC 2 cut(s) 97, 198
BciT130I CCWGG 1 cut(s) 453
BciVI GTATCC 1 cut(s) 227
BfaI CTAG 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 246
BfuI GTATCC 1 cut(s) 227
BisI GCNGC 3 cut(s) 151, 154, 249
BlsI GCNGC 3 cut(s) 152, 155, 250
Bme1390I CCNGG 1 cut(s) 453
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 453
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bse118I RCCGGY 1 cut(s) 146
BseBI CCWGG 1 cut(s) 453
BseMII CTCAG 1 cut(s) 204
BseRI GAGGAG 1 cut(s) 399
BseX3I CGGCCG 1 cut(s) 148
BseXI GCAGC 1 cut(s) 260
Bsh1236I CGCG 2 cut(s) 10, 105
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 2 cut(s) 150, 213
BshNI GGYRCC 1 cut(s) 196
BsiEI CGRYCG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 147
BsiWI CGTACG 1 cut(s) 114
BsmI GAATGC 1 cut(s) 139
BsnI GGCC 2 cut(s) 150, 213
Bsp143I GATC 2 cut(s) 35, 54
BspACI CCGC 3 cut(s) 103, 151, 154
BspANI GGCC 2 cut(s) 150, 213
BspCNI CTCAG 1 cut(s) 203
BspFNI CGCG 2 cut(s) 10, 105
BspLI GGNNCC 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 250
BspPI GGATC 1 cut(s) 30
BspQI GCTCTTC 1 cut(s) 144
BspT107I GGYRCC 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 146
BssMI GATC 2 cut(s) 35, 54
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 4 cut(s) 33, 144, 175, 301
BstC8I GCNNGC 2 cut(s) 158, 201
BstDEI CTNAG 2 cut(s) 190, 351
BstFNI CGCG 2 cut(s) 10, 105
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 2 cut(s) 38, 57
BstMBI GATC 2 cut(s) 35, 54
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 2 cut(s) 205, 248
BstNI CCWGG 1 cut(s) 453
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 246
BstUI CGCG 2 cut(s) 10, 105
BstV1I GCAGC 1 cut(s) 260
BstZI CGGCCG 1 cut(s) 148
BsuI GTATCC 1 cut(s) 227
BsuRI GGCC 2 cut(s) 150, 213
Cac8I GCNNGC 2 cut(s) 158, 201
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 146
CsiI ACCWGGT 1 cut(s) 451
Csp6I GTAC 1 cut(s) 115
CviAII CATG 2 cut(s) 157, 314
CviJI RGCY 9 cut(s) 5, 16, 150, 213, 242, 251, 308, 356, 446
CviKI_1 RGCY 9 cut(s) 5, 16, 150, 213, 242, 251, 308, 356, 446
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 2 cut(s) 190, 351
DpnI GATC 2 cut(s) 37, 56
DpnII GATC 2 cut(s) 35, 54
EaeI YGGCCR 2 cut(s) 148, 211
EagI CGGCCG 1 cut(s) 148
Eam1104I CTCTTC 4 cut(s) 33, 144, 175, 301
EarI CTCTTC 4 cut(s) 33, 144, 175, 301
EclXI CGGCCG 1 cut(s) 148
Eco52I CGGCCG 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 451
FaeI CATG 2 cut(s) 160, 317
FaiI YATR 5 cut(s) 66, 158, 315, 376, 432
FatI CATG 2 cut(s) 156, 313
FauI CCCGC 1 cut(s) 110
Fnu4HI GCNGC 3 cut(s) 151, 154, 249
Fsp4HI GCNGC 3 cut(s) 151, 154, 249
FspBI CTAG 1 cut(s) 345
GlaI GCGC 1 cut(s) 106
GluI GCNGC 3 cut(s) 151, 154, 249
HaeIII GGCC 2 cut(s) 150, 213
HapII CCGG 1 cut(s) 147
HhaI GCGC 1 cut(s) 107
Hin1II CATG 2 cut(s) 160, 317
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HpaII CCGG 1 cut(s) 147
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 3 cut(s) 35, 132, 193
Hpy188III TCNNGA 1 cut(s) 45
Hpy8I GTNNAC 1 cut(s) 361
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 3 cut(s) 51, 137, 330
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 2 cut(s) 248, 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 248
HpyF3I CTNAG 2 cut(s) 190, 351
Hsp92II CATG 2 cut(s) 160, 317
HspAI GCGC 1 cut(s) 105
Kzo9I GATC 2 cut(s) 35, 54
LguI GCTCTTC 1 cut(s) 144
LmnI GCTCC 1 cut(s) 165
LpnPI CCDG 5 cut(s) 58, 160, 213, 438, 465
Lsp1109I GCAGC 1 cut(s) 260
MabI ACCWGGT 1 cut(s) 451
MaeI CTAG 1 cut(s) 345
MalI GATC 2 cut(s) 37, 56
MboI GATC 2 cut(s) 35, 54
MboII GAAGA 5 cut(s) 20, 131, 162, 165, 288
MmeI TCCRAC 1 cut(s) 352
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 139
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 2 cut(s) 10, 105
MwoI GCNNNNNNNGC 2 cut(s) 205, 248
NdeII GATC 2 cut(s) 35, 54
NlaIII CATG 2 cut(s) 160, 317
NlaIV GGNNCC 1 cut(s) 198
NspI RCATGY 1 cut(s) 160
PaeI GCATGC 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 144
PctI GAATGC 1 cut(s) 139
Pfl23II CGTACG 1 cut(s) 114
PkrI GCNGC 3 cut(s) 152, 155, 250
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspLI CGTACG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 198
PstI CTGCAG 1 cut(s) 250
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 144
SatI GCNGC 3 cut(s) 151, 154, 249
Sau3AI GATC 2 cut(s) 35, 54
ScrFI CCNGG 1 cut(s) 453
SetI ASST 9 cut(s) 126, 170, 198, 244, 253, 281, 310, 448, 454
SexAI ACCWGGT 1 cut(s) 451
SfcI CTRYAG 1 cut(s) 246
SphI GCATGC 1 cut(s) 160
SsiI CCGC 3 cut(s) 103, 151, 154
SspI AATATT 1 cut(s) 394
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 451
TaaI ACNGT 1 cut(s) 229
TaqI TCGA 2 cut(s) 53, 232
TauI GCSGC 2 cut(s) 153, 156
TseI GCWGC 1 cut(s) 248
TspDTI ATGAA 1 cut(s) 302
XceI RCATGY 1 cut(s) 160
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.