Rroxscaffold_2G00124000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017936
Physical Location & Seq
Reverse (-)
58054415 .. 58054777
363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00124000.1

Sequence Viewer

Length: 363 bp
ATGGCGGCAGAAGCACCATCTTCTTCTTCATCACGTAGCAATGTTTATCATCAAGGCTCCGACGACAAGGCCTTGACAGAGTCGGACTTGGCCGCCGCACAGCAGCTGATGCAACTCAGCGACGAGGATAACAACAACAGCAGCAGCAGTTGCGGCAGCAAGAAGAGAAGAAGTTACAAAGGGGAAGAGTACGAGACAGATGAAGAAGGGGTCGAATATCAGAGCCCGCTGAGTGTGATCACCTGCGCAAAGATTGAAGAGATTTTTGGGAAGGAAGACGAACAAGATTACCAGCCAAAGAAGAAGACAAGGTACCGGTCGCTTGCTGATATTTATCTGACGACCAGACCAATCAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

13.47

Weight (kDa)

4.84

Isoelectric Point (pI)

76.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017936)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09876
fragaria_vesca FvH4_1g23900
prunus_persica Prupe.6G161200_v2.0.a1
pyrus_communis pycom01g03560
rosa_laevigata RLG00000018498
rosa_roxburghii Rroxscaffold_2G00124000
rosa_rugosa Rorug02G0224600
rosa_samantha Rh2AG282200 Rh2BG293500 Rh2DG288400 Rh2DG307500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 251
Acc16I TGCGCA 1 cut(s) 247
Acc36I ACCTGC 1 cut(s) 251
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AciI CCGC 5 cut(s) 5, 93, 96, 153, 227
AcoI YGGCCR 1 cut(s) 90
AfaI GTAC 2 cut(s) 191, 314
AgeI ACCGGT 1 cut(s) 315
AgsI TTSAA 1 cut(s) 257
AjuI GAANNNNNNNTTGG 2 cut(s) 249, 281
AluBI AGCT 1 cut(s) 106
AluI AGCT 1 cut(s) 106
Alw26I GTCTC 1 cut(s) 188
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 2 cut(s) 69, 90
ApeKI GCWGC 4 cut(s) 103, 141, 144, 156
AsiGI ACCGGT 1 cut(s) 315
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 232
BanI GGYRCC 1 cut(s) 312
BanII GRGCYC 1 cut(s) 227
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbsI GAAGAC 2 cut(s) 282, 311
BbvI GCAGC 4 cut(s) 115, 153, 156, 168
BccI CCATC 1 cut(s) 25
BclI TGATCA 1 cut(s) 237
BcoDI GTCTC 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 251
BisI GCNGC 8 cut(s) 6, 93, 96, 104, 142, 145, 154, 157
BlsI GCNGC 8 cut(s) 7, 94, 97, 105, 143, 146, 155, 158
BmiI GGNNCC 2 cut(s) 58, 314
BmsI GCATC 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 282, 311
BsaAI YACGTR 1 cut(s) 35
BsaBI GATNNNNATC 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 315
Bse118I RCCGGY 1 cut(s) 315
Bse3DI GCAATG 1 cut(s) 46
Bse8I GATNNNNATC 1 cut(s) 333
BseJI GATNNNNATC 1 cut(s) 333
BseMI GCAATG 1 cut(s) 46
BseMII CTCAG 2 cut(s) 130, 221
BseXI GCAGC 4 cut(s) 115, 153, 156, 168
Bsh1285I CGRYCG 1 cut(s) 320
BshFI GGCC 2 cut(s) 71, 92
BshNI GGYRCC 1 cut(s) 312
BshTI ACCGGT 1 cut(s) 315
BsiEI CGRYCG 1 cut(s) 320
BsiSI CCGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 188
BsnI GGCC 2 cut(s) 71, 92
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 1 cut(s) 237
BspACI CCGC 5 cut(s) 5, 93, 96, 153, 227
BspANI GGCC 2 cut(s) 71, 92
BspCNI CTCAG 2 cut(s) 129, 222
BspLI GGNNCC 2 cut(s) 58, 314
BspMI ACCTGC 1 cut(s) 251
BspT107I GGYRCC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 46
BsrFI RCCGGY 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 315
BssMI GATC 1 cut(s) 237
Bst6I CTCTTC 3 cut(s) 158, 180, 252
BstAPI GCANNNNNTGC 2 cut(s) 109, 150
BstBAI YACGTR 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 227, 324
BstDEI CTNAG 2 cut(s) 116, 230
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 1 cut(s) 240
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 1 cut(s) 237
BstMCI CGRYCG 1 cut(s) 320
BstMWI GCNNNNNNNGC 4 cut(s) 11, 109, 150, 153
BstV1I GCAGC 4 cut(s) 115, 153, 156, 168
BstV2I GAAGAC 2 cut(s) 282, 311
BsuRI GGCC 2 cut(s) 71, 92
BveI ACCTGC 1 cut(s) 251
Cac8I GCNNGC 2 cut(s) 227, 324
CaiI CAGNNNCTG 1 cut(s) 106
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 1 cut(s) 315
Csp6I GTAC 2 cut(s) 190, 313
CspAI ACCGGT 1 cut(s) 315
CviAII CATG 1 cut(s) 360
CviJI RGCY 6 cut(s) 57, 71, 92, 106, 225, 295
CviKI_1 RGCY 6 cut(s) 57, 71, 92, 106, 225, 295
CviQI GTAC 2 cut(s) 190, 313
DdeI CTNAG 2 cut(s) 116, 230
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 3 cut(s) 158, 180, 252
EarI CTCTTC 3 cut(s) 158, 180, 252
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 1 cut(s) 363
FaiI YATR 1 cut(s) 361
FatI CATG 1 cut(s) 359
FauI CCCGC 1 cut(s) 234
FbaI TGATCA 1 cut(s) 237
Fnu4HI GCNGC 8 cut(s) 6, 93, 96, 104, 142, 145, 154, 157
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 8 cut(s) 6, 93, 96, 104, 142, 145, 154, 157
FspI TGCGCA 1 cut(s) 247
GlaI GCGC 1 cut(s) 247
GluI GCNGC 8 cut(s) 6, 93, 96, 104, 142, 145, 154, 157
HaeIII GGCC 2 cut(s) 71, 92
HapII CCGG 1 cut(s) 316
HhaI GCGC 1 cut(s) 248
Hin1II CATG 1 cut(s) 363
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HinfI GANTC 1 cut(s) 80
HpaII CCGG 1 cut(s) 316
HphI GGTGA 1 cut(s) 232
Hpy188I TCNGA 5 cut(s) 61, 85, 222, 339, 356
Hpy99I CGWCG 2 cut(s) 65, 125
HpyAV CCTTC 2 cut(s) 200, 265
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 1 cut(s) 112
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 109, 150, 153
HpyF3I CTNAG 2 cut(s) 116, 230
HpySE526I ACGT 1 cut(s) 34
Hsp92II CATG 1 cut(s) 363
HspAI GCGC 1 cut(s) 246
KpnI GGTACC 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 237
Kzo9I GATC 1 cut(s) 237
LmnI GCTCC 1 cut(s) 62
LpnPI CCDG 4 cut(s) 256, 305, 329, 358
Lsp1109I GCAGC 4 cut(s) 115, 153, 156, 168
LweI GCATC 1 cut(s) 99
MaeII ACGT 1 cut(s) 34
MaeIII GTNAC 1 cut(s) 173
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MhlI GDGCHC 1 cut(s) 227
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 63, 84
MnlI CCTC 1 cut(s) 118
MspA1I CMGCKG 2 cut(s) 106, 229
MspI CCGG 1 cut(s) 316
MwoI GCNNNNNNNGC 4 cut(s) 11, 109, 150, 153
NdeII GATC 1 cut(s) 237
NlaIII CATG 1 cut(s) 363
NlaIV GGNNCC 2 cut(s) 58, 314
NsbI TGCGCA 1 cut(s) 247
PaqCI CACCTGC 1 cut(s) 251
PceI AGGCCT 1 cut(s) 71
PflFI GACNNNGTC 1 cut(s) 79
PinAI ACCGGT 1 cut(s) 315
PkrI GCNGC 8 cut(s) 7, 94, 97, 105, 143, 146, 155, 158
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
Ppu21I YACGTR 1 cut(s) 35
PspN4I GGNNCC 2 cut(s) 58, 314
PstNI CAGNNNCTG 1 cut(s) 106
PsyI GACNNNGTC 1 cut(s) 79
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 2 cut(s) 191, 314
RsaNI GTAC 2 cut(s) 190, 313
SatI GCNGC 8 cut(s) 6, 93, 96, 104, 142, 145, 154, 157
Sau3AI GATC 1 cut(s) 237
SchI GAGTC 1 cut(s) 89
SduI GDGCHC 1 cut(s) 227
SetI ASST 4 cut(s) 37, 108, 245, 314
SfaNI GCATC 1 cut(s) 99
SseBI AGGCCT 1 cut(s) 71
SsiI CCGC 5 cut(s) 5, 93, 96, 153, 227
StuI AGGCCT 1 cut(s) 71
TaiI ACGT 1 cut(s) 37
TaqI TCGA 1 cut(s) 213
TauI GCSGC 4 cut(s) 8, 95, 98, 156
TseI GCWGC 4 cut(s) 103, 141, 144, 156
TspDTI ATGAA 2 cut(s) 18, 216
Tth111I GACNNNGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.