Rroxscaffold_1G00064300

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016782
Physical Location & Seq
Reverse (-)
86102872 .. 86105379
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064300.1

Sequence Viewer

Length: 1377 bp
ATGGAGATGGAAAAGAAGGTTGAGTTTGTTGAAGGAAAGGTGGATTGGAAGGGAAGGACAGCTGTGAAACATAAACATGGAGGAATGAGAACTGCTTTTCTCATACTTGCTACATTTGGTTTTGAGAACATGGCAACTTTGGCTTTGGCGGTGAACTTGGTTACATACTTCAATGGGGTGATGCATTTTGAAATATCTGATGCCGCTAACCAACTTACCAACTTCGCCGGCACTGGTTATATTCTCTCCATTATCATGGCCATCCTTGCAGACACATTCTTCGGCCGATTCAAAACCCTTCTCATTTCAGCTTGCTTCGAGTTCGTCGGGCTAGGATTACTAACTGTGCAAGCTCACTATCCAAGTCTCCAGCCACCGGTCTGTAACGTGTTTGATCCAACTTCTTCTTGCGAGAAAGTTACTGGTGGCAATGCTGCTTTGCTTTTCATTGCTCTCTACATATTGGCAGCAGGAGCTGCAGGAGTCAAAGCTACCTTGCCATCACATGGTGCAGATCAATTTGATGAAAAAGATCCAAAAGAGACTAGGCATATGTCGAGCTTCTTCAACCTGCTCTTACTAGCAGTATGTCTGGGTGGTGTGGTCAGCCTAACACTAATTGTGTGGATCCAAGACAATAAAGGATGGGATTGGGGATTTGGGATTTCTACAATCGCCATGTTCTTGGGTGTCGTCGTCTTCTTCTCAGGATTGCCTATGTACCGGATACAAGCTATTCGAGGAACTAGTGCAATAATCGAAATTATACAGGTGTATGTTGCCGCGGTCCGCAACAGAAATCTCACCCTTCCTGAGAACCCTTCAGATCTCTATGAGATCGAGGCAGACAAGGAAGCTTCACTTGAAGAAGAATTCCTACCTCACAGACCCATTTTCGGCTTCTTGGACAAGGCAGCAATTAAACGAGGGCAAAATGATGACGCCGAATCTCTAAACTCATGGAAGCTTTGCAGAGTCACCCAAGTCGAAAATGCAAAGATAATACTAGGCATGTACTTCATTTTCGGAATCGCTGACATGTTCACCTACGTCGGTCTTCTTGAGTTTTTCTACTCGGAGGCACCAAAAGGGCTCAAGTCCATCTCGACTTGCTTCCTATGGAGCTCAATGGCACTCGGGTACTTCTTCAGCACAGTGTTGGTTAAGATTGTGAATGCGGCAACACATGGGAGCAATGGGGGTTGGCTGGCTGGGAACAACATCAACAGGAACCACTTGAACCTTTTCTATTGGTTGCTTTCAGGGATGAGCTTCTTGAACTTCTTCCTCTATCTGTTTGTTTCAAGGAGGTATAAGTATAGACCAGGGAGTCCCATGCTGGTCTCAAAAGATGACAAGAAGGAGGATGAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

458

Amino Acids

50.97

Weight (kDa)

6.33

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 97 - 348 9.3e-41 POT family
PTR2 PF00854 338 - 396 5.4e-07 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016782)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g06770
malus_domestica MD05G1293900.v1.1 MD10G1271800.v1.1
prunus_persica Prupe.4G070100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0012361
rosa_laevigata RLG00000031907
rosa_multiflora Rmu_sc0003469.1_g000017
rosa_roxburghii Rroxscaffold_1G00064300
rosa_rugosa Rorug05G0000700
rosa_samantha Rh5AG094900 Rh5BG091200 Rh5CG104200 Rh5DG090600
rosa_wichuraiana Rw5G008300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1329
Acc36I ACCTGC 1 cut(s) 579
AccB1I GGYRCC 1 cut(s) 1081
AccB7I CCANNNNNTGG 1 cut(s) 506
AccII CGCG 1 cut(s) 785
AciI CCGC 6 cut(s) 149, 204, 783, 785, 790, 1178
AclWI GGATC 4 cut(s) 389, 527, 622, 635
AcoI YGGCCR 2 cut(s) 258, 283
AcsI RAATTY 1 cut(s) 872
AcuI CTGAAG 2 cut(s) 807, 1132
AcyI GRCGYC 1 cut(s) 942
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 3 cut(s) 722, 1016, 1142
AfiI CCNNNNNNNGG 3 cut(s) 376, 506, 896
AflIII ACRYGT 2 cut(s) 387, 1038
AgeI ACCGGT 1 cut(s) 376
AgsI TTSAA 9 cut(s) 32, 172, 191, 292, 568, 866, 1240, 1279, 1305
AhlI ACTAGT 1 cut(s) 746
AjnI CCWGG 1 cut(s) 1324
AjuI GAANNNNNNNTTGG 2 cut(s) 28, 60
Alw21I GWGCWC 2 cut(s) 1127, 1374
Alw26I GTCTC 3 cut(s) 371, 536, 1348
AlwI GGATC 4 cut(s) 389, 527, 622, 635
AlwNI CAGNNNCTG 1 cut(s) 476
Ama87I CYCGRG 1 cut(s) 1136
AoxI GGCC 2 cut(s) 258, 283
ApeKI GCWGC 4 cut(s) 434, 467, 476, 914
ApoI RAATTY 1 cut(s) 872
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AsiGI ACCGGT 1 cut(s) 376
Asp700I GAANNNNTTC 2 cut(s) 1244, 1283
AspS9I GGNCC 1 cut(s) 787
AsuHPI GGTGA 5 cut(s) 163, 190, 796, 970, 1036
AvaI CYCGRG 1 cut(s) 1136
AvaII GGWCC 1 cut(s) 787
BalI TGGCCA 1 cut(s) 260
BamHI GGATCC 1 cut(s) 627
BanI GGYRCC 1 cut(s) 1081
BanII GRGCYC 3 cut(s) 1095, 1127, 1374
BarI GAAGNNNNNNTAC 2 cut(s) 861, 893
BbsI GAAGAC 2 cut(s) 691, 1049
Bbv12I GWGCWC 2 cut(s) 1127, 1374
BbvI GCAGC 4 cut(s) 421, 463, 479, 926
BccI CCATC 4 cut(s) 269, 508, 639, 1109
BciT130I CCWGG 1 cut(s) 1326
BciVI GTATCC 1 cut(s) 720
BcoDI GTCTC 3 cut(s) 371, 536, 1348
BcuI ACTAGT 1 cut(s) 746
BfaI CTAG 5 cut(s) 332, 546, 581, 747, 1007
BfmI CTRYAG 1 cut(s) 477
BfuAI ACCTGC 1 cut(s) 579
BfuI GTATCC 1 cut(s) 720
BglII AGATCT 1 cut(s) 826
BisI GCNGC 7 cut(s) 204, 435, 468, 477, 783, 915, 1179
BlsI GCNGC 7 cut(s) 205, 436, 469, 478, 784, 916, 1180
Bme1390I CCNGG 1 cut(s) 1326
Bme18I GGWCC 1 cut(s) 787
BmeT110I CYCGRG 1 cut(s) 1136
BmgT120I GGNCC 1 cut(s) 787
BmiI GGNNCC 3 cut(s) 629, 1083, 1232
BmrFI CCNGG 1 cut(s) 1326
BmsI GCATC 2 cut(s) 171, 190
BpiI GAAGAC 2 cut(s) 691, 1049
BpmI CTGGAG 1 cut(s) 353
BpuEI CTTGAG 2 cut(s) 1079, 1082
BsaHI GRCGYC 1 cut(s) 942
BsaI GGTCTC 1 cut(s) 1348
BsaJI CCNNGG 2 cut(s) 783, 1325
BsaWI WCCGGW 2 cut(s) 376, 723
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 506, 896
Bse118I RCCGGY 2 cut(s) 227, 376
Bse1I ACTGG 2 cut(s) 238, 427
Bse3DI GCAATG 3 cut(s) 436, 447, 1201
BseBI CCWGG 1 cut(s) 1326
BseDI CCNNGG 2 cut(s) 783, 1325
BseGI GGATG 4 cut(s) 261, 650, 1272, 1372
BseLI CCNNNNNNNGG 3 cut(s) 376, 506, 896
BseMI GCAATG 3 cut(s) 436, 447, 1201
BseMII CTCAG 2 cut(s) 720, 804
BseNI ACTGG 2 cut(s) 238, 427
BseX3I CGGCCG 1 cut(s) 283
BseXI GCAGC 4 cut(s) 421, 463, 479, 926
BseYI CCCAGC 1 cut(s) 1211
BsgI GTGCAG 1 cut(s) 531
Bsh1236I CGCG 1 cut(s) 785
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 2 cut(s) 260, 285
BshNI GGYRCC 1 cut(s) 1081
BshTI ACCGGT 1 cut(s) 376
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 2 cut(s) 1127, 1374
BsiHKCI CYCGRG 1 cut(s) 1136
BsiSI CCGG 3 cut(s) 228, 377, 724
BslFI GGGAC 1 cut(s) 1317
BslI CCNNNNNNNGG 3 cut(s) 376, 506, 896
BsmAI GTCTC 3 cut(s) 371, 536, 1348
BsmFI GGGAC 1 cut(s) 1317
BsmI GAATGC 1 cut(s) 1180
BsnI GGCC 2 cut(s) 260, 285
Bso31I GGTCTC 1 cut(s) 1348
BsoBI CYCGRG 1 cut(s) 1136
Bsp1286I GDGCHC 3 cut(s) 1095, 1127, 1374
Bsp143I GATC 6 cut(s) 394, 514, 532, 627, 826, 837
BspACI CCGC 6 cut(s) 149, 204, 783, 785, 790, 1178
BspANI GGCC 2 cut(s) 260, 285
BspCNI CTCAG 2 cut(s) 719, 805
BspFNI CGCG 1 cut(s) 785
BspLI GGNNCC 3 cut(s) 629, 1083, 1232
BspMAI CTGCAG 1 cut(s) 481
BspMI ACCTGC 1 cut(s) 579
BspPI GGATC 4 cut(s) 389, 527, 622, 635
BspT107I GGYRCC 1 cut(s) 1081
BspTNI GGTCTC 1 cut(s) 1348
BsrDI GCAATG 3 cut(s) 436, 447, 1201
BsrFI RCCGGY 2 cut(s) 227, 376
BsrI ACTGG 2 cut(s) 238, 427
BssAI RCCGGY 2 cut(s) 227, 376
BssECI CCNNGG 2 cut(s) 783, 1325
BssMI GATC 6 cut(s) 394, 514, 532, 627, 826, 837
BssNI GRCGYC 1 cut(s) 942
Bst2UI CCWGG 1 cut(s) 1326
Bst4CI ACNGT 2 cut(s) 346, 1156
BstACI GRCGYC 1 cut(s) 942
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 4 cut(s) 229, 313, 351, 1209
BstDEI CTNAG 2 cut(s) 706, 813
BstDSI CCRYGG 1 cut(s) 783
BstF5I GGATG 4 cut(s) 261, 650, 1272, 1372
BstFNI CGCG 1 cut(s) 785
BstKTI GATC 6 cut(s) 397, 517, 535, 630, 829, 840
BstMAI GTCTC 3 cut(s) 371, 536, 1348
BstMBI GATC 6 cut(s) 394, 514, 532, 627, 826, 837
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 4 cut(s) 140, 266, 473, 476
BstNI CCWGG 1 cut(s) 1326
BstNSI RCATGY 2 cut(s) 1015, 1042
BstSCI CCNGG 1 cut(s) 1324
BstSFI CTRYAG 1 cut(s) 477
BstUI CGCG 1 cut(s) 785
BstV1I GCAGC 4 cut(s) 421, 463, 479, 926
BstV2I GAAGAC 2 cut(s) 691, 1049
BstX2I RGATCY 3 cut(s) 532, 627, 826
BstXI CCANNNNNNTGG 2 cut(s) 256, 685
BstYI RGATCY 3 cut(s) 532, 627, 826
BstZI CGGCCG 1 cut(s) 283
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 2 cut(s) 260, 285
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 4 cut(s) 261, 650, 1272, 1372
BtsIMutI CAGTG 2 cut(s) 231, 1161
BveI ACCTGC 1 cut(s) 579
Cac8I GCNNGC 4 cut(s) 229, 313, 351, 1209
CaiI CAGNNNCTG 1 cut(s) 476
Cfr10I RCCGGY 2 cut(s) 227, 376
Cfr13I GGNCC 1 cut(s) 787
Cfr42I CCGCGG 1 cut(s) 786
CpoI CGGWCCG 1 cut(s) 787
CseI GACGC 1 cut(s) 950
Csp6I GTAC 3 cut(s) 721, 1015, 1141
CspAI ACCGGT 1 cut(s) 376
CspI CGGWCCG 1 cut(s) 787
CviQI GTAC 3 cut(s) 721, 1015, 1141
DdeI CTNAG 2 cut(s) 706, 813
DpnI GATC 6 cut(s) 396, 516, 534, 629, 828, 839
DpnII GATC 6 cut(s) 394, 514, 532, 627, 826, 837
DraIII CACNNNGTG 1 cut(s) 509
DrdI GACNNNNNNGTC 1 cut(s) 1329
DseDI GACNNNNNNGTC 1 cut(s) 1329
EaeI YGGCCR 2 cut(s) 258, 283
EagI CGGCCG 1 cut(s) 283
Ecl136II GAGCTC 2 cut(s) 1125, 1372
EclXI CGGCCG 1 cut(s) 283
Eco24I GRGCYC 3 cut(s) 1095, 1127, 1374
Eco31I GGTCTC 1 cut(s) 1348
Eco47I GGWCC 1 cut(s) 787
Eco52I CGGCCG 1 cut(s) 283
Eco53kI GAGCTC 2 cut(s) 1125, 1372
Eco57I CTGAAG 2 cut(s) 807, 1132
Eco88I CYCGRG 1 cut(s) 1136
EcoICRI GAGCTC 2 cut(s) 1125, 1372
EcoRI GAATTC 1 cut(s) 872
EcoRII CCWGG 1 cut(s) 1324
EcoT22I ATGCAT 1 cut(s) 186
EcoT38I GRGCYC 3 cut(s) 1095, 1127, 1374
FalI AAGNNNNNCTT 2 cut(s) 846, 878
FaqI GGGAC 1 cut(s) 1317
FauNDI CATATG 1 cut(s) 552
Fnu4HI GCNGC 7 cut(s) 204, 435, 468, 477, 783, 915, 1179
FokI GGATG 3 cut(s) 248, 657, 1279
FriOI GRGCYC 3 cut(s) 1095, 1127, 1374
Fsp4HI GCNGC 7 cut(s) 204, 435, 468, 477, 783, 915, 1179
FspBI CTAG 5 cut(s) 332, 546, 581, 747, 1007
GluI GCNGC 7 cut(s) 204, 435, 468, 477, 783, 915, 1179
GsaI CCCAGC 1 cut(s) 1215
GsuI CTGGAG 1 cut(s) 353
HaeIII GGCC 2 cut(s) 260, 285
HapII CCGG 3 cut(s) 228, 377, 724
HgaI GACGC 1 cut(s) 950
Hin1I GRCGYC 1 cut(s) 942
HindIII AAGCTT 2 cut(s) 855, 965
HinfI GANTC 6 cut(s) 288, 483, 947, 975, 1029, 1330
HpaII CCGG 3 cut(s) 228, 377, 724
HphI GGTGA 5 cut(s) 163, 190, 796, 970, 1036
Hpy166II GTNNAC 2 cut(s) 154, 1044
Hpy188I TCNGA 4 cut(s) 199, 826, 1028, 1078
Hpy188III TCNNGA 5 cut(s) 708, 812, 1061, 1105, 1276
Hpy8I GTNNAC 2 cut(s) 154, 1044
Hpy99I CGWCG 3 cut(s) 329, 698, 1055
HpyAV CCTTC 8 cut(s) 10, 26, 43, 48, 308, 818, 831, 1354
HpyCH4III ACNGT 2 cut(s) 346, 1156
HpyCH4IV ACGT 2 cut(s) 387, 1050
HpyCH4V TGCA 8 cut(s) 184, 269, 349, 479, 512, 752, 972, 995
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 266, 473, 476
HpyF3I CTNAG 2 cut(s) 706, 813
HpySE526I ACGT 2 cut(s) 387, 1050
Hsp92I GRCGYC 1 cut(s) 942
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
KspI CCGCGG 1 cut(s) 786
Kzo9I GATC 6 cut(s) 394, 514, 532, 627, 826, 837
LmnI GCTCC 3 cut(s) 473, 1122, 1191
Lsp1109I GCAGC 4 cut(s) 421, 463, 479, 926
LweI GCATC 2 cut(s) 171, 190
MaeI CTAG 5 cut(s) 332, 546, 581, 747, 1007
MaeII ACGT 2 cut(s) 387, 1050
MaeIII GTNAC 4 cut(s) 160, 383, 418, 976
MalI GATC 6 cut(s) 396, 516, 534, 629, 828, 839
MboI GATC 6 cut(s) 394, 514, 532, 627, 826, 837
MflI RGATCY 3 cut(s) 532, 627, 826
MhlI GDGCHC 3 cut(s) 1095, 1127, 1374
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 5 cut(s) 518, 618, 762, 872, 918
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 3 cut(s) 492, 984, 1339
MmeI TCCRAC 1 cut(s) 422
MnlI CCTC 9 cut(s) 74, 734, 835, 891, 920, 1072, 1298, 1302, 1357
Mox20I TGGCCA 1 cut(s) 260
Mph1103I ATGCAT 1 cut(s) 186
MroNI GCCGGC 1 cut(s) 227
MroXI GAANNNNTTC 2 cut(s) 1244, 1283
MscI TGGCCA 1 cut(s) 260
MseI TTAA 2 cut(s) 921, 1164
MslI CAYNNNNRTG 2 cut(s) 75, 254
Msp20I TGGCCA 1 cut(s) 260
MspA1I CMGCKG 2 cut(s) 62, 785
MspI CCGG 3 cut(s) 228, 377, 724
MspR9I CCNGG 1 cut(s) 1326
Mva1269I GAATGC 1 cut(s) 1180
MvaI CCWGG 1 cut(s) 1326
MvnI CGCG 1 cut(s) 785
MwoI GCNNNNNNNGC 4 cut(s) 140, 266, 473, 476
NaeI GCCGGC 1 cut(s) 229
NdeI CATATG 1 cut(s) 552
NdeII GATC 6 cut(s) 394, 514, 532, 627, 826, 837
NgoMIV GCCGGC 1 cut(s) 227
NlaIV GGNNCC 3 cut(s) 629, 1083, 1232
NmuCI GTSAC 1 cut(s) 976
NsiI ATGCAT 1 cut(s) 186
NspI RCATGY 2 cut(s) 1015, 1042
PciI ACATGT 1 cut(s) 1038
PctI GAATGC 1 cut(s) 1180
PdiI GCCGGC 1 cut(s) 229
PdmI GAANNNNTTC 2 cut(s) 1244, 1283
PfeI GAWTC 3 cut(s) 288, 947, 1029
PflMI CCANNNNNTGG 1 cut(s) 506
PinAI ACCGGT 1 cut(s) 376
PkrI GCNGC 7 cut(s) 205, 436, 469, 478, 784, 916, 1180
PleI GAGTC 3 cut(s) 491, 983, 1338
PpsI GAGTC 3 cut(s) 491, 983, 1338
PscI ACATGT 1 cut(s) 1038
Psp124BI GAGCTC 2 cut(s) 1127, 1374
Psp6I CCWGG 1 cut(s) 1324
PspFI CCCAGC 1 cut(s) 1211
PspGI CCWGG 1 cut(s) 1324
PspN4I GGNNCC 3 cut(s) 629, 1083, 1232
PspPI GGNCC 1 cut(s) 787
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 1 cut(s) 476
PsuI RGATCY 3 cut(s) 532, 627, 826
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 3 cut(s) 722, 1016, 1142
RsaNI GTAC 3 cut(s) 721, 1015, 1141
RseI CAYNNNNRTG 2 cut(s) 75, 254
Rsr2I CGGWCCG 1 cut(s) 787
RsrII CGGWCCG 1 cut(s) 787
SacI GAGCTC 2 cut(s) 1127, 1374
SacII CCGCGG 1 cut(s) 786
SaqAI TTAA 2 cut(s) 921, 1164
SatI GCNGC 7 cut(s) 204, 435, 468, 477, 783, 915, 1179
Sau3AI GATC 6 cut(s) 394, 514, 532, 627, 826, 837
Sau96I GGNCC 1 cut(s) 787
SchI GAGTC 3 cut(s) 492, 984, 1339
ScrFI CCNGG 1 cut(s) 1326
SduI GDGCHC 3 cut(s) 1095, 1127, 1374
SfaNI GCATC 2 cut(s) 171, 190
SfcI CTRYAG 1 cut(s) 477
Sfr303I CCGCGG 1 cut(s) 786
SgrBI CCGCGG 1 cut(s) 786
SinI GGWCC 1 cut(s) 787
SmiMI CAYNNNNRTG 2 cut(s) 75, 254
SmlI CTYRAG 2 cut(s) 1061, 1094
SmoI CTYRAG 2 cut(s) 1061, 1094
SpeI ACTAGT 1 cut(s) 746
Sse9I AATT 5 cut(s) 518, 618, 762, 872, 918
SsiI CCGC 6 cut(s) 149, 204, 783, 785, 790, 1178
SspMI CTAG 5 cut(s) 332, 546, 581, 747, 1007
SstI GAGCTC 2 cut(s) 1127, 1374
StyD4I CCNGG 1 cut(s) 1324
TaaI ACNGT 2 cut(s) 346, 1156
TaiI ACGT 2 cut(s) 390, 1053
TaqI TCGA 7 cut(s) 318, 557, 739, 759, 840, 987, 1106
TaqII GACCGA 1 cut(s) 1043
TasI AATT 5 cut(s) 518, 618, 762, 872, 918
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 3 cut(s) 206, 785, 1181
TfiI GAWTC 3 cut(s) 288, 947, 1029
Tru1I TTAA 2 cut(s) 921, 1164
Tru9I TTAA 2 cut(s) 921, 1164
TscAI CASTG 2 cut(s) 238, 1161
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 4 cut(s) 434, 467, 476, 914
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 3 cut(s) 436, 540, 1009
TspRI CASTG 2 cut(s) 238, 1161
Van91I CCANNNNNTGG 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 1 cut(s) 872
XceI RCATGY 2 cut(s) 1015, 1042
XmnI GAANNNNTTC 2 cut(s) 1244, 1283
XspI CTAG 5 cut(s) 332, 546, 581, 747, 1007
Zsp2I ATGCAT 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.