Rroxscaffold_2G00111960

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018629
Physical Location & Seq
Forward (+)
36849191 .. 36852178
2988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00111960.1

Sequence Viewer

Length: 867 bp
ATGGATGATTTGCTCAAAAAGCGAGCCTTAGGGTTCGAGTCTAGTGCAGCGGCAACTCTCCGGATTGCTGTTTGTTTTAGCCCAGCGAGAAAGTCGCGGGTTGCGGTAAAGCCGGGTGTGGGGCAGCGTGGGTTTGGTGGCGGCAGAAAGTCGACCGATTCCGTCTTGTCTTGGTGGTGCAACGCGGAGGCAAAACGTGGAGAGAGCTTTCAATTTGGATCTCTTGATCCGGTCTCCAATTCTAGGAGTGGCACGGTCGACCGCCGGAGGGCTGGTGGTAGCAGAGGCAGCCCGGAATCGAGGGCTAGGTTGGCCTGGGTTTGGGCTTGGGTTGTTGGGCCTGGATTCAAACCTGGAATGAGGGATGTTTGGGCTTTTGGACCTAGATTTGAGCTTAAATTTGGGCTGGTTGAGTCTGCTGCAATGGACAAGAAGAGCAAGACCAACCTGTTGCCGAGGTTGTTGACTCTGAAGAGTTTGAGGACGCAATCACTTGATGTGTTGCTTAAACAATTCAAGCTAAAGCTATGTGTGGACTTGGAAGAGCTCTCTCTGATAGGAGATCTTTTGAAATCGGTACCGGTGTTGGTCCAGGGTTGGGAGGTGGCGGTGGTGAAGGTTTTGGGGCATAATCATTATCTTCGTGCTTCACAAACCAGGCTAGAGGCGGCGTGGATGATGGCTTTGGTTTTGTCCTCGGAGACCAGGGACACCCCAAACGGCGTCACCATTAAGGTGAAGGCCAACGTGATCGAAGTCGCAGGTCTGTGCCGGAAGTTCAACATAAACTCCGAGCAAACCAATTTCGACTTCGAACTCGTCACTGAGGAGTCTACCGGCAAGAAGAATCAAAAGATGGAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6

Protein Analysis

288

Amino Acids

31.79

Weight (kDa)

9.94

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018629)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0303521 RchiOBHm_Chr7g0199361
rosa_multiflora Rmu_sc0004014.1_g000002
rosa_roxburghii Rroxscaffold_2G00111960
rosa_rugosa Rorug01G0281700 Rorug03G0298500 Rorug05G0123200
rosa_samantha Rh2DG188100 Rh3BG201400 Rh7DG181500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 752
Acc65I GGTACC 1 cut(s) 577
AccB1I GGYRCC 1 cut(s) 577
AccI GTMKAC 3 cut(s) 152, 258, 833
AccII CGCG 2 cut(s) 97, 185
AccIII TCCGGA 1 cut(s) 60
AciI CCGC 8 cut(s) 50, 97, 104, 141, 185, 262, 608, 668
AclWI GGATC 2 cut(s) 221, 226
AcsI RAATTY 1 cut(s) 398
AcuI CTGAAG 1 cut(s) 491
AcyI GRCGYC 1 cut(s) 723
AfaI GTAC 1 cut(s) 579
AfiI CCNNNNNNNGG 5 cut(s) 119, 243, 268, 321, 598
AgeI ACCGGT 1 cut(s) 580
AgsI TTSAA 5 cut(s) 212, 349, 517, 571, 781
AjnI CCWGG 6 cut(s) 314, 340, 352, 591, 656, 704
AjuI GAANNNNNNNTTGG 2 cut(s) 794, 826
AluBI AGCT 5 cut(s) 207, 394, 520, 526, 547
AluI AGCT 5 cut(s) 207, 394, 520, 526, 547
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 2 cut(s) 238, 695
AlwI GGATC 2 cut(s) 221, 226
Aor13HI TCCGGA 1 cut(s) 60
AoxI GGCC 4 cut(s) 312, 338, 741, 861
ApeKI GCWGC 4 cut(s) 47, 124, 288, 419
ApoI RAATTY 1 cut(s) 398
AsiGI ACCGGT 1 cut(s) 580
Asp718I GGTACC 1 cut(s) 577
AspS9I GGNCC 3 cut(s) 338, 380, 589
AsuC2I CCSGG 2 cut(s) 114, 293
AsuHPI GGTGA 3 cut(s) 625, 718, 748
AsuII TTCGAA 1 cut(s) 813
AvaII GGWCC 2 cut(s) 380, 589
AxyI CCTNAGG 1 cut(s) 28
BaeI ACNNNNGTAYC 2 cut(s) 569, 602
BanI GGYRCC 1 cut(s) 577
BanII GRGCYC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 4 cut(s) 59, 136, 300, 406
BccI CCATC 2 cut(s) 673, 850
BceAI ACGGC 1 cut(s) 736
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 6 cut(s) 316, 342, 354, 593, 658, 706
BcnI CCSGG 2 cut(s) 114, 293
BcoDI GTCTC 2 cut(s) 238, 695
BfaI CTAG 5 cut(s) 42, 243, 306, 384, 662
BfuAI ACCTGC 1 cut(s) 752
BglII AGATCT 1 cut(s) 562
BisI GCNGC 7 cut(s) 48, 51, 125, 142, 289, 420, 669
BlsI GCNGC 7 cut(s) 49, 52, 126, 143, 290, 421, 670
Bme1390I CCNGG 8 cut(s) 114, 293, 316, 342, 354, 593, 658, 706
Bme18I GGWCC 2 cut(s) 380, 589
BmgT120I GGNCC 3 cut(s) 338, 380, 589
BmiI GGNNCC 1 cut(s) 579
BmrFI CCNGG 8 cut(s) 114, 293, 316, 342, 354, 593, 658, 706
Bpu14I TTCGAA 1 cut(s) 813
BpuMI CCSGG 2 cut(s) 114, 293
BsaHI GRCGYC 1 cut(s) 723
BsaI GGTCTC 2 cut(s) 238, 695
BsaJI CCNNGG 5 cut(s) 315, 455, 592, 696, 705
BsaWI WCCGGW 3 cut(s) 60, 229, 580
BsaXI ACNNNNNCTCC 4 cut(s) 259, 289, 773, 803
Bsc4I CCNNNNNNNGG 5 cut(s) 119, 243, 268, 321, 598
Bse118I RCCGGY 2 cut(s) 580, 836
Bse21I CCTNAGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 60
BseBI CCWGG 6 cut(s) 316, 342, 354, 593, 658, 706
BseDI CCNNGG 5 cut(s) 315, 455, 592, 696, 705
BseGI GGATG 3 cut(s) 10, 370, 681
BseLI CCNNNNNNNGG 5 cut(s) 119, 243, 268, 321, 598
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 816
BseRI GAGGAG 1 cut(s) 842
BseXI GCAGC 4 cut(s) 59, 136, 300, 406
BseYI CCCAGC 1 cut(s) 82
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 97, 185
Bsh1285I CGRYCG 3 cut(s) 156, 258, 262
BshFI GGCC 4 cut(s) 314, 340, 743, 863
BshNI GGYRCC 1 cut(s) 577
BshTI ACCGGT 1 cut(s) 580
BsiEI CGRYCG 3 cut(s) 156, 258, 262
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 8 cut(s) 61, 113, 230, 265, 293, 581, 772, 837
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 5 cut(s) 119, 243, 268, 321, 598
BsmAI GTCTC 2 cut(s) 238, 695
BsmFI GGGAC 1 cut(s) 722
BsnI GGCC 4 cut(s) 314, 340, 743, 863
Bso31I GGTCTC 2 cut(s) 238, 695
Bsp119I TTCGAA 1 cut(s) 813
Bsp1286I GDGCHC 1 cut(s) 549
Bsp13I TCCGGA 1 cut(s) 60
Bsp143I GATC 4 cut(s) 218, 226, 562, 750
BspACI CCGC 8 cut(s) 50, 97, 104, 141, 185, 262, 608, 668
BspANI GGCC 4 cut(s) 314, 340, 743, 863
BspCNI CTCAG 1 cut(s) 817
BspEI TCCGGA 1 cut(s) 60
BspFNI CGCG 2 cut(s) 97, 185
BspLI GGNNCC 1 cut(s) 579
BspMI ACCTGC 1 cut(s) 752
BspPI GGATC 2 cut(s) 221, 226
BspQI GCTCTTC 2 cut(s) 428, 537
BspT104I TTCGAA 1 cut(s) 813
BspT107I GGYRCC 1 cut(s) 577
BspTNI GGTCTC 2 cut(s) 238, 695
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 2 cut(s) 580, 836
BssAI RCCGGY 2 cut(s) 580, 836
BssECI CCNNGG 5 cut(s) 315, 455, 592, 696, 705
BssMI GATC 4 cut(s) 218, 226, 562, 750
BssNI GRCGYC 1 cut(s) 723
Bst2UI CCWGG 6 cut(s) 316, 342, 354, 593, 658, 706
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 3 cut(s) 428, 467, 537
BstACI GRCGYC 1 cut(s) 723
BstBI TTCGAA 1 cut(s) 813
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 2 cut(s) 28, 825
BstF5I GGATG 3 cut(s) 10, 370, 681
BstFNI CGCG 2 cut(s) 97, 185
BstKTI GATC 4 cut(s) 221, 229, 565, 753
BstMAI GTCTC 2 cut(s) 238, 695
BstMBI GATC 4 cut(s) 218, 226, 562, 750
BstMCI CGRYCG 3 cut(s) 156, 258, 262
BstMWI GCNNNNNNNGC 3 cut(s) 19, 288, 311
BstNI CCWGG 6 cut(s) 316, 342, 354, 593, 658, 706
BstSCI CCNGG 8 cut(s) 112, 291, 314, 340, 352, 591, 656, 704
BstUI CGCG 2 cut(s) 97, 185
BstV1I GCAGC 4 cut(s) 59, 136, 300, 406
BstX2I RGATCY 2 cut(s) 218, 562
BstYI RGATCY 2 cut(s) 218, 562
Bsu36I CCTNAGG 1 cut(s) 28
BsuRI GGCC 4 cut(s) 314, 340, 743, 863
BtsCI GGATG 3 cut(s) 10, 370, 681
BtsIMutI CAGTG 1 cut(s) 822
BveI ACCTGC 1 cut(s) 752
Cac8I GCNNGC 1 cut(s) 24
Cfr10I RCCGGY 2 cut(s) 580, 836
Cfr13I GGNCC 3 cut(s) 338, 380, 589
CseI GACGC 2 cut(s) 493, 712
Csp6I GTAC 1 cut(s) 578
CspAI ACCGGT 1 cut(s) 580
CviQI GTAC 1 cut(s) 578
DdeI CTNAG 2 cut(s) 28, 825
DpnI GATC 4 cut(s) 220, 228, 564, 752
DpnII GATC 4 cut(s) 218, 226, 562, 750
Eam1104I CTCTTC 3 cut(s) 428, 467, 537
EarI CTCTTC 3 cut(s) 428, 467, 537
Ecl136II GAGCTC 1 cut(s) 547
Eco147I AGGCCT 1 cut(s) 863
Eco24I GRGCYC 1 cut(s) 549
Eco31I GGTCTC 2 cut(s) 238, 695
Eco47I GGWCC 2 cut(s) 380, 589
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 491
Eco81I CCTNAGG 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 547
EcoRII CCWGG 6 cut(s) 314, 340, 352, 591, 656, 704
EcoT38I GRGCYC 1 cut(s) 549
FaiI YATR 3 cut(s) 529, 630, 785
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 1 cut(s) 722
FauI CCCGC 1 cut(s) 90
FblI GTMKAC 3 cut(s) 152, 258, 833
Fnu4HI GCNGC 7 cut(s) 48, 51, 125, 142, 289, 420, 669
FokI GGATG 3 cut(s) 17, 377, 688
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 7 cut(s) 48, 51, 125, 142, 289, 420, 669
FspBI CTAG 5 cut(s) 42, 243, 306, 384, 662
GluI GCNGC 7 cut(s) 48, 51, 125, 142, 289, 420, 669
GsaI CCCAGC 1 cut(s) 86
HaeIII GGCC 4 cut(s) 314, 340, 743, 863
HapII CCGG 8 cut(s) 61, 113, 230, 265, 293, 581, 772, 837
HgaI GACGC 2 cut(s) 493, 712
Hin1I GRCGYC 1 cut(s) 723
HincII GTYRAC 3 cut(s) 153, 259, 465
HindII GTYRAC 3 cut(s) 153, 259, 465
HinfI GANTC 8 cut(s) 38, 158, 296, 345, 413, 466, 830, 847
HpaII CCGG 8 cut(s) 61, 113, 230, 265, 293, 581, 772, 837
HphI GGTGA 3 cut(s) 625, 718, 748
Hpy166II GTNNAC 5 cut(s) 153, 259, 465, 535, 834
Hpy188I TCNGA 4 cut(s) 471, 555, 700, 793
Hpy188III TCNNGA 2 cut(s) 61, 224
Hpy8I GTNNAC 5 cut(s) 153, 259, 465, 535, 834
HpyAV CCTTC 2 cut(s) 610, 733
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 2 cut(s) 196, 747
HpyCH4V TGCA 3 cut(s) 47, 180, 422
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 288, 311
HpyF3I CTNAG 2 cut(s) 28, 825
HpySE526I ACGT 2 cut(s) 196, 747
Hsp92I GRCGYC 1 cut(s) 723
Kpn2I TCCGGA 1 cut(s) 60
KpnI GGTACC 1 cut(s) 581
Kzo9I GATC 4 cut(s) 218, 226, 562, 750
LguI GCTCTTC 2 cut(s) 428, 537
Lsp1109I GCAGC 4 cut(s) 59, 136, 300, 406
MaeI CTAG 5 cut(s) 42, 243, 306, 384, 662
MaeII ACGT 2 cut(s) 196, 747
MaeIII GTNAC 2 cut(s) 724, 820
MalI GATC 4 cut(s) 220, 228, 564, 752
MboI GATC 4 cut(s) 218, 226, 562, 750
MboII GAAGA 5 cut(s) 445, 484, 554, 632, 856
MflI RGATCY 2 cut(s) 218, 562
MhlI GDGCHC 1 cut(s) 549
MluCI AATT 5 cut(s) 212, 238, 398, 512, 802
MlyI GAGTC 4 cut(s) 47, 422, 460, 839
MroI TCCGGA 1 cut(s) 60
MseI TTAA 3 cut(s) 396, 507, 732
MslI CAYNNNNRTG 1 cut(s) 734
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 8 cut(s) 61, 113, 230, 265, 293, 581, 772, 837
MspR9I CCNGG 8 cut(s) 114, 293, 316, 342, 354, 593, 658, 706
MvaI CCWGG 6 cut(s) 316, 342, 354, 593, 658, 706
MvnI CGCG 2 cut(s) 97, 185
MwoI GCNNNNNNNGC 3 cut(s) 19, 288, 311
NciI CCSGG 2 cut(s) 114, 293
NdeII GATC 4 cut(s) 218, 226, 562, 750
NlaIV GGNNCC 1 cut(s) 579
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 2 cut(s) 724, 820
NspV TTCGAA 1 cut(s) 813
PceI AGGCCT 1 cut(s) 863
PciSI GCTCTTC 2 cut(s) 428, 537
PfeI GAWTC 4 cut(s) 158, 296, 345, 847
PinAI ACCGGT 1 cut(s) 580
PkrI GCNGC 7 cut(s) 49, 52, 126, 143, 290, 421, 670
PleI GAGTC 4 cut(s) 46, 421, 460, 838
PpsI GAGTC 4 cut(s) 46, 421, 460, 838
Psp124BI GAGCTC 1 cut(s) 549
Psp6I CCWGG 6 cut(s) 314, 340, 352, 591, 656, 704
PspFI CCCAGC 1 cut(s) 82
PspGI CCWGG 6 cut(s) 314, 340, 352, 591, 656, 704
PspN4I GGNNCC 1 cut(s) 579
PspPI GGNCC 3 cut(s) 338, 380, 589
PsuI RGATCY 2 cut(s) 218, 562
RsaI GTAC 1 cut(s) 579
RsaNI GTAC 1 cut(s) 578
RseI CAYNNNNRTG 1 cut(s) 734
SacI GAGCTC 1 cut(s) 549
SalI GTCGAC 2 cut(s) 151, 257
SapI GCTCTTC 2 cut(s) 428, 537
SaqAI TTAA 3 cut(s) 396, 507, 732
SatI GCNGC 7 cut(s) 48, 51, 125, 142, 289, 420, 669
Sau3AI GATC 4 cut(s) 218, 226, 562, 750
Sau96I GGNCC 3 cut(s) 338, 380, 589
SchI GAGTC 4 cut(s) 47, 422, 460, 839
ScrFI CCNGG 8 cut(s) 114, 293, 316, 342, 354, 593, 658, 706
SduI GDGCHC 1 cut(s) 549
SfuI TTCGAA 1 cut(s) 813
SinI GGWCC 2 cut(s) 380, 589
SmiMI CAYNNNNRTG 1 cut(s) 734
Sse9I AATT 5 cut(s) 212, 238, 398, 512, 802
SseBI AGGCCT 1 cut(s) 863
SsiI CCGC 8 cut(s) 50, 97, 104, 141, 185, 262, 608, 668
SspMI CTAG 5 cut(s) 42, 243, 306, 384, 662
SstI GAGCTC 1 cut(s) 549
StuI AGGCCT 1 cut(s) 863
StyD4I CCNGG 8 cut(s) 112, 291, 314, 340, 352, 591, 656, 704
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 2 cut(s) 199, 750
TaqI TCGA 7 cut(s) 36, 152, 258, 299, 753, 807, 813
TaqII GACCGA 1 cut(s) 170
TasI AATT 5 cut(s) 212, 238, 398, 512, 802
TauI GCSGC 3 cut(s) 53, 144, 671
TfiI GAWTC 4 cut(s) 158, 296, 345, 847
Tru1I TTAA 3 cut(s) 396, 507, 732
Tru9I TTAA 3 cut(s) 396, 507, 732
TscAI CASTG 1 cut(s) 829
TseFI GTSAC 2 cut(s) 724, 820
TseI GCWGC 4 cut(s) 47, 124, 288, 419
Tsp45I GTSAC 2 cut(s) 724, 820
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 1 cut(s) 829
VpaK11BI GGWCC 2 cut(s) 380, 589
XapI RAATTY 1 cut(s) 398
XmiI GTMKAC 3 cut(s) 152, 258, 833
XspI CTAG 5 cut(s) 42, 243, 306, 384, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.