Rroxscaffold_2G00140080

LYK5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016730
Physical Location & Seq
Forward (+)
78377134 .. 78379007
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140080.1

Sequence Viewer

Length: 1806 bp
ATGGCAGCCTCCCTCCCATGTTCATCAATAGTCTGTATTCTGCTACTGTTCTTCTTCACCACTCAAATTTTAGCCCAGCCAGTACCACAGTCGAACTCGACCACAAACTTTTCATGTGCAGTAGATGCACCATCTTCATGCGAAACATATATAACTTACTTTGCACGGTCTCCAGGGTATATGAACCTGGAAAACATATCTGATCTATTTGGGGTCAGTGTCTCATCAATTGCCAAGGCCAGTAATCTAGCTTCCAGTTACACAGGTCAGACCCGATTGGTTGCAGGCCAACTCTTACTAGTACCTATCACCTGTGGTTGTACTGGAAACCGGTCTTTTGCAAACATTACCTATCTGATCAAGAGTGGAGATAGCTACTACGTGGTTTCGATCAATACATTTGAGAATCTCACCAAATGGCAGTTAGTGCAAGAGATGAATCCAGATCTGATTCCCACCCACTTGCAGATTGGTGACAAAGTGATTTTTCCTTTGTTCTGCAAATGTCCCTCGAAGACGAACTCAGATTTAATTCAGTATCTCATCACTTATGTCTGGCAGACTGATGATGACATCTTTAGAGTGGGTGCCAAGTTTAATGTTAAAGAATTTGAGATCTCAGACGCAAATAACCAGCGGGACTTCAGCAATTCAAGTGCAGTAGTGGGACAACCGGTGCTGATACCACTGACAGGGTTGCCTGCTCTATTCCAACCACTACCCCCTCATGGAAAACACAACTACCGGTTGATGCTTATTGTCATCATATGCTTGGGAGTTGCTCTATGTGTTGCTTCTCTTTTGGCAATTCTCGTGGTTTACACTCGTCGTCTGTGCAAGAGACAGAAGCTTTTGAATGGTAAGAGTTTGTCTCTGGAAAGCGCTGAATGGTTCCGAAAGAAAGAAGCAAAAAGTGAAGAGAAGATTGAGCTGAAGTTTGTACAGGACAAGCTTCTTCCTGGAGTTTCAAGCTATCTAGGAAAGGCAATCCTCTATGATATCAAAACCATTATGGAGGCAACCATGAATCTCAACGACCACAGTAGGATTGGAGGGTCTGTATACAGGGCTGTAATTGATGGGAAGGTCTTGGCTGTGAAGAAAACCAAGGAAGATGTCACTGAGGAACTAAATATCCTGCAGAAGGTAAATCATGCAAATCTGGTGAAACTTATGGGCATCTCATCTGAGATCGACGGGGATCGCTTCTTGGTTTATGAATATGCTGAAAATGGATCACTTGATAAATGGTTGCAGTATAAATCTTCAACCAGTTCAAGTTCTGGGGCATTCCTCACATGGAGTCAGAGATTAAGCATAGCTCTAGATGTTGCCAATGGCCTGCAATACATGCATGAACACACGCAACCGAGCATCGTTCACATGGATATAAGGACAAGTAACATACTTCTGGACTCCAAATACAAGGCCAAGATAGCAAACTTCTCCATGGCTAGAACTGCTGCAAACAGTGTTACACCAAAGGCGGATGTTTTCTCTTTCGGTGTTGTTCTGTTGTCTTTGCTTTCGGGGAAGAAAGGCATGGAAACAAAAGAGAATGGCGAGGTCATCATGTTGTGGAAGGATGTTAGGGGGTTTTGGAAGCTGAAGAGAAAAAACAGGAGAGCCTTGGCAAGCGCATGCACACAGGAAAAGTCATCAGCAAGACCAAGCATGGCAGAAGTTGTCTTCAACCTCTCTGTTCTTACTCATTCATCGTCTGAGTCGACACTGGAGAGGTCTTGGAATTCTGCATTGGAAGCAGAAGAAGTCCTTCAAACTATCACTCCTATCAGAGCTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002237 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005775 GO:0005886 GO:0006464 GO:0006468 GO:0006486 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009605 GO:0009607 GO:0009617 GO:0009877 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0032101 GO:0032103 GO:0034645 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043413 GO:0043900 GO:0043902 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070013 GO:0070085 GO:0071704 GO:0071944 GO:0080134 GO:0140096 GO:1900150 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1902288 GO:1902290
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

601

Amino Acids

66.72

Weight (kDa)

8.86

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM1_NFP_LYK PF23446 46 - 108 4.4e-22 NFP/LYK LysM1 domain
LysM2_NFP PF23457 112 - 166 1.3e-26 NFP LysM2 domain
LysM2_CERK1_LYK3_4_5 PF23472 114 - 165 1.7e-08 LYK3/4/5 LysM2 domain
LysM3_NFP PF23462 180 - 229 1.4e-16 NFP LysM3 domain
Pkinase PF00069 362 - 491 6.6e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 367 - 489 9.3e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016730)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14270
malus_domestica MD02G1156500.v1.1
prunus_persica Prupe.7G147300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0102941
rosa_laevigata RLG00000017189
rosa_multiflora Rmu_sc0000388.1_g000044
rosa_roxburghii Rroxscaffold_2G00140080 Rroxscaffold_2G00140210
rosa_rugosa Rorug02G0110500
rosa_samantha Rh2AG158800 Rh2BG165200 Rh2CG165400 Rh2DG164000
rosa_wichuraiana Rw2G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 587
AccI GTMKAC 2 cut(s) 1062, 1727
AciI CCGC 2 cut(s) 637, 1487
AclWI GGATC 2 cut(s) 1209, 1243
AcsI RAATTY 3 cut(s) 66, 608, 1747
AcuI CTGAAG 3 cut(s) 628, 953, 1628
AfaI GTAC 4 cut(s) 84, 303, 322, 942
AfeI AGCGCT 1 cut(s) 883
AfiI CCNNNNNNNGG 3 cut(s) 692, 728, 1144
AgeI ACCGGT 3 cut(s) 330, 673, 744
AgsI TTSAA 7 cut(s) 654, 856, 969, 1269, 1278, 1693, 1778
AhlI ACTAGT 1 cut(s) 298
AjnI CCWGG 3 cut(s) 172, 186, 958
AjuI GAANNNNNNNTTGG 2 cut(s) 1739, 1771
AloI GAACNNNNNNTCC 2 cut(s) 1119, 1151
AluBI AGCT 9 cut(s) 251, 375, 850, 931, 952, 972, 1322, 1606, 1799
AluI AGCT 9 cut(s) 251, 375, 850, 931, 952, 972, 1322, 1606, 1799
Alw21I GWGCWC 1 cut(s) 1801
Alw26I GTCTC 4 cut(s) 174, 226, 835, 876
AlwI GGATC 2 cut(s) 1209, 1243
Aor51HI AGCGCT 1 cut(s) 883
AoxI GGCC 4 cut(s) 237, 286, 1339, 1428
ApeKI GCWGC 2 cut(s) 5, 1463
ApoI RAATTY 3 cut(s) 66, 608, 1747
AsiGI ACCGGT 3 cut(s) 330, 673, 744
Asp700I GAANNNNTTC 1 cut(s) 1773
AspLEI GCGC 2 cut(s) 884, 1640
AsuHPI GGTGA 5 cut(s) 49, 301, 403, 485, 1177
BanI GGYRCC 1 cut(s) 587
BanII GRGCYC 1 cut(s) 1801
BarI GAAGNNNNNNTAC 2 cut(s) 235, 267
BauI CACGAG 1 cut(s) 812
BbsI GAAGAC 2 cut(s) 521, 1681
Bbv12I GWGCWC 1 cut(s) 1801
BbvI GCAGC 2 cut(s) 17, 1450
BccI CCATC 2 cut(s) 139, 1073
BciT130I CCWGG 3 cut(s) 174, 188, 960
BclI TGATCA 1 cut(s) 357
BcoDI GTCTC 4 cut(s) 174, 226, 835, 876
BcuI ACTAGT 1 cut(s) 298
BfaI CTAG 5 cut(s) 248, 299, 977, 1325, 1455
BfmI CTRYAG 1 cut(s) 1139
BfoI RGCGCY 1 cut(s) 885
BglII AGATCT 2 cut(s) 445, 615
BisI GCNGC 2 cut(s) 6, 1464
BlsI GCNGC 2 cut(s) 7, 1465
Bme1390I CCNGG 3 cut(s) 174, 188, 960
BmiI GGNNCC 2 cut(s) 589, 893
BmrFI CCNGG 3 cut(s) 174, 188, 960
BmsI GCATC 4 cut(s) 115, 741, 1188, 1383
BpiI GAAGAC 2 cut(s) 521, 1681
BplI GAGNNNNNCTC 2 cut(s) 856, 888
BpmI CTGGAG 3 cut(s) 156, 981, 1754
BsaAI YACGTR 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 5 cut(s) 173, 234, 1107, 1449, 1629
BsaWI WCCGGW 3 cut(s) 330, 673, 744
BsaXI ACNNNNNCTCC 4 cut(s) 1044, 1074, 1771, 1801
Bsc4I CCNNNNNNNGG 3 cut(s) 692, 728, 1144
Bse118I RCCGGY 3 cut(s) 330, 673, 744
Bse1I ACTGG 6 cut(s) 80, 240, 255, 328, 1272, 1737
BseBI CCWGG 3 cut(s) 174, 188, 960
BseDI CCNNGG 5 cut(s) 173, 234, 1107, 1449, 1629
BseGI GGATG 2 cut(s) 1495, 1591
BseLI CCNNNNNNNGG 3 cut(s) 692, 728, 1144
BseMII CTCAG 5 cut(s) 537, 633, 1113, 1179, 1713
BseNI ACTGG 6 cut(s) 80, 240, 255, 328, 1272, 1737
BseXI GCAGC 2 cut(s) 17, 1450
BseYI CCCAGC 1 cut(s) 75
BsgI GTGCAG 2 cut(s) 138, 678
BshFI GGCC 4 cut(s) 239, 288, 1341, 1430
BshNI GGYRCC 1 cut(s) 587
BshTI ACCGGT 3 cut(s) 330, 673, 744
BsiHKAI GWGCWC 1 cut(s) 1801
BsiSI CCGG 3 cut(s) 331, 674, 745
BslFI GGGAC 3 cut(s) 492, 653, 681
BslI CCNNNNNNNGG 3 cut(s) 692, 728, 1144
BsmAI GTCTC 4 cut(s) 174, 226, 835, 876
BsmFI GGGAC 3 cut(s) 492, 653, 681
BsmI GAATGC 1 cut(s) 1289
BsnI GGCC 4 cut(s) 239, 288, 1341, 1430
Bso31I GGTCTC 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 1801
Bsp1407I TGTACA 1 cut(s) 940
Bsp143I GATC 8 cut(s) 202, 357, 390, 445, 615, 1191, 1201, 1235
Bsp19I CCATGG 1 cut(s) 1449
BspACI CCGC 2 cut(s) 637, 1487
BspANI GGCC 4 cut(s) 239, 288, 1341, 1430
BspCNI CTCAG 5 cut(s) 536, 632, 1114, 1180, 1714
BspLI GGNNCC 2 cut(s) 589, 893
BspMAI CTGCAG 1 cut(s) 1143
BspPI GGATC 2 cut(s) 1209, 1243
BspT107I GGYRCC 1 cut(s) 587
BspTNI GGTCTC 1 cut(s) 174
BsrFI RCCGGY 3 cut(s) 330, 673, 744
BsrGI TGTACA 1 cut(s) 940
BsrI ACTGG 6 cut(s) 80, 240, 255, 328, 1272, 1737
BssAI RCCGGY 3 cut(s) 330, 673, 744
BssECI CCNNGG 5 cut(s) 173, 234, 1107, 1449, 1629
BssMI GATC 8 cut(s) 202, 357, 390, 445, 615, 1191, 1201, 1235
BssNAI GTATAC 1 cut(s) 1063
BssSI CACGAG 1 cut(s) 812
BssT1I CCWWGG 4 cut(s) 234, 1107, 1449, 1629
Bst1107I GTATAC 1 cut(s) 1063
Bst2BI CACGAG 1 cut(s) 812
Bst2UI CCWGG 3 cut(s) 174, 188, 960
Bst4CI ACNGT 5 cut(s) 48, 90, 168, 1043, 1472
Bst6I CTCTTC 2 cut(s) 912, 1604
BstAPI GCANNNNNTGC 3 cut(s) 125, 427, 1351
BstAUI TGTACA 1 cut(s) 940
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 6 cut(s) 286, 702, 1343, 1636, 1642, 1801
BstDEI CTNAG 5 cut(s) 523, 619, 1122, 1188, 1722
BstDSI CCRYGG 1 cut(s) 1449
BstENI CCTNNNNNAGG 1 cut(s) 1142
BstF5I GGATG 2 cut(s) 1495, 1591
BstH2I RGCGCY 1 cut(s) 885
BstHHI GCGC 2 cut(s) 884, 1640
BstKTI GATC 8 cut(s) 205, 360, 393, 448, 618, 1194, 1204, 1238
BstMAI GTCTC 4 cut(s) 174, 226, 835, 876
BstMBI GATC 8 cut(s) 202, 357, 390, 445, 615, 1191, 1201, 1235
BstMWI GCNNNNNNNGC 6 cut(s) 125, 427, 1351, 1436, 1460, 1760
BstNI CCWGG 3 cut(s) 174, 188, 960
BstNSI RCATGY 2 cut(s) 1354, 1644
BstSCI CCNGG 3 cut(s) 172, 186, 958
BstSFI CTRYAG 1 cut(s) 1139
BstV1I GCAGC 2 cut(s) 17, 1450
BstV2I GAAGAC 2 cut(s) 521, 1681
BstX2I RGATCY 2 cut(s) 445, 615
BstYI RGATCY 2 cut(s) 445, 615
BstZ17I GTATAC 1 cut(s) 1063
BsuRI GGCC 4 cut(s) 239, 288, 1341, 1430
BtgI CCRYGG 1 cut(s) 1449
BtsCI GGATG 2 cut(s) 1495, 1591
BtsIMutI CAGTG 5 cut(s) 223, 686, 1119, 1477, 1730
Cac8I GCNNGC 6 cut(s) 286, 702, 1343, 1636, 1642, 1801
CfoI GCGC 2 cut(s) 884, 1640
Cfr10I RCCGGY 3 cut(s) 330, 673, 744
CseI GACGC 1 cut(s) 632
Csp6I GTAC 4 cut(s) 83, 302, 321, 941
CspAI ACCGGT 3 cut(s) 330, 673, 744
CspCI CAANNNNNGTGG 2 cut(s) 795, 830
CviQI GTAC 4 cut(s) 83, 302, 321, 941
DdeI CTNAG 5 cut(s) 523, 619, 1122, 1188, 1722
DpnI GATC 8 cut(s) 204, 359, 392, 447, 617, 1193, 1203, 1237
DpnII GATC 8 cut(s) 202, 357, 390, 445, 615, 1191, 1201, 1235
Eam1104I CTCTTC 2 cut(s) 912, 1604
EarI CTCTTC 2 cut(s) 912, 1604
EciI GGCGGA 1 cut(s) 1502
Ecl136II GAGCTC 1 cut(s) 1799
Eco130I CCWWGG 4 cut(s) 234, 1107, 1449, 1629
Eco24I GRGCYC 1 cut(s) 1801
Eco31I GGTCTC 1 cut(s) 174
Eco32I GATATC 1 cut(s) 1000
Eco47III AGCGCT 1 cut(s) 883
Eco53kI GAGCTC 1 cut(s) 1799
Eco57I CTGAAG 3 cut(s) 628, 953, 1628
EcoICRI GAGCTC 1 cut(s) 1799
EcoNI CCTNNNNNAGG 1 cut(s) 1142
EcoRI GAATTC 1 cut(s) 1747
EcoRII CCWGG 3 cut(s) 172, 186, 958
EcoRV GATATC 1 cut(s) 1000
EcoT14I CCWWGG 4 cut(s) 234, 1107, 1449, 1629
EcoT22I ATGCAT 1 cut(s) 1356
EcoT38I GRGCYC 1 cut(s) 1801
ErhI CCWWGG 4 cut(s) 234, 1107, 1449, 1629
FalI AAGNNNNNCTT 2 cut(s) 1758, 1790
FaqI GGGAC 3 cut(s) 492, 653, 681
FauI CCCGC 1 cut(s) 630
FauNDI CATATG 1 cut(s) 767
FbaI TGATCA 1 cut(s) 357
FblI GTMKAC 2 cut(s) 1062, 1727
Fnu4HI GCNGC 2 cut(s) 6, 1464
FokI GGATG 2 cut(s) 1502, 1598
FriOI GRGCYC 1 cut(s) 1801
Fsp4HI GCNGC 2 cut(s) 6, 1464
FspBI CTAG 5 cut(s) 248, 299, 977, 1325, 1455
GlaI GCGC 2 cut(s) 883, 1639
GluI GCNGC 2 cut(s) 6, 1464
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 3 cut(s) 156, 981, 1754
HaeII RGCGCY 1 cut(s) 885
HaeIII GGCC 4 cut(s) 239, 288, 1341, 1430
HapII CCGG 3 cut(s) 331, 674, 745
HgaI GACGC 1 cut(s) 632
HhaI GCGC 2 cut(s) 884, 1640
Hin6I GCGC 2 cut(s) 882, 1638
HinP1I GCGC 2 cut(s) 882, 1638
HincII GTYRAC 1 cut(s) 1728
HindII GTYRAC 1 cut(s) 1728
HindIII AAGCTT 2 cut(s) 848, 950
HinfI GANTC 7 cut(s) 406, 439, 451, 1027, 1303, 1415, 1724
HpaII CCGG 3 cut(s) 331, 674, 745
HphI GGTGA 5 cut(s) 49, 301, 403, 485, 1177
Hpy166II GTNNAC 4 cut(s) 820, 1063, 1381, 1728
Hpy188III TCNNGA 5 cut(s) 361, 443, 875, 1325, 1412
Hpy8I GTNNAC 4 cut(s) 820, 1063, 1381, 1728
Hpy99I CGWCG 2 cut(s) 831, 1199
HpyAV CCTTC 4 cut(s) 1078, 1138, 1576, 1784
HpyCH4III ACNGT 5 cut(s) 48, 90, 168, 1043, 1472
HpyCH4IV ACGT 1 cut(s) 381
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 427, 1351, 1436, 1460, 1760
HpyF3I CTNAG 5 cut(s) 523, 619, 1122, 1188, 1722
HpySE526I ACGT 1 cut(s) 381
HspAI GCGC 2 cut(s) 882, 1638
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 8 cut(s) 202, 357, 390, 445, 615, 1191, 1201, 1235
Lsp1109I GCAGC 2 cut(s) 17, 1450
LweI GCATC 4 cut(s) 115, 741, 1188, 1383
MaeI CTAG 5 cut(s) 248, 299, 977, 1325, 1455
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 5 cut(s) 257, 473, 1117, 1400, 1474
MalI GATC 8 cut(s) 204, 359, 392, 447, 617, 1193, 1203, 1237
MboI GATC 8 cut(s) 202, 357, 390, 445, 615, 1191, 1201, 1235
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 2 cut(s) 445, 615
MhlI GDGCHC 1 cut(s) 1801
MluCI AATT 8 cut(s) 66, 228, 531, 608, 649, 807, 1074, 1747
MlyI GAGTC 3 cut(s) 1312, 1409, 1733
MmeI TCCRAC 1 cut(s) 736
Mph1103I ATGCAT 1 cut(s) 1356
MroXI GAANNNNTTC 1 cut(s) 1773
MseI TTAA 4 cut(s) 530, 597, 603, 1313
MslI CAYNNNNRTG 1 cut(s) 136
MspA1I CMGCKG 1 cut(s) 637
MspI CCGG 3 cut(s) 331, 674, 745
MspR9I CCNGG 3 cut(s) 174, 188, 960
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 1 cut(s) 1289
MvaI CCWGG 3 cut(s) 174, 188, 960
MwoI GCNNNNNNNGC 6 cut(s) 125, 427, 1351, 1436, 1460, 1760
NcoI CCATGG 1 cut(s) 1449
NdeI CATATG 1 cut(s) 767
NdeII GATC 8 cut(s) 202, 357, 390, 445, 615, 1191, 1201, 1235
NlaIV GGNNCC 2 cut(s) 589, 893
NmuCI GTSAC 2 cut(s) 473, 1117
NsiI ATGCAT 1 cut(s) 1356
NspI RCATGY 2 cut(s) 1354, 1644
PaeI GCATGC 1 cut(s) 1644
PcsI WCGNNNNNNNCGW 1 cut(s) 1724
PctI GAATGC 1 cut(s) 1289
PdmI GAANNNNTTC 1 cut(s) 1773
PfeI GAWTC 4 cut(s) 406, 439, 451, 1027
PfoI TCCNGGA 1 cut(s) 958
PinAI ACCGGT 3 cut(s) 330, 673, 744
PkrI GCNGC 2 cut(s) 7, 1465
PleI GAGTC 3 cut(s) 1311, 1409, 1732
PpsI GAGTC 3 cut(s) 1311, 1409, 1732
Ppu21I YACGTR 1 cut(s) 382
Psp124BI GAGCTC 1 cut(s) 1801
Psp6I CCWGG 3 cut(s) 172, 186, 958
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 3 cut(s) 172, 186, 958
PspN4I GGNNCC 2 cut(s) 589, 893
PstI CTGCAG 1 cut(s) 1143
PsuI RGATCY 2 cut(s) 445, 615
RsaI GTAC 4 cut(s) 84, 303, 322, 942
RsaNI GTAC 4 cut(s) 83, 302, 321, 941
RseI CAYNNNNRTG 1 cut(s) 136
SacI GAGCTC 1 cut(s) 1801
SalI GTCGAC 1 cut(s) 1726
SaqAI TTAA 4 cut(s) 530, 597, 603, 1313
SatI GCNGC 2 cut(s) 6, 1464
Sau3AI GATC 8 cut(s) 202, 357, 390, 445, 615, 1191, 1201, 1235
SchI GAGTC 3 cut(s) 1312, 1409, 1733
ScrFI CCNGG 3 cut(s) 174, 188, 960
SduI GDGCHC 1 cut(s) 1801
SfaNI GCATC 4 cut(s) 115, 741, 1188, 1383
SfcI CTRYAG 1 cut(s) 1139
SmiMI CAYNNNNRTG 1 cut(s) 136
SpeI ACTAGT 1 cut(s) 298
SphI GCATGC 1 cut(s) 1644
Sse9I AATT 8 cut(s) 66, 228, 531, 608, 649, 807, 1074, 1747
SsiI CCGC 2 cut(s) 637, 1487
SspMI CTAG 5 cut(s) 248, 299, 977, 1325, 1455
SstI GAGCTC 1 cut(s) 1801
StyD4I CCNGG 3 cut(s) 172, 186, 958
StyI CCWWGG 4 cut(s) 234, 1107, 1449, 1629
TaaI ACNGT 5 cut(s) 48, 90, 168, 1043, 1472
TaiI ACGT 1 cut(s) 384
TaqI TCGA 6 cut(s) 92, 98, 389, 512, 1194, 1727
TasI AATT 8 cut(s) 66, 228, 531, 608, 649, 807, 1074, 1747
TatI WGTACW 2 cut(s) 320, 940
TfiI GAWTC 4 cut(s) 406, 439, 451, 1027
Tru1I TTAA 4 cut(s) 530, 597, 603, 1313
Tru9I TTAA 4 cut(s) 530, 597, 603, 1313
TscAI CASTG 5 cut(s) 223, 693, 1126, 1477, 1737
TseFI GTSAC 2 cut(s) 473, 1117
TseI GCWGC 2 cut(s) 5, 1463
Tsp45I GTSAC 2 cut(s) 473, 1117
TspDTI ATGAA 9 cut(s) 12, 102, 126, 197, 452, 1040, 1233, 1371, 1704
TspRI CASTG 5 cut(s) 223, 693, 1126, 1477, 1737
XagI CCTNNNNNAGG 1 cut(s) 1142
XapI RAATTY 3 cut(s) 66, 608, 1747
XbaI TCTAGA 1 cut(s) 1324
XceI RCATGY 2 cut(s) 1354, 1644
XcmI CCANNNNNNNNNTGG 2 cut(s) 467, 1046
XmiI GTMKAC 2 cut(s) 1062, 1727
XmnI GAANNNNTTC 1 cut(s) 1773
XspI CTAG 5 cut(s) 248, 299, 977, 1325, 1455
Zsp2I ATGCAT 1 cut(s) 1356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.